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21.
Toru TANIUCHI Takayuki KANAYA Shuuichi UWABE Takahito KOJIMA Seiji AKIMOTO Isamu MITANI 《Fisheries Science》2004,70(5):845-851
ABSTRACT: Age and growth of alfonsino Beryx splendens (Lowe) were studied using counts of presumed daily incremental growth rings on the transverse section of otoliths of fish collected from the Kanto District, central Japan. Microstructural growth increments were observed from the core to the outermost margin of the broadest of approximately 50 branches formed on the surface of an otolith. Of 98 otoliths examined, 46 were readable. The number of increments and fish lengths ranged from 448 (218 mm fork length [FL]) to 3701 (411 mm FL). The ages of these fish were estimated to be 1 year, 2 months and 10 years, 2 months, respectively, assuming that an increment was formed daily. The von Bertalanffy growth equation combined for males and females was expressed as follows: L t = 542 {1 − exp [−0.133( t + 2.00)]}, where L t is fork length (mm) at yearly age t . The results of the present study were compared with those of other researchers who had studied alfonsino from central Japan. 相似文献
22.
Osamu Yamato Eun-Og Jo Toru Shoda Masahiro Yamasaki Yoshimitsu Maede 《Journal of veterinary diagnostic investigation》2004,16(5):469-472
This report describes a rapid and simple method for mutation screening of G(M1) gangliosidosis in Shiba dogs by direct amplification of DNA from canine whole-blood specimens using a novel polymerase chain reaction (PCR) reagent cocktail, which can eliminate the DNA extraction process and amplify the genomic DNA directly from human or murine whole blood. The strategy of this mutation screening is based on the identification of a nucleotide deletion by restriction enzyme analysis, coupled with the direct PCR amplification. The target sequence of the canine beta-galactosidase gene could be amplified directly from various forms of canine whole-blood specimens, including anticoagulated blood, blood stored frozen for 1 year, dried blood held in filter paper for 1 year at room temperature, and dry powder of blood stripped from Giemsa-stained blood films, which had been prepared 10 years earlier, resulting in the determination of genotypes in all the specimens. This method simplified the molecular diagnosis and carrier screening of G(M1) gangliosidosis in Shiba dogs, making it simple to examine specimens from the large, widely distributed population of these dogs. 相似文献
23.
Toru Kohama Nobuya Mizoue Satoshi Ito Akio Inoue Kotaro Sakuta Hiroyuki Okada 《Journal of Forest Research》2006,11(4):235-242
We examined the extent to which direct and indirect measures of light and microsite conditions could explain variation in
tree height and diameter at the base of 6-year-old Cryptomeria japonica trees planted in a group selection opening of about 0.32 ha on a steep slope at Shiiba, Miyazaki Prefecture, southern Japan.
We first used the gap light index (GLI) and soil thickness (ST) as directly measured indices. For an indirect measure of light, we used a between-cohort competition index (BCI) estimated from the position and total height of residual trees. For indirect measures of microsite, we examined topographic
indices (slope, plan and profile curvature, average slope gradient, and relative elevation) derived from digital elevation
models (DEMs) with different resolutions ranging from 2 to 10 m. The multiple linear regression using GLI and ST explained about 45% of variation in tree size, while simple regression using only GLI explained about 35%. The contribution of ST was about half of GLI. The multiple regressions using BCI and the topographic indices did not explain any more variation than using BCI alone (R
2 of about 0.26). We conclude that microsite conditions with shallower soil and steeper slope have negative effects on tree
growth in group selection openings, although the relative importance is smaller than light conditions. More comprehensive
studies considering several openings with more heterogeneous topography including different species are needed to generalize
our growth prediction using the indirect measures, which are useful for practical forest management. 相似文献
24.
Mariko TAKAYASU Kouko HAMAMOTO Hiroshi SATOH Toshihiro ICHIJO Toru TAKAHASHI Kazuhisa FURUHAMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(2):261-263
To clarify the effect of renal dysfunction on pharmacokinetics of the prokinetic agent metoclopramide (MCP), we administered intravenously 0.4 mg/kg MCP to healthy calves and calves subjected to right kidney vessel ligation (ligation) without or with a subsequent left nephrectomy (ligation plus removal). Plasma MCP concentration, glomerular filtration rate (GFR) and plasma prolactin level were measured by liquid chromatography-tandem mass spectrometry, simplified equation using iodixanol and enzyme-linked immunosorbent assay, respectively. Only in calves with ligation plus removal, plasma MCP concentrations were increased significantly 6, 8 and 12 hr after injection, showing that a negative correlation was observed between the plasma MCP concentrations and GFR value. A tendency to increase in plasma PRL concentration was noted also in these calves. In conclusions, plasma MCP concentrations depend on the GFR mode in calves, and its critical GFR value was estimated. 相似文献
25.
