<Emphasis Type="Italic">Cucurbit aphid-borne yellows virus</Emphasis> in Egypt

During 2010, yellowing symptoms were frequently observed in cultivated squash fields in Egypt. A total of 717 symptomatic squash leaf samples were collected from four regions where squash cultivation is of economic importance for the country: Kafrelsheikh, El-Behira, El-Sharkia and El-Ismailia. Serological analysis showed that 95.6% of the symptomatic squash samples were infected by Cucurbit aphid-borne yellows virus (CABYV), and visual estimation of the incidence of yellowing symptoms suggested a very high incidence of CABYV in the fields. Twelve CABYV isolates were characterized by sequencing two regions of the viral genome, open reading frame (ORF) 3 and ORFs 4/5. Overall, Egyptian isolates were very similar among them, and had higher similarity values with a French than with a Chinese isolate. The average nucleotide diversity for ORF 3 was significantly higher than for the other two regions, indicating that variability is not evenly distributed along the viral genome. The ratios between nucleotide diversity values in non-synonymous (d _N ) and synonymous (d _S) positions (d _N /d _S) for each ORF showed that the three ORFs are evolving under different pressures, although predominantly under purifying selection. Phylogenetic analyses revealed that these Egyptian isolates, with only one exception, shared the same clade with a French isolate. Moreover, these analyses suggested that Egyptian isolates belong to the Mediterranean group described previously.     

Identification of <Emphasis Type="Italic">Pythium tracheiphilum</Emphasis> as the causal agent of vascular necrosis of endive (<Emphasis Type="Italic">Cichorium endivia</Emphasis>) in Spain

Pythium isolates were recovered from endive plants (Cichorium endivia) showing vascular necrosis collected from commercial fields located in Castellón province (eastern Spain). They were identified as Pythium tracheiphilum on the basis of their phenotypical and molecular profile. Pathogenicity tests conducted with two P. tracheiphilum isolates, obtained from endive and lettuce (Lactuca sativa), respectively, in this region, confirmed that both isolates were pathogenic to endive, with no significant differences in virulence between them. This is the first report of vascular necrosis caused by P. tracheiphilum on endive in Spain.     

Green-odour compounds have antifungal activity against the rice blast fungus <Emphasis Type="Italic">Magnaporthe oryzae</Emphasis>

Four green-odour compounds—trans-2-hexenal, cis-3-hexenol, n-hexanal, and cis-3-hexenal—were applied (0.85 μg ml⁻¹ as vapour) to rice plants in laboratory conditions to observe their biological activity against the phytopathogenic fungus Maganporthe oryzae, which causes rice blast disease worldwide. Two compounds, trans-2-hexenal and cis-3-hexenal, showed remarkable disease suppression efficacy (99.7% and 100% suppression, respectively), while n-hexanal had moderate (86.5%) and cis-3-hexenol had weak (20.8%) disease-suppressing effects. Pre-application and post-application of trans-2-hexenal or cis-3-hexenal had slight effects on blast incidence, suggesting that these compounds had direct effects to suppress M. oryzae infection. In fact, trans-2-hexenal and cis-3-hexenal exhibited a growth suppression effect on M. oryzae. Interestingly, these two compounds inhibited appressorium formation at lower concentrations than the growth suppression. Studies on the hypersensitive response (HR)-like reaction and plant β-1,3-glucanase activity in rice plant confirmed that induced resistance was not the major factor involved in the disease suppression mechanism. Results of this study conclusively showed that trans-2-hexenal and cis-3-hexenal possess potent inhibitory activities against the growth and the appressorium formation of M. oryzae and could be used as antifungal agents to significantly reduce M. oryzae infections in rice.     

Airborne fungal succession in a rice field of Cuba

This study characterised the airborne mycobiota of a rice plantation in Bauta (Havana, Cuba), with a view to improving crop quality. Culturable airborne fungi were sampled between March 2007 and February 2008 using a volumetric method (SAS Super 100) with potato dextrose agar as culture medium. The total fungal count was 51,425 cfu/m³. The 39 filamentous genera identified included several major rice pathogens (Bipolaris, Curvularia, Alternaria, Pyricularia, Cercospora and Fusarium) as well as potentially mycotoxigenic species of Fusarium, Penicillium and Aspergillus. Analysis of the influence of weather-related factors on spore counts revealed that total counts and Penicillium counts correlated positively with daily relative humidity, mean temperature and dew point.     

Frequency of mutations associated with fungicide resistance and population structure of <Emphasis Type="Italic">Mycosphaerella graminicola</Emphasis> in Tunisia

The occurrence of fungicide resistance in Mycosphaerella graminicola populations from Tunisia was investigated by examining mutations known to be associated with strobilurin and azole resistance. Few mutations associated with fungicide resistance were detected. No evidence for strobilurin resistance was found among 357 Tunisian isolates and only two among 80 sequenced isolates carried mutations associated with azole resistance. A network analysis suggested that these mutations emerged independently from mutations found in previously described European populations. The population genetic structure of M. graminicola in Tunisia was analyzed using variation at 11 microsatellite loci. Populations in Tunisia were characterized by high gene and genotype diversity. All populations were in gametic equilibrium and mating type proportions did not deviate from the 1:1 ratio expected under random mating, consistent with regular cycles of sexual reproduction. In combination with a high degree of gene flow among sampling sites, M. graminicola must be considered a pathogens with high evolutionary potential. Thus, control strategies against Septoria blotch in Tunisia should be optimized to reduce the emergence and spread of resistant isolates.     

