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51.
Helge Sierotzki Sandro Parisi Ute Steinfeld Isabel Tenzer Sylvie Poirey Ulrich Gisi 《Pest management science》2000,56(10):833-841
Field isolates of Mycosphaerella fijiensis, causing black Sigatoka of banana, were characterised for their sensitivity to different inhibitors of the cytochrome bc1 enzyme complex (Qo respiration inhibitors, strobilurin fungicides), using physiological, biochemical and molecular genetic methods. Strobilurin‐resistant isolates exhibited very high resistance factors both in mycelial growth inhibition and NADH consumption assays. Cross‐resistance was observed among all Qo inhibitors, including trifloxystrobin, azoxystrobin, famoxadone, strobilurin B and myxothiazol. However, the Qi and the cytochrome aa3 inhibitors, antimycin A and potassium cyanide, respectively, were not cross‐resistant to Qo inhibitors. In sensitive but not in resistant isolates, mixtures of Qo inhibitors and SHAM, an inhibitor of the alternative oxidase (AOX), were more active than the components alone, indicating that the alternative pathway is essential in metabolism, but not causal for resistance. In the cell‐free NADH‐consumption assay, the Qo inhibitors affected the sensitive but not the resistant isolates, suggesting that AOX was not active in sub‐mitochondrial particles. In whole cells, however, the AOX has a basic expression level and is probably not inducible by trifloxystrobin. Sequencing of the cytochrome b gene of sensitive and resistant M fijiensis isolates revealed a difference in the nucleotide sequence leading to a single amino acid change from glycine to alanine at position 143 in the resistant isolate. This change is known to occur also in the naturally tolerant basidiomycete Mycena galopoda. It is suggested that the field isolates of M fijiensis can acquire resistance to Qo inhibitors due to a target site alteration with a single base pair change. Resistant isolates do not seem to contain a mixture of mutated and non‐mutated DNA, indicating a complete selection of resistant mitochondria and a maternally donated mode of resistance. © 2000 Society of Chemical Industry 相似文献
52.
Susceptibility in field populations of codling moth,Cydia pomonella (L.) (Lepidoptera: Tortricidae), in Ontario and Quebec apple orchards to a selection of insecticides
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53.
Condensed tannins (CT) can strongly affect litter decomposition, but their fate during the decomposition process, in particular as influenced by detritivore consumption, is not well understood. We tested the hypothesis that litter CT are reduced by the gut passage of two functionally distinct detritivores of Mediterranean forests, the millipede Glomeris marginata, and the land snail Pomatias elegans, as a fixed proportion of initial litter CT, but more so in Pomatias since snails are known to have a more efficient enzymatic capacity. Contrary to our hypothesis, both detritivore species reduced litter CT to near zero in their faecal pellets irrespective of the wide range in initial leaf litter CT concentrations of 9-188 mg g−1 d m among three Mediterranean tree species (Pistacia terebinthus, Quercus ilex, Alnus glutinosa) and different decomposition stages of their litter. The almost complete disappearance of CT even from some litter types highly concentrated in CT, due to either degradation by gut microorganism or complexation of CT into insoluble high molecular weight structures, suggests a high “de-tanning” efficiency across functionally distinct detritivore species. The transformation of CT-rich litter into virtually CT-free faecal pellets by detritivores might be highly relevant for the subsequent decomposition process in ecosystems with a high macrofauna abundance and CT-rich plant species such as Mediterranean forests. 相似文献
54.
