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361.
Sato M Hosokawa T Yamaguchi T Nakano T Muramoto K Kahara T Funayama K Kobayashi A Nakano T 《Journal of agricultural and food chemistry》2002,50(21):6245-6252
Seven kinds of angiotensin I-converting enzyme (ACE) inhibitory peptides were isolated from the hydrolysates of wakame (Undaria pinnatifida) by Protease S "Amano" (from Bacillus stearothermophilus) by using three-step high-performance liquid chromatography (HPLC) on a reverse-phase column. These peptides were identified by amino acid composition analysis, sequence analysis, and liquid chromatography-mass spectrometry (LC-MS), as Val-Tyr (IC(50) = 35.2 microM), Ile-Tyr (6.1 microM), Ala-Trp (18.8 microM), Phe-Tyr (42.3 microM), Val-Trp (3.3 microM), Ile-Trp (1.5 microM), and Leu-Trp (23.6 microM). These peptides have resistance against gastrointestinal proteases in vitro. Each peptide was determined to have an antihypertensive effect after a single oral administration in spontaneously hypertensive rats (SHR). Among them, the blood pressure significantly decreased by Val-Tyr, Ile-Tyr, Phe-Tyr, and Ile-Trp in a dose of 1 mg/kg of body weight (BW). The present study showed that antihypertensive effect in the hydrolysates of wakame by Protease S "Amano" was attributed to these peptides. 相似文献
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365.
Masaki MAEZAWA Ai NAKAMICHI Nao AKIYAMA Michihito TAGAWA Ken-ichi WATANABE Yoshiyasu KOBAYASHI Hisashi INOKUMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1643
A 4-year, 7-month-old Holstein cow presented with anorexia. Physical examination revealed masses in the interscapular region and vagina. Blast cells were detected in the masses and peripheral blood by fine needle aspiration cytology and hematological examination. By bone marrow aspiration, blast cells constituted up to 24.2% of all nucleated cells, and 22% and 2% of non-erythroid cells stained positive for myeloperoxidase and alpha-naphthyl acetate esterase (ANAE), respectively. Pathological examination revealed the mass lesions consisted of a proliferation of tumor cells, which were positive for monocytic markers (HLA-DR and Iba-1). The cow was diagnosed with acute myelomonocytic leukemia (AMML). Even when tumor cells are ANAE-negative, AMML cannot be completely ruled out and should be considered when diagnosing cattle with leukemia/lymphoma. 相似文献
366.
Kazufumi OSAKO Akira YAMAGUCHI Takao KUROKAWA Koichi KUWAHARA Hiroaki SAITO Yukinori NOZAKI 《Fisheries Science》2002,68(3):587-594
ABSTRACT: The chemical components and body color of horse mackerel caught between July 1997 and May 2000 were studied. The fish were caught with small- to medium-sized purse seine offshore from Nagasaki and from Tsushima, and with bull trawl seine and medium- to large-sized purse seine in the East China Sea. The crude lipid content of the fish caught offshore from Tsushima was higher than that of the others, and there were no significant differences among the other catches. The extractive nitrogen content of the fish caught with bull trawl seine was somewhat lower than that of the others. The body colors of the fish differed from those suggested by their common names: Kuroaji (black) , Kiaji (yellow), and Shiroaji (white). The crude lipid content and body color indicated that there was no difference in quality among the catches, with the exception of the crude lipid content of the Tsushima catch. 相似文献
367.
