全文获取类型
收费全文 | 282篇 |
免费 | 3篇 |
国内免费 | 1篇 |
专业分类
林业 | 11篇 |
农学 | 6篇 |
基础科学 | 1篇 |
13篇 | |
综合类 | 11篇 |
农作物 | 11篇 |
水产渔业 | 42篇 |
畜牧兽医 | 162篇 |
植物保护 | 29篇 |
出版年
2023年 | 1篇 |
2022年 | 12篇 |
2021年 | 16篇 |
2020年 | 1篇 |
2019年 | 2篇 |
2017年 | 2篇 |
2016年 | 3篇 |
2015年 | 14篇 |
2014年 | 19篇 |
2013年 | 14篇 |
2012年 | 8篇 |
2011年 | 22篇 |
2010年 | 5篇 |
2009年 | 17篇 |
2008年 | 25篇 |
2007年 | 21篇 |
2006年 | 15篇 |
2005年 | 13篇 |
2004年 | 18篇 |
2003年 | 19篇 |
2002年 | 19篇 |
2001年 | 4篇 |
2000年 | 9篇 |
1999年 | 2篇 |
1996年 | 1篇 |
1993年 | 1篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1959年 | 1篇 |
排序方式: 共有286条查询结果,搜索用时 15 毫秒
71.
Yusuke TANAKA Yuka TAGAINO Shotaro NAKAGUN Takayuki MINESHIGE Kenichi WATANABE Hisashi INOKUMA Yoshiyasu KOBAYASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(12):1852
A male Holstein-Friesian calf was born with multiple, cauliflower-like, pale pink cutaneous masses on the head and limbs. On histopathological examination, the cutaneous masses were diagnosed as congenital cutaneous fibropapillomatosis. Those lesions involved focal proliferation of sebaceous gland in the dermis. There were no histological findings to suggest bovine papillomavirus infection, such as the presence of intranuclear inclusion bodies, large keratohyalin granules, and koilocytosis. Furthermore, papillomaviral antigens and DNA were not detected by immunohistochemistry and polymerase chain reaction, respectively. These results suggested that there was no association between these cutaneous lesions and bovine papillomavirus infection, and the lesions were considered as harmartomatous changes. 相似文献
72.
Differential Expression of Two Cytosolic Ascorbate Peroxidases and Two Superoxide Dismutase Genes in Response to Abiotic Stress in Rice 总被引:3,自引:0,他引:3
Shigeto MORITA Shinya NAKATANI Tomokazu KOSHIBA Takehiro MASUMURA Yasunari OGIHARA Kunisuke TANAKA 《水稻科学》2011,18(3):157-166
Superoxide dismutase (SOD) and ascorbate peroxidase (APX) play central roles in the pathway for scavenging reactive oxygen species in plants, thereby contributing to the tolerance against abiotic stress. Here we report the responses of cytosolic SOD (cSOD; sodCc1 and sodCc2) and cytosolic APX (cAPX; OsAPX1 and OsAPX2) genes to oxidative and abiotic stress in rice. RNA blot analyses revealed that methyl viologen treatment caused a more prominent induction of cAPXs compared with cSODs, and hydrogen peroxide t... 相似文献
73.
Maha Laksha Mudiyanselage Chandrika Dissanayake Rumi Kashima Shuhei Tanaka Shin-ichi Ito 《Journal of General Plant Pathology》2009,75(1):37-45
Thirty-two isolates of Fusarium species were obtained from wilted Welsh onion (Allium fistulosum) grown on nine farms from six regions in Japan and identified as F. oxysporum (18 isolates), F. verticillioides (7 isolates), and F. solani (7 isolates). The pathogenicity of 32 isolates was tested on five commercial cultivars of Welsh onion and two cultivars of
bulb onion in a seedling assay in a greenhouse. The Fusarium isolates varied in the degree of disease severity on the cultivars. Five F. oxysporum isolates (08, 15, 17, 22, and 30) had a higher virulence on the cultivars than the other isolates. The host range of these
five isolates was limited to Allium species. Molecular characterization of Fusarium isolates was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the
internal transcribed spacer (ITS) regions of ribosomal DNA. The 32 isolates were grouped into eight types (four types for
F. oxysporum, one for F. verticillioides, and three for F. solani). Restriction patterns of the ITS region were not related to pathogenicity. However, the haplotypes obtained with five enzymes
(RsaI, HinfI, HaeIII, ScrFI, and MspI) and the phylogenetic analysis permitted the discernment of the three Fusarium species. The PCR-RFLP analysis should provide a rapid, simple method for differentiating Fusaruim species isolated from wilted Welsh onion in Japan. 相似文献
74.