Céline Leroy Sylvie Sabatier Novi Sari Wahyuni Jean-François Barczi Jean Dauzat Marilyne Laurans Daniel Auclair 《Agroforestry Systems》2009,77(1):37-47
In agroforestry systems, the distribution of light transmitted under tree canopies can be a limiting factor for the development
of intercrops. The light available for intercrops depends on the quantity of light intercepted by tree canopies and, consequently,
on the architecture of the tree species present. The influence of tree architecture on light transmission was analysed using
dynamic 3D architectural models. The architectural analysis of Acacia mangium and Tectona grandis was performed in Indonesian agroforestry systems with trees aged from 1 to 3 years. 3D virtual trees were then generated
with the AmapSim simulation software and 3D virtual experiments in which tree age, planting density, planting pattern and
pruning intensity varied were reconstructed in order to simulate light available for the crop. Canopy closure of trees was
more rapid in A. mangium than in T. grandis agroforestry systems; after 3 years the quantity of light available for A. mangium intercrops was three times lower than under T. grandis. Simulations with A. mangium showed that practices such as pruning and widening tree spacing enable to increase the total transmitted light within the
stand. On T. grandis, modification of the tree row azimuth resulted in changes in the spatial and seasonal distribution of light available for
the intercrops. These results are discussed in terms of agroforestry system management.
相似文献
Sylvie SabatierEmail: |
55.
Bananas cultivated for export all belong to Cavendish cultivars and are all recognized as very susceptible to nematodes, particularly
to the burrowing nematode Radopholus similis and the lesion nematode Pratylenchus coffeae. Even if there have been many changes in the management of banana nematodes in large commercial banana plantations, chemical
control still remains most often the last resort method to manage the nematodes, although the number of registered products
is definitely declining. Therefore, nematode control though genetic improvement is gaining new interest worldwide. In this
study, 55 banana accessions mostly diploids from the Musa acuminata genome group (AA) but including some triploid accessions (AAA), some diploids of the Musa balbisiana genome group (BB) and some interspecific hybrids (AAB, AB) were evaluated for resistance to four nematode species R. similis, P. coffeae, Meloidogyne incognita and M. arenaria. These experiments were conducted in a growth chamber under controlled conditions. All banana accessions were susceptible
to nematode species, although many different levels of susceptibility were detected. This study confirmed the good resistance
status to R. similis of some cultivars from the Pisang jari buaya and Pisang batuau subgroups and the partial resistance of 17 diploid accessions
significantly different from the susceptible reference cv. Grande Naine. This study also showed that 12 diploid accessions
exhibited a partial resistance to P. coffeae, including some usual or potential genitors belonging to the wild diploids subspecies burmannica (cvs. Long Tavoy 1 and 2) and burmannicoides (cv. Calcutta 4). No source of resistance to Meloidogyne spp. was found. These screening results, combining for the first time four nematode species, are discussed within the scope
of banana breeding in order to produce parental diploid lines with single or combined nematode resistances and further develop
triploids that can substitute existing susceptible commercial cultivars. 相似文献
56.
Annamaria Leccese Sylvie Bureau Maryse Reich M. G. C. Catherine Renard Jean-Marc Audergon Carmelo Mennone Susanna Bartolini Raffaella Viti 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(2):112-120
We have investigated the effect of cultivation systems and fruit post-harvest management on the antioxidant properties of
apricot fruits. Trees of five cultivars ‘Tyrinthos’, ‘Cafona’, ‘Bella d’Italia’, ‘Vitillo’ and ‘Pellecchiella’ were cultivated
under integrated and organic systems. Fruits were collected at full maturity stage and analyzed either immediately or after
storage at 4±0.5 °C and 85% of relative humidity for seven and 14 days. The main pomological traits (weight, colour, flesh
firmness, total soluble sugars, titratable acidity) and antioxidant properties were analyzed. The total antioxidant capacity
(TAC by TEAC method), total phenols content (TP by Folin-Ciocalteu method) and carotenoid content by HPLC-DAD were monitored.
Cultivar characterization by principal component analysis (PCA) indicated a large variability on pomological and antioxidant
properties of apricot fruits. ‘Bella d’Italia’ showed better TAC and TP values compared to the other cultivars. ANOVA interactions
between cultivar and cultivation system (organic/integrated) were found for the antioxidant properties. These interactions
may help to select a set of genotypes with better performances under organic system, which in our study might be indicated
in ‘Cafona’ and ‘Bella d’Italia’. 相似文献
57.
