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61.
Water-soluble chitin (WSC) was prepared by carefully deacetylating chitins to about 50% of N-acetyl content. Topical formulations based on WSC were prepared and their effects on wound healing were evaluated on a rabbit ear model. Full-thickness, open skin wounds were made on the ears of rabbits and WSC ointments were embedded in the open wounds. The application of WSC ointments significantly accelerated wound healing and wound contraction. The areas of epithelialization and granulation tissues in WSC ointment group are remarkably larger than those in control group (no treatment) and in placebo group (treated with ointment-base materials). A large number of grown granulation tissues including dense fibroblast deposition were observed under the thickened epithelium of the wound treated with WSC ointments. The number of inflammatory cells in WSC ointment group was significantly decreased compared with those in control and placebo groups, indicating that WSC would give low stimuli to wounds and prevent excessive scar formation. Neovascularization was the most prominent in WSC ointment group. Wound contraction in WSC ointment group was much larger than those in control and placebo groups. Overall results demonstrate that the topical formulation based on WSC is considered to become an excellent dressing as a wound healing assistant.  相似文献   
62.
The structural feature of macromolecular component of dhaincha, cotton stalks, jute fiber, rice straw and wheat straw, which are commonly used in paper pulp production in forest deficient countries, was thoroughly studied. Lignin was isolated by classical Bjorkman method and characterized by elemental and methoxyl analysis, alkaline nitrobenzene oxidation, FTIR and 1H NMR spectroscopy. The C9 formulas for cotton stalks, jute fiber, dhaincha, rice straw and wheat straw were C9H8.95O3.53(OCH3)1.00, C9H8.12O4.03(OCH3)1.65, C9H8.10O4.65(OCH3)1.32, C9H8.58O3.74(OCH3)1.23 and C9H8.31O3.54(OCH3)1.23, respectively. The alkaline nitrobenzene oxidation products showed that syringyl to vanilin ratio of these nonwood varied from 1.1 to 2.9. Jute fiber showed the highest syringyl to vanilin ratio that are consistent with C9 formula. The β-O-4 units in these nonwood lignins had predominately erythro stereochemistry type. The crystalline structure of these nonwood cellulose was also studied using X-ray diffraction and FTIR spectroscopy. The proportions of crystallinity, crystal size were varied from plant to plant. Jute fiber showed the highest proportion of crystallinity (73.4%) and crystal size (4.2 nm). The degree of polymerization of these nonwoods cellulose has also been studied. Degree of polymerization of jute cellulose was also the highest (3875). FTIR spectroscopy showed that these nonwoods cellulose was monoclinic unit cell structure (Iβ). Carbohydrate analysis showed that the main sugar component in the hydrolyzates of these nonwoods were xylose apart from glucose.  相似文献   
63.
A new bioactive sterol glycoside, 3β-O-(3,4-di-O-acetyl-β-d-arabinopyranosyl) -25ξ-cholestane-3β,5α,6β,26-tetrol-26-acetate) (carijoside A, 1), was isolated from an octocoral identified as Carijoa sp. The structure of glycoside 1 was established by spectroscopic methods and by comparison with spectral data for the other known glycosides. Carijoside A (1) displayed significant inhibitory effects on superoxide anion generation and elastase release by human neutrophils and this compound exhibited moderate cytotoxicity toward DLD-1, P388D1, HL-60, and CCRF-CEM tumor cells.  相似文献   
64.