Wind‐induced stock variation of the neon flying squid (Ommastrephes bartramii) winter–spring cohort in the subtropical North Pacific Ocean 下载免费PDF全文
Haruka Nishikawa Takahiro Toyoda Shuhei Masuda Yoichi Ishikawa Yuji Sasaki Hiromichi Igarashi Mitsuo Sakai Masaki Seito Toshiyuki Awaji 《Fisheries Oceanography》2015,24(3):229-241
Our examination of the neon flying squid (Ommastrephes bartramii) winter–spring cohort catch per unit effort (CPUE, an index of stock) revealed significant positive correlations with the interannual variations of observed chlorophyll‐a (Chl‐a) concentration and autumn–winter mixed layer depth (MLD) in the winter–spring feeding grounds of paralarvae and juveniles (130–170°E, 20–27°N). These correlations suggest the importance of integrated bottom‐up effects by the autumn–winter MLD for the neon flying squid stocks. However, the influence of autumn–winter MLD interannual variation in the forage availability for paralarvae and juveniles, i.e., particulate organic matter and zooplankton, has still been unclear. In this study, we use the lower trophic ecosystem model NEMURO, which uses the physical environmental data from the ocean reanalysis dataset obtained by the four‐dimensional variational (4DVAR) data assimilation method. The model‐based investigation enables us to clarify how the autumn–winter MLD controls the particulate organic matter and zooplankton abundance in the feeding grounds. Further, our investigation of the autumn–winter MLD interannual variation demonstrates that the stronger autumn wind in the feeding grounds develops a deeper mixed layer. Therefore, the deep mixed layer entrains nutrient‐rich water and enhances photosynthesis, which results in good feeding conditions for paralarvae and juveniles. Our results underline that the wind system interannual variation has critical roles on the winter–spring cohort of the neon flying squid stock. 相似文献
75.
Tomomi TANAKA Satoshi OHKURA Yoshihiro WAKABAYASHI Takenobu KUROIWA Kiyosuke NAGAI Natsumi ENDO Akira TANAKA Hisanori MATSUI Masami KUSAKA Hiroaki OKAMURA 《The Journal of reproduction and development》2013,59(6):563-568
The aim of the present study was to determine if the estradiol-induced luteinizing
hormone (LH) surge is influenced by the constant exposure to TAK-683, an
investigational metastin/kisspeptin analog, that had been established to depress the
pulsatile gonadotropin-releasing hormone (GnRH) and LH secretion in goats.
Ovariectomized goats subcutaneously received TAK-683 (TAK-683 group, n=6) or vehicle
(control group, n=6) constantly via subcutaneous implantation of an osmotic pump.
Five days after the start of the treatment, estradiol was infused intravenously in
both groups to evaluate the effects on the LH surge. Blood samples were collected at
6-min intervals for 4 h prior to the initiation of either the TAK-683 treatment or
the estradiol infusion, to determine the profiles of pulsatile LH secretion. They
were also collected at 2-h intervals from –4 h to 32 h after the start of estradiol
infusion for analysis of LH surges. The frequency and mean concentrations of LH
pulses in the TAK-683 group were remarkably suppressed 5 days after the start of
TAK-683 treatment compared with those of the control group (P<0.05). On the other
hand, a clear LH surge was observed in all animals of both groups. There were no
significant differences in the LH concentrations for surge peak and the peak time of
the LH surge between the TAK-683 and control groups. These findings suggest that the
effects of continuous exposure to kisspeptin or its analog on the mechanism(s) that
regulates the pulsatile and surge mode secretion of GnRH/LH are different in
goats. 相似文献
76.
