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41.
42.
The putative neurotransmitters, glycine and serine, significantly enhanced the sleeping time (loss of the righting reflex) that was induced by ethanol in mice. The observed synergistic effect between ethanol and the amino acids is probably not related to an alteration of ethanol metabolism, but rather to an interaction of these compounds in the central nervous system. 相似文献
43.
J C Wallace G Galili E E Kawata R E Cuellar M A Shotwell B A Larkins 《Science (New York, N.Y.)》1988,240(4852):662-664
Zeins, the storage proteins of maize, are totally lacking in the essential amino acids lysine and tryptophan. Lysine codons and lysine- and tryptophan-encoding oligonucleotides were introduced at several positions into a 19-kilodalton zein complementary DNA by oligonucleotide-mediated mutagenesis. A 450-base pair open reading frame from a simian virus 40 (SV40) coat protein was also engineered into the zein coding region. Messenger RNAs for the modified zeins were synthesized in vitro with an SP6 RNA polymerase system and injected into Xenopus laevis oocytes. The modifications did not affect the translation, signal peptide cleavage, or stability of the zeins. The ability of the modified zeins to assemble into structures similar to maize protein bodies was assayed by two criteria: assembly into membrane-bound vesicles resistant to exogenously added protease, and ability to self-aggregate into dense structures. All of the modified zeins were membrane-bound; only the one containing a 17-kilodalton SV40 protein fragment was unable to aggregate. These findings suggest that it may be possible to create high-lysine corn by genetic engineering. 相似文献
44.
Mitochondrial DNA mutation associated with Leber's hereditary optic neuropathy 总被引:173,自引:0,他引:173
D C Wallace G Singh M T Lott J A Hodge T G Schurr A M Lezza L J Elsas E K Nikoskelainen 《Science (New York, N.Y.)》1988,242(4884):1427-1430
Leber's hereditary optic neuropathy is a maternally inherited disease resulting in optic nerve degeneration and cardiac dysrhythmia. A mitochondrial DNA replacement mutation was identified that correlated with this disease in multiple families. This mutation converted a highly conserved arginine to a histidine at codon 340 in the NADH dehydrogenase subunit 4 gene and eliminated an Sfa NI site, thus providing a simple diagnostic test. This finding demonstrated that a nucleotide change in a mitochondrial DNA energy production gene can result in a neurological disease. 相似文献
45.
Administration of estradiol 17beta to male Xenopus laevis induces the appearance in serum of large amounts of a serum lipophosphoprotein which is not readily removed from the circulation and which can be resolved from other serum components by chromatography on triethylaminoethyl-cellulose. The initial rate of production of serum lipophosphoprotein is independent of the amount of estrogen administered, but the maximum rate of production and the time taken to attain this rate are dose-dependent. 相似文献
46.
Alward WL 《Science (New York, N.Y.)》2003,299(5612):1527-1528
47.
48.
Active Cavity Radiometer Irradiance Monitor (ACRIM) solar constant measurements from 1980 to 1986 are compared with ground-based, irradiance spectrophotometry of selected Fraunhofer lines. Both data sets were identically sampled and smoothed with an 85-day running mean, and the ACRIM total solar irradiance (S) values were corrected for sunspot blocking (S(c)). The strength of the mid-photospheric manganese 539.4-nanometer line tracks almost perfectly with ACRIM S(e), Other spectral features formed high in the photosphere and chromosphere also track well. These comparisons independently confirm the variability in the ACRIM S(e), signal, indicate that the source of irradiance is faculae, and indicate that ACRIM S(e), follows the 11-year activity cycle. 相似文献
49.
Wallace P 《Science (New York, N.Y.)》1974,185(4156):1035-1037
50.