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141.
Naoyuki Matsunaga Shin-ichi Chisada Hideyuki Fujioka Kana Takashima Nozomu Okino Makoto Ito 《Fisheries Science》2011,77(4):583-590
Vibrios are highly motile, facultatively anaerobic bacteria that are ubiquitous in aquatic environments and part of the normal
intestinal microflora of healthy fish, but some species can cause vibriosis. The adherence of vibrios to host fish intestines
is a significant event not only for their survival and growth, but also in terms of pathogenicity. However, the molecular
mechanism underlying the adhesion of vibrios to the intestinal tract of fish is not fully understood. We report here the identification
of intestinal glycosphingolipid (GSL) receptors to which pathogenic vibrios attach in typical mariculture fish. Thin-layer
chromatography overlay assays using five species of 35S-labeled vibrios and intestinal glycosphingolipids of seven species of mariculture fish revealed that all of the fish tested
possessed GM3 (NeuAcα2-3Galβ1-4Glcβ1-1′Cer) and/or GM4 (NeuAcα2-3Galβ1-1′Cer) as major acidic intestinal GSLs and that all
of the vibrios tested specifically adhered to GM3 and/or GM4. Our results demonstrate that these GSLs were able to function
as a receptor for the various vibrios tested. Analysis of the relationship between sugar structure and receptor activity for
vibrios revealed that ‘NeuAcα2-3Galβ1-’ is required at the non-reducing end of glycosphingolipids for the bacteria to attach. 相似文献
142.
Dezawa M Ishikawa H Itokazu Y Yoshihara T Hoshino M Takeda S Ide C Nabeshima Y 《Science (New York, N.Y.)》2005,309(5732):314-317
Bone marrow stromal cells (MSCs) have great potential as therapeutic agents. We report a method for inducing skeletal muscle lineage cells from human and rat general adherent MSCs with an efficiency of 89%. Induced cells differentiated into muscle fibers upon transplantation into degenerated muscles of rats and mdx-nude mice. The induced population contained Pax7-positive cells that contributed to subsequent regeneration of muscle upon repetitive damage without additional transplantation of cells. These MSCs represent a more ready supply of myogenic cells than do the rare myogenic stem cells normally found in muscle and bone marrow. 相似文献
143.
144.
Mitsuro KAMEYA-IWAKI Kimiaki MURAKAMI Shin-ichi ITO Kaoru HANADA Shuhei TANAKA 《Journal of General Plant Pathology》2000,66(1):64-67
Sequential transmission tests of Peanut stunt virus (PSV) and Cucumber mosaic virus (CMV) systemically infecting common bean, Phaseolus vulgaris, were conducted using Myzus persicae allowed to fast for 2 hr and then to acquisition feed on infected common bean plants or purified virus for 10 min. In the
sequential transmission tests using either one or 10 aphids per assay plant, three isolates of PSV (J,S,Y5) and one of CMV
(V) were transmitted from and to common bean up to a third or fourth inoculation access. Many aphids transmitted these viruses
to two or three plants. Purified viruses of PSV-S and CMV-V were also transmitted up to a third or second inoculation access
at low percentage. On tobacco, Nicotiana tabacum, aphids transmitted PSV-S and CMV-V only in the first inoculation access, although PSV-S was transmitted to only one plant
in the fourth and fifth inoculation access. These viruses may be transmitted in two phases by aphids, depending on the plant
species.
Received 16 April 1999/ Accepted in revised form 1 September 1999 相似文献
145.
Shin-ichi Masuzaki Tomoya Miyazaki John A. McCallum Sjaak van Heusden Chris Kik Ken-ichiro Yamashita Yosuke Tashiro Naoki Yamauchi Masayoshi Shigyo 《Scientia Horticulturae》2008
Integration of previously developed Allium cepa linkage maps requires the availability of anchor markers for each of the eight chromosomes of shallot (A. cepa L. common group Aggregatum). To this end, eight RAPD markers originating from our previous research were converted into SCAR markers via cloning and sequencing of RAPD amplicons and designing of 24-mer oligonucleotide primers. Of the eight pairs of SCAR primers, seven resulted in the amplification of single bands of the original RAPDs, and the remaining primer set amplified an additional band. The results of Southern hybridization using RAPD amplicons from genomic DNA of Japanese bunching onion (Allium fistulosum L.)—shallot monosomic addition lines indicated that five SCAR markers were single shallot chromosome-specific markers and were not detected in genomic DNA of A. fistulosum. The eight SCAR primer pairs were applied to other Allium species and exhibited three types of amplification profiles, namely RAPD amplicons observed only in shallot, in shallot and Allium vavilovii, and in several Allium species. A mapping study using 65 F2 plants generated by the selfing of one interspecific cross A. cepa × Allium roylei individual integrated the SCAR marker SAOE17500 into chromosome 5 as expected. The results of the present study show that the eight SCAR primer sets specific to shallot can facilitate the mapping in A. cepa and can also serve as anchor points between maps of different Allium species. 相似文献
146.
Toru Kondo Shin-ichi Fuji Kazuo Yamashita Dong-Kyoon Kang Moo-Ung Chang 《Journal of General Plant Pathology》2005,71(6):441-443
We identified Broad bean wilt virus 2 (BBWV-2) in yams based on particle morphology, test plant symptoms, protein features, aphid transmission, and molecular classification
using nucleotide sequences of coat protein genes. 相似文献