This study was performed to estimate the nitrite toxicity to southern flounder, Paralichthys lethostigma, in brackish water (7.5 ppt of salinity). For a LC50 test, 20 fingerlings (5.7 ± 0.4 cm) in each aquarium (15 L) were exposed to the concentrations of 0, 1, 5, 10, 15, 30, 60, 120, and 240 mg NO2?‐N/L in duplication for 10 d. Median lethal concentration at 96 h (96‐h LC50) was calculated as 81.6 mg NO2?‐N/L. For a verification test, young flounder (164.2 ± 9.1 g) were exposed to a simulated culture condition in recirculating systems (1000 L). Sodium nitrite was not added to control system, whereas it was added to Treatment system 1 (TS 1) and Treatment system 2 (TS 2) to maintain nitrite concentrations of 20 and 30 mg NO2?‐N/L, respectively. The plasma nitrite concentrations of the young flounder in TS 1 and TS 2 were 4.5 and 6.6 mg NO2?‐N/L, respectively, after 2 wk. At this time, the methemoglobin percentages in TS 1 and TS 2 reached 85.8 and 89.7%, and survival rates were 37.5 and 25.0%, respectively. The results of these tests indicate that southern flounder do not concentrate nitrite in blood from the environment, but they seem to be more sensitive to nitrite compared with other species that do not concentrate nitrite.  相似文献   
65.
Sponge-derived scalaranes are remarkable sesterterpenoids previously found to exhibit profound inhibitory effects against neutrophilic inflammation. In our current work, we constructed the metabolomic profile of marine sponge Lendenfeldia sp. for the first time using a tandem mass spectrometry (MS/MS) molecular networking approach. The results highlighted the rich chemical diversity of these scalaranes, motivating us to conduct further research to discover novel scalaranes targeting neutrophilic inflammation. MS- and NMR-assisted isolation and elucidation led to the discovery of seven new homoscalaranes, lendenfeldaranes K–Q (1–7), characterized by methylation at C-24, together with five known derivatives, lendenfeldarane B (8), 25-nor-24-methyl-12,24-dioxoscalar-16-en-22-oic acid (9), 24-methyl-12,24,25-trioxoscalar-16-en-22-oic acid (10), felixin B (11), and 23-hydroxy-20-methyldeoxoscalarin (12). Scalaranes 1–4 and 6–12 were assayed against superoxide anion generation and elastase release, which represented the neutrophilic inflammatory responses of respiratory burst and degranulation, respectively. The results indicated that 1–3 and 6–12 exhibited potential anti-inflammatory activities (IC50 for superoxide anion scavenging: 0.87~6.57 μM; IC50 for elastase release: 1.12~6.97 μM).  相似文献   
66.
We evaluated the role of flatfishes in the organization and structure of the eastern Bering Sea ecosystem using the Ecopath/Ecosim approach. As basic input data for the Ecopath/Ecosim model, we used estimates of biomass from bottom trawl surveys and age-structured population models, production/biomass (P/B) ratio, consumption/biomass (Q/B) ratio, diet composition (DC), and fisheries harvests for each component of species or species groups. We estimated the trophic level of each component, niche overlaps among flatfishes, and the impacts of competition and predation on flatfish species in the eastern Bering Sea ecosystem. Based on those estimates, we developed the tropho-dynamic structure of the ecosystem, and the model was used to simulate ecological effects of fishery exploitation patterns. No single flatfish species appeared to have a profound and uniquely important role in the organization and structure of the ecosystem. Instead, the most important component among the guild of flatfish species appeared to be yellowfin sole Pleuronectes asper, which had greater biomass than other flatfish and a relatively diverse diet among the small flatfish species. Pacific halibut Hippoglossus stenolepis, Greenland turbot Reinhardtius hippoglossoides, and arrowtooth flounder Atheresthes stomias were important keystone predators in the eastern Bering Sea ecosystem together with some groups of marine mammals and sea birds. Intra flatfish complex cannibalism was not observed, however, substantial diet overlaps were common in the flatfish guild system.  相似文献   
67.
