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31.
32.
Christiane von der Ohe Victoria Gauthier Lily Tamburic-Ilincic Anita Brule-Babel W. G. Dilantha Fernando Randy Clear Todd J. Ward Thomas Miedaner 《European journal of plant pathology / European Foundation for Plant Pathology》2010,127(3):407-417
Twenty four isolates of Fusarium graminearum, half of which were 3-acetyldeoxynivalenol (3-ADON) and half 15-acetyldeoxynivalenol (15-ADON) chemotypes, were tested for
their ability to produce deoxynivalenol and to cause Fusarium head blight (FHB) in spring wheat cultivars. The objectives
of this study were to determine (1) whether 3-ADON isolates differ in aggressiveness, as measured by the FHB index, and DON
production from 15-ADON isolates under field conditions, and (2) whether the performance of resistant host cultivars was stable
across isolates. Field tests of all isolates were conducted with three replicates at each of two locations in Canada and Germany
in 2008 with three host genotypes differing in FHB resistance level. The resistant host genotype showed resistance regardless
of the chemotype or location. The differences between mean FHB indices of 3-ADON and 15-ADON isolates were not significant
for any wheat genotype. In contrast, average DON production by the 3-ADON isolates (10.44 mg kg−1) was significantly (P < 0.05) higher than for the 15-ADON isolates (6.95 mg kg−1) at three of the four locations where moderately resistant lines were tested, and at both locations where susceptible lines
were evaluated. These results indicate that 3-ADON isolates could pose a greater risk to food safety. However, as the mean
aggressiveness and DON production of 3-ADON and 15-ADON chemotypes was similar on highly resistant lines, breeding and use
of highly resistant lines is still the most effective measure of reducing the risks associated with DON in wheat. 相似文献
33.
Cristina E. Almeida-Naranjo Patricio J. Espinoza-Montero Marcelo I. Muñoz-Rodríguez Cristina A. Villamar-Ayala 《Water, air, and soil pollution》2017,228(7):253
Hydraulic retention time (HRT) influence improving sludge flocculation with adding the polyelectrolytes (non-ionic, anionic, and cationic) was studied on an activated sludge (AS) system fed with synthetic domestic wastewater (SDW), dairy industry wastewater (DIW), and caramel industry wastewater (CIW). The sludge volumetric index, food/microorganism ratio (F/M), and mixed liquor volatile suspended solids at different HRTs (6, 8 and 10 h) were monitored on an experimental model. Results showed that both SDW and IW had the best sludge flocculation conditions at 8 h and 100 mL of non-ionic polyelectrolyte (0.2 mg L?1). In addition, this phenomenon reached the organic matter removal efficiencies of 95.9, 95.7, and 94.2% for SDW, DIW, and CIW, respectively. Therefore, optimum HRT increased the organic matter removal efficiencies by 10%, sludge concentration by 37% (22–55%), and F/M ratio by 70%. Moreover, the polyelectrolytes used in AS improved the sludge flocculation by 2.9 times. 相似文献
34.
Sakudo A Tsenkova R Tei K Morita H Ikuta K Onodera T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(12):1375-1378
The risk of infections from zoonotic pathogens of tissues and/or tissue-derived products has been increasing. One preventive approach in reducing infection risk is tissue decontamination, where selection and screening of highly infectious tissues are strictly followed. Therefore, the development of reliable analytical methods for rapid tissue discrimination is essentially important. In the present study, a procedure has been developed for intact tissue discrimination on the basis of multivariate analysis of visible and near-infrared (Vis-NIR) spectra of certain tissues such as brain, liver, kidney and testis of mice without any pretreatment. Transmittance spectra in the 600- to 1000-nm regions were subjected to a principal component analysis (PCA), and leave-out cross-validation was employed to develop multivariate models for tissue discrimination. The plot of PCA scores against Vis-NIR spectra of brains, kidneys, livers and testes from 11 mice portrayed reliable tissue discrimination. This result suggests that Vis-NIR spectroscopy combined with chemometrics analysis may provide a potentially useful approach for rapid non-destructive discrimination of tissues. 相似文献
35.
Neutral models for testing landscape hypotheses 总被引:5,自引:0,他引:5
Neutral landscape models were originally developed to test the hypothesis that human-induced fragmentation produces patterns
distinctly different from those associated with random processes. Other uses for neutral models have become apparent, including
the development and testing of landscape metrics to characterize landscape pattern. Although metric development proved to
be significant, the focus on metrics obscured the need for iterative hypothesis testing fundamental to the advancement of
the discipline. We present here an example of an alternative neutral model and hypothesis designed to relate the process of
landscape change to observed landscape patterns. The methods and program, QRULE, are described and options for statistical
testing outlined. The results show that human fragmentation of landscapes results in a non-random association of land-cover
types that can be describe by simple statistical methods. Options for additional landscape studies are discussed and access
to QRULE described in the hope that these methods will be employed to advance our understanding of the processes that affect
the structure and function in human dominated landscapes. 相似文献
36.
