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171.
Three-dimensional (3D) movement of neuroprosthetic devices can be controlled by the activity of cortical neurons when appropriate algorithms are used to decode intended movement in real time. Previous studies assumed that neurons maintain fixed tuning properties, and the studies used subjects who were unaware of the movements predicted by their recorded units. In this study, subjects had real-time visual feedback of their brain-controlled trajectories. Cell tuning properties changed when used for brain-controlled movements. By using control algorithms that track these changes, subjects made long sequences of 3D movements using far fewer cortical units than expected. Daily practice improved movement accuracy and the directional tuning of these units. 相似文献
172.
Blackmore S 《Science (New York, N.Y.)》2002,298(5592):365
Taxonomy and systematics underpin our ability to conserve and benefit from biodiversity in sustainable ways as envisaged under the Convention on Biological Diversity (CBD). Despite progress in phylogenetics towards reconstructing the "Tree of Life" and in biodiversity informatics, the fundamental documentation of species necessary to complete the inventory of life has lagged behind. It is argued that this reflects a lack of appreciation of the role played by species-level taxonomic information in underpinning conservation and sustainable use and under investment in the relevant institutions at the expense of supporting the centralised financial mechanism of the CBD. 相似文献
173.
Bennett R 《Science (New York, N.Y.)》1947,106(2752):283-284
174.
Smith SM 《Science (New York, N.Y.)》2004,304(5678):1744; author reply 1744
175.
Bennett L 《Science (New York, N.Y.)》1966,152(3726):1263-1266
The source of vertical sustaining force for large insects capable of near-hovering flight is unclear. Induced velocities of a simulation of the insect Melolontha vulgaris in forward flight were measured with a hot-wire anemometer. The results were negative with respect to the drag concept of vertical force, but provided certain support for the circulation concept. Unsteady effects (the effects of variant air velocity) proved significant. 相似文献
176.
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178.
Silva JM Marran K Parker JS Silva J Golding M Schlabach MR Elledge SJ Hannon GJ Chang K 《Science (New York, N.Y.)》2008,319(5863):617-620
By virtue of their accumulated genetic alterations, tumor cells may acquire vulnerabilities that create opportunities for therapeutic intervention. We have devised a massively parallel strategy for screening short hairpin RNA (shRNA) collections for stable loss-of-function phenotypes. We assayed from 6000 to 20,000 shRNAs simultaneously to identify genes important for the proliferation and survival of five cell lines derived from human mammary tissue. Lethal shRNAs common to these cell lines targeted many known cell-cycle regulatory networks. Cell line-specific sensitivities to suppression of protein complexes and biological pathways also emerged, and these could be validated by RNA interference (RNAi) and pharmacologically. These studies establish a practical platform for genome-scale screening of complex phenotypes in mammalian cells and demonstrate that RNAi can be used to expose genotype-specific sensitivities. 相似文献
179.
Carbon tetrachloride at hepatotoxic levels blocks reversibly gap junctions between rat hepatocytes 总被引:2,自引:0,他引:2
Electrical coupling and dye coupling between pairs of rat hepatocytes were reversibly reduced by brief exposure to halogenated methanes (CBrCl3, CCl4, and CHCl3). The potency of different halomethanes in uncoupling hepatocytes was comparable to their hepatotoxicity in vivo, and the rank order was the same as that of their tendency to form free radicals. The effect of carbon tetrachloride (CCl4) on hepatocytes was substantially reduced by prior treatment with SKF 525A, an inhibitor of cytochrome P-450, and by exposure to the reducing reagent beta-mercaptoethanol. Halomethane uncoupling occurred with or without extracellular calcium and did not change intracellular concentrations of calcium and hydrogen ions or the phosphorylation state of the main gap-junctional protein. Thus the uncoupling appears to depend on cytochrome P-450 oxidative metabolism in which free radicals are generated and may result from oxidation of the gap-junctional protein or of a regulatory molecule that leads to closure of gap-junctional channels. Decreases in junctional conductance may be a rapid cellular response to injury that protects healthy cells by uncoupling them from unhealthy ones. 相似文献
180.
Wet weight of rat cerebral cortex was increased by exposure to an enriched environment, as compared with standard colony or impoverished conditions. Dry weights and wet weights were compared and both yielded identical percentage differences between brains of animals experiencing enrichment and those experiencing impoverishment. 相似文献