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71.
Srinivasan Samineni Pooran M. Gaur Timothy D. Colmer L. Krishnamurthy Vincent Vadez Kadambot H. M. Siddique 《Euphytica》2011,182(1):73-86
Chickpea (Cicer arietinum L.) is known to be salt-sensitive and in many regions of the world its yields are restricted by salinity. Recent identification
of large variation in chickpea yield under salinity, if genetically controlled, offers an opportunity to develop cultivars
with improved salt tolerance. Two chickpea land races, ICC 6263 (salt sensitive) and ICC 1431 (salt tolerant), were inter-crossed
to study gene action involved in different agronomic traits under saline and control conditions. The generation mean analysis
in six populations, viz. P1, P2, F1, F2, BC1P1 and BC1P2, revealed significant gene interactions for days to flowering, days to maturity, and stem Na and K concentrations in control
and saline treatments, as well as for 100-seed weight under salinity. Seed yield, pods per plant, seeds per plant, and stem
Cl concentration were controlled by additive effects under saline conditions. Broad-sense heritability values (>0.5) for most
traits were generally higher in saline than in control conditions, whereas the narrow-sense heritability values for yield
traits, and stem Na and K concentrations, were lower in saline than control conditions. The influence of the sensitive parent
was higher on the expression of different traits; the additive and dominant genes acted in opposite directions which led to
lower heritability estimates in early generations. These results indicate that selection for yield under salinity would be
more effective in later filial generations after gene fixation. 相似文献
72.
Mitton-Fry RM Anderson EM Hughes TR Lundblad V Wuttke DS 《Science (New York, N.Y.)》2002,296(5565):145-147
The essential Cdc13 protein in the yeast Saccharomyces cerevisiae is a single-stranded telomeric DNA binding protein required for chromosome end protection and telomere replication. Here we report the solution structure of the Cdc13 DNA binding domain in complex with telomeric DNA. The structure reveals the use of a single OB (oligonucleotide/oligosaccharide binding) fold augmented by an unusually large loop for DNA recognition. This OB fold is structurally similar to OB folds found in the ciliated protozoan telomere end-binding protein, although no sequence similarity is apparent between them. The common usage of an OB fold for telomeric DNA interaction demonstrates conservation of end-protection mechanisms among eukaryotes. 相似文献
73.
Multimillion-atom molecular-dynamics simulations are used to investigate the shock-induced phase transformation of solid iron. Above a critical shock strength, many small close-packed grains nucleate in the shock-compressed body-centered cubic crystal growing on a picosecond time scale to form larger, energetically favored grains. A split two-wave shock structure is observed immediately above this threshold, with an elastic precursor ahead of the lagging transformation wave. For even higher shock strengths, a single, overdriven wave is obtained. The dynamics and orientation of the developing close-packed grains depend on the shock strength and especially on the crystallographic shock direction. Orientational relations between the unshocked and shocked regions are similar to those found for the temperature-driven martensitic transformation in iron and its alloys. 相似文献
74.
Although critical for development, immunity, wound healing, and metastasis, integrins represent one of the few classes of plasma membrane receptors for which the basic signaling mechanism remains a mystery. We investigated cytoplasmic conformational changes in the integrin LFA-1 (alphaLbeta2) in living cells by measuring fluorescence resonance energy transfer between cyan fluorescent protein-fused and yellow fluorescent protein-fused alphaL and beta2 cytoplasmic domains. In the resting state these domains were close to each other, but underwent significant spatial separation upon either intracellular activation of integrin adhesiveness (inside-out signaling) or ligand binding (outside-in signaling). Thus, bidirectional integrin signaling is accomplished by coupling extracellular conformational changes to an unclasping and separation of the alpha and beta cytoplasmic domains, a distinctive mechanism for transmitting information across the plasma membrane. 相似文献
75.
Maintenance of genomic methylation patterns is mediated primarily by DNA methyltransferase-1 (DNMT1). We have solved structures of mouse and human DNMT1 composed of CXXC, tandem bromo-adjacent homology (BAH1/2), and methyltransferase domains bound to DNA-containing unmethylated CpG sites. The CXXC specifically binds to unmethylated CpG dinucleotide and positions the CXXC-BAH1 linker between the DNA and the active site of DNMT1, preventing de novo methylation. In addition, a loop projecting from BAH2 interacts with the target recognition domain (TRD) of the methyltransferase, stabilizing the TRD in a retracted position and preventing it from inserting into the DNA major groove. Our studies identify an autoinhibitory mechanism, in which unmethylated CpG dinucleotides are occluded from the active site to ensure that only hemimethylated CpG dinucleotides undergo methylation. 相似文献
76.
Fairman ME Maroney PA Wang W Bowers HA Gollnick P Nilsen TW Jankowsky E 《Science (New York, N.Y.)》2004,304(5671):730-734
Members of the DExH/D superfamily of nucleic acid-activated nucleotide triphosphatases are essential for virtually all aspects of RNA metabolism, including pre-messenger RNA splicing, RNA interference, translation, and nucleocytoplasmic trafficking. Physiological substrates for these enzymes are thought to be regions of double-stranded RNA, because several DExH/D proteins catalyze strand separation in vitro. These "RNA helicases" can also disrupt RNA-protein interactions, but it is unclear whether this activity is coupled to duplex unwinding. Here we demonstrate that two unrelated DExH/D proteins catalyze protein displacement independently of duplex unwinding. Therefore, the essential functions of DExH/D proteins are not confined to RNA duplexes but can be exerted on a wide range of ribonucleoprotein substrates. 相似文献
77.
78.
In a suspension of reverse micelles, in which the surfactant sodium dioctyl sulfosuccinate (AOT) separates a water nanodroplet from a bulk nonpolar CCl4 phase, ultrafast vibrational spectroscopy was used to study vibrational energy transfer from the nanodroplet through the AOT interfacial monolayer to the surrounding CCl4. Most of the vibrational energy from the nanodroplet was transferred to the polar AOT head group within 1.8 picoseconds and then out to the CCl4 within 10 picoseconds. Vibrational energy pumped directly into the AOT tail resulted in a slower 20- to 40-picosecond transfer of energy to the CCl4. 相似文献
79.
Canary song is hierarchically structured: Short stereotyped syllables are repeated to form phrases, which in turn are arranged to form songs. This structure occurs even in the songs of young isolates, which suggests that innate rules govern canary song development. However, juveniles that had never heard normal song imitated abnormal synthetic songs with great accuracy, even when the tutor songs lacked phrasing. As the birds matured, imitated songs were reprogrammed to form typical canary phrasing. Thus, imitation and innate song constraints are separate processes that can be segregated in time: freedom in youth, rules in adulthood. 相似文献
80.
Recombination of human mitochondrial DNA 总被引:1,自引:0,他引:1
Kraytsberg Y Schwartz M Brown TA Ebralidse K Kunz WS Clayton DA Vissing J Khrapko K 《Science (New York, N.Y.)》2004,304(5673):981