Yuichi Kinoshita Katsuhiko Yoshizawa Yuko Emoto Michiko Yuki Takashi Yuri Nobuaki Shikata Susan A. Elmore Airo Tsubura 《Journal of toxicologic pathology》2016,29(1):53-59
Primary ovarian tumors are generally uncommon in rats used in toxicologic studies. A malignant Sertoli cell tumor was present in the ovary of a 19-week-old female Sprague Dawley rat. Macroscopically, the mass was white and firm, 10 × 13 × 17 mm in size, and located in the right ovary. Histopathologically, the mass was composed of nests of pleomorphic cells, which formed seminiferous-like tubules separated by a thin fibrovascular stroma. The tubules were lined by tumor cells, which had basally located nuclei and abundant eosinophilic and vacuolated cytoplasm. In some areas, the tumor cells were arranged in a retiform growth pattern, mimicking a rete testis/ovarii. Disseminated metastases to the surfaces of the mesentery, spleen and liver were also present. Immunohistochemically, many tumor cells were strongly positive for vimentin, estrogen receptor α and Ki 67. Some tumor cells were positive for pancytokeratin and inhibin α. These findings closely resemble those of an ovarian-derived human malignant Sertoli cell tumor. From our review of the literature, we believe this is the first report of a spontaneous malignant Sertoli cell tumor in the ovary of a young laboratory rat. This case might provide useful historical control information for rat toxicity studies. 相似文献
37.
Sawai K Takahashi M Fujii T Moriyasu S Hirayama H Minamihashi A Hashizume T Onoe S 《The Journal of reproduction and development》2011,57(2):236-241
DNA methylation is an important factor for the regulation of gene expression in early embryos. It is well known that the satellite I sequence is more heavily methylated in bovine somatic cell nuclear transfer (NT-SC) embryos than in embryos derived from in vitro fertilization (IVF). However, the methylation status of bovine embryos obtained by other procedures is not well known. To clarify DNA methylation levels of bovine embryos obtained from various procedures, we examined satellite I sequences in bovine blastocyst (BC) embryos derived from NT-SC, NT using embryonic blastomeres (NT-EM), in vivo (Vivo), IVF and parthenogenetic treatment (PA). Furthermore, in order to evaluate the efficacy of DNA demethylation by the NT procedure, we determined the DNA methylation levels in bovine embryos in which NT was recapitulated (Re-NT). Although the DNA methylation levels in the NT-SC embryos were higher than those in the other embryos, the NT-EM embryos exhibited lower DNA methylation levels. The satellite I sequence in the NT-SC embryos was more demethylated than that in the donor cells. Although the DNA methylation level in the individual NT-SC embryos showed variation, the full-term developmental efficacy of these embryos were not different. These findings suggest that the methylation level of the satellite I sequence at the BC stage is not related to the abnormalities of bovine embryos produced by NT-SC. There was no difference in methylation levels between Re-NT and NT-SC embryos. Our results indicated that the DNA methylation status differed among embryos produced by various methods and that at least some of the demethylation of the donor cell genome occurred in the recipient cytoplast after NT-SC, but the demethylation ability of the NT procedure was noted in the first NT but not in the second NT. 相似文献
38.
Seong-Mi Jo Yu Ayukawa Sung-Hwan Yun Ken Komatsu Tsutomu Arie 《Journal of General Plant Pathology》2018,84(6):395-398
RNA silencing pathways in filamentous fungi are composed of multiple component proteins and known to be involved in vegetative growth, virulence or sexual reproduction. We found that the tomato wilt fungus, Fusarium oxysporum f. sp. lycopersici (Fol), carries four homologues genes of Qde-2, an argonaute protein gene and one of the main component protein genes in Neurospora crassa. Gene targeting revealed that FoQde-2, one of the Qde-2 homologues in Fol, is involved in virulence to tomato but not in vegetative growth. 相似文献
39.
40.
Rogério M. Borges Alcester Mendes Selene S. C. Nogueira Jérôme Bindelle Sérgio L. G. Nogueira-Filho 《Tropical animal health and production》2017,49(7):1353-1359
A nitrogen (N) balance digestion trial was conducted to determine the protein requirement of collared peccaries (Pecari tajacu). In a 4 × 4 Latin square design, four captive adult male peccaries were fed four isoenergy diets containing four different levels of N (11.7, 16.3, 22.8, and 26.7 g N/kg of dry matter—DM). After 15 days of adaptation, a total collection of feces and urine was carried out for five consecutive days. Regression analyses between N intake and N in feces and urine allowed to calculate the metabolic fecal nitrogen (MFN = 2.3 g N/kg of dry matter intake—DMI) and daily endogenous urinary N (EUN = 185 mg N/kg0.75). Likewise, by regression analyses between consumption of nitrogen and the nitrogen balance (NB = N ingested ? N excreted, mg N/kg0.75), a daily requirement of 514 mg N/kg0.75 was calculated. Therefore, if food intake is unrestricted, collared peccaries require a minimum in their diet of about 5.4% crude protein on DM basis. These values are almost as low as those found for browsing and frugivorous wild ruminants, which reinforce the proposition that peccaries’ digestive physiology is nearer to that of domestic and wild ruminants than domestic pigs. This relatively low protein requirement of collared peccary and its great ability to digest protein reveal the relevance of the forestomach for the species on nitrogen/protein metabolism and allow the use of diets with lower crude protein levels than the commercial ones used for the domestic pig, which reduces feed costs. 相似文献