Improved sample preparation to determine acrylamide in difficult matrixes such as chocolate powder, cocoa, and coffee by liquid chromatography tandem mass spectroscopy

An improved sample preparation (extraction and cleanup) is presented that enables the quantification of low levels of acrylamide in difficult matrixes, including soluble chocolate powder, cocoa, coffee, and coffee surrogate. Final analysis is done by isotope-dilution liquid chromatography-electrospray ionization tandem mass spectrometry (LC-MS/MS) using d3-acrylamide as internal standard. Sample pretreatment essentially encompasses (a) protein precipitation with Carrez I and II solutions, (b) extraction of the analyte into ethyl acetate, and (c) solid-phase extraction on a Multimode cartridge. The stability of acrylamide in final extracts and in certain commercial foods and beverages is also reported. This approach provided good performance in terms of linearity, accuracy and precision. Full validation was conducted in soluble chocolate powder, achieving a decision limit (CCalpha) and detection capability (CCbeta) of 9.2 and 12.5 microg/kg, respectively. The method was extended to the analysis of acrylamide in various foodstuffs such as mashed potatoes, crisp bread, and butter biscuit and cookies. Furthermore, the accuracy of the method is demonstrated by the results obtained in three inter-laboratory proficiency tests.     

Interactions of formal and informal knowledge systems in village-based tree management in central India

This study critiques the idea of a Western science -- indigenous knowledge dichotomy in agricultural knowledge by examining the hybrid nature of knowledge use and incorporation by villagers in Madhya Pradesh, India. By analyzing knowledge systems as multi-leveled structures consisting of concrete practices linked to more abstract, explanatory concepts, this paper illustrates how information from multiple sources is integrated into local bodies of knowledge about tree management. Practices such as urea fertilization from formal global science might be explained by concepts such as that of a hot/cold duality from informal folk science. Similarly, other pieces of knowledge stemming from diverse knowledge systems are shown to become mixed and matched on practical and conceptual levels. Additionally, several knowledge elements used locally appear to be held in common by many knowledge systems around the world, rendering the determination of their origins in one system or another nearly impossible. These observations lead to the conclusion that local knowledge systems of tree management are better characterized as open systems rather than distinct, closed systems. Furthermore, the constant exchange of material between formal and informal, local and global systems renders untenable any strict dichotomy of knowledge systems.     

Carbon flux and growth in mature deciduous forest trees exposed to elevated CO2

Whether rising atmospheric carbon dioxide (CO2) concentrations will cause forests to grow faster and store more carbon is an open question. Using free air CO2 release in combination with a canopy crane, we found an immediate and sustained enhancement of carbon flux through 35-meter-tall temperate forest trees when exposed to elevated CO2. However, there was no overall stimulation in stem growth and leaf litter production after 4 years. Photosynthetic capacity was not reduced, leaf chemistry changes were minor, and tree species differed in their responses. Although growing vigorously, these trees did not accrete more biomass carbon in stems in response to elevated CO2, thus challenging projections of growth responses derived from tests with smaller trees.     

Reconstructing Farallon plate subduction beneath North America back to the Late Cretaceous

Using an inverse mantle convection model that assimilates seismic structure and plate motions, we reconstruct Farallon plate subduction back to 100 million years ago. Models consistent with stratigraphy constrain the depth dependence of mantle viscosity and buoyancy, requiring that the Farallon slab was flat lying in the Late Cretaceous, consistent with geological reconstructions. The simulation predicts that an extensive zone of shallow-dipping subduction extended beyond the flat-lying slab farther east and north by up to 1000 kilometers. The limited region of flat subduction is consistent with the notion that subduction of an oceanic plateau caused the slab to flatten. The results imply that seismic images of the current mantle provide more constraints on past tectonic events than previously recognized.     

Spatial variability of O layer thickness and humus forms under different pine beech–forest transformation stages in NE Germany

Spatial variability of humus layer (O layer) thicknesses can have important impacts upon soil water dynamics, nutrient storage and availability, as well as plant growth. The purpose of the present study was to elucidate the impact of forest‐transformation practices on the spatial variability of O layer thicknesses. The study focused on the Kahlenberg forest area (NE Germany) with stands of Scots pine (Pinus sylvestris) and European beech (Fagus sylvatica) of different age structures that form a transformation chronosequence from pure Scots pine stands towards pure European beech stands. Topsoil profiles including both, the O layer and the uppermost humic mineral soil horizon were excavated at intervals of 0.4 m along 15–20 m long transects, and spatial variability of O layer thicknesses was quantified by variogram analysis. The correlation lengths of total O layer thickness increased in the sequence consisting of pure pine stand (3.1 m) → older mixed stand (3.7 m) → pure beech stand (4.5 m), with the exception of the younger mixed stand, for which no correlation lengths of total O layer thickness could be determined. The degree of spatial correlation, i.e., the percentage of the total variance which can be described by variograms, was highest for the two monospecies stands, whereas this percentage was distinctly lower for the two mixed stands. A similar minimum for the two mixed stands was observed for the correlation lengths of the Oh horizon. These results suggest that the spatial structures of forest‐transformation stands may be interpreted in terms of a disturbance (in the form of the underplanting of beech trees). After this disturbance, the forest ecosystem requires at least 100 y to again reach relative equilibrium. These findings are in line with the results of other soil‐related investigations at these sites.     

