Mineral fertilizers were applied to adjacent plantations of 2, 4-year old, hybrid poplars: clone 27 (Northwest, P. deltoides × P. balsamifera) and 794 (Brooks, P. deltoides × P. × petrowskyana), in north eastern Alberta. Fertilization was done in May 2003 to see whether growth rates could be increased and rotations
shortened. Three fertilizers (N, NP and NPKS + Cu + Zn) were applied at each of three rates (supplying N at 0, 100 and 200 kg ha−1) in a factorial randomized block design to the two separate plantations. Fertilization with 200 kg ha−1 N and 100 kg ha−1 P increased volume growth of clone 27 by 1 m3 ha−1 year−1 over 2 years. Clone 794 showed no volume response to fertilization, but produced 8.7 m3 ha−1 more than clone 27. Leaf area, dry mass and nutrient concentrations of both clones increased in the first year after fertilization,
showing that fertilizer uptake occurred. Decrease in leaf size between 2003 and 2004 was affected by fertilizer level in clone
794. There were differences between some nutrient concentrations in the soils occupied by the two clones, and clone 794 had
higher leaf concentrations of N, K, Ca, S, Mn, Zn, B and Mo than clone 27. Fertilization of 4-year old plantations of either
clone was unwarranted, and planting clone 794 would be likely to provide greater yield than planting and fertilizing clone
27. 相似文献
Predicting ecosystem resilience is a challenge, especially as climate change alters disturbance regimes and conditions for recovery. Recent research has highlighted the importance of spatially-explicit disturbance and resilience processes to long-term ecosystem dynamics. “Neoecological” approaches characterize resilience mechanisms at relatively fine spatio-temporal resolutions, but results are difficult to extrapolate across broad temporal scales or climatic ranges. Paleoecological methodologies can consider the effects of climates that differ from today. However, they are often limited to coarse-grained spatio-temporal resolutions.
Methods
In this synthesis, we describe implicit and explicit examples of studies that incorporate both neo- and paleoecological approaches. We propose ways to build on the strengths of both approaches in an explicit and proactive fashion.
Results
Linking the two approaches is a powerful way to surpass their respective limitations. Aligning spatial scales is critical: Paleoecological sampling design should incorporate knowledge of the spatial characteristics of the disturbance process, and neoecological studies benefit from a longer-term context to their conclusions. In some cases, modeling can incorporate non-spatial data from paleoecological records or emerging spatial paleo-data networks with mechanistic disturbance/recovery processes that operate at fine spatiotemporal scales.
Conclusions
Linking these two complementary approaches is a powerful way to build a complete understanding of ecosystem disturbance and resilience.
Lupinus albus seeds contain conglutin gamma (Cγ) protein, which exerts a hypoglycemic effect and positively modifies proteins involved in glucose homeostasis. Cγ could potentially be used to manage patients with impaired glucose metabolism, but there remains a need to evaluate its effects on hepatic glucose production. The present study aimed to analyze G6pc, Fbp1, and Pck1 gene expressions in two experimental animal models of impaired glucose metabolism. We also evaluated hepatic and renal tissue integrity following Cγ treatment. To generate an insulin resistance model, male Wistar rats were provided 30% sucrose solution ad libitum for 20 weeks. To generate a type 2 diabetes model (STZ), five-day-old rats were intraperitoneally injected with streptozotocin (150 mg/kg). Each animal model was randomized into three subgroups that received the following oral treatments daily for one week: 0.9% w/v NaCl (vehicle; IR-Ctrl and STZ-Ctrl); metformin 300 mg/kg (IR-Met and STZ-Met); and Cγ 150 mg/kg (IR-Cγ and STZ-Cγ). Biochemical parameters were assessed pre- and post-treatment using colorimetric or enzymatic methods. We also performed histological analysis of hepatic and renal tissue. G6pc, Fbp1, and Pck1 gene expressions were quantified using real-time PCR. No histological changes were observed in any group. Post-treatment G6pc gene expression was decreased in the IR-Cγ and STZ-Cγ groups. Post-treatment Fbp1 and Pck1 gene expressions were reduced in the IR-Cγ group but increased in STZ-Cγ animals. Overall, these findings suggest that Cγ is involved in reducing hepatic glucose production, mainly through G6pc inhibition in impaired glucose metabolism disorders. 相似文献
