Quantitation of adenine nucleotides in equine colonic mucosal tissue using high performance liquid chromatography.

The objectives were to use high performance liquid chromatography (HPLC) to validate an established method for adenine nucleotide separation in equine colonic mucosal tissue, to determine the inherent variability in the tissue and extraction method, and to determine the stability of ATP, ADP, and AMP in the tissue with time. Equine colonic mucosal tissue obtained from a single horse was immediately submersed in liquid nitrogen, and stored at -70 degrees C. Samples were lyophilized, extracted, and separated by HPLC. The limit of quantitation was 0.05 microg/mL. The coefficient of variation for the instrument was less than 10% for all nucleotides measured. When the tissue was not homogenized prior to sampling, there were significant differences in adenine nucleotide content between samples. However, when the tissue was homogenized prior to analysis, these differences were no longer significant. There was no significant decrease in ATP, ADP, or AMP content over a 54-day analysis period.     

茶树种植密度与排列方式对个体与群体结构的通径分析

本文用通径分析方法对茶树不同种植密度与茶树骨架形成、分枝结构、蓬面结构三大要素进行对应分析,结果表明:1)随密度上升主干粗度、一、二级分枝粗度下降;2)分枝角度随密度上升而变小;3)随密度上升,小桩数随之增加,其它因子与之呈负相关。由于密度上升后,群体结构的光合面积增大,碳水化合物积累增多,而产量上升。选用合理密度与排列组合时,可用回归方程计算。     

非洲黑鸵鸟人工饲养条件下繁殖性能的观察

14只2岁非洲黑鸵鸟按公母比例1:2～3分成4个繁殖组合,对产蛋、受精、孵化情况进行观察分析。结果显示,每只母鸵鸟年平均产蛋量为55.2枚,最高约为73枚,最低为39枚。4个组合鸟群之间种蛋的受精率存在显著的差异P<0.01,I组的受精率最高,为59.6%;IV组的受精率最低,仅8.9%。各组的受精率、孵化率差异不显著P>0.05,平均受精蛋孵化率达到92.6%。本次受精率较低的原因为公鸟年龄小,没有完全达到性成熟。     

Effect of dietary vitamin C and ammonia concentration on the cellular defense response of Macrobrachium nipponense

This study examined the effects of dietary vitamin C and ammonia concentration on the cellular defense response of Macrobrachium nipponese . The response of the cellular defense was determined by monitoring changes in reactive oxygen intermediates in hemocytes, superoxide dismutase activity, catalase activity, glutathione peroxidase activity, glutathione reductase and glutathione transferase activity in muscle. The results showed that prawns fed the basal diet had a significant increase in reactive oxygen intermediates in response to exposure to ammonia-N. When dietary vitamin C was added to the feed, there was a significant decrease in reactive oxygen intermediates in response to exposure to ammonia-N. Total superoxide dismutase and glutathione peroxidase activity of prawns fed the basal diet decreased as ammonia-N increased. This decrease in activity was significantly less pronounced in prawns that were fed the dietary vitamin C supplement. In prawns that were fed the dietary vitamin C supplement there was no significant variation in catalase reductase or gluxathione reductase activity in response to variations in ammonia-N concentration. The glutathione transference activity increased at a low concentration of ammonia-N in prawns that were fed the dietary vitamin C supplement, but there was no significant variation in the prawns fed the basal diet. Overall the results indicate that dietary vitamin C supplements enhance the capacity of M. nipponese to respond to elevations in ammonia-N. This response involved several biochemical reactions known to be involved in cellular defense.     

Particle Size Distribution in Two Lipid Emulsions Used for the Enrichment of Artemia nauplii as a Function of Their Preparation Method and Storage Time

The present study examined the distribution of particle sizes in two experimental standardized lipid emulsions (distributed by ICES, International Council for the Exploration of the Sea) as a function of the preparation method (hand shaking or ultrasonic blending) and as a function of storage time. A 24-h enrichment trial compared the incorporated HUFA levels in the nauplii of Artemia franciscana .
The emulsion droplets in the 50% HUFA emulsion (ICES 50, ethyl ester based) were much smaller than in the 30% HUFA emulsion (ICES 30, triacylglycerol-based) in which 90% of the droplets had a diameter below 12.3 μm as compared to 1.3 pn in ICES 50. The blending method highly affected particle sizes. High-shear blending instead of the classical hand shaking of the emulsion of both ICES 30 and 50 reduced the mean particle size from 5.06 to 1.07 μm and from 0.38 to 0.11 μm, respectively. The particle size distributions remained stable during the 1-wk storage, suggesting the absence of agglomeration or bacterial development. The fatty acid composition of 24-h enriched Artemia reflected differences in the HUFA profile of the emulsions, but was independent of observed differences in the size of the particles.     

离心式压缩机喘振问题分析

当压缩机内气体的流量较低时,气体容易出现旋转脱离现象,导致压缩机不能正常工作,采取增加压缩机内部液体的流量,在出口处设置单向阀防止气体倒流等措施,可有效地防止喘振的发生,保证输气管道的正常运行.     

污水污物潜水旋流泵性能及设计方法试验研究

对影响潜水旋流泵性能的6个主要过流件几何参数进行了试验研究，初步掌握了旋流泵叶轮及泵体主要几何尺寸对性能影响的规律。在分析16种优秀水力模型的基础上，计算出叶轮D2及b2函数回归方程式，并用设计实例进行检验，样机效率比国内外同类产品提高3％左右。     

压力实时监测法在管道泄漏检测中的应用

检测输油管道漏油的方法很多，介绍了通过压力实时监测确定输油管道漏油点方法，以及实际应用情况。实践证明，通过压力实时监视确定输油管道漏油点的方法在实际输油运行中是切实可行的。     

寿光蔬菜叫响"文化品牌"

王高银刹、仓颉双井、圣城古槐等寿光古代及现代的知名景观,全部“搬进”了第六届菜博会的土展厅,不过组成景点的不是土木砖瓦,而是清一色的蔬菜瓜果。这些普通蔬菜瓜果的独到组合,不仅组成了令人留连的“蔬菜大观园”,而且展现出“菜乡”寿光传统的民俗文化和蔬菜产业深厚的文化底蕴。寿光蔬菜种植历史悠久,北魏农学家贾思勰就诞生在这里,明清年间,“独根红”韭菜被确定为宫廷贡品。为叫响蔬菜的“文化品牌”,