Contamination of bela krajina,Slovenia with polychlorinated biphenyls 1. Levels of some high molecular chlorinated hydrocarbons in the water and fish of the Kupa River in Croatia

It is well documented that synthetic chlorinated hydrocarbon residues are widespread throughout the world ecosystem. Recently serious contamination of the Kupa River in Slovenia resulting from improper disposal of the waste disharged by an electro-capacitor manufacturing plant was observed. The migration of the PCBs from the waste tip into the karst ground water and source water has been established. PCBs have been also introduced into the small Lahinja River a tributary of the Kupa. Consequently, these pollutants have been detected in the Kupa river. An investigation of the levels of chlorinated hydrocarbons in tap water, water and fish samples collected from 1985 to 1988 from the Kupa River in the region of Petrinja and Sisak was performed. This region is about 200 km downstream of the primary contamined area. Concentrations of chlorinated insecticides and polychlorinated biphenyls in tap water ranged from <0.2 to 5.0 ng dm^?3 for PCBs such as Aroclor 1254 and from <0.4 to 10.4 ng dm^?3 for PCBs such as Pyralene 1500. In the Kupa River, water samples were found with PCBs such as Aroclor 1254 from <1.5 to 5.0 and from <2.6 to 104 ng dm^?3 for PCBs such as Pyralene 1500. The level of the investigated chlorinated insecticides were under the sensitivity limits of 0.05 for dieldrin and 0.1 ng dm^?3 for DDTs. Concentrations of chlorinated insecticides and polychlorinated biphenyls in the fish samples were several orders of magnitude higher in comparison with the water samples and ranged from the sensitivity limit for dieldrin (<0.0001) to 0.0034 mg kg^?1; p,p'DDT from 0.0003 to 0.0076 mg kg^?1; p,p'DDE from 0.0004 to 0.1752 mg kg^?1; p,p'DDD from 0.0001 to 0.0201 mg kg^?1 wet weight. Concentrations of PCBs were significantly higher and ranged from 0.015 to 2.742 mg kg^?1 wet weight for Pyralene 1500 and from 0.090 to 1.590 mg kg^?1 for Aroclor 1254 +Aroclor 1260.     

Effect of CO2 enrichment on photosynthesis,growth and yield of tomato

Net photosynthesis of tomato plants was measured as CO₂ uptake in various light intensities, CO₂ concentrations, O₂ concentrations and temperatures during short-term experiments. Net photosynthesis increased significantly with increasing CO₂ concentration at all light intensities, even at the lowest one. The optimal temperature for photosynthesis increased with CO₂ enrichment. The changes in photosynthesis when the $\text{CO}{}_2\text{O}{}_2$ ratio was varied suggest that the effect of CO₂ enrichment is a result of a reduction in photorespiration.To determine whether the increase in photosynthesis caused by CO₂ enrichment would produce a greater yield, tomato plants were cultivated from seed to harvest in a tightly-closed greenhouse that was enriched with CO₂ continuously during the entire growing-period. The control greenhouse was ventilated by openings in the roof and door. The temperature of the 2 greenhouses was kept the same. Plants enriched with CO₂ showed a significant increase in fresh and dry weight and yield of tomatoes. The results are discussed in relation to earlier work in which CO₂ enrichment was discontinued when there was a need for ventilation.     

Investigation of domestic animals and pets as a reservoir for intimin- (eae) gene positive Escherichia coli types

Domestic animals belonging to seven different species (cattle, sheep, dogs, cats, pigs, chicken and goats) were investigated as natural reservoirs for attaching and effacing Escherichia coli (AEEC). For this, 2165 E. coli strains from faeces of 803 animals were examined for the presence of the intimin -(eae) gene as a characteristic of AEEC strains. Ten percent of the animals were found to excrete AEEC, most frequently found in sheep (19.2%) and pigs (17.6), followed by cattle (10.4%), dogs (7.2%), cats (6.5%) and poultry (2.3%). The 97 AEEC strains from animals were grouped into 44 serotypes. Only four E. coli serotypes (O2:H8, O26:[H11], O109:[H25] and O145:[H28] were found in more than one animal host species. AEEC O26:[H11] strains were most frequently isolated (13.4%) being present in cattle, poultry, pigs and sheep. A search for virulence markers associated with enterohemorrhagic E. coli (EHEC) revealed Shiga-toxin genes in three (3.1%) AEEC strains from sheep. Bundle forming pili genes as a trait of typical enteropathogenic E. coli (EPEC) were detected in four (4.1%) strains from dogs and cats. The remaining 90 AEEC strains were classified as atypical EPEC. Typing of intimin genes revealed intimin beta being present in 51.5% of the strains, followed by intimins theta (23.7%), epsilon (6.2%), kappa (5.2%), zeta (5.2%), alpha, eta and iota (each 1.0%). Our data indicate that domestic animals and pets constitute an important natural reservoir of AEEC strains, and some of these (O26:[H11], O103:H2, O128:H2, O145:[H28] and O177:[H11]) are known to occur as pathogens in humans.