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131.
Locomotor primitives in newborn babies and their development 总被引:1,自引:0,他引:1
Dominici N Ivanenko YP Cappellini G d'Avella A Mondì V Cicchese M Fabiano A Silei T Di Paolo A Giannini C Poppele RE Lacquaniti F 《Science (New York, N.Y.)》2011,334(6058):997-999
How rudimentary movements evolve into sophisticated ones during development remains unclear. It is often assumed that the primitive patterns of neural control are suppressed during development, replaced by entirely new patterns. Here we identified the basic patterns of lumbosacral motoneuron activity from multimuscle recordings in stepping neonates, toddlers, preschoolers, and adults. Surprisingly, we found that the two basic patterns of stepping neonates are retained through development, augmented by two new patterns first revealed in toddlers. Markedly similar patterns were observed also in the rat, cat, macaque, and guineafowl, consistent with the hypothesis that, despite substantial phylogenetic distances and morphological differences, locomotion in several animal species is built starting from common primitives, perhaps related to a common ancestral neural network. 相似文献
132.
Identification of vaccine candidates against serogroup B meningococcus by whole-genome sequencing 总被引:1,自引:0,他引:1
Pizza M Scarlato V Masignani V Giuliani MM Aricò B Comanducci M Jennings GT Baldi L Bartolini E Capecchi B Galeotti CL Luzzi E Manetti R Marchetti E Mora M Nuti S Ratti G Santini L Savino S Scarselli M Storni E Zuo P Broeker M Hundt E Knapp B Blair E Mason T Tettelin H Hood DW Jeffries AC Saunders NJ Granoff DM Venter JC Moxon ER Grandi G Rappuoli R 《Science (New York, N.Y.)》2000,287(5459):1816-1820
Neisseria meningitidis is a major cause of bacterial septicemia and meningitis. Sequence variation of surface-exposed proteins and cross-reactivity of the serogroup B capsular polysaccharide with human tissues have hampered efforts to develop a successful vaccine. To overcome these obstacles, the entire genome sequence of a virulent serogroup B strain (MC58) was used to identify vaccine candidates. A total of 350 candidate antigens were expressed in Escherichia coli, purified, and used to immunize mice. The sera allowed the identification of proteins that are surface exposed, that are conserved in sequence across a range of strains, and that induce a bactericidal antibody response, a property known to correlate with vaccine efficacy in humans. 相似文献
133.
Martella V Elia G Decaro N Di Trani L Lorusso E Campolo M Desario C Parisi A Cavaliere N Buonavoglia C 《Veterinary microbiology》2007,121(1-2):56-63
In December 2005, equine influenza virus infection was confirmed as the cause of clinical respiratory disease in vaccinated horses in Apulia, Italy. The infected horses had been vaccinated with a vaccine that contained strains representatives from both the European (A/eq/Suffolk/89) and American (A/eq/Newmarket/1/93) H3N8 influenza virus lineages, and the H7N7 strain A/eq/Praga/56. Genetic characterization of the hemagglutinin (HA) and neuraminidase (NA) genes of the virus from the outbreak, indicated that the isolate (A/eq/Bari/2005) was an H3N8 strain closely related to recent representatives (Kentucky/5/02-like) of the American sub-lineage Florida, that was introduced in Italy through movement of infected horses from a large outbreak described in 2003 in United Kingdom. Strain A/eq/Bari/2005 displayed 9 amino acid changes in the HA1 subunit protein with respect to the reference American strain A/eq/Newmarket/1/93 contained in the vaccine. Four changes were localized in the antigenic regions C-D and likely accounted for the vaccine failure. 相似文献
134.
Decaro N Campolo M Desario C Ricci D Camero M Lorusso E Elia G Lavazza A Martella V Buonavoglia C 《Veterinary microbiology》2005,109(1-2):19-27
A mammalian orthoreovirus (MRV) strain was isolated from a pup with fatal diarrhea, which had a concurrent infection by canine parvovirus type 2. The reovirus isolate showed an atypical hemagglutination pattern and a retarded electrophoretic mobility of the S1 segment, which is characteristic of MRV type 3 (MRV-3). Assignment of the isolated virus to MRV-3 was confirmed by type-specific RT-PCR assays, targeting the S1 gene, and by subsequent sequence analysis of the PCR product. By phylogeny based on the S1 gene of several MRVs, the isolate fell into lineage E, along with the murine strain T3C9/61 and the bovine strains T3C18/61 and T3C31/59. Conversely, L1 sequences were found to segregate regardless of the viral type. A total of 110 fecal samples, 56 nasal and 31 ocular swabs from dogs with diarrhea or nasal/ocular discharge were tested by a nested-PCR assay specific for reoviruses, and no sample was found to contain MRV RNA, a finding that is apparently in contrast with the seroprevalence (25.77%) observed in dogs. 相似文献
135.