Behavioural responses to visual environment in early stage Atlantic cod Gadus morhua L. larvae
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Marine fish larvae exhibit species‐specific sensitivity to light, which changes during ontogeny. Our aim was to explore how visual environment, characterized by light intensity, microalgae addition and wall colour, affects spatial distribution of Atlantic cod larvae. We applied two different small‐scale factorial designs, each of them as a series of independent short‐term trials at fixed larval ages from 5 to 35 days post hatch (DPH). Positive phototaxis increased in larvae from 5 to ≈24 DPH, followed by reduced response at later ages. Effects from the experimental factors on larval behaviour changed with age, and significant interactions between factors were present. Algae significantly affected larvae only at 5 DPH, increasing phototactic response. White wall colour induced distribution of larvae close to the tank walls at all ages. The experimental approach demonstrated clear effects from visual environment on larval distribution, and revealed both relative importance of individual factors, interactive effects and temporal changes due to larval ontogeny. Our results suggest that by fine‐tuning the visual environment in tanks, the larval distribution may be managed to improve overall rearing conditions through a more even distribution with reduced closeness to tank walls. 相似文献
58.
Tonya A. Lander Etienne K. Klein Solenn Stoeckel Stéphanie Mariette Brigitte Musch Sylvie Oddou-Muratorio 《Landscape Ecology》2013,28(9):1769-1783
Widespread ecosystem change has led to declines in species world-wide. The loss of pollinators in particular constitutes a problem for ecosystem function and crop production. Understanding how landscape change affects pollinator movement, effective pollen flow, and plant and pollinator survival is therefore a global priority. In this study we investigated patterns of effective pollen flow, using wild cherry tree (Prunus avium) progeny arrays, to address two questions in three case studies: Do land-use types present different resistances to pollinator movement? Which pollinator travel path best explains the pollination data (straight lines, weighted straight lines, least cost paths or pair-wise resistance)? Trees and progeny arrays were genotyped and effective pollen flow and pollinator movement were estimated using the spatially explicit mating model. We found that pollinators did modify their travel paths in response to land-use type and arrangement, but the travel path that best described pollinator movement and the resistance rank of the land uses depended on the type and size of land-use patches and the landscape context. We propose a novel theoretical framework rooted in behavioural ecology, the resource model, for interpreting pollinator behaviour in heterogeneous landscapes. We conclude by discussing the importance and practicality of conservation and management strategies in which native and non-native land-use types together provide functional habitat and support ecosystem services across economic landscapes. 相似文献
59.
Bouhrara M Clerjon S Damez JL Kondjoyan A Bonny JM 《Journal of agricultural and food chemistry》2012,60(18):4678-4687
Understanding and monitoring deformation and water content changes in meat during cooking is of prime importance. We show the possibilities offered by nuclear magnetic resonance imaging (MRI) for the in situ dynamic measurement of deformation fields and water content mapping during beef heating from 20 to 75 °C. MRIs were acquired during heating, and image registration was used to calculate the deformation field. The temperature distribution in the sample was simulated numerically to link structural modifications and water transfer to temperature values. During heating, proton density decreases because of a magnetic susceptibility drop with temperature and water expulsion due to muscle contraction. A positive relationship was found between local cumulative deformation and water content. This new approach makes it possible to identify the deformation field and water transfer simultaneously and to trace thermal history to build heuristic models linking these parameters. 相似文献
60.
Summary TwoPythium spp. were isolated from diseased tubers exhibiting rots at harvest, in summer stores and also in refrigerated storage. Symptoms
shared features described for both pink rot caused byPhytophthora erythroseptica and leak caused byPythium spp.
The causal agents were identified asPythium aphanidermatum andP. ultimum by morphological and physiological observations and by fingerprinting using oomycete specific primers to amplify the internal
transcribed spacer (ITS1) within ribosomal DNAs. The optimum temperatures for infection were 30°C forP. aphanidermatum and 25°C forP. ultimum, corresponding to the optimum temperatures for growth in vitro. The optimum concentration for infection of tubers inoculated
by dipping in a suspension of oospores or hyphal swellings after wounding was found to be 103 reproductive organs/ml, whereas the infection threshold was 10 reproductive organs/ml. 相似文献