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121.
The presence and vasoactive effects of native calcitonin gene-related peptide (CGRP), substance P (SP), and neurokinin A (NKA)
were studied on isolated small branches of the coeliac artery from Atlantic cod, Gadus morhua, using immunohistochemistry and myograph recordings, respectively. Immunohistochemistry revealed nerve fibers containing
CGRP- and SP/NKA-like material running along the wall of the arteries. CGRP induced vasorelaxation of precontracted arteries
with a pD2 value of 8.54 ± 0.17. Relaxation to CGRP (10−8 M) was unaffected by l-NAME (3 × 10−4 M) and indomethacin (10−6 M) suggesting no involvement of nitric oxide or prostaglandins in the CGRP-induced relaxation. SP and NKA (from 10−10 to 3 × 10−7 M) contracted the unstimulated arteries at concentrations from 10−8 M and above in 42% and 33%, respectively, of the vessels. It is concluded that the innervation of the cod celiac artery includes
nerves expressing CGRP-like and tachykinin-like material, and that a vasodilatory response to CGRP is highly conserved amongst
vertebrates while the response to tachykinins is more variable. 相似文献
122.
Somatic embryogenesis is the preferred method for cell-to-plant regeneration of grapevine. In this study, we tested the embryogenic capacity of anther-derived calli from 59 grape genotypes, representing a diverse group of Vitis vinifera and hybrid varieties, and hybrids and accessions of non-vinifera Vitis species. Most genotypes were tested on two types of media: MST1 medium, which contained plant growth regulators (PGRs) 2,4-dichlorophenoxyacetic acid (2,4-D) and thidiazuron (TDZ), and MSE medium, which contained 2,4-D and 6-benzylaminopurine (BAP). Twenty-four of the grape genotypes produced embryogenic callus on one or both of these media, eighteen of which have not been reported to form somatic embryos before. The results also suggested that the various PGR combinations are differentially effective at inducing somatic embryos in various classes of grape genotypes. For example, seven of the eight V. vinifera conv. occidentalis varieties brought forth somatic embryos on MSE medium, and three out of four American Vitis genotypes produced somatic embryos on MST1 medium. We could not observe any apparent association between frequency of callus formation and embryogenic capacity of the anthers. 相似文献
123.
Christiane Felfe Matthias Schemainda Susanne Baldermann Naoharu Watanabe Peter Fleischmann 《茶叶》2013,(4):308-316
Carotenoid cleavage enzymes isolated from Japanese Camellia sinensis leaves (cultivar Yabukita) were used for investigating the structural patterns of carotenoid cleavage enzymes.Fresh tea leaves were used for the isolation of active enzymes and purified to single band stage in SDS PAGE gels after isoelectric focusing.The specific activity of the carotenoid cleavage enzymes was tested and the active fractions selected for further analysis.The sugar content and the amount of phosphate present in the purified enzymes were elucidated by the following methods:Phosphates were detected by phosphatase assays,fluorescence marker kits and ammoniumheptamolybdate complex measurements after incineration of the samples.Sugars were detected in gels using PAS reagent (periodide acid/ Schiff reagent) staining and by GC-MS after hydrolysation of the proteins with trifluoric acid.Phosphorylations as well as glycosylations of the samples could be detected in all cases,thus giving evidence for an increasing phosphorylation level of proteins in Camellia sinensis from spring (1.84 g/mg) to autumn (2.39 g/rg) as well as the presence of at least four different sugars (arabinose,xylose,galactose and ribose).These secondary modifications of the carotenoid cleavage enzymes and their dependency on the harvesting season may well correspond to the changes on the functional level which were detected between spring (Michaelis Constant (Km) =9.45 mol/1) and autumn (K =17.16 mol/l) harvests. 相似文献
124.
