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81.
Primary isolation and identification of avian rotaviruses from turkeys exhibiting signs of clinical enteritis in a continuous MA 104 cell line 总被引:4,自引:0,他引:4
Avian rotaviruses were isolated from turkeys with enteritis using MA 104 cell line. MA 104 cells were suitable for primary isolation and propagation of avian rotaviruses. Trypsin appeared essential for the enhancement of infectivity and the occurrence of cytopathic effect (CPE). Serum neutralization (SN), electron microscopy (EM), and analysis of genomic RNA were done to identify and confirm the identity of rotaviruses. Electrophoretic migration patterns of genomic RNA from avian rotaviruses were examined, and they were compared with those from mammalian rotaviruses. The migration patterns differed between these groups. 相似文献
82.
Ah Young Kim Hye-Sun Kim Ji-Houn Kang Mhan-Pyo Yang 《Journal of veterinary science (Suw?n-si, Korea)》2015,16(3):333-340
This study was conducted to determine whether serum adipokine concentrations differed between healthy dogs and dogs with diabetes mellitus (DM). To accomplish this, 19 dogs with newly diagnosed DM were compared to 20 otherwise healthy dogs. The serum concentrations of visfatin, leptin, IL-1β, IL-6, IL-18, and TNF-α were significantly higher in diabetic dogs than in healthy dogs, whereas the serum adiponectin concentrations were lower in diabetic dogs. However, there were no significant differences in the IL-10 and resistin levels between groups. The serum leptin concentrations in diabetic dogs with and without concurrent disorders differed significantly. Treatment with insulin induced a significant decrease in IL-6 in diabetic dogs without concurrent disorders. These results show that the clinical diabetic state of dogs could modulate the circulating visfatin and adiponectin concentrations directly, while upregulation of leptin was probably a result of concurrent disorders rather than an effect of persistent hyperglycemia as a result of DM. 相似文献
83.
Liya Bai Shuxia Gao Haitao Sun Xueyan Zhao Liping Yang Hongmei Hu Jie Sun Wenxue Jiang 《Journal of animal physiology and animal nutrition》2019,103(5):1602-1609
Wnt10b is a member of Wnt family that plays a variety of roles in biological functions, including those in the development of hair follicles. To investigate the effect of Wnt10b on hair growth in the Angora rabbit and to determine the underlying molecular mechanism, we cultured dermal papilla (DP) cells with exogenous Wnt10b in vitro. We observed the expressions of downstream critical gene β‐catenin and lymphoid enhancer‐binding factor 1 (LEF1) in Wnt/β‐catenin pathway. The levels of β‐catenin mRNA and protein were higher in the Wnt10b group of DP cells than in the Control group, and the mRNA level of LEF1 in the Wnt10b group was higher than in the Control group. Moreover, translocation of β‐catenin from cytoplasm to nucleus was activated in the Wnt10b group. Furthermore, the mRNA levels of the hair follicle‐regulatory genes, insulin‐like growth factor‐1 (IGF‐1) and alkaline phosphatase (ALP), and the protein activity of ALP was also upregulated in the Wnt10b group compared to their corresponding levels in the Control group. These data suggest that Wnt10b could activate the canonical Wnt/β‐catenin signalling pathway to induce DP cells in the Angora rabbit. In addition, the proliferation of DP cells was significantly promoted when cultured with Wnt10b for 48 and 72 hr, suggesting that Wnt10b plays a pivotal role in the proliferation and maintenance of DP cells in vitro. In conclusion, this study demonstrates that Wnt10b may promote hair follicle growth in Angora rabbit through the canonical Wnt/β‐catenin signalling pathway that promotes the proliferation of DP cells. 相似文献
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86.
不同遮荫强度对草地早熟禾草坪质量的影响 总被引:1,自引:0,他引:1
为研究不同遮荫强度对新建草地早熟禾(Poa pratensis)草坪生长状况的影响,于2009年5月1日-11月6日进行试验,共设计自然光照、轻度遮荫、中度遮荫和重度遮荫4种不同遮荫强度,对干旱胁迫下草地早熟禾的生长速率、草坪质量、草屑重量、叶色值、电导率和地下生物量等形态指标和生理指标进行观察测定。结果表明,在少雨时节中度遮荫的草坪质量较高,多雨时节则轻度遮荫的草坪质量较好;适度遮荫下草坪草的生长正常,叶色较好,电导率较小,草坪质量较高。因此,可以利用适度遮荫,减少夏季高温与干旱对草地早熟禾草坪的伤害,改善草坪质量。 相似文献
87.
规模养猪引起环境污染的防制对策 总被引:1,自引:0,他引:1
规模养猪场对外界环境污染物主要是粪尿污物及其产生的有害气体,为减少它们对周围环境的水、土壤、空气造成污染的程度,避免成为畜禽传染病、寄生虫病和人畜共患疾病的传染源,养猪场应采取一切行之有效的措施,加强和实施对生产污物的治理措施,在提高产量的同时,注重对环境的保护,减轻畜牧业生产活动对环境的危害,以促进养猪业经济效益、社会效益、生态效益的协调发展。 相似文献
88.
试验采用1日龄艾维菌肉仔鸡198只,随机分为6组,每组33只。核黄素添加量分别为0、3.6(1NRC)、7.2(2NRC)、14.4(4NRC)、21.6(6NRC)和28.8mg/kg(8NRC),其中0为对照组,5组为试验组,试验选在夏季高温季节进行(7~8月),试验期间鸡舍温度30—38℃,湿度60%-80%。试验鸡28日龄时,从198只肉仔鸡中每组选出体质量相近的肉仔鸡6只,共6组即36只,用全收粪法进行消化代谢试验,研究高温季节核黄素对日粮营养物质利用率的影响。研究结果表明,高温季节日粮中添加高水平的核黄素,与对照组相比提高肉仔鸡粗蛋白、粗脂肪和粗灰分的利用率。 相似文献
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90.
选用1日龄健康樱桃谷肉鸭新生雏144只,按体质量随机分为3组,每组设3个重复,每重复16只。对照组饲喂基础日粮,抗生素组添加5mg/kg黄霉素基础日粮,试验组1和组2在基础日粮中分别添加0.05%和0.10%合生素。结果表明,日粮中添加合生素对肉鸭日增质量、胸肌率和腿肌率有显著影响;对肉鸭的饲料转化率有极显著影响;对肉鸭的成活率、屠宰率和皮脂率无显著影响。与抗生素组相比,具有相似的作用。试验组1的日增质量比对照组提高8.56%,比试验组2提高5.54%,比抗生素组提高4.34%;试验组1的料肉比与对照组降低11.52%,比试验组2降低3.29%,比抗生素组降低3.24%。 相似文献