Supplemental biotin for swine. I. Influence on feedlot performance, plasma biotin and toe lesions in developing gilts

Three trials, utilizing a total of 240 crossbred gilts, were conducted to study the influence of 0 (NB) or 220 (SB) microgram supplemental biotin/kg of diet on feedlot performance, plasma biotin and development of toe lesions in developing gilts. Corn-soybean meal diets were fed from weaning to 92 kg body weight. Gilts were housed on expanded-metal floors to 50 kg body weight and on partially slatted concrete floors until completion of the trials. Feedlot performance, hair and structural soundness scores were not different (P greater than .10) between NB and SB gilts. Plasma biotin (PB) levels were elevated (P less than .01) when supplemental biotin was included in the diet. Gilts consuming SB diets had fewer (P less than .01) toe lesions/gilt and lower (P less than .01) toe lesion severity scores/gilt compared with gilts fed NB diets. Heel-horn erosion and heel cracks were the most frequent toe lesions observed. Fewer gilts (P less than .01) developed side-wall toe cracks when fed SB diets. Supplemental biotin reduced the frequency of individual toes containing heel-horn erosion (P less than .10), heel cracks (P less than .05) and side-wall toe cracks (P less than .05) with the severity of these lesions not affected (P greater than .10) by supplemental biotin. These results suggest that biotin levels in corn-soybean meal diets are adequate for feedlot performance, and that supplemental biotin can improve hoof integrity, but will not prevent toe lesions.     

Amitotic neuroblastoma cells used for neural implants in monkeys

The potential utility of cultured neuroblastoma cells as donor tissue for neutral implants into the mammalian brain has been examined. Cells from a human neuroblastoma cell line, IMR-32, were labeled with [3H]thymidine and chemically rendered amitotic. These differentiated IMR-32 cells were grafted into the hippocampi of five adult African Green monkeys, and graft survival was evaluated for up to 270 days after transplantation. Autoradiographically labeled grafted cells were identified in four animals. Processes from grafted cells could be followed for distances of up to 150 micrometers into the host brain. No evidence for neoplastic growth of the transplant was found. Thus, grafted neuroblastoma cells can survive for prolonged periods in the primate brain and may serve as a practical source of donor tissue for neural implants.     

Incidence and control of subclinical mastitis in intensively managed ewes

Ninety-three intensively managed, mature ewes were followed through three lactations to study effects and interrelationships of postweaning fast, prophylactic antibiotic injection, number of lactations and breed on subclinical mastitis, milk production and milk composition. Animals used in a 2 x 2 factorial design were allotted to treatment combinations of 1) postweaning 72 h fast or no fast, and 2) 1 x 10(6) units of procaine penicillin or sham i.m. injection at weaning. Treatments were implemented at weaning in yr 1; each ewe received the same treatment in each of the subsequent 2 yr of the study. Breeds included Finnish Landrace (F), Dorset (D), Rambouillet (R), Finnish Landrace x Dorset (FD) and Barbados Blackbelly x Dorset (BD). Milk production was greatest for D, followed by BD, FD, R and F during yr 1 (P less than .01) and overall (P less than .05). Milk production data were not collected in yr 2, but similar results were obtained in yr 3, except that the R and F exchanged rank positions. Milk composition was affected by breed. Breeds differed (P less than .05) in percentage lactose, fat and total nonfat solids in yr 1 and differed (P less than .01) in percentage lactose, fat, solids and protein across the 2 yr. A breed difference (P less than .05) was observed for incidence of mastitis at 3 wk postpartum and at weaning. Milk production was negatively correlated with incidence of mastitis (r = -.22, P less than .05). Milk production was improved (P less than .05) by a 72-h postweaning fast.(ABSTRACT TRUNCATED AT 250 WORDS)     

Restricted energy intake and elevated calcium and phosphorus intake for boars during growth. II. Foot and leg measurements and toe and soundness scores

