早花忍冬离体快繁技术

以东北地区早春植物早花忍冬的幼嫩茎段为外植体,MS培养基为基本培养基,添加不同的植物生长调节物质,进行了离体快速繁殖技术研究。结果表明:采用2.0%NaClO处理20min的灭菌效果好;诱导早花忍冬侧芽分化的适宜培养基为MS+1.0mg/L 6-BA+0.05mg/L NAA+3.0%蔗糖+0.7%琼脂;增殖培养基为MS+2.0mg/L 6-BA+0.05mg/L NAA+3.0%蔗糖+0.7%琼脂,30d的增殖系数为8;最佳的生根培养基为1/2MS+0.3mg/L IBA+0.3mg/L NAA+1.5%蔗糖+0.7%琼脂,生根率达100%。     

Sublytic C5b-9 induces protective autophagy in cultured podocytes

AIM: In podocytes, autophagy occurs at a high basal level and dysregulated autophagy is associa-ted with a variety of podocytopathies. This paper is to investigate the role of autophagy in sublytic C5b-9-induced podocyte injury. METHODS: Sublytic complement C5b-9 stimulation was used as an in vitro model. Autophagosomes were confirmed using monodansylcadaverine (MDC) staining. Immunoblotting was used to measure the change of autophagy-related markers. Cellular morphological changes were observed by Wright-Giemsa staining. Immunofluorescence staining and confocal microscopy were used to detect the expression and distribution of nephrin. The cell viability was assessed by methylthiazol tetrazolium (MTT) assay. The cell apoptosis was assessed by Annexin V-fluorescein isothiocyanate/PI staining. RESULTS: For ensuring sublytic complement injury, the maximal amounts of anti-podocyte antiserum and 160×-diluted normal human serum were used without inducing cell lysis (defined as >5% LDH release). Sublytic C5b-9 promoted autophagy of podocytes in vitro. The proautophagic effect of sublytic C5b-9 manifested in the form of accumulated MDC-labeled vesicles and enhanced the expression of LC3-Ⅱ. Autophagy inhibitor 3-methyladenosine (3-MA) promoted sublytic C5b-9-induced podocyte morphological abnormalities. Compared with the sublytic C5b-9-injured podocytes, 3-MA exposure further decreased the expression of nephrin. 3-MA enhanced sublytic C5b-9-induced podocyte apoptosis. CONCLUSION: Sublytic C5b-9 attack induces autophagy, which may play a protective role against complement-mediated podocyte injury.     

猪轮回杂交的杂种优势之辩证

文章辩证地分析了猪轮回杂交的利与弊。作者指出：畜禽经济杂交在实践中应用的各类方法都有其适用范围和局限性。只要掌握了杂种优势的基本原理以及获得杂种优势的方法,就能在生产实践中扬长避短,将轮回杂交的缺点降到最低,并将其优点发挥到极致。     

木耳菌棒力学性能试验

以经过栽培的木耳菌棒为试验材料,在万能试验机上对不同含水率的菌棒试样进行剪切、压缩的力学性能试验。试验结果表明:含水率是影响菌棒剪切强度、抗压强度的重要自然因素。平均含水率分别为11.75%,21.16%,27.68%的菌棒其平均剪切强度分别为29.4k Pa、20k Pa、14.5k Pa。平均含水率分别为14.3%,24%的菌棒,其平均抗压强度分别为144.7k Pa、77.7k Pa。菌棒的含水率与剪切强度,抗压强度均呈幂指数关系。这些都为研制菌棒粉碎机械、选择粉碎参数提供了设计依据。     

Analysis on Differential Expression Genes in Porcine Aortic Vascular Endothelial Cells Induced by Apolipoprotein C Ⅲ

The aim of this study was to investigate the differential expression genes induced by ApoCⅢ,and study the function of ApoCⅢ.Porcine aortic vascular endothelial cells were successfully isolated using enzyme digestion,and then screened the differential expression genes induced by ApoCⅢ using the Solexa high-throughput sequencing technology.The results showed 647 differential expression genes,including 390 up-regulated genes and 257 down-regulated genes.The qRT-PCR results verified that the gene expression results from Solexa sequencing data were reliable.GO and Pathway analysis showed that the function of differential expression genes were related to immune response,cell apoptosis and death.These findings suggested that ApoCⅢ affected the physiological function of porcine aortic endothelial cells by the molecular pathways of inflammation,cell adhesion and apoptosis,which provided a theoretical basis for further understanding the molecular mechanisms of atherosclerosis caused by ApoCⅢ.     

