Genotypic and Pathotypic Diversity in Xanthomonas oryzae pv. oryzae in Nepal

ABSTRACT Among the 171 strains of Xanthomonas oryzae pv. oryzae (the bacterial blight pathogen of rice) collected from eight rice-producing zones in Nepal, 31 molecular haplotypes were distinguished using two polymerase chain reaction-based assays. Six common haplotypes represented nearly 63% of the strains, and some haplotypes were geographically dispersed. Multiple correspondence analysis divided the collection into five putative genetic lineages. Lineages 1, 2, and 4 were the most frequently detected and occurred in diverse geographic populations. Twenty-six pathotypes (virulence phenotypes) of X. oryzae pv. oryzae were identified using 11 near-iso-genic rice lines, each containing a single gene for resistance. The 26 pathotypes grouped into five clusters, and cluster 1 contained wide virulence spectrum strains from all geographic populations. Although molecular variation was greatest between strains of different virulence phenotypes, some variation was observed among strains with identical virulence. There was a weak correlation (r = 0.52) between molecular haplotypes and virulence phenotypes. There are two major groups of X. oryzae pv. oryzae in Nepal. One group consists of strains with high molecular polymorphism and many pathotypes that are either virulent to the 11 major resistance genes or avirulent only to Xa21. Strains in the second group have low molecular polymorphism and are avirulent to Xa4, xa5, Xa7, and Xa21.     

From sharecropping to crop-rent: women farmers changing agricultural production relations in rural South Asia

This paper explores changing production relations in agriculture in context of increasingly widespread and longer-duration male outmigration, as against previous, short-duration and seasonal migration. It investigates how de facto women-heads of households (WHHs) are changing a resilient crop-sharing system in absence of adequate access to productive assets, formal training or experience in farming, and while contributing labour to farming and coping with gendered demands on their time. Based on qualitative inquiry in one of the poorest parts of South Asia, the Eastern Gangetic Plains, the paper shows that a section of WHHs are replacing sharecropping arrangements with fixed-value rental arrangements that resemble commercial contracts. The paper ends with a discussion on the implications of this emerging development.     

Regeneration ecology and population status of a critically endangered and endemic tree species （Ilex khasiana Purk.） in north-eastern India

Detailed studies on population structure and regeneration ecology of Ilex khasiana were carried out at five representative natural populations i.e. Mylliem (Population - I), Upper Shillong (Population - II), Shillong Peak (Population - III), Laitkor (Population - IV) and Nongpiyur (Population - V) to probe into the responsible affecting factors for its population structure and regeneration ability in these populations. Experimental observation under three controlled light conditions (full sunlight (100%), intermediate sunlight (50%) and low sunlight (30%)) showed that the growth status and survival rates of seedlings under intermediate and low light were better than those under the high light condition. The density-diameter distribution in population of adult trees (≥5cm dbh) exhibited a typical character of a regenerating population. A higher seedling mortality rate in natural populations of I. khasiana was most probably due to increase in light intensity following vegetation destruction and other anthropogenic disturbances that cause opening of forest canopy. The findings of the present study would be of immense value in formulating appropriate conservation measures for the species.     

Cost-Benefit Analysis of a Community Forest in Nepal

Community forestry is an approach for mitigating deforestation and forest degradation by managing the forest resources for benefitting neighboring communities. Monetary benefits and costs are associated in a community forest during conservation and management of a forest. For sustainable forest management, the benefit should be more than cost which is a contesting issue of research. So, this study was conducted in a community forest of the central part of Nepal with the help of 80 household surveys and a focus group discussion. The firsthand information collected at the site is complimented by forest product harvest and cost-related secondary information. It was observed that the total annual harvest of timber was 60 cubic feet, pole was 8 cubic feet, firewood was 1,110 Bhari,¹ fodder was 4,388 Bhari, and leaf litter was 590 Bhari. To manage a forest, people were involved in fencing, thinning, and meetings. Management cost was six times higher than administration cost. The benefit from firewood and fodder was more due to the dependency of people in a forest for enhancing their livelihood. The higher value of benefit cost ratio indicates that the Community Forest User Group benefited from community forest management.     

The genetic control of mildly restricted branching in narrow-leafed lupin (Lupinus angustifolius L.)

The indeterminate growth habit of most cultivars of narrow-leafed lupin(Lupinus angustifolius) results in undue competition between podgrowth and branch growth, and sometimes poor partitioning of dry massto grain. Selection of restricted-branching genotypes has been proposedas a method to overcome this problem. The restricted branching (RB)trait occurs in two forms, mild and severe. This paper reports the patternof genetic control of mild RB derived from a number of independentsources. The trait, as indicated by the number of leaves on the uppermostbranch, was found to be quantitative (quasi-continuous), but highlyheritable, and largely controlled by additive genetic effects. There was noconsistent correlation of this trait with the number of leaf nodes on themain stem or the time to flowering. It should therefore be possible toobtain different combinations of branching pattern and maturity datethrough plant breeding, and to assess their adaptation to a range ofenvironments.     

Oocyte-specific deletion of Pten causes premature activation of the primordial follicle pool

In the mammalian ovary, progressive activation of primordial follicles from the dormant pool serves as the source of fertilizable ova. Menopause, or the end of female reproductive life, occurs when the primordial follicle pool is exhausted. However, the molecular mechanisms underlying follicle activation are poorly understood. We provide genetic evidence that in mice lacking PTEN (phosphatase and tensin homolog deleted on chromosome 10) in oocytes, a major negative regulator of phosphatidylinositol 3-kinase (PI3K), the entire primordial follicle pool becomes activated. Subsequently, all primordial follicles become depleted in early adulthood, causing premature ovarian failure (POF). Our results show that the mammalian oocyte serves as the headquarters of programming of follicle activation and that the oocyte PTEN-PI3K pathway governs follicle activation through control of initiation of oocyte growth.