Misa HOSOE Nao YOSHIDA Yutaka HASHIYADA Hidetoshi TERAMOTO Toru TAKAHASHI Sueo NIIMURA 《The Journal of reproduction and development》2014,60(4):268-273
Fetal bovine serum (FBS) has been widely used as a supplement in the maturation medium of bovine oocytes in
vitro. However, serum contains many undefined factors and is potentially infectious to humans and animals. As a serum
replacement, we evaluated the feasibility of using the silk protein, sericin, derived from the cocoons of silkworm. To examine the
rates of oocyte maturation and fertilization, cumulus-oocyte complexes were cultured in TCM-199 supplemented with 0.01%, 0.05%,
0.1% or 0.15% sericin or 5% FBS. The sizes of the perivitelline space that might relate to polyspermy, the expressions of
Has2 and CD44 mRNA, the amount of hyaluronan (hyaluronic acid: HA) contained in the oocytes
and the rates of blastocyst formation following insemination were then compared between the oocytes cultured with 0.05% sericin
and 5% FBS, because the polyspermy rates in oocytes cultured with 0.05% sericin were significantly lower than in those cultured
with 5% FBS. After in vitro maturation (IVM), the mean size of the perivitelline space was significantly greater
in oocytes cultured with sericin than in those cultured with FBS, although the rates of nuclear maturation, fertilization and
blastocyst formation of oocytes under both IVM conditions were not significantly different. The expression of HAS2 and
CD44 mRNA and the amount of HA in the denuded oocytes cultured with 0.05% sericin were significantly greater than in
those cultured with FBS. These results indicate the feasibility of sericin as an alternative protein supplement for IVM in bovine
oocytes. 相似文献
26.
Noriyuki Takai Noriyuki Hirose Takuya Osawa Kazuyuki Hagiwara Takahito Kojima Yuji Okazaki Tomohiro Kuwae Toru Taniuchi Kiyoshi Yoshihara 《Fisheries Science》2007,73(3):593-608
ABSTRACT: It is important to clarify trophic dynamics in marine ecosystems for management of the fishing ground. Organic carbon sources and trophic position of pelagic fishes in the coastal waters of the south-eastern Izu Peninsula, Japan, were examined on the basis of carbon and nitrogen stable isotope distributions. The δ13 C of the fishes was mostly distributed from −19 to −16‰ for nektonic fishes (13 species of adults and immatures) and planktonic fishes (10 species of larvae and juveniles), close to the δ13 C values of particulate organic matter and planktonic decapods. These δ13 C signatures for the inhabitants of the water column were in contrast with the high δ13 C values (mainly −16 to −13‰) for demersal fishes of Scorpaeniformes and benthic polychaetes collected in the surf zone. These results indicate that nektonic and planktonic fishes depend on phytoplankton for carbon supply. The δ15 N signatures suggest that the trophic position ranged 3.1–4.5 for the nektonic fishes and 2.9–3.7 for the planktonic fishes, premised on trophic level 3 for larval Japanese anchovy Engraulis japonicus . Thus, planktivorous fishes should be mainly assigned to trophic levels 3 and 4 in this area. 相似文献
27.
Ganzaya Perenlei Hitomi Tojo Toru Okada Masatoshi Kubota Motoni Kadowaki Shinobu Fujimura 《Animal Science Journal》2014,85(10):895-903
We evaluated effects of dietary supplementation with astaxanthin (Ax)‐rich yeast, Phaffia rhodozyma (Xanthophyllomyces dendrorhous), on broiler chicken meat quality. Fourteen‐day‐old female Ross broilers were divided into three groups: control group, Ax‐free diet; Ax 10 group, 10 mg/kg Ax diet; and Ax 20 group, 20 mg/kg Ax diet for 28 days. At 42 days old, chickens were slaughtered, and then growth performance, meat quality and sensory attributes were analyzed. Compared with the control, a* values increased significantly after slaughter and 48 h postmortem for Ax 20 samples (P < 0.05) and for b* values in Ax 20 and Ax 10 groups (P < 0.05). Cooking loss decreased in the Ax 20 group (P < 0.05). After 120 h aging, contents of several free amino acids and total free amino acid content of Ax 20 group were significantly higher than the control (P < 0.05). In sensory evaluation, meat texture attributes improved significantly in the Ax 20 group (P < 0.01). No significant changes occurred in flavor attribute scores of meat soup from the Ax 20 group compared with the control even though most assessors preferred meat soup from the Ax 20 group. Overall, Ax‐rich yeast in the diet improves broiler chicken meat quality. 相似文献
28.
29.
Toshio Shibuya Toru Taniguchi Shuhei Tsukuda Shuji Shiozaki Kaori Itagaki 《New Forests》2014,45(4):589-602
To improve the propagation of Japanese cedar (Cryptomeria japonica D. Don), we investigated the effects of apical and basal temperatures during a water soaking treatment on the adventitious root formation of 70-mm long shoot tip cuttings which have an apical bud. The basal portion of the cuttings was soaked for 28 days in water with temperatures ranging from 10 to 35 °C, at an air temperature of 5 or 10 °C. Control cuttings were soaked in water at 25 °C, with an air temperature of 25 °C. Treated cuttings were then planted in vermiculite rooting medium and grown at an air temperature of 25 °C for 35 days. Adventitious roots initiated earlier and developed more in the cuttings treated with apical temperatures of 5 or 10 °C and basal temperatures of 20–30 °C than in the control cuttings. The rooting percentage was greatest (93 %) in the cuttings treated with a 10/25 °C apical/basal temperature, whereas few control cuttings rooted (13 %). This suggests that the temperature gradient created by warming the basal portion of the cuttings while cooling their apical end stimulates adventitious root formation. When we tested seasonal variation of rootability at 10/25 °C, the rooting percentage increased from early autumn to winter, and decreased from winter to summer. The soluble sugar contents did not directly affect the formation of adventitious roots in the present study. 相似文献
30.