Natalia Ladygina Frederic Henry Robert Koller Alia Rodriguez Ilja Sonnemann Susanne Wurst 《Soil biology & biochemistry》2010,42(12):2266-2275
The productivity and diversity of plant communities are affected by soil organisms such as arbuscular mycorrhizal fungi (AMF), root herbivores and decomposers. However, it is unknown how interactions between such functionally dissimilar soil organisms affect plant communities and whether the combined effects are additive or interactive. In a greenhouse experiment we investigated the individual and combined effects of AMF (five Glomus species), root herbivores (wireworms and nematodes) and decomposers (collembolans and enchytraeids) on the productivity and nutrient content of a model grassland plant community as well as on soil microbial biomass and community structure. The effects of the soil organisms on productivity (total plant biomass), total root biomass, grass and forb biomass, and nutrient uptake of the plant community were additive. AMF decreased, decomposers increased and root herbivores had no effect on productivity, but in combination the additive effects canceled each other out. AMF reduced total root biomass by 18%, but decomposers increased it by 25%, leading to no net effect on total root biomass in the combined treatments. Total shoot biomass was reduced by 14% by root herbivores and affected by an interaction between AMF and decomposers where decomposers had a positive impact on shoot growth only in presence of AMF. AMF increased the shoot biomass of forbs, but reduced the shoot biomass of grasses, while root herbivores only reduced the shoot biomass of grasses. Interactive effects of the soil organisms were detected on the shoot biomasses of Lotus corniculatus, Plantago lanceolata, and Agrostis capillaris. The C/N ratio of the plant community was affected by AMF.In soil, AMF promoted abundances of bacterial, actinomycete, saprophytic and AMF fatty acid markers. Decomposers alone decreased bacterial and actinomycete fatty acids abundances but when decomposers were interacting with herbivores those abundances were increased. Our results suggests that at higher resolutions, i.e. on the levels of individual plant species and the microbial community, interactive effects are common but do not affect the overall productivity and nutrient uptake of a grassland plant community, which is mainly affected by additive effects of functionally dissimilar soil organisms. 相似文献
125.
Kjell Nilsson Ulrika kerlund Cecil C. Konijnendijk Alexander Alekseev Ole H. Caspersen Susanne Guldager Evgeny Kuznetsov Alexander Mezenko Andrey Selikhovkin 《Urban Forestry & Urban Greening》2007,6(2):93-101
Recent years have seen the introduction of the concept of urban greening, defined as embracing the planning and management of all urban vegetation to create or add values to the local community. Green-space development has become recognised by international agencies and donors as important tool in improving the quality of urban livelihoods and urban environment. This paper evaluates an example of an urban greening aid project, carried out by Danish and Russian partners in the city of St. Petersburg, Russia. The project aimed to contribute to conservation and development of the cultural–historical, social and ecological values of St. Petersburg's urban green areas by implementing a structured, socially inclusive, well-informed planning and management approach. The project had three main components: (1) the development of a GIS-based information system to assist green-space planning and management; (2) on-site improvements in selected green areas and (3) awareness raising and public involvement activities. Ex post evaluation of the project showed that in spite of the limits of time and resources, important results were achieved. A more strategic approach to urban green-space planning and management, as promoted by urban greening, was adapted by some of the Russian project partners. Achievements also included notable physical improvements to one park. But the main project impacts were improved communication and collaboration between the local park department and local academia, as well as expertise developed in running a complex urban greening project. The project failed, however, in its public involvement ambitions.Moreover, the project should have facilitated discussion on some of the current premises of urban green-space planning and management in St. Petersburg, which insufficiently consider changing values and public preferences related to green spaces. 相似文献
126.
Zusammenfassung Brennkirschen sind Süßkirschensorten, welche spezielle Eigenschaften für die Branntweinproduktion und die industrielle Verarbeitung besitzen. Ihr Hauptanbaugebiet in Deutschland befindet sich in Baden-Württemberg und in Teilen Bayerns. In den letzten Jahren wurde über einen geringen Fruchtbehang in neu gepflanzten Brennkirschenanlagen berichtet. Süßkirschen sind selbstinkompatibel und benötigen deshalb eine Bestäubersorte. Ursache ist ein gametophytischer Selbstinkompatibilitätsmechanismus, bedingt durch einen S-Lokus. Mit Hilfe molekularer Marker wurden deshalb Untersuchungen zu den S-Allel-Kombinationen in 62 Brennkirschensorten bzw. -herkünften aus dem Raum Oberkirch, Mittelbaden, durchgeführt. Im Ergebnis konnten zwei bisher noch nicht beschriebene S-Allele und 14 neue Inkompatibilitätsgruppen ermittelt werden. Ursache für den schlechten Fruchtansatz in neuen Kirschenanlagen mit den Hauptsorten Dolleseppler und Benjaminer sind die gleichen S-Allel-Kombinationen (S1S7) in beiden Sorten. Als mögliche Befruchtersorten werden die Sorten Schwarze Schüttler (S6S7), Hartwälder (S4S7), Feuerkirsche (S4S14) und die ehemalige Sorte Dolleseppler der Baumschule Kiefer (S1S4) empfohlen. 相似文献
127.