Boars were assigned at 5 wk of age in a 2 X 2 factorial arrangement to two energy (ad libitum and 75% of ad libitum) and two Ca and P levels (100 and 150% of the National Research Council daily recommended amounts) to determine the effect of reduced growth rate and elevated Ca and P intake on foot and leg measurements, pad and horn incidence and severity of lesions, and structural soundness. There were 20 boars/treatment; 15 boars from each treatment were necropsied at 10 +/- 2-d intervals from 80 to 220 d of age (45 to 185 d on test), with the remaining five boars in each treatment being necropsied at 220 d of age. Feet and leg measurements were taken and toe pads and horns were scored initially and after 78 and 131 d on when necropsied. Boars remaining after 130 d on test were scored for overall structural soundness. Toe size and circumference and length of limbs increased as boars grew, with larger values for ad libitum-fed compared with limit-fed boars when observed at a constant age. However, when these values were corrected for body weight, the effect of energy was generally removed and, in some cases, reversed. Limit-fed boars appeared to have fewer pad and horn lesions, but the effects were inconsistent. In general, Ca and P levels had little or no effect on toe and limb sizes and pad and horn lesion scores, whether or not the values were expressed on a constant age or corrected body weight basis. A comparison of inside and outside toes on the front and hind feet revealed that outside toes were larger than inside toes, with the magnitude of the difference much larger for the hind foot than for the front foot. The hind inside toes had fewer pad and horn lesions. Structural soundness scores were more desirable in ad libitum-fed compared with limit-fed boars; but, elevated Ca and P levels had no effect. Thus, these data do not support a hypothesis that structural soundness can be improved by reducing the growth rate of developing boars or by feeding an elevated level of Ca and P.     

Effects of ram preexposure and ram breed on fertility of ewes in summer breeding

Suffolk x Rambouillet ewes were used in two consecutive years to test the ability of Dorset and Suffolk yearling rams to stimulate ovulation and estrus in early summer (late June and July) breeding. After spring weaning about June 1, ewes were randomly divided into three groups and for 2 wk either were isolated from rams or were preexposed to either yearling Dorset or Suffolk rams by penning of rams in proximity to ewes. Ewes then were rerandomized into two groups for joining with either yearling Dorset or Suffolk rams fitted with marking harnesses. Serum progesterone profiles and crayon marks were used as indicators of ovulation and estrus. Overall lambing rate was not different among preexposure treatments for the 2 yr. However, early lambing rate (in the first 14 d of lambing season) was significantly affected by preexposure group (24% for Dorset vs 9% for Suffolk and 10% for isolation), especially in the 2nd yr (31% for Dorset vs 8% for both Suffolk and isolation). Ewes preexposed to Dorsets also lambed 10 d earlier (P less than .05) than ewes preexposed to Suffolks in yr 2, but the groups did not differ in yr 1. Ewes exposed to Dorset rams for breeding had higher overall lambing rates than ewes bred to Suffolks (75% vs 54%), especially in the 1st yr (77% vs 48%). Lambs sired by Dorset rams were born 6 d earlier in yr 1 (P less than .05) but only 1 d earlier in yr 2. Data from both years indicate that Dorsets are superior to Suffolks in ability to sire lambs in fall lambing systems.     

Sire breed effects in matings with Angus cows: I. Fertility, calf survival and performance to 18 months

Over six calf crops, 524 exposures of Angus cows to three sire breeds resulted in 432 weaned calves for an 82.4% calf crop. Least-squares means for weaning rate by sire breed were 81.8, 80.5 and 83.9% for Angus, Charolais and Holsteins, respectively. Sire breed effect was not significant for calving rate, calf mortality or overall weaning rate, but was significant for all growth traits from birth to 18 mo. Angus-sired calves were smallest at birth, grew at a slower rate and were lightest in weight at all ages. Charolais-sired calves were heaviest at all weigh periods and Holstein-sired calves were intermediate. Holstein-sired calves scored significantly lower for both grade and condition than did Angus- and Charolais-sired calves. Charolais-sired calves had the highest grade. Calving rate tended to be influenced by the effect of cow (P less than .10) and sire breed X year (P less than .001). Calving rate was highest for 3-yr-old and for 6- to 10-yr-old cows and was also 1.5 percentage units higher for crossbred matings. Cow productivity (kg of calf weaned/cow exposed) is a combined measure of fertility, calf survival and weaning weight. Although sire breed did not significantly affect cow productivity (P less than .20), actual values favored cows producing crossbred calves by 14 and 9 kg for Charolais and Holstein crosses, respectively. Likewise, weight differences at 18 mo favored these crosses by 50 and 32 kg for heifers and 72 and 62 kg for steers. Among the two crossbred types, Charolais X Angus heifers were 28 kg heavier (P less than .05) than Holstein X Angus heifers at 18 mo but the 10 kg difference in steers was not significant. The fact that the large sire breeds produced calves with heavier weaning weights without demonstrating significant increases in overall productivity points out the importance of high weaning rates.     