Yoshikazu TAKETA Yasuhiro NAGAI Hideki OGASAWARA Shinichiro HAYASHI Masato MIYAKE Sachi TANAKA Kouichi WATANABE Shyuichi OHWADA Hisashi ASO Takahiro YAMAGUCHI 《Animal Science Journal》2008,79(3):382-390
Myostatin (MSTN), known as growth and differentiation factor 8 (GDF-8), is a member of the transforming growth factor β (TGF-β) superfamily that negatively regulates skeletal muscle mass. Myostatin binds with high affinity to the receptor serine threonine kinase activin receptor type IIB (ActRIIB). Activins that also belong to the TGF-β superfamily, stimulate follicle-stimulating hormone production in gonadotrophs and suppress growth hormone and adrenocorticotropic hormone production in somatotrophs and corticotrophs, respectively. The aim of the present paper was therefore to clarify the endocrine action of MSTN in adenohypophysis. The present study details the expression and cellular localization of MSTN and ActRIIB in porcine anterior pituitary gland. The mRNA of MSTN and ActRIIB was consistently expressed in RT-PCR. Immunohistochemistry of MSTN and specific hormones showed that MSTN localized in thyrotrophs and gonadotrophs, in which most of the MSTN immunoreactive cells were identified as thyrotrophs. The immunostaining of ActRIIB was restricted to corticotrophs. These results indicate that MSTN was mainly produced in thyrotrophs and its receptor, ActRIIB, was restrictively contained in corticotrophs. Interestingly, thyrotrophs immunoreactive for MSTN were frequently close to corticotrophs immunoreactive for ActRIIB. The present study suggests that MSTN from thyrotrophs may regulate corticotroph function as a paracrine mediator among the porcine anterior pituitary cells. 相似文献
77.
Shuhei Takizawa Ryoki Asano Yasuhiro Fukuda Yasunori Baba Chika Tada Yutaka Nakai 《Animal Science Journal》2021,92(1):e13653
Rumen microorganisms produce various fibrolytic enzymes and degrade lignocellulosic materials into nutrient sources for ruminants; therefore, the characterization of fibrolytic enzymes contributing to the polysaccharide degradation in the rumen microbiota is important for efficient animal production. This study characterized the fibrolytic isozyme activities of a rumen microbiota from four groups of housed cattle (1, breeding Japanese Black; 2, feedlot Japanese Black; 3, lactating Holstein Friesian; 4, dry Holstein Friesian). Rumen fluids in all cattle groups showed similar concentrations of total volatile fatty acids and reducing sugars, whereas acetic acid contents and pH were different among them. Predominant genera were commonly detected in all cattle, although the bacterial compositions were different among cattle groups. Zymograms of whole proteins in rumen fluids showed endoglucanase activities at 55 and 57 kDa and xylanase activity at 44 kDa in all cattle. Meanwhile, several fibrolytic isozyme activities differed among cattle groups and individuals. Treponema, Succinivibrio, Anaeroplasma, Succiniclasticum, Ruminococcus, and Butyrivibrio showed positive correlations with fibrolytic isozyme activities. Further, endoglucanase activity at 68 kDa was positively correlated with pH. This study suggests the characteristics of fibrolytic isozyme activities and their correlations with the rumen microbiota. 相似文献
78.
