核桃芽接育苗技术

研究分析了核桃播种方式，播种方法，播种量，接穗培养,家接时间，剪砧程度，嫁接方法等芽接育苗技术的关键，使田间大面积嫁接成活率可达93％左右。     

饥饿与再投喂对方斑东风螺生长、基本营养成分及RNA/DNA比值的影响

在(25.8±1.7)℃条件下,测定了方斑东风螺(5.25±0.53)g在不同时间(7、15、25和40d)饥饿处理后再投饵30d过程中的生长参数、基本营养成分及组织RNA/DNA比值的变化。饥饿状态下,螺体水分含量逐渐上升,第15天时显著高于对照组;脂肪与糖原含量均下降,并分别于饥饿15d、25d时显著低于对照组(P<0.05);而蛋白质含量在不同处理组间无显著性差异(P>0.05);足肌与肝胰脏中RNA/DNA比值均随饥饿时间延长而逐渐降低。恢复生长后,除饥饿40d组含水量显著高于对照组外(P<0.05),该组其余营养成分及其它各组相应指标均恢复至或接近对照组;RNA/DNA比值除在饥饿40d组肝胰脏中仍较低外均接近或显著高于对照组。各组幼螺摄食率(FR)均高于或显著高于对照组(P<0.05),体重增量、食物转化率(FCE)在前3处理组及特殊生长率(SGR)在饥饿7d与25d组均与对照组间无显著差异(P>0.05),而饥饿15d组的SGR显著高于对照组(P<0.05);饥饿40d组尽管FR显著提高,但体重增量、FCE及SGR均显著低于对照组(P<0.05)。结果表明,方斑东风螺幼螺饥饿时主要消耗脂肪与糖原...     

层次分次法在人工植被选择上的应用

将人工植被生长所需的条件用层次分析法进行比较和判断,最后做出选择种植何种植物的决策.运用层次分析法针对温度、水分和土壤3个要素建立层次结构模型,构造各项评价指标的判断矩阵,并计算权重.通过定性分析与定量分析相结合的方式来确定待种植物,可以实现植被种植的科学性和合理性.     

秸秆膨化机度电产量的生产参数优化及影响因素分析

本文对影响秸秆膨化机度电产量的主要生产参数—秸秆含水率(X1)、秸秆粒度(X2)和螺杆转速(X3)进行了研究,揭示了度电产量与这些参数之间的内在规律,创建了秸秆度电产量与三个参数之间的数学模型,进行了生产参数优化,并就生产参数对度电产量的影响进行了分析。     

澄海莱芜人工鱼礁集鱼效果初步评价

2003和2007年分别对澄海莱芜人工鱼礁区进行了投礁前的本底调查和投礁后的跟踪调查。结果表明,投礁后礁区海域游泳生物的资源密度明显比投礁前高,增加了25．63倍;礁区海域各类资源种类均比投礁前丰富,总种数由投礁前的23种增加至41种,比投礁前增加了0．78倍,其中,蟹类种数增加最多,增加了1．75倍;在本底调查中没有出现的经济种类龙头鱼Harpodon nehereus和红星梭子蟹Portunus sanguinolentus在跟踪调查中已成为主要优势种;Shannon—Wienver多样性指数（H’）在礁区和对比区均比投礁前有所增加。表明鱼礁投放后,鱼礁区集鱼效果和群落结构明显改善,人工鱼礁建设取得了明显的生态效益和经济效益。     

原料表面碱液处理对稻草碎料板力学性能的影响

在实验室条件下探索了稻草原料表面碱液处理对稻草碎料板力学性能的影响.试验表明,处理后的稻草碎料板的内结合强度比处理前有大幅度提高,弯曲弹性模量和静曲强度比处理前则有所下降,但都达到了相关的国家标准;2h吸水厚度膨胀率升高,超过国家标准.因此稻草表面碱液处理提高了稻草原料表面润湿性和胶合强度,但对稻草碎料板的力学性能有影响.     

