Infection frequency of mature apple fruit with Erwinia amylovora deposited on pedicels and its survival in the fruit stored at low temperature

The infection frequency of mature apple fruit by Erwinia amylovora and the survival of E. amylovora in the fruit stored at low temperature were investigated. The fruit stems (pedicels) of 460 mature apple fruit were inoculated with 10⁵ or 10⁴ cfu of bioluminescent E. amylovora, tagged with lux genes. Nine days after inoculation, 43% and 27% of the fruit inoculated with 10⁵ and 10⁴ cfu, respectively, were infected. All infected fruit looked healthy. After 6 months of storage at 5°C, almost all of the 142 infected fruit had viable E. amylovora. Of the fruit containing E. amylovora internally, 19.5% had latent infections and the rest had blight symptoms. E. amylovora was not uniformly distributed in the fruit flesh, and internal brown lesions were observed where E. amylovora was densely distributed. These findings showed that mature apple fruit may be infected with E. amylovora, especially as latent infections, and act as a source for long-range dissemination.     

Analysis of water management structures of an Integrated Water User Group in the Chao Phraya Delta, Thailand

An Integrated Water User Group (IWUG) plays an important role assisting an irrigation project to control water distribution, expand the cropping area, operate and maintain irrigation facilities, and disseminate crucial information about the water situation to all farmers based on water supply from an irrigation agency. In this article, we present our analysis of the management of a newly established IWUG, based on a field survey in Thailand. We attempt to clarify the characteristics of irrigation management in the IWUG, while focusing on four functional processes: decision, operation, monitoring, and feedback. Thus, we analyze the water management structure and provide suggestions for better management of the IWUG. The main results of our analysis are as follows: (1) the current state of the IWUG 18R canal is not fully successful. There are second generation problems that need to be solved; (2) The upstream farmers dominate the use of the IWUG 18R canal because the establishment process of downstream WUGs was loosely performed because of a limited budget for on-farm irrigation development; (3) Water distribution structure and membership charging are not simple and uniform along the lateral irrigation canal because of the water availability of return flow from the downstream area. Such structure should be recognized and discussed to improve the future water distribution in relation to membership fees.     

Erwinia amylovora can pass through the abscission layer of fruit-bearing twigs and invade apple fruit during fruit maturation

Invasion of apple fruit by Erwinia amylovora from fruit-bearing twigs through the abscission layer at fruit maturation was examined. Erwinia amylovora (ca. 10⁵ cfu) tagged with bioluminescence genes from Vibrio fischeri was deposited in artificial wounds on fruit-bearing twigs of apple trees grown in a containment greenhouse on September 22, 27, or October 5, 2004. On October 22, 176 apples were harvested and cut horizontally in half. The upper halves were stamped on plates of selective medium, and the lower halves were flooded with iodine solution to assess maturity. All fruit were symptomless and fully mature. The pathogen was recovered from 19 (10.8%) apples. The result showed that if at least ca. 10⁵ cfu of E. amylovora are present in fruit-bearing twigs at the time of fruit maturation, the bacteria can pass through the abscission layer into the fruit, even though the mature fruit lack symptoms.     

A new direction in international cooperation, utilizing Japan’s experience and expertise in the field of agricultural and rural development

There is an urgent need for the international community to address not only the issues of preserving the global environment and reducing poverty in developing countries, but to also face up to new challenges, such as providing assistance to the reconstruction in Afghanistan. Furthermore, it is necessary to address these issues and challenges by means of mutual collaboration. This latter point has been the subject of lively discussion.In this paper, we describe a new direction in international cooperation taken by the Rural Development Bureau of the Ministry of Agriculture, Forestry and Fisheries, Japan in response to the trend of international discussions. This shoulb be achieved by utilizing Japans experience and expertise gained in the development of its own paddy rice agriculture.Specifically, based on the philosophy that sustainable agricultural and rural development can conserve a regional environment, the Bureau intends to undertake: (1) extension of the results from a cooperation project to other regions, by modifying it and converting it to multi-purpose use; (2) collaboration with aid-related entities in Japan and abroad, and dissemination of related information to them; and (3) promotion of cooperation focused on a specific topic.Furthermore, the Bureau will promote the following matters as the basis for international cooperation in each major task. (1) Contribution towards sustainable water use. (2) Extension of the participatory integrated rural development, and its modification for reconstruction assistance. (3) Extension of desertification control, and its modification and conversion for multi-purpose use. (4) Conversion of the technology for soil erosion control into multi-purpose use.