新城疫病毒La Sota株HN基因的克隆与序列分析

旨在从分子生物学角度进一步研究新城疫病毒La Sota株HN基因,探究NDV La Sota HN基因的遗传变异情况。研究以试验室冻存的p NDV-HN为模版,根据Gen Bank中登录的NDV La Sota株序列设计引物扩增HN基因,将其克隆到pMD18-T载体后进行鉴定,对鉴定正确的重组质粒p MD-NDV-HN进行测序分析。应用生物信息学软件对新城疫病毒Lasota株HN基因进行系统进化树分析、氨基酸序列同源性分析、糖基化位点、蛋白结构跨膜区分析及磷酸化位点预测分析。核苷酸序列测定结果表明:HN基因的序列长度为1743bp,该基因的开放阅读框(Open Reading Frame,ORF)总长为1716 bp,编码571个氨基酸。生物信息学表明:试验获得的HN基因具有6个潜在糖基化位点及12个半胱氨酸残基,第5个潜在糖基化位点(508-510 aa)发生缺失及第123位半胱氨酸残基被色氨酸残基取代。La Sota株HN基因编码的蛋白质中有29个丝氨酸、10个酪氨酸和16个苏氨酸可能成为蛋白激酶磷酸化位点。将试验获得的La Sota株HN基因序列和推导的氨基酸序列与新城疫毒株的HN基因相应序列比较后发现,它们的核苷酸序列同源性和氨基酸序列同源性最高均可达到99%,表明HN基因在种属间具有较高的保守性。研究为新城疫病毒的分子病毒学研究奠定了基础。     

农民生计多样性影响因素调查分析 ——以丰都县为例

丰都县农民生计多样性在整个西部地区比较具有代表性，但对于影响农民生计多样性的因素一直缺乏实证研究。该次调查采用参与性农村评估法（PRA），对丰都县名山镇（两汇口、古家店、鹿鸣寺）、社坛镇（三桥、德盛、干坛）、武平镇（殷家坝、坝周村）的农民生计方式及影响农民生计多样性的因素进行调查研究。研究表明，影响农民生计多样性的因素大致包括：自然资源、环境、人力资本、物质基础、社会关系、国家政策等方面     

The Shortcoming and Improvement of the Project Break-Even Analysis Method

This paper points out the shortcoming of the existing project break-even analysis method. A new improved method-the dynamic project break-even analysis method has been put forward.     

Controlling Techniques of Channel Sharing in HFC Network

The techniques of sharing a channel in HFC are original with regard to those of Ethernet and Ring. In this paper, the analysis is stressed on the method of the upstream bandwidth allocation, the collision resolving, and the relationship among them, beginning with MAP management message. The MAP is a complex frame, in which the messages of ranging opportunity needed by the CM at the access beginning, bandwidth allocation contention, bandwidth allocation and collision detection are included. The access way for a user is explained after the frame format and functions of a MAP are illustrated. Finally, upstream bandwidth allocation, the cause of collision, algorithm and the process to solve the collision and so on are studied.     

A Study on the Method of Time-Resource Optimization of Engineering Projects

This paper investigates the coordinated operation of the progress planning with the resource planning when carrying out engineering projects and puts forward a method of "RSM" under the resource limit conditions. Then, the "RSM" was improved to avoid complicated calculation. This method not only reduces the calculating work by hand but saves computer's memory as well, making the time-resource optimization work more quick and convenient.     

Unicity Theorem of Meromorphic Functions

ln this paper,we firstly in vestigated the un icity properties of meromorphic functions with three common values,extended the results of H. Ueda,Yi Hongxun.Moreover,wealso improved the results of K,Shibazaki,Yi Hongxun and C,C,Yang,Hua xinhou and some others     

Principle and Key Technology of Full Automatic Vicat Apparatus

In this paper the working principle and performance character of both the manual and automatic Vicat apparatuses in existence are compared and analyzed,and their existing weakness and the urgent problem to be solved are pointed out.On this basis,adopting the technology of mechatronics and modern sensors,a new sort of full automatic Vicat apparatus that can fully satisfy the national standard in testing the setting time of the cement has been developed.This apparatus can be operated expediently with high precision,the test results are to be analyzed and computed automatically.This paper presents the working principle,performance character,key technology and prospect of application for the full automatic Vicat apparatus.     

Calculation and Measurement of Electric Field along Polluted Cylindrical Insulator

In this paper,the electrical field distribution along the polluted cylindrical insu-lator before a nd after the local a re appea rs is calculated with the optim lzed pa rtially cha rge simula-tinn method and measured with the pockels device. Based on the calculating and measuring results,the in fluen ce of the electric field distri bu tion on the propagation of local a re in the flashover ofcyIlndrical insufator under various conditions is analyzed systematieally.     

对"影响大学生成才因素"的调查分析——以西南科技大学大学生为例

大学生是社会主义建设的栋梁,其思想观念、价值趋向以及日常行为都影响着其成才的趋向。在改革开放和建设有中国特色社会主义的伟大实践中,当代大学生的主流是好的,能够按照社会的需求来塑造自己。但是,随着社会改革的不断深入,大学生的思想观念、价值趋向以及日常行为都处在一种不断变化的过程之中,因此,提高我国大学生的综合素质,意义重大。     

板材多点成形工艺及装备

多点成形法是针对多品种、小批量的三维曲面板材成形的一种柔性加工法。采用这种方法可替代传统的模具,从而节省模具制造费用。本文介绍了有关这一加工方法的基本原理并在自制的简单多点试验机上进行了一系列试验,对4种不同的多点成形法的成形效果进行了讨论。