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21.
22.
利用PCR技术和基因重组技术构建了枯草芽孢杆菌蛋白酶基因—apr的重组表达质粒PET-22b/apr,并将其转入大肠杆菌(E.coli)BL21中,经蓝白斑筛选获得apr的表达载体。PCR后经琼脂糖凝胶电泳结果显示,在约1.5kb位置有特异性条带,与预期的枯草芽孢杆菌蛋白酶基因大小一致。重组质粒pGM-T/apr经双酶切后获得约1509bp的apr基因片段,同预期结果相一致,测序结果同GeneBank中的序列同源性达到100%。未发生任何突变。表明重组质粒pET-22b/apr构建成功。 相似文献
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24.
ABSTRACT: The present study reports the annual variation in consumption of the sea urchin Strongylocentrotus intermedius by avian predators on a rocky shore where the culture of sea urchins has been conducted. Carrion crow and a few gull species were the most abundant avian predators and consumed a large number of sea urchins. Crows consumed mostly natural sea urchins, approximately 36 kg ww/ha per year on the intertidal rocky bench, but the gull species consumed mostly cultured sea urchins, approximately 100 kg ww/ha per year in the culture area. The seasonal variation in the amount of sea urchins consumed by crows was higher than that by the gull species, presumably because of the difference in foraging behavior in association with the seasonal tidal cycle. The natural sea urchins consumed are an allochthonous input from the subtidal to the intertidal habitat, and thus, crow predation may not affect the natural and the cultured populations of the sea urchin. The gull species consumed much of the cultured sea urchin, and thus, may be regarded as an effective predator causing damage to sea urchin culture. The results suggest that further studies are needed to determine why the gull species selectively feed on cultured sea urchins. 相似文献
25.
Sung Il Lee Kerim Y. Aydin Paul D. Spencer Thomas K. Wilderbuer Chang Ik Zhang 《Fisheries Science》2010,76(3):411-434
We evaluated the role of flatfishes in the organization and structure of the eastern Bering Sea ecosystem using the Ecopath/Ecosim
approach. As basic input data for the Ecopath/Ecosim model, we used estimates of biomass from bottom trawl surveys and age-structured
population models, production/biomass (P/B) ratio, consumption/biomass (Q/B) ratio, diet composition (DC), and fisheries harvests for each component of species or species groups. We estimated the trophic
level of each component, niche overlaps among flatfishes, and the impacts of competition and predation on flatfish species
in the eastern Bering Sea ecosystem. Based on those estimates, we developed the tropho-dynamic structure of the ecosystem,
and the model was used to simulate ecological effects of fishery exploitation patterns. No single flatfish species appeared
to have a profound and uniquely important role in the organization and structure of the ecosystem. Instead, the most important
component among the guild of flatfish species appeared to be yellowfin sole Pleuronectes asper, which had greater biomass than other flatfish and a relatively diverse diet among the small flatfish species. Pacific halibut
Hippoglossus stenolepis, Greenland turbot Reinhardtius hippoglossoides, and arrowtooth flounder Atheresthes stomias were important keystone predators in the eastern Bering Sea ecosystem together with some groups of marine mammals and sea
birds. Intra flatfish complex cannibalism was not observed, however, substantial diet overlaps were common in the flatfish
guild system. 相似文献
26.
27.
One hundred and forty-seven Chinese barley varieties maintained at the Gene Bank of the National Barley Improvement Centre,
Zhejiang, and 84 progenies from these varieties were tested at the seedling stage for their reaction to 32 selected pathotypes
of Blumeria graminis f. sp. hordei. Eighteen resistance spectra were identified comprising single or combined resistances from eight known (Ml(Bw), Ml(Ch), Mla7, Mla8, Mla9, Mla13, MlaRu4 and Mlg) and six unknown resistance genes. The most frequent gene was Ml(Bw), which was found in 69 varieties and previously detected in only a few European winter barley varieties. The genes Mla8 and Ml(Ch) were also often present, but other resistance genes were rare. After inoculation, considerably fewer pathogen colonies were
observed in ‘Aiganqi’ and one line of ‘Tong5’. Twenty varieties were composed of lines with different resistance genes. China
is likely to be a region of origin of the genes Ml(Bw), Mla7, as well as three unknown genes found in original landraces and perhaps another three unknown genes detected in cultivars
bred in China. The resistances of varieties from the Zhejiang province and those originating from 11 other Chinese provinces
were quite different. Unfortunately, none of the varieties are promising sources of resistance to powdery mildew and China
does not seem to be a region suitable for identifying such sources. 相似文献
28.