Rooftop gardens, rooftop greenhouses and indoor farms (defined as ZFarming) have been established or planned by activists and private companies in Berlin. These projects promise to produce a range of goods that could have positive impacts on the urban setting but also carry a number of risks and uncertainties. In this early innovation phase, the relevant stakeholders’ perceptions and social acceptance of ZFarming represent important preconditions for success or failure of the further diffusion of this practice. We used the framework of acceptance to investigate the stakeholders’ attitudes and to identify the key factors that might hinder or promote the introduction of ZFarming. The results are based on an analysis of 38 qualitative interviews conducted with key stakeholders in Berlin. As the results show, major perceived benefits of ZFarming include improved consumer awareness, education, and the creation of experimental spaces. Stakeholders further perceive opportunities for resource savings, new business models, repurposing of abandoned buildings and improved aesthetics. Major perceived risks are associated with growing techniques that are considered “unnatural”, health risks (due to urban pollutants), conflicts with images of traditional agriculture, the rejection of animal production in urban areas, the risk of projects being too expensive and too complex or being implemented too early, i.e. before the mechanisms are fully understood. The analysis further reveals which contextual factors—political, legal, market-related, spatial or societal—might negatively or positively influence ZFarming acceptance. 相似文献
128.
129.
Regine Brandt Susanne Lachmuth Catharina Landschulz Friedemann Haß Isabell Hensen 《New Forests》2014,45(6):777-795
Integrating native trees in farmland can support soil, water and biodiversity conservation. This is particularly important in regions characterized by long periods of drought and soil erosion, such as the Bolivian Andes, where agroforestry with native woody species is rarely applied. Better knowledge on the effects of environmental stress on propagation and establishment of such native plants is needed to optimize their cultivation. In our study, we tested the effects of temperature and scarification on seed germination, and assessed seedling survival and juvenile growth of two potential agroforestry species (Prosopis laevigata var. andicola, Schinus molle) under diverse soil and water conditions. Temperatures above 30 °C accelerated germination, but they increased fungi infestation in the case of S. molle. The application of acid and mechanical scarification significantly improved the germination capacity of P. laevigata var. andicola. Medium to high moisture levels in sand provided the most favourable conditions for plant growth. S. molle was more sensitive to dry and P. laevigata var. andicola more vulnerable to water-saturated clay loam. Mulching enhanced the survival and growth of S. molle juveniles, but increased P. laevigata var. andicola’s growth in sand and dry soils only. Our results may facilitate guidance on improving propagation of these two potential agroforestry species under environmental stress conditions. More generally, our study shows that easily applicable treatments, such as mulching, can significantly improve the cultivation of native species, provided that their habitat requirements and limiting factors are well known. This highlights the relevance of identifying and closing such knowledge gaps for native trees and shrubs in order to promote their potential for use in agroforestry. 相似文献
130.
Dong C Flecks S Unversucht S Haupt C van Pée KH Naismith JH 《Science (New York, N.Y.)》2005,309(5744):2216-2219
Chlorinated natural products include vancomycin and cryptophycin A. Their biosynthesis involves regioselective chlorination by flavin-dependent halogenases. We report the structural characterization of tryptophan 7-halogenase (PrnA), which regioselectively chlorinates tryptophan. Tryptophan and flavin adenine dinucleotide (FAD) are separated by a 10 angstrom-long tunnel and bound by distinct enzyme modules. The FAD module is conserved in halogenases and is related to flavin-dependent monooxygenases. On the basis of biochemical studies, crystal structures, and by analogy with monooxygenases, we predict that FADH2 reacts with O2 to make peroxyflavin, which is decomposed by Cl-. The resulting HOCl is guided through the tunnel to tryptophan, where it is activated to participate in electrophilic aromatic substitution. 相似文献