Shuai SUN Shota YANO Momo O NAKANISHI Michiko HIROSE Kazuhiko NAKABAYASHI Kenichiro HATA Atsuo OGURA Satoshi TANAKA 《The Journal of reproduction and development》2021,67(3):197
Mouse trophoblast stem cells (TSCs) can differentiate into trophoblast cells, which constitute the placenta. Under conventional culture conditions, in a medium supplemented with 20% fetal bovine serum (FBS), fibroblast growth factor 4 (FGF4), and heparin and in the presence of mouse embryonic fibroblast cells (MEFs) as feeder cells, TSCs maintain their undifferentiated, proliferative status. MEFs can be replaced by a 70% MEF-conditioned medium (MEF-CM) or by TGF-ß/activin A. To find out if KnockOutTM Serum Replacement (KSR) can replace FBS for TSC maintenance, we cultured mouse TSCs in KSR-based, FBS-free medium and investigated their proliferation capacity, stemness, and differentiation potential. The results indicated that fibronectin, vitronectin, or laminin coating was necessary for adhesion of TSCs under KSR-based conditions but not for their survival or proliferation. While the presence of FGF4, heparin, and activin A was not sufficient to support the proliferation of TSCs, the addition of a pan-retinoic acid receptor inverse agonist and a ROCK-inhibitor yielded a proliferation rate comparable to that obtained under the conventional FBS-based conditions. TSCs cultured under the KSR-based conditions had a gene expression and DNA methylation profile characteristic of TSCs and exhibited a differentiation potential. Moreover, under KSR-based conditions, we could obtain a suspension culture of TSCs using extracellular matrix (ECM) coating-free dishes. Thus, we have established here, KSR-based culture conditions for the maintenance of TSCs, which should be useful for future studies. 相似文献
79.
Takuya HIROSE Takayuki OGURA Keisuke TANAKA Jun MINAGUCHI Takeshi YAMAUCHI Toshiyuki FUKADA Yoh-ichi KOYAMA Kazushige TAKEHANA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1385-1389
Ehlers-Danlos syndrome (EDS) is a group of disorders caused by abnormalities that are
identified in the extracellular matrix. Transforming growth factor-β1 (TGF-β1) plays a
crucial role in formation of the extracellular matrix. It has been reported that the loss
of function of zinc transporter ZRT/IRT-like protein 13 (ZIP13) causes the spondylocheiro
dysplastic form of EDS (SCD-EDS: OMIM 612350), in which dysregulation of the TGF-β1
signaling pathway is observed, although the relationship between the dermis abnormalities
and peripheral TGF-β1 level has been unclear. We investigated the characteristics of the
dermis of the Zip13-knockout (KO) mouse, an animal model for SCD-EDS.
Both the ratio of dermatan sulfate (DS) in glycosaminoglycan (GAG) components and the
amount of collagen were decreased, and there were very few collagen fibrils with diameters
of more than 150 nm in Zip13-KO mice dermis. We also found that the
TGF-β1 level was significantly higher in Zip13-KO mice serum. These
results suggest that collagen synthesis and collagen fibril fusion might be impaired in
Zip13-KO mice and that the possible decrease of decorin level by
reduction of the DS ratio probably caused an increase of free TGF-β1 in
Zip13-KO mice. In conclusion, skin fragility due to defective
ZIP13 protein may be attributable to impaired extracellular matrix
synthesis accompanied by abnormal peripheral TGF-β homeostasis. 相似文献
80.
Clubroot disease, caused by Plasmodiophora brassicae Woronin, affects various cruciferous crops. Variations in pathogenicity and virulence are present among field populations of P. brassicae. Many races (pathotypes) have been reported in Japan as well as in other countries using various differential systems. Populations can be classified into four pathotypes using two clubroot-resistant (CR) cultivars of Chinese cabbage as differential hosts in Japan. However, it was recently indicated that each population is often heterogenic and composed of multiple genotypes (races or pathotypes). Breakdown in CR cultivars of Chinese cabbage is a problem in some areas of Japan and may contribute to the selective propagation of minor pathogenic genotypes on the CR cultivars. Clubroot has also been recorded on five species of cruciferous weeds in Japan. In particular, clubroot of Cardamine flexuosa is widely distributed in Japan. Some populations of C. flexuosa are often moderately pathogenic on Chinese cabbage and turnip. Therefore, the epidemiological relationship between clubroot of cruciferous crops and that of the weed has been noted but not thoroughly clarified. The relationship between pathogenic and genetic variations has also been examined among populations from cruciferous crops and weeds in Japan. The result implies an interesting genetic relationship among Williams’ races, among pathotypes determined using CR cultivars of Chinese cabbage and among populations from crops and C. flexuosa. This review includes an introduction of the status of studies on pathogenic and genetic diversity in P. brassicae from Japan. 相似文献