Estimating genetic effects in maternal and paternal half-sibs from tetraploid-diploid crosses in <Emphasis Type="Italic">Musa</Emphasis> spp.

Ploidy manipulations and interspecific crosses have allowed considerable genetic progress in Musa breeding, but estimation of genetic parameters for parental selection remains a major challenge. This study aimed to determine the components of genetic variance and the relative contribution of genetic effects to phenotypic variation of yield and phenological traits in secondary triploid hybrids from tetraploid–diploid crosses. The hybrids were evaluated in two consecutive growth cycles on a tropical forest site. Non-genetic effects accounted for a large fraction of the variation observed for most traits, except bunch weight. Partitioning of genetic variance into additive and dominance components confirmed the predominant role of additive genetic effects on the expression of bunch weight, fruit filling time, fruit length, plant height, and number of leaves while primarily non-additive effects accounted for suckering behavior and fruit circumference. Maternal general combining ability (GCA) effects accounted for the additive genetic variation in plant height and number of leaves, suggesting that selection for these traits should be carried out in tetraploid clones. Conversely, paternal GCA effects were the primary causes of genetic variation for fruit filling time, bunch weight, and fruit length, suggesting that these characters should be selected for in diploid clones. Specific combining ability (SCA) effects were observed for all traits, except fruit filling time, suggesting that additional genetic gain could be achieved through recombinative heterosis for these traits.     

Inheritance of anthocyanin content in the ripe berries of a tetraploid × diploid grape cross population

Variation patterns and inheritance of anthocyanin content in the ripe berries of a tetraploid × diploid table grape cross population were investigated in two successive years. The population segregated for three different ploid levels: dipolids, triploids, and tetraploids. A total of 28 different anthocyanins were detected and quantified in the progeny population. Transgressive segregation for the total anthocyanin content was observed in all the three ploid progeny populations. The total anthocyanin content increased as the ploid level increased. The broad sense heritabilities (H²) of the total anthocyanin content were all relatively high, ranging from 0.53 to 0.98, 0.57 to 0.97 and 0.43 to 0.94 in the diploid, triploid and tetraploid population, respectively. Our results suggested that the total anthocyanin content followed an additive inheritance model in this polyploid segregation population. We also observed that the relative contribution of individual anthocyanins to the total anthocyanin content varied significantly among different ploid populations, suggesting that genetic background has important impact on the accumulation of the individual anthocyanin compounds. These results will help develop better breeding strategies in a polyploid table grape breeding program for improving the content of anthocyanins, an important class of polyphenolics possessing antioxidant activities and many other health-related benefits.     

Mapping quantitative trait loci for cottonseed oil,protein and gossypol content in a <Emphasis Type="Italic">Gossypium hirsutum</Emphasis> × <Emphasis Type="Italic">Gossypium barbadense</Emphasis> backcross inbred line population

Cotton is one of the most important oil-producing crops and the cottonseed meal provides important protein nutrients as animal feed. However, information on the genetic basis of cottonseed oil and protein contents is lacking. A backcross inbred line (BIL) population from a cross between Gossypium hirsutum as the recurrent parent and G. barbadense was used to identify quantitative trait loci (QTLs) for cottonseed oil, protein, and gossypol contents. The BIL population of 146 lines together with the two parental lines was tested in the same location for three years in China. Based on a genetic map of 392 SSR markers and a total genetic distance of 2,895.2 cM, 17 QTLs on 12 chromosomes for oil content, 22 QTLs on 12 chromosomes for protein content and three QTLs on two chromosomes for gossypol content were detected. Seed oil content was significantly and negatively correlated with seed protein content, which can be explained by eight QTLs for both oil and protein contents co-localized in the same regions but with opposite additive effects. This research represents the first report using a permanent advanced backcross inbred population of an interspecific hybrid population to identify QTLs for seed quality traits in cotton in three environments.     

Combining ability for grain chemistry quality traits in a white oat diallelic cross

There has been a strong demand for oat genotypes that contain caryopsis with high chemical quality which can suit the different market niches. Therefore, the objectives of this study were to assess the general (GCA) and specific combining ability (SCA) of white oat cultivars through diallelic crosses providing information about the genetic effects on expression of grain chemical quality components. Also, it was aimed to estimate the heterosis on F₁ and F₂ generations and the vigor loss due to inbreeding. During 2008, 21 hybrid populations F₁ and F₂ were obtained from artificial crossing among seven Brazilian white oat cultivars, following the complete diallel design, without considering the reciprocals. These populations and their parents were evaluated in the 2009 season in the experimental field in Capão do Leão, RS, Brazil. The higher values of mean squares associated to GCA indicates a strong contribution of additive genetic effects to the expression of grain chemical components. The parents tested showed a tendency to develop progeny with negative heterosis regarding protein, lipid, β-glucan and soluble dietary fiber in the grain, and positive for the content of nitrogen-free extract, total and insoluble dietary fiber. IAC 7 features a potential parent for obtaining grains with high protein and dietary fiber content, and low caloric content, fit to human diet. Meanwhile, UPF 15 and FAPA Louise can represent donors of alleles to increase lipid contents, while FAPA Louise and URS Guapa can be used to raise the grain nitrogen-free extract contents of lines intended for animal feeding.