Association analysis for detecting significant single nucleotide polymorphisms for phosphorus-deficiency tolerance at the seedling stage in soybean [Glycine max (L) Merr.]

Tolerance to low-phosphorus soil is a desirable trait in soybean cultivars. Previous quantitative trait locus (QTL) studies for phosphorus-deficiency tolerance were mainly derived from bi-parental segregating populations and few reports from natural population. The objective of this study was to detect QTLs that regulate phosphorus-deficiency tolerance in soybean using association mapping approach. Phosphorus-deficiency tolerance was evaluated according to five traits (plant shoot height, shoot dry weight, phosphorus concentration, phosphorus acquisition efficiency and use efficiency) comprising a conditional phenotype at the seedling stage. Association mapping of the conditional phenotype detected 19 SNPs including 13 SNPs that were significantly associated with the five traits across two years. A novel cluster of SNPs, including three SNPs that consistently showed significant effects over two years, that associated with more than one trait was detected on chromosome 3. All favorable alleles, which were determined based on the mean of conditional phenotypic values of each trait over the two years, could be pyramided into one cultivar through parental cross combination. The best three cross combinations were predicted with the aim of simultaneously improving phosphorus acquisition efficiency and use efficiency. These results will provide a thorough understanding of the genetic basis of phosphorus deficiency tolerance in soybean.     

气相色谱-串联质谱法测定柑橘中5种杀螨剂残留

建立了气相色谱-串联质谱法(GC-MS/MS)检测柑橘中5种杀螨剂残留分析方法。样品经乙腈提取、氯化钠盐析后,取有机相用Qu EChERS方法净化,利用GC-MS/MS在多反应监测(MRM)模式下进行测定,外标法定量。5种杀螨剂在0.005~0.5mg/L浓度范围内呈现良好的线性关系,相关系数r^2均>0.996,方法定量限为0.005 mg/kg。在0.005、0.01和0.1mg/kg 3种添加水平下,加标回收率为70.6%~111.3%,相对标准偏差(RSD)为1.07%~14.64%。本方法简便、重现性良好,可用于柑橘类水果样品中杀螨剂的日常检测。     

8个樱花品种播种育苗技术研究

本文介绍了8个樱花品种的种子从储藏、催芽、播种到苗期管理的试验全过程,总结出一种简便易操作的樱花播种育苗技术,可供生产设施比较简陋的地方借鉴和参考。     

困难立地条件下的赣北地区高速公路边坡植物调查及研究

本研究的目的在于调查困难立地条件下的赣北地区10条高速公路边坡植物群落,筛选出边坡乔木层,灌木层、草本层的优势种植物,总结江西省几十年的高速公路边坡植被恢复模式实践经验,将植被恢复情况较好的边坡整理归纳出生态恢复模式,对植被恢复较差的边坡提出针对性和可操作性的优化对策,为后期高速公路边坡植被恢复及养护工作提供参考,以实现江西省高速公路生态效益、社会效益、观赏效益的最大化.     

Functional porous carbons from waste cotton fabrics for dyeing wastewater purification

A new functional porous carbons (PC-WF) is prepared by activation-pyrolysis method use waste cotton fabrics (WF) as an abundant, cheap and available precursor for removal of Brilliant Crocein (BC-GR) and Cationic Red 2GL (CR-GL) from aqueous phase. The PC-WF was characterized by BET, FTIR, SEM, and XRD techniques, the surface area, total pore volume, average pore diameter was found as 1463.5 m² g^-1, 0.783 cm³ g^-1 and 2.14 nm, respectively. The influences on BC-GR and CR-GL adsorption of various experimental factors such as initial concentration and temperature were investigated. Adsorption kinetics was found to be best represented by the pseudo-second order model. The adsorption capacity was 319.8 mg g^-1 for BC-GR and 842.5 mg g^-1 for CR-GL at 30 °C, respectively. The results indicate that for waste cotton fabrics in particular, the practical application of this process to the production of porous carbon would be possible.     

蚕豆抗绿豆象种质资源的鉴定

仓储型害虫绿豆象对蚕豆的安全储藏和品质经常造成重大损失,严重制约着我国蚕豆产业的发展。选育和利用抗虫品种是防治绿豆象最为安全、有效的途径。在室温条件下,对500份蚕豆资源进行连续两年的绿豆象饱和侵染处理,以期筛选出有利用价值的抗绿豆象种质资源,为蚕豆抗虫性种质创新利用提供依据。筛选结果显示,来源于春播区和秋播区的蚕豆资源,每粒蚕豆虫孔数分别为0.92±0.59、1.05±0.42,差异显著(P0.05);粒色为青绿色(绿色、青绿、乳白)和深色(紫色、黑色、褐色等)的蚕豆资源,每粒虫孔数分别为1.30±0.62、0.85±0.43,差异显著(P0.05)。利用Structure软件和Popgen、MEGA软件分别对500份材料的抗虫性进行群体结构分析和聚类分析,可分为5个与地理分布相关的亚群。同时筛选出H0048、H0092、H0094、H001455、H001471、H001787共6份高抗绿豆象的蚕豆资源。