EZH2-mediated regulation of NF-κB target gene expression in gastric cancer

AIM: To explore the mechanism by which over-expression of enhancer of zeste homolog 2 (EZH2) in a panel of gastric cancer cell lines is involved in tumorigenesis of gastric cancer. METHODS: Real-time PCR and Western blot were employed to examine the mRNA and protein levels of EZH2, respectively. MTS assay, cell migration and soft agar assay were performed to investigate the role of EZH2 in the regulation of stomach cancer behaviors. The effect of EZH2 on NF-κB target gene expression was determined by Luciferase reporter and real-time PCR. Co-immunoprecipitation was used to analyze the interaction of EZH2 and p65 in HEK293T cells. RESULTS: The expression levels of EZH2 were significantly increased in the gastric cancer cells compared with normal gastric epithelial cells. Pharmacological inhibition by DZNep or knockdown of EZH2 significantly compromised AGS and SNU-16 cell activity, cell migration and anchorage-independent cell growth. Moreover, siRNA knockdown of EZH2 impaired NF-κB downstream targets, such as IL-8, CXCL5 and CCL20. In addition, the interaction of EZH2 and p65 was detected. CONCLUSION: EZH2 mediates the growth of gastric cancer cells through the regulation of NF-κB downstream gene expression.     

广西县级林地变更调查试点技术方法

为探索广西县级林地年度变更调查技术方法,以鹿寨县为例,运用遥感自动检测技术、森林资源管理信息平台等,对地类及管理属性变化图斑进行变更,形成本期林地数据库和林地变化数据库。文章分析了林地变更调查的关键技术方法,指出了存在的问题,并提出相应的建议。     

美洲黑杨无性系苗期光合生理特性研究

研究了9个美洲黑杨无性系的光合生理特性,为优良新无性系的选育提供参考。采用LI-6400便携式光合作用测定系统测定美洲黑杨的光合作用日变化及其光响应曲线,利用非直角双曲线Farquhar模型、二次曲线及直线方式拟合光响应曲线,分析比较光响应特征参数及影响净光合速率因子。结果表明:各无性系的净光合速率均具有明显的日变化规律,tn01-78、tn01-38、tn04-n52呈双峰曲线现象,2个峰值出现的时间在11:00和14:00左右,其余呈单峰曲线,净光合速率最大值出现在12:00左右;不同无性系最大净光合速率、暗呼吸速率、表观量子效率、光饱和点和光补偿点的大小范围分别为20.50~31.42μmol·m-2s-1、1.67~3.19μmol·m-2s-1、0.055~0.279μmol·μmol-1、950.83~1 360.83μmol·m-2s-1和29.03~55.20μmol·m-2s-1;净光合速率(Pn)变化与气孔导度Gs、水压亏缺Vpdl、空气温度Tair和光合有效辐射VPAR呈正相关,与胞间CO2浓度Ci和大气相对湿度(HR)呈负相关;净光合速率与影响因子的相关程度依次为气孔导度>光合有效辐射>胞间CO2浓度>大气相对湿度>空气温度>水压亏缺。     

Impact of fluxapyroxad on the microbial community structure and functional diversity in the silty-loam soil

The aim of this work was to assess the effect of applying three different doses of fluxapyroxad on microbial activity, community structure and functional diversity as measured by respiration, microbial biomass C, phospholipid fatty acid(PLFA) and community-level physiological profiles(CLPPs). Our results demonstrated that substrate-induced respiration(on day 15) and microbial biomass C(on days 7 and 15) were inhibited by fluxapyroxad, but stimulation was observed thereafter. In contrast, fluxapyroxad addition increased the basal respiration and metabolic quotients(q CO2) and respiratory quotients(QR). Analysis of the PLFA profiles revealed that the total and bacterial biomass(both Gram-positive bacteria(GP) and Gram-negative bacteria(GN)) were decreased within the initial 15 days, whereas those as well as the GN/GP ratio were increased at days 30 and 60. F luxapyroxad input decreased the fungi biomass but increased the bacteria/fungi ratio at all incubation time. Moreover, high fluxapyroxad input(75 mg fluxapyroxad kg–1 soil dry weight) increased the microbial stress level. A principal component analysis(PCA) of the PLFAs revealed that fluxapyroxad treatment s ignificantly shifted the microbial community structure, but all of the observed effects were transient. Biolog results showed that average well color development(AWCD) and functional diversity index(H′) were increased only on day 60. I n addition, the dissipation of fluxapyroxad was slow in soil, and the degradation half-lives varied from 158 to 385 days depending on the concentration tested.     

