The functioning and sustainability of village crop protection brigades in Niger

In 1992 and 1993, two studies were executed in Niger to evaluate the functioning and sustainability of village plant protection brigades. The history of these brigades, their functioning, and the way the training was put into practice are discussed, as well as the problems encountered by the brigadiers. Also, the division of benefits of the brigade within the villages is dealt with. The approach adopted by the programme is discussed. It is argued that It is unlikely that many brigades will continue to exist after the end of the programme, given the costs and availability of pesticides and equipment. The fact that the bigger and richer farmers within the village benefit most undermines community based‐support. The roots of these problems can be traced back to the top‐down approach. A more participatory approach, based on the enhancement of farmers’ decision‐making and their knowledge of the environment and its risks, is advocated.     

Towards the construction of a resistance risk evaluation scheme

Pesticide resistance management needs an indication of the risk of resistance developing in pests against pesticide applications. This paper describes an evaluation system for the ranking of these risks. The term pests includes all organisms which are causing economic damage in agriculture, including weeds and plant pathogens. The system distinguishes six broad risk categories. It is based on expert judgement of answers to a maximum of ten questions on crop husbandry, pest biology and pest control. The system has been developed for registration purposes in The Netherlands, and is currently being discussed within the European and Mediterranean Plant Protection Organisation (EPPO). ©1997 SCI     

Supervised control of the carrot fly in the Netherlands; insights from a dirty dataset1

‘De Groene Vlieg’ (the Green Fly) is an independent company offering various services concerning integrated pest management in open field agriculture in the Netherlands. One such service is the supervised control of carrot fly (Psila rosae). From 1993 to 2008 the company has monitored the carrot fly population on a total of nearly 65 000 hectares resulting in an extensive dataset. The data was not collected according to proper experimental methods, hence it is titled dirty, but the size of the dataset does allow valuable insights to be gained. De Groene Vlieg monitors the carrot fly population on each field separately. Sticky traps are collected weekly and flies are counted in the laboratory. Growers receive messages containing advice regarding when to apply insecticides and they are responsible for doing so. Overall, over a third of the fields require no spraying at all and for 75% of the fields growers are advised to spray three times or less. One insight from the dataset is that small carrots require more insecticide treatments than large carrots (‘winter carrots’). Reasons for this difference are probably both regional and cultural. Additionally, focusing on a small and uniform area reveals that carrot fly population dynamics are highly variable between years. Another insight is that sowing date has a large effect on the number of insecticide sprayings that are required. Fields sown before the first flight have more flies in the second flight than fields sown later. Comparison of biological and conventional carrot fields shows that carrot fly populations are similar for the first flight. However, during the second flight the number of flies is much higher on organic fields than on conventional ones. Finally, insecticide seed treatment with chlorfenvinphos to control the reproduction of the first flight appears slightly more efficient than supervised control. However, supervised control seems as efficient as seed treatment with clothianidin and beta cyfluthrin.     

Loss of Avirulence and Reduced Pathogenicity of a Gamma-Irradiated Mutant of Fusarium oxysporum f. sp. lycopersici

ABSTRACT The tomato Fusarium resistance gene I-2 confers resistance to F. oxy-sporum f. sp. lycopersici race 2, which expresses the corresponding aviru-lence gene avrI-2. To elucidate the molecular basis of this gene-for-gene interaction, we initiated a search for the avrI-2 gene. Gamma irradiation mutagenesis, using (137)Cs, was performed to generate an avrI-2 mutant of F. oxysporum f. sp. lycopersici. To this end, a race 2 isolate was first transformed with a phleomycine resistance gene and a GUS marker gene in order to distinguish mutants from contaminating isolates. A total of 21,712 mutagenized colonies was tested for loss of avirulence on I-2-containing tomato seedlings. One mutant was selected that showed the expected loss of avirulence but, surprisingly, also showed reduced pathogenicity toward susceptible tomato plants. DNA analysis was subsequently used to visualize genomic changes in the mutant. Southern analysis on contour-clamped homogeneous electrophoretic field blots demonstrated a translocation of a 3.75-Mb chromosome in the mutant. Random amplified polymorphic DNA and amplified fragment length polymorphism analysis identified at least nine polymorphisms between the wild-type and mutant isolates. Most of these polymorphisms appeared as extra fragments in the mutant and contained repetitive DNA sequences.     

鸡马立克氏病Ⅰ型弱毒株的选育

本研究利用从国外引进的鸡马立克氏病Ⅰ型弱毒疫苗株,采取回归无特定病原（ＳＰＦ）雏鸡和在细胞培养物上交替接种的方法,连续传代５次后,进行免疫效力试验的结果表明,本研究选育的ＭＤＶ－Ｇ１毒株含１０,０００和２,０００独斑形成单位（ＰＦＵ）／只鸡剂量试验组对ＭＤ的保护率均达１００％;未经选育的进口ＭＤＩ型弱毒商品疫苗２,０００ＰＦＵ／只鸡剂量试验组,对ＭＤ的保护率为８６．７％;而常规使用的ＨＶＴ商品疫苗     

Myths of slash and burn on physical degradation of savannah soils: impacts on Vertisols in north Cameroon

Abstract. A study was conducted to assess the impact of agricultural land use history on macro-aggregate (4.0–4.8 mm) stability in the 0–5 cm layers of Chromic and 'Hydromorphic' Vertisols in north Cameroon. Macroaggregate stability to water drop impact was determined and the ASI₅₀ index calculated. Macroaggregates from fallow and zero-tilled cropped soils disaggregated in a stepwise manner. Macroaggregates from ploughed cropped soils collapsed in one step into semi-liquefied microaggregates and primary particles. On both soil types, the ASI₅₀ index of samples from ploughed land was 10.0 mJ, compared to 16.4– 21.9 mJ from zero-tilled slash and burn land use. The stepwise disintegration of macroaggregates indicated the existence of a hierarchy of aggregation within the size range 2–5 mm. Slash and burn land use on zero-tilled Vertisols significantly increased sand-sized organic carbon content and the stability of macroaggregates to water impact.     

Analysis of trypsin inhibitors and lectins in white kidney beans (Phaseolus vulgaris, var. Processor) in a combined method.

Buffered saline extraction, affinity chromatography, and Folin-BSA protein assay were used consecutively to provide a combined method for analysis of trypsin inhibitors and lectins in white kidney beans (Phaseolus vulgaris, var. Processor). The method was tested by following the decrease of both antinutritional factors by germination of the beans for 7 days at 20 degrees C. Repeatability coefficients of variation were 2-7.4% for the trypsin inhibitors and 2.2-10% for the lectins. After 7 days of germination, trypsin inhibitors and lectins were reduced by 72 and 92%, respectively.     

Observations of soil water and salt movement under drip and flood irrigation in an apple orchard

Part of a mature apple orchard, previously flood irrigated, was converted to drip irrigation in August 1971 and maintained until May 1975. The remainder of the orchard was irrigated as before.Annual estimates were made of moisture, total soluble salts and chloride content of soil samples and of growth, leaf chloride and yield of trees from the two irrigation treatments. Soil moisture was also monitored with a neutron moisture meter.Very high salt concentrations were observed in the various soil layers in the drip irrigation treatment. The flood irrigation treatment had lower and less variable concentrations of salts.Yields and chloride content of leaves sampled each January did not differ significantly between treatments. Tree girths were smaller under drip irrigation.The drip system, using a low pressure head, filtered water and outlets wrapped in fibreglass, operated satisfactorily for the period of the trial.