Combined genomic-metabolomic approach for the differentiation of geographical origins of natural products: deer antlers as an example

The correct identification of the geographical origin of deer antlers is essential to quality control, as its positive physiological effects correlate with chemical components. In this study, we applied both genomics and metabolomics to the origin-identification of 101 samples from Canada, New Zealand, and Korea. The genomics identified deer species in each country but failed to categorize all the samples, due to the presence of identical species in different countries. For identical species, NMR-based metabolomics gave clean separations, compounds specific to each country were identified, and the validity was confirmed by prediction analysis. As the genomics provided unambiguous read-outs for different species, and the metabolomics cleanly distinguished among identical species from different countries, their combined use could be a robust method for origin-identification even in difficult cases. We believe the method to be generally applicable to many herbal medicinal products for which various species are grown internationally.     

Food grade fungal stress on germinating peanut seeds induced phytoalexins and enhanced polyphenolic antioxidants

The effects of food grade fungus Rhizopus oligosporus stress on phytochemicals and phytoalexins of germinating peanut seeds were investigated by comparing the metabolic profiles of ungerminated (UG), germinated (G), and germinated seeds under fungal stress (GS). Three types of peanut seeds with different skin color (red, reddish brown, and black) were compared in the process. The polyphenolic contents were analyzed and correlated with antioxidant capacity for specific free radicals including peroxyl radical ROO(?) (ORAC), hydroxyl radical HO(?) (HORAC), superoxide radical O(2)(?-) (SORAC), and DPPH radical. The polyphenolic fingerprints analyzed by HPLC and LC-MS(n) showed that phenolic acids (coumaric, sinapinic, and ferulic acids derivatives) were the major group of phenolic compounds in ungerminated seeds. G or GS increased the level of phenolic acids, phytoalexins, and antioxidant capacity values in reddish and red peanuts but not in black peanuts. From the LC-MS(n) spectral data, 45 compounds were identified tentatively in the germinated peanuts, including 14 coumaric acids, 3 ferulic acids, 4 sinapinic acids, 2 hydroxybenzoic acids, 1 caffeic acid, 2 flavonoids, and 19 stilbenoids derivatives. Reddish brown germinated peanuts produced the highest amount of phytoalexins after GS with 55 compounds detected. Forty-five of these compounds were suggested as stilbenoid phytoalexins derivatives. The high content of phytoalexins may enhance the bioactivity of peanut seeds as functional food ingredients.     

Effects of Loosely Bound EPS Release and Floc Reconstruction on Sludge Dewaterability

Water, Air, & Soil Pollution - Minimizing the risks associated with manure-borne pathogenic microorganisms requires an understanding of microbial survival under realistic field conditions. The...     

Effect of microfluidization on in vitro micellization and intestinal cell uptake of lutein from Chlorella vulgaris

Chlorella is a nutrient-rich microalga that contains protein, lipid, minerals, vitamins, and high levels of lutein. This study evaluated the bioavailability of lutein from Chlorella vulgaris using a coupled in vitro digestion and human intestinal Caco-2 cell model. Lutein bioaccessibility was low, and approximately 75% of total C. vulgaris lutein was not micellized during the digestion process but remained in the insoluble digestate. Microfluidization improved lutein micellization efficiency during C. vulgaris digestion. C. vulgaris was microfluidized at a pressure exceeding 10000 psi, and the cell surface disruption was visualized by scanning electron microscopy. The mean C. vulgaris particle size was reduced from 3.56 to 0.35 μm with the microfluidization treatment. C. vulgaris microfluidization at 20000 psi was three times more efficient for aqueous lutein micelles production as compared with untreated C. vulgaris, and the final lutein content accumulated by intestinal Caco-2 cells was also higher with microfluidization. C. vulgaris lutein stability was not affected by microfluidization. These results indicate that microfluidization may be useful for improving lutein bioaccessibility from C. vulgaris during food processing.     

基于模糊评价的可重构制造系统生产计划制订方法

为快速合理地确定可重构制造系统新构形的生产任务,提出了一种生产计划制订方法。首先建立适合可重构制造系统的产品评价指标体系,运用模糊数学的理论和方法,对市场上同时出现的多种新产品进行评价,以选出适合于制造系统新构形的生产对象。在此基础上,构建了可重构制造系统生产计划的目标规划模型,并采用序贯式算法进行求解,从而制订出可重构制造系统的最优生产计划。     

日本设施栽培土壤热水消毒技术的发展现状

简要说明了土壤物理消毒技术的基本方法和原理,着重介绍了日本土壤热水消毒法的发展过程,土壤热水消毒的主要设备、基本作业步骤、土壤热水消毒的功效。     

棉花剥壳机参数及性能的优化

在对现有棉花剥壳机改进的基础上,应用正交试验研究法对影响棉花剥壳机性能的关键参数-叶片倾角、主轴导程和主轴转速进行了优化研究,发现主轴导程为影响分离率的重要因素,叶片倾角和主轴转速为主要因素,并分析了各因素对棉花剥壳分离率的影响趋势,从而为进一步改进棉花剥壳机的参数、提高棉花剥壳机的性能提供依据.     

东港灌区运行状况综合评价研究

在灌区续建配套与节水改造规划工作实施的基础上，拟建了一套合理的评价指标体系，利用层次分析法（AHP）确定评价指标的权重，建立了模糊综合评判（FCE）数学模型，并运用该模型对东港市的三个灌区的运行状况进行综合评价，评价结果与实际情况吻合良好。     

客车车身CAN总线网络通讯协议的制定与实现

控制局域网CAN（Controller Area Network）是20世纪80年代初德国Bosch公司为解决众多的控制设备和测试仪器之间的数据交换而开发的一种串行数据通讯协议。本文介绍了一种客车车身CAN总线网络控制系统,通过研究CAN2.0B规范。制定了符合该系统的通讯协议,并阐述了通讯软件的设计方案。     

温室测控系统开关设备优化组合预测控制方法

考虑开关设备组合作用下温室测控系统的非线性动态特性,提出了结构简单、不需复杂数值计算的离散预测模型,在不小于最大时滞时域内设备组合不变情况下,对设备组合进行滚动优化预测控制,从而大大简化了温室测控系统预测控制算法的复杂性,缓解了测控系统分布大时滞问题。仿真分析和温度的均方差表明,对分布式、大时延温室测控系统的优化组合预测控制方法是有效的。