Kazuko OGATA Akira IMAI Shinya SATO Kagetomo NISHINO Shinya WATANABE Tamas SOMFAI Eiji KOBAYASHI Kumiko TAKEDA 《The Journal of reproduction and development》2022,68(1):53
During cryopreservation, spermatozoa may suffer cold and cryo-induced injuries ―associated with alterations in cell defense systems― that are detrimental to their function and subsequent fertility. This study aimed to determine the efficacy of supplementing the semen freezing extender with the antioxidant reduced glutathione (GSH) in cattle. Semen was collected from four bulls and diluted in a freezing extender supplemented with or without GSH (0, 1, 5, and 10 mM) before the cooling step of the cryopreservation process. After thawing, the quality of the frozen-thawed semen was investigated for motility, viability, acrosomal and DNA integrity, and subsequent embryo development after in vitro fertilization of bovine oocytes. Additionally, semen from one of the bulls was used to analyze semen antioxidative potential, sperm penetration into oocytes, male pronucleus formation rate, and embryo DNA integrity. The sperm quality varied among bulls after GSH supplementation. One bull had decreased sperm total motility, and two bulls had decreased sperm DNA integrity. GSH supplementation had positive effects on embryo development for three bulls. Two of them showed both improved cleavage and blastocyst formation rates, while the other one only showed an improved cleavage rate. We observed positive effects on early male pronucleus formation and no negative effects on DNA integrity and cell number in blastocyst stage embryos. Although the effect varies depending on individual bulls and GSH concentration, GSH supplementation in semen may improve in vitro embryo production from frozen semen. 相似文献
368.
Yui KOBAYASHI Tatsuro NAKAMURA Tomohiro YONEZAWA Koji KOBAYASHI Takahisa MURATA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(12):1977
Bacterial cystitis is one of the feline lower urinary tract diseases (FLUTDs). Polyunsaturated fatty acids, such as arachidonic acid (ARA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA), are oxidized into various lipid mediators that modulate inflammation. Since the profile of lipid metabolites excreted in urine is useful for assessing inflammatory body conditions, we analyzed 126 types of urinary lipid metabolites in cats with bacterial cystitis. Using LC-MS/MS, we found that the levels of 11 metabolites were higher in the urine of cystitis cats than in the urine of healthy cats. In detail, the urinary levels of ARA, EPA, and DHA and eight of their metabolites were increased in cystitis cats. Focusing on the lipid oxidation pathway, the urinary levels of four cyclooxygenase-, three lipoxygenase-, and one cytochrome P450-dependent oxidated metabolites were increased in bacterial cystitis. These urinary lipid profiles can provide some insight into the pathology and future diagnosis of bacterial cystitis. 相似文献
369.
Takao Katase 《Soil Science and Plant Nutrition》2013,59(4):421-427
Abstract We studied two unknown substances which were produced by incubation of p-coumaric and ferulic acids in a peat soil extract solution. The substances were identified as the cisforms of these acids, which were isomerized from their trans-forms during the incubation by exposure to fluorescent light. Such isomerization did not take place during incubation under dark conditions. After 5 days of incubation by exposure to fluorescent light, the maximal ratio of cis-form to trans-form p-coumaric and ferulic acids was 0.61 and 0.45, respectively. 相似文献
370.
Wataru YAMAMOTO Yasuhiro ITANO Tsunefumi KOBAYASHI Daishiro MIURA Yoshinori KASAHARA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):77-82
Human immunoglobulin G (IgG) concentrates are immune-modulating,
anti-inflammatory plasma-derived products. Clinical studies in recent years have suggested
that IgG attenuates neuropathic pain. In this study, effects of sulphonated IgG on the
development and maintenance of a mechanical allodynia-like response were examined in mice
with neuropathic pain induced by a partial sciatic nerve ligation (PSL). When sulphonated
IgG (400 or 1,000 mg/kg/day, i.p.) was administered for 5 days, from 1 day before surgery
to post-operative day (POD) 3, the development of a mechanical allodynia-like response was
attenuated. On the other hand, sulphonated IgG had little effect on the maintenance of a
mechanical allodynia-like response when administered for 5 days, from POD 11 to POD 15, at
which time a mechanical allodynia-like response had already been developed. To explore the
mechanism of sulphonated IgG, the mRNA expression of inflammatory cytokines was evaluated
in the injured sciatic nerve. Sulphonated IgG (1,000 mg/kg/day, i.p.) that was
administered for 3 days, from 1 day before surgery to POD 1, significantly attenuated the
up-regulation of tumor necrosis factor-α and monocyte chemotactic protein-1 mRNAs on POD
1. These results suggest that prophylactic treatment with sulphonated IgG attenuates the
development of mechanical allodynia-like response by inhibition of inflammatory cytokine
expression in mice with PSL. 相似文献