红甜菜的特殊功效及应用

综述了红甜菜作为园林美化用途之外的其它特殊用途,包括营养保健、治疗及预防肿瘤、治疗高血压、糖尿病、降低血脂、增强免疫力、抗疲劳、糖甜菜辅助育种以及作为纺织业染色新材料等方面的特殊用途。为将来红甜菜的深入开发提供参考。     

白羊草R2R3-MYB转录因子的挖掘及对干旱胁迫反应的研究

本文以白羊草（Bothriochloa ischaemum）为材料，利用其目前已有的转录组序列，通过搜索、比对、功能域多态性分析、进化树构建和基因表达分析等手段，对白羊草R2R3-MYB型转录因子进行挖掘和干旱胁迫下的表达模式分析。结果从白羊草挖掘出43个R2R3-MYB转录因子，它们具有高度保守的[W]-X（19）-[W]-X（19）-[W]-X（n）-[W]-X（18）-[W]结构，主要参与生长发育、次生代谢和逆境响应等生物过程。通过进一步的分析，本研究筛选出8个可能参与干旱胁迫响应的R2R3-MYB转录因子。这为白羊草R2R3-MYB基因的功能研究和开发利用奠定了基础。     

中国信用风险缓释工具创新试点最新进展研究

信用风险缓释工具是中国银行间市场2010年创新试点推出的信用风险管理工具，它将短期融资券、中期票据和贷款等信用产品的信用风险剥离定价，并转移给愿意承担风险的投资者，其推出从根本上改变了商业银行等金融机构信用风险管理的传统特征。通过对信用风险缓释工具试点中投资主体培育、市场定价、做市商机制、信息披露、市场外部环境建设等问题进行研究，有利于信用缓释工具功能的充分发挥，有利于完善债券市场信用风险分担机制、有利于商业银行等投资者动态、专业地管理信用风险。     

基于加权Voronoi图的林木竞争指数

以福寿山林场25年生的杉木生态公益林为研究对象,通过分析林木竞争指数与胸径生长因子的相关关系,得出二者为负相关,从而确定加权Voronoi图的权重为胸径倒数(W),借助Arcgis软件里的加权Voronoi图工具,通过加权Voronoi图的方法来确定竞争单元,提出基于加权Voronoi图的W_V_Hegyi竞争指数,最后将W_V_Hegyi竞争指数与Hegyi、V_Hegyi竞争指数进行对比分析,结果表明:1) 3种竞争指数与胸径相关性强弱排序为:W_V_HegyiV_HegyiHegyi;2) 3种竞争指数与胸径服从幂函数关系,曲线拟合度排序为:W_V_HegyiV_HegyiHegyi;3) 样地竞争指数平均值排序均为:V_HegyiW_V_HegyiHegyi。通过以上比较分析证明,W_V_Hegyi竞争指数比Hegyi、V_Hegyi竞争指数能更准确地反映林木间的竞争关系。      

国家经济安全审计实施路径研究

国家经济安全审计是指审计机关依赖有效的审计制度对被审单位财政、财务收支和经济活动的真实性、合法性和效益性进行审计的基础上，针对经济安全的关键领域，识别、评估、预警和报告危害经济安全的风险，确保财产权益安全完整的一种审计模式。该模式奠定了审计监督在国家经济安全体系中的基础性地位。创新审计模式及审计策略、变革国家审计体制、构建以审计监督为基础的新型国家经济安全体系是国家经济安全审计的三大实施路径。     

无缝烟夹不同针尖处理对烤后烟叶孔径的影响

为优化无缝烟夹装置设计,充分发挥无缝烟夹装烟方式的优势,对不同直径、不同形状针尖及不同方向的无缝烟夹烤后烟叶的针孔面积进行了比较研究。结果表明:不同直径烟针相比,3 mm直径无缝烟夹烤后针孔面积小于5 mm直径无缝烟夹;无缝烟夹在3 mm直径条件下,扁形针尖平行处理烤后针孔面积最小;在5 mm直径条件下,圆形针尖平行处理烤后针孔面积最小。