Tenogenic induction of equine mesenchymal stem cells by means of growth factors and low-level laser technology

Tendons regenerate poorly due to a dense extracellular matrix and low cellularity. Cellular therapies aim to improve tendon repair using mesenchymal stem cells and tenocytes; however, a current limitation is the low proliferative potential of tenocytes in cases of severe trauma. The purpose of this study was to develop a method useful in veterinary medicine to improve the differentiation of Peripheral Blood equine mesenchymal stem cells (PB-MSCs) into tenocytes. PB-MSCs were used to study the effects of the addition of some growth factors (GFs) as TGFβ3 (transforming growth factor), EGF2 (Epidermal growth factor), bFGF2 (Fibroblast growth factor) and IGF-1 (insulin-like growth factor) in presence or without Low Level Laser Technology (LLLT) on the mRNA expression levels of genes important in the tenogenic induction as Early Growth Response Protein-1 (EGR1), Tenascin (TNC) and Decorin (DCN). The singular addition of GFs did not show any influence on the mRNA expression of tenogenic genes whereas the specific combinations that arrested cell proliferation in favour of differentiation were the following: bFGF2 + TGFβ3 and bFGF2 + TGFβ3 + LLLT. Indeed, the supplement of bFGF2 and TGFβ3 significantly upregulated the expression of Early Growth Response Protein-1 and Decorin, while the use of LLLT induced a significant increase of Tenascin C levels. In conclusion, the present study might furnish significant suggestions for developing an efficient approach for tenocyte induction since the external administration of bFGF2 and TGFβ3, along with LLLT, influences the differentiation of PB-MSCs towards the tenogenic fate.     

岩棉育苗块持水量对番茄幼苗生长的影响

设施土壤次生盐渍化和连作障碍日益严重,岩棉无土栽培是解决诸类问题的途径之一。以"双抗1号"番茄品种为试材,在日光温室条件下,研究了岩棉块育苗期间不同相对持水量(30%、40%、50%、60%、70%)对番茄幼苗株高、茎粗、鲜重、干重的影响。结果表明:较高的岩棉块持水量对番茄幼苗株高有明显的促进作用;育苗结束时不同持水量处理对番茄幼苗茎粗的影响差异不显著;较高持水量处理能显著增加番茄幼苗地上部鲜重和干重,能明显促进根系生长,增加岩棉育苗块底面的根系密度。初步建议岩棉块培育番茄苗时宜采用60%~70%相对持水量。     

园林绿地刺吸害虫及其天敌时序动态及发生规律的研究

为有效保护园林绿地中的自然天敌,以园林植物刺吸害虫及其天敌为调查对象,采用黄板诱集法,研究了园林绿地刺吸害虫及其天敌时序动态及发生规律。结果表明:绿地中京枫多态毛蚜(Periphyllus diacerivorus)、栾多态毛蚜(P.koelreuteria)、居松长足大蚜(Cinara pinihabitans)和白皮松长足大蚜(C.bungeanae)的发生期早于桃粉大尾蚜(Hyalopterus arundimis),且5种蚜虫种群均在夏季高温和天敌共同作用下崩溃;瓢虫和寄生蜂类天敌对碧桃(Pruns perscial f.duplex)上桃粉大尾蚜及捕食螨对碧桃上山楂叶螨(Tetranychus viennensis)的跟随效应明显,而天敌对其它4种植物上蚜虫无明显跟随效应;施药虽可暂时降低阔叶植物上3种蚜虫的种群数量,却大量杀伤了害虫的天敌,停药后3种蚜虫仍爆发成灾,同时使6月后碧桃上山楂叶螨种群爆发和5月中旬后油松(Pinus tabuliformis)上针叶小爪螨(Oligonychus ununguis)种群数量较多。绿地中瓢虫、草蛉、食蚜蝇和寄生蜂类天敌的发生最高峰均位于5月下旬至6月下旬,除草蛉外,绿地中的瓢虫、食蚜蝇和寄生蜂类天敌在7—8月种群数量均极低。草蛉喜欢在五角枫(Acer mono)上栖息或搜寻寄主。