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991.
Seven studies were conducted in commercial grazing operations to confirm anthelmintic efficacy, assess acceptability, and measure the productivity response of cattle to treatment with eprinomectin in an extended-release injectable formulation (ERI) when exposed to nematode infected pastures for 120 days. The studies were conducted under one protocol in the USA in seven locations (Arkansas, Idaho, Louisiana, Minnesota, Missouri, Oregon, and Wisconsin). Each study had 67–68 naturally infected animals for a total of 475 (226 female, 249 male castrate) Angus or beef-cross cattle. The animals weighed 133–335 kg prior to treatment and were approximately 3–12 months of age. The studies were conducted under a randomized block design based on pre-treatment body weights to sequentially form 17 replicates of four animals each within sex in each study. Animals within a replicate were randomly assigned to treatments, one to Eprinomectin ERI vehicle (control) and three to Eprinomectin ERI (5%, w/v eprinomectin). Treatments were administered at 1 mL/50 kg body weight once subcutaneously anterior to the shoulder. All animals in each study grazed one pasture throughout the observation period of 120 days. Cattle were weighed and fecal samples collected pre-treatment and on 28, 56, 84, and 120 days after treatment for fecal egg and lungworm larval counts. Positive fecal samples generally were cultured en masse to determine the nematode genera attributable to the gastrointestinal helminth infection. Bunostomum, Cooperia, Haemonchus, Nematodirus, Oesophagostomum, Ostertagia, and Trichostrongylus, when present, were referred to as strongylids. At all post-treatment sampling intervals, Eprinomectin ERI-treated cattle had significantly (P < 0.05) lower strongylid egg counts than vehicle-treated controls, with ≥95% reduction after 120 days of grazing. Over this same period, Eprinomectin ERI-treated cattle gained more weight (43.9 lb/head) than vehicle-treated controls in all studies. This weight gain advantage was significant (P < 0.05) in six of the studies with the Eprinomectin ERI-treated cattle gaining an average of 42.8% and the control cattle gaining 33.1% of their initial weight. No adverse reactions were observed in the treated animals.  相似文献   
992.
Equine farms are increasing in number in Northeast and Mid-Atlantic states. These farms may influence environmental quality owing to manure collection, storage, spreading, and disposal practices. A manure management survey was mailed to 700 New Jersey equine farms during the winter of 2009-2010 (these farms were located in two watersheds). Two hundred forty-two surveys were returned; 10% were training or performance farms, whereas the remainder (90%) were breeding, boarding, or pleasure farms. There were differences between the two watersheds in turnout time, pasture acreage, use of drag harrows, and income. Respondents answered questions about diet and environmental management on farms. Seventy-eight percent had bunk management plans. Forty-seven percent disposed of waste feed in a manure pile, 35% left it on fields or paddocks, and 18% put it in a dumpster. Twenty-six percent engage a professional for feeding advice (21% veterinarians, 2% extension, 3% nutrition consultants). Most respondents did not have a regular feed management plan. Sixty-two percent of all farms spread manure on-farm. Of those who spread, 29% have >8.09 hectares (20 acres) for spreading. Ninety-six percent of respondents stored manure >100 ft from water or wetlands. Comparison of cross-tabulations indicates a strong relationship (P < .01) between rotational grazing and the use of a sacrifice or exercise area. Eighty-eight percent of respondents using sacrifice areas also rotationally graze. Results indicate future outreach programs should focus on feed management, manure storage location, rotational grazing, and the use of sacrifice areas as part of a pasture management program.  相似文献   
993.
The aim of the study was to compare three methods commonly used to determine the concentrations of bacterial protein in digesta collected from the terminal ileum of growing pigs that had been fed a casein‐based diet. The amounts of bacterial protein in terminal ileal digesta were determined using three different markers: 2.6‐diaminopimelic acid (DAPA) and the d ‐amino acids, d ‐aspartic acid (d ‐Asp) and d ‐glutamic acid (d ‐Glu). The effectiveness of each marker was compared against a control based on physical fractionation by centrifugation. The total bacterial protein concentrations derived from the markers d ‐Asp and d ‐Glu were significantly different (p = 0.05) to those calculated from DAPA and the control, but there was no difference between DAPA and the control. The percentage of bacterial nitrogen ranged from 40% to 52% dependent on the marker used. Bacterial protein expressed as a percentage of the total protein, ranged from 48% to 62%, a substantial proportion of which (12–28%) was derived from lysed bacterial cells. Statistical correlations between the estimation methods were low. Such poor correlation between the markers may be the result of random errors such as variance in the epimerization of the two d ‐amino acids during protein hydrolysis. DAPA was accepted as a reliable marker for determining microbial protein in ileal digesta.  相似文献   
994.
The development of antral ovarian follicles entails fluid accumulation, but the mechanisms regulating water flux are unknown. Aquaporins are small, integral membrane proteins facilitating passive movement of water, some of which are known to be regulated by steroid hormones. The aim of this study was to determine whether testosterone (T) influences water transport in porcine granulosa cells. To assess water movement, the swelling of granulosa cells when moved from isotonic (319 mOsm) to hypotonic (95 mOsm) medium was measured after 12‐hour pre‐incubation in the presence of either testosterone (T), the antiandrogen 2‐hydroxyflutamide (HF) or HF and T together. Pre‐incubation with T increased the swelling of granulosa cells (p < 0.01) and this was abolished by HF (p < 0.001). Neither T nor HF affected cells in isotonic medium (319 mOsm). The results indicate that T acting via intracellular androgen receptors increases water permeability of porcine granulosa cells, probably through the regulation of aquaporin activity.  相似文献   
995.
Extract