Toxoplasma gondii is a ubiquitous intracellular protozoan parasite transmitted by food. Concerning this parasite, there are few studies done in Morocco. In this study, 261 sera from sheep intended for consumption in Marrakech were subjected to the Toxoplasma ELISA based serology test for the detection of anti-T. gondii specific IgG confirming a past infection. Of the total tested 72 (27.6%) sera were positive for IgG. This result shows that the seroprevalence approaches the world average and is similar to what is found in other cities of Morocco. This has prompted us to investigate other animal species in the region in order to evaluate the degree of contamination by this parasite as well as the potential risk incurred on consumption of their meat.  相似文献   
68.
Increased metabolic burdens in breeding sows, which are induced by elevated systemic oxidative stress, could increase the need for nucleotides to repair lymphocyte DNA damage; however, de novo synthesis of nucleotides may be insufficient to cover this increased need. This study investigated the effects of dietary nucleotides on milk composition, oxidative stress status, and the reproductive and lactational performance of sows. Forty multiparous sows were assigned to 2 dietary treatments (Control group, and 1 g/kg Nucleotides group) based on a randomized complete block design using their BW at 85 d of gestation as a block. Sows from 2 groups were fed a restricted diet during gestation and ad libitum during lactation. The experiment lasted from 85 d of gestation to 21 d of lactation. The reproductive performance of sows and the growth performance of suckling piglets were measured. Oxidative stress parameters and milk components were also analysed. Data were analyzed using contrasts in the MIXED procedure of SAS. Sows in the Nucleotides group consumed more feed during the first week (P < 0.01) and from 1 to 21 d (P < 0.05) of lactation than those in Control group. Correspondingly, the litter weight gain of piglets showed a tendency to increase from cross-fostering to 9 d (P = 0.09) and from cross-fostering to 20 d (P = 0.10) in the Nucleotides group relative to the Control group. Additionally, the Nucleotides group was higher (P < 0.01) than the Control group in the concentrations of uridine 5''monophosphate, guanosine 5''monophosphate, inosine 5''monophosphate, adenosine 5''monophosphate and total nucleotides in milk. Furthermore, the Nucleotides group was higher (P < 0.01) than the Control group in the serum levels of total antioxidant capacity (P < 0.01) for sows at 109 d of gestation and glutathione peroxidase for weaning piglets, but lower at the levels of thiobarbituric acid-reactive substances (P < 0.05) in serum of weaning piglets. This study indicated that maternal dietary nucleotides could promote piglet growth, probably due to the higher lactational feed intake and higher concentration of nucleotides in the milk of sows, and lower oxidative stress for both sows and piglets.  相似文献   
69.
We describe the development of solar water-splitting cells comprising earth-abundant elements that operate in near-neutral pH conditions, both with and without connecting wires. The cells consist of a triple junction, amorphous silicon photovoltaic interfaced to hydrogen- and oxygen-evolving catalysts made from an alloy of earth-abundant metals and a cobalt|borate catalyst, respectively. The devices described here carry out the solar-driven water-splitting reaction at efficiencies of 4.7% for a wired configuration and 2.5% for a wireless configuration when illuminated with 1 sun (100 milliwatts per square centimeter) of air mass 1.5 simulated sunlight. Fuel-forming catalysts interfaced with light-harvesting semiconductors afford a pathway to direct solar-to-fuels conversion that captures many of the basic functional elements of a leaf.  相似文献   
70.
Somatic cell nuclear transfer (SCNT) technology has recently been used to generate animals with a common genetic composition. In this study, we report the derivation of a pluripotent embryonic stem (ES) cell line (SCNT-hES-1) from a cloned human blastocyst. The SCNT-hES-1 cells displayed typical ES cell morphology and cell surface markers and were capable of differentiating into embryoid bodies in vitro and of forming teratomas in vivo containing cell derivatives from all three embryonic germ layers in severe combined immunodeficient mice. After continuous proliferation for more than 70 passages, SCNT-hES-1 cells maintained normal karyotypes and were genetically identical to the somatic nuclear donor cells. Although we cannot completely exclude the possibility that the cells had a parthenogenetic origin, imprinting analyses support a SCNT origin of the derived human ES cells.  相似文献   
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