Sarah I. Beldin Bruce A. Caldwell Phillip Sollins Elizabeth W. Sulzman Kate Lajtha Susan E. Crow 《Biology and Fertility of Soils》2007,43(6):837-841
The cation exchange capacity (CEC) of a soil depends on the type and amount of both mineral and organic surfaces. Previous
studies that have sought to determine the relative contribution of organic matter to total soil CEC have not addressed differences
in soil organic matter (SOM) composition that could lead to differences in CEC. The objectives of this study were (1) to compare
the CEC of two distinct SOM pools, the “light fraction (LF)” composed of particulate plant, animal, and microbial debris,
and the “heavy fraction (HF)” composed of mineral-bound organic matter; and (2) to examine the effects of differences in aboveground
vegetation on CEC. Soil samples were collected from four paired grassland/conifer sites within a single forested area and
density fractionated. LF CEC was higher in conifer soils than in grassland soils, but there was no evidence of an effect of
vegetation on CEC for the HF or bulk soil. LF CEC (but not HF CEC) correlated well with the C concentration in the fraction.
The mean CEC of both fractions (per kg fraction) exceeded that of the bulk soil; thus, when the LF and HF CEC were combined
mathematically by weighting values for each fraction in proportion to dry mass, the resulting value was nearly twice the measured
CEC of bulk soil. On a whole soil basis, the HF contributed on average 97% of the CEC of the whole soil, although this conclusion
must be tempered given the inflation of CEC values by the density fractionation procedure. 相似文献
29.
Long-term effect of chemical fertilizer,straw, and manure on labile organic matter fractions in a paddy soil 总被引:2,自引:0,他引:2
To assess the effect of long-term fertilization on labile organic matter fractions, we analyzed the C and N mineralization
and C and N content in soil, particulate organic matter (POM), light fraction organic matter (LFOM), and microbial biomass.
Results showed that fertilizer N decreased or did not affect the C and N amounts in soil fractions, except N mineralization
and soil total N. The C and N amounts in soil and its fractions increased with the application of fertilizer PK and rice straw.
Generally, there was no significant difference between fertilizer PK and rice straw. Furthermore, application of manure was
most effective in maintaining soil organic matter and labile organic matter fractions. Soils treated with manure alone had
the highest microbial biomass C and C and N mineralization. A significant correlation was observed between the C content and
N content in soil, POM, LFOM, microbial biomass, or the readily mineralized organic matter. The amounts of POM–N, LFOM–N,
POM–C, and LFOM–C closely correlated with soil organic C or total N content. Microbial biomass N was closely related to the
amounts of POM–N, LFOM–N, POM–C, and LFOM–C, while microbial biomass C was closely related to the amounts of POM–N, POM–C,
and soil total N. These results suggested that microbial biomass C and N closely correlated with POM rather than SOM. Carbon
mineralization was closely related to the amounts of POM–N, POM–C, microbial biomass C, and soil organic C, but no significant
correlation was detected between N mineralization with C or N amounts in soil and its fractions. 相似文献
30.
Cultivated tetraploid Gossypium hirsutum L. (Malvaceae) cotton has been characterized as having one flower at a primordia. In a strain (CSH-13) of Gossypium hirsutum cotton, four plants out of 103 had two to three double bolls or twin bolls/plant during 2003–2004 crop season at CICR regional station, Sirsa, Haryana, India. Progeny evaluation in 2004 and 2005 crop seasons indicated that plants raised from seeds harvested from double bolls/twin bolls produced plants bearing double bolls only and plant progeny raised from the seed of single bolls from these mutant plants produced two to three double bolls per plant similar to the parent. Progeny testing revealed that double boll formation is the result of spontaneous mutation and environment does not influence its expression. The mutant is early in maturity by 10–15 days, naked seeded and possesses comparable agronomic characters with normal plants. Another spontaneous mutant of Gossypium hirsutum having more than three appendages originating from primordia.: four bolls one leaf, three bolls one leaf, two bolls two leaves, one boll three leaves were obtained from the population of CISV-13 strain in crop season 2003. These appendage groups have been described as bicolor unit by Clemens Bayer, [Zur Infloreszenzmorphologie der Malvales. Dissertations Botany 212 (1994)] and cluster mutant by Russell and Luther [J Cott Sci 6 (2002) 115]. One to three bolls in a cluster or bicolor unit were observed without formation of seeds and lint. About one-third of the total bolls on the mutant plant were of this type. In progeny testing during 2004 and 2005 crop seasons, this mutant produced plants exclusively with more than three appendages revealing that plants identified in 2003 were due to spontaneous mutation. This mutant was also early and naked seeded. Many of the other characters of the mutant plants were comparable with the wild type plants of the strain. Both the mutants were observed having economic impact due to their better yielding ability as compare to respective parents. 相似文献