Sir,—I should like to make a request to interested veterinarians for live specimens of the rare condition in sheep — testicular feminization.  相似文献   
996.
There is little information regarding the presentation, biologic behaviour, treatment and prognosis in cats with chronic lymphocytic leukaemia (CLL), and further investigation is needed to characterize this disease in cats. The goal of this study was to describe the clinical presentation, response to treatment and prognosis of feline CLL. A multi‐institutional retrospective study of 18 cats diagnosed with CLL between 2000 and 2010 was performed. CLL was defined as the presence of a mature lymphocytosis (> 9000 lymphocytes µL?1) and confirmation of an immunophenotypically monomorphic or clonal lymphoid population. Each patient was required to also have at least one of the two following criteria: (1) concurrent cytopenia of at least one cell line and/or (2) >15% mature lymphocytes in the bone marrow. Data on signalment, history, clinical signs, clinicopathologic features and response to treatment were reviewed. Median age of the cats at initial presentation was 12.5 years (range: 5–20 years). The most common presenting complaint was chronic weight loss, which was present in 8/18 (44%) cats. Sixteen of 18 (89%) cats were treated with chlorambucil and prednisolone; four of these cats also received vincristine. Two (11%) cats were treated with multi‐agent injectable chemotherapy (L‐CHOP, l ‐asparaginase, cyclophosphamide, doxorubicin, vincristine, prednisolone). Eighty‐eight percent of cats evaluable for response achieved a complete (nine cats) or partial (six cats) remission. Median overall remission was 15.7 months (range: 1.3–22.8 months). The median overall survival in the 17 cats with follow‐up data was 14.4 months (range: 0.9–25.3 months). Results of this study suggest that CLL affects older‐aged cats and responds favourably to treatment with oral chlorambucil and prednisolone.  相似文献   
997.
Landscape Ecology - We undertook reconstructions of historical ponderosa pine forest structure and fire regimes across an entire landscape to expand understanding of spatial variability in forest...  相似文献   
998.
A summer application of nitrogen was used to induce the production of “ strong ” flowers for comparison with the " normal ” flowers produced on trees of Worcester Pearmain given only a spring application of nitrogen.