Cassava (Manihot esculenta Crantz) native starch was treated with pyroconversion and enzymatic hydrolysis to produce a pyrodextrin and an enzyme-resistant maltodextrin. Some nutritional characteristics were quantified for both compounds. Pyroconversion was done using a 160:1 (p/v) starch:HCl ratio, 90 °C temperature and 3 h reaction time. The resulting pyrodextrin contained 46.21% indigestible starch and 78.86% dietary fiber. Thermostable α-amylase (0.01%) was used to hydrolyze the pyrodextrin at 95 °C for 5 min. The resulting resistant maltodextrin contained 24.45% dextrose equivalents, 56.06% indigestible starch and 86.62% dietary fiber. Compared to the cassava native starch, the pyrodextrin exhibited 56% solubility at room temperature and the resistant maltodextrin 100%. The glycemic index value for the resistant maltodextrin was 59% in healthy persons. Its high indigestible starch and dietary fiber contents, as well as its complete solubility, make the resistant maltodextrin a promising ingredient for raising dietary fiber content in a wide range of foods, especially in drinks, dairy products, creams and soups. 相似文献
Quinoa protein concentrate (QPC) was extracted and digested under in vitro gastrointestinal conditions. The protein content of QPC was in the range between 52.40 and 65.01% depending on the assay used. Quinoa proteins were almost completely hydrolyzed by pepsin at pH of 1.2, 2.0, and 3.2. At high pH, only partial hydrolysis was observed. During the duodenal phase, no intact proteins were visible, indicating their susceptibility to the in vitro simulated digestive conditions. Zebrafish larvae model was used to evaluate the in vivo ability of gastrointestinal digests to inhibit lipid peroxidation. Gastric digestion at pH 1.2 showed the highest lipid peroxidation inhibition percentage (75.15%). The lipid peroxidation activity increased after the duodenal phase. The digest obtained at the end of the digestive process showed an inhibition percentage of 82.10%, comparable to that showed when using BHT as positive control (87.13%). 相似文献
This work examined the processing yields, physico-chemical characteristics, and bioactive properties of Canadian organic cranberry and blueberry pomace and phenolic-enriched extractives. The ethanol extracts from each berry contained three to four times the soluble solids and sugar contents compared to the pomace. The lipid content of organic cranberry pomace (4.44%), cranberry ethanol extractives (3.86%), and blueberry ethanol extractives (4.44%) were significantly lower than the lipid content of the organic blueberry pomace (5.42%). The organic blueberry pomace showed higher protein content (8.41%) than organic cranberry pomace (5.76%); however, both organic cranberry and blueberry ethanol extracts showed similarly low protein contents. Significant differences were observed in the mineral and elemental contents of both pomaces and their extractives. Total phenolics, tartaric esters, flavonols, anthocyanins, and antioxidant activities were increased by two to three times in both organic cranberry and blueberry ethanol extracts compared to their respective pomaces. The major anthocyanins detected in organic blueberry pomace and ethanol extracts were peonidin 3-glucoside, malvidin 3-galactoside, malvidin 3-glucoside, and cyanidin 3-arabinoside. Peonidin 3-galactoside, cyanidin 3-galactoside, cyanidin 3-arabinoside, and peonidin 3-arabinside were the major anthocyanins in the organic cranberry and ethanol extacts. Results indicated that cranberry and blueberry pomace could be used to develop bioactives with antioxidant activity for potential health benefit and/or bioenergy production. 相似文献