松茸菌株对6种松树幼苗的感染及其菌根解剖学研究

利用我国滇产松茸9924菌株及日产松茸99606菌株，分别对云南松、华山松、马尾松、火炬松、湿地松及加勒比松6种松树幼苗实施人工菌根接种。研究结果表明，接种后90d的云南松和湿地松分别有75％和78．1％的幼苗受感染并形成菌根；接种后180d,6种松树幼苗分部都可形成菌根，菌根感染率分别达90．63％－100％，感染指数达50．00－84．68；菌根形态主要有棒状、二叉分枝状及多叉分枝状3种；随树种的不同，各类菌根形态所占比例也不同；不同树种根系上形成菌根的长度也有差异，以马尾松根系上的菌根最长，华山松的菌根最短；此外，各种组合之间在菌套厚度，哈蒂氏网结构上也略有差别。     

Role of PPARα/PGC-1α in doxorubicin induced mouse dilated cardio-myopathy

AIM: To investigate the changes of peroxisome proliferator-activated receptors (PPAR)α/peroxisome proliferator activated receptor coactivator 1 alpha (PGC-1α) in doxorubicin (DOX) induced dilated cardiomyopathy (DCM) and its effect on the energy metabolism and myocardial function in mice. METHODS: Forty mice were randomly divided into 4 groups: control group, DOX group, PPARα inhibitor group and PPARα agonist group. The DCM model was established by injection of DOX. The protein levels of PPARα/PGC-1α were detected. The PPARα inhibitor and PPARα agonist were used 2 weeks beforeinjection of DOX. The contents of adenine acid and phosphocreatine (Pcr) in the mitochondria were measured by high-performance liquid chromatography (HPLC). The ANT activity was analyzed by the atractyloside-inhibitor stop technique. The changes of the echocardiography and hemodynamics were also observed. RESULTS: DOX induced DCM model was successfully established. The protein levels of PPARα and PGC-1α in control group were significantly higher than those in DOX group (P<0.05). Both of the high-energy phosphate contents and the transport activity of ANT were decreased in DOX group (P<0.05), and the hemodynamic parameters were disordered (P<0.01). Compared with DOX group, PPARα inhibitor pre-treatment significantly reduced the PPARα/PGC-1α expression. Meanwhile, high-energy phosphate contents in the mitochondria and the ANT transport activity of the mitochondria decreased, as well as the left ventricular function (P<0.05). On the other hand, PPARα agonist significantly increased the expression of PPARα and PGC-1α, and improved the transport activity of ANT. In addition, the hemodynamic parameters were ameliorated, but the high-energy phosphate contents of the mitochondria did not significantly change. CONCLUSION: PPARα/PGC-1α plays an important role in the regulation of ANT transport activity in dilated cardiomyopathy induced by DOX, and the activation of PPARα/PGC-1α has protective effects on the DCM induced by DOX.     

Juglone inhibits epithelial-mesenchymal transition of prostate cancer cells by regulating Wnt/β-catenin/Snail signaling pathway

AIM: To investigate the mechanism of juglone on epithelial-mesenchymal transition in prostate cancer cells. METHODS: Human prostate cancer LNCaP cells were divided into control group (without juglone), 12.5 μmol/L juglone group and 25 μmol/L juglone group. LNCaP cells in the latter 2 groups were treated with juglone for 24 h. The invasion ability of the LNCaP cells was detected by Transwell assay. The protein expression of E-cadherin, vimentin, Snail and β-catenin was determined by Western blot. The LNCaP cells were treated with LiCl and juglone in combination for 24 h, and the protein expression of Snail and E-cadherin was detected by Western blot.RESULTS: The results of Trans-well invasion assay showed that the invasion ability in juglone groups was significantly decreased (P<0.01). The protein expression of E-cadherin in the LNCaP cells treated with juglone was increased, and the expression levels of vimentin and β-catenin were reduced (P<0.01). Treatment with LiCl significantly attenuated the inhibitory effect of juglone on Snail expression and subsequent down-regulation of E-cadherin expression. CONCLUSION: Juglone inhibits the epithelial-mesenchymal transition by inhibiting the Wnt/β-catenin/Snail signaling pathway in the LNCaP cells.     

杨树大苗造林地膜覆盖技术

结合常规造林方法应用地膜覆盖技术,提高了杨树大苗造林成活率,加快了工程造林步伐,解决了东北地区春季大风、干旱、无雨的干燥恶劣天气条件下进行大规模工程造林成活率低的问题,达到一季造林、一次成活、一次成林。同时节省了造林资金。