The flowers were given various pollination treatments to provide material for an assessment of flower quality. These included the following : auto and alio self-pollination and cross-pollination with diploid apple or pear at anthesis, cross-pollinations and self-pollinations at additional daily intervals subsequent to anthesis.

The stigmas of strong flowers remained receptive for a longer period than those of normal flowers.

After cross-pollination with a diploid apple, fertilization took place in 6-7 days. After self-pollination a typical incompatibility reaction was apparent. Pear pollen tubes showed an intermediate response.

Egg sacs in strong flowers continued to enlarge after those of corresponding normal flowers had ceased to grow. The fertilized ovules and embryo sacs of strong flowers showed a more rapid acceleration in growth rate than those of normal flowers.

Cell division in unfertilized ovules also continued for a longer period in strong flowers and appeared to be correlated with the behaviour of the egg sac.

Ovule longevity provided the most striking difference between the two flower types. The ovules of strong flowers remained capable of fertilization for almost twice as long as those of normal flowers.

Abnormalities of several types were seen in ovules. The most common was the production of secondary egg sacs, a feature of strong flowers which had been self-pollinated. Three types of secondary sac, micropylar, lateral and chalazal are described and illustrated.

The effects of rate of pollen tube growth, ovule longevity and the period of stigma receptivity in limiting the effective pollination period, are discussed. It is suggested that some variations in fruit set may be due to differences in flower quality. The use of summer or early autumn nitrogen is advocated as a method of improving blossom quality in certain varieties.  相似文献   
999.
Summary

Grapevines, cv. Thompson Seedless were treated with the plant growth regulator paclobutrazol in the spring of 1984. The treatments consisted of four soil application rates (including a zero control) of paclobutrazol on two phenological dates (budbreak and bloom). Vegetative growth, as measured by pruning weight, vine yield and fruit maturity characteristics, were determined in 1984 and in the next three growing seasons. There was no significant difference in vine yield between treatments at harvest in 1984; however, the fruit of vines receiving paclobutrazol at budbreak had accumulated less sugar at that time. Paclobutrazol application significantly (P < 0.01 ) reduced pruning weights in 1984 for all vines receiving paclobutrazol. Only the highest rate of paclobutrazol significantly reduced pruning weights in 1985. The number of clusters per vine and yield were significantly less for the treated vines than for the control ones in 1985 and 1986. Pruning weights in 1986 and yield in 1987 showed no significant differences between treatments. The results indicate that a single soil application of paclobutrazol was effective in reducing vegetative growth of Thompson Seedless grapevines for a period of two years if the rate of application was greater than 1.1 kg a. i. ha-1. However, the adverse effect of this growth regulator on vine yield indicate that it would be of little practical use when applied via the soil to this seedless cultivar.  相似文献   
1000.

Microbiotic crusts occur extensively in rangeland soils . Developed by filaments of cyanobacteria and algae , and thalli of lichen and moss entanglement of soil particles , they create a physical discontinuity in the surface profile with greater concen trations of clay , silt , and potentially hydrophobic organic matter. These conditions potentially contribute to variability in soil hydrology of arid land and should be considered in the development of hydrologic and erosion models. However, there is limited manipulative research examining the functional relationships between soil and microbiotic crusts . We investigated the influence of cyanobacterial-dominated microbiotic crust on measured hydraulic conductivity (K) in a sandy loam soil at a southeastern Utah site . Using a tension infiltrometer , we determined K under three surface treatments: undisturbed , chemically killed (representing dead microphytes within the crust), and removed (scalped) microbiotic crusts. We applied treatments to spatially interspersed intact surface soils within shrub interspaces . Microbiotic crusts at this site and in this stage of successional development had no discernible influence on K. This finding supports results from research conducted in a variety of soils from sandy to silt dominated with a range of microbiotic development . Because this research was site and time specific , and because the role of microbiotic crusts in the environment continues to be debated , additional research is warranted to deter mine how stage of development of microbiotic crust influences soil hydrology .  相似文献   
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