A comparison of Dorset and Finnish Landrace crossbred ewes

Data from 644 exposures and 552 lambings were used to analyze reproductive traits of 1/2-Finnish Landrace, 1/2-Dorset ewes (1/2 Finn); 1/4-Finnish Landrace, 3/4-Dorset ewes, (1/4 Finn) and Dorset ewes. Ewes were exposed to Hampshire or Suffolk rams for spring lambing from 1973 to 1978. The analytical model included fixed effects of ewe birth year (EBY), ewe breed (BR), lambing year within EBY and EBY X BR interaction and random effects of ewe within EBY and BR. Conception rate differed (P less than .01) for 1/2-Finn (.94), 1/4-Finn (.88) and Dorset (.80) ewes. Litter size was highest (P less than .01) for 1/2-Finns (1.97), followed by 1/4-Finns (1.74) and Dorsets (1.44). The 1/2-Finn ewes ranked first for number and weight of lambs weaned, and for number and weight marketed as feeder lambs. Birth weights were heavier (P less than .001) for lambs with Dorset dams (3.88 kg) than for lambs with 1/4-Finn (3.24 kg) or 1/2-Finn (3.08 kg) dams. Preweaning average daily gains and adjusted 90-d weights were also larger (P less than .001) for lambs with Dorset dams (.26 kg/d and 28.3 kg, respectively) than for lambs with 1/4-Finn (.24 kg/d and 25.4 kg) or 1/2-Finn (.24 kg/d and 25.1 kg) dams. However, Dorset ewes lambed an average of 10.5 d later than Finn-cross ewes, and actual weaning weights (unadjusted for age) did not differ significantly among lambs from Dorset (27.2 kg), 1/4-Finn (26.9 kg) and 1/2-Finn ewes (26.7 kg).(ABSTRACT TRUNCATED AT 250 WORDS)     

The effect of fructose supplementation on feed intake,nutrient digestibility and digesta retention time in Reeves’s muntjac (Muntiacus reevesi)

The aim of the study was to determine the effect of fructose supplementation in the diet on feed intake, nutrient digestibility and digesta retention time in Reeves's muntjac (Muntiacus reevesi), a browsing cervid. In Experiment 1, six adult males of Reeves's muntjac were used in a replicated 3 × 3 Latin square design and fed a diet consisting of dehydrated chopped lucerne (ad libitum), high‐fibre pellet (120 g/day) and wheat bran (30 g/day) without (F0) or with addition of 12 and 24 g fructose/day (F12 and F24, respectively). In Experiment 2, the same six adult muntjacs were used in crossover design and fed F0 or F12. Doses of supplemental fructose were set to increase intake of water‐soluble carbohydrates (WSC; ≈40 g/day; ≈8% of WSC in consumed dry matter [DM]) by 25 and 50% relative to F0. Feed intake was controlled daily (Experiment 1 and 2) and total tract digestibility and digesta retention time were determined (Experiment 2). In Experiment 1, DM intake of chopped dehydrated lucerne decreased with fructose supplementation (F0 vs. F12 and F24; p = .01) but was not different between F12 and F24 (p = .76). Total DM intake was also not different between treatments (p ≥ .13). In Experiment 2, DM intake of lucerne, total DM intake and nutrient digestibility was not affected by fructose supplementation (p ≥ .17), but mean retention time of long particles in the whole GIT tended to be longer for F12 compared to F0 (p = .09). Under conditions of the current study, additional fructose intake (resulting in a range of WSC content in consumed DM from 8.6% to 13%) had only minor impact on feed intake and investigated functions of the gastrointestinal tract of Reeves's muntjac.