全文获取类型
收费全文 | 8071篇 |
免费 | 368篇 |
国内免费 | 999篇 |
专业分类
林业 | 1044篇 |
农学 | 1250篇 |
基础科学 | 618篇 |
1534篇 | |
综合类 | 2192篇 |
农作物 | 505篇 |
水产渔业 | 345篇 |
畜牧兽医 | 1011篇 |
园艺 | 301篇 |
植物保护 | 638篇 |
出版年
2024年 | 16篇 |
2023年 | 89篇 |
2022年 | 220篇 |
2021年 | 343篇 |
2020年 | 272篇 |
2019年 | 278篇 |
2018年 | 210篇 |
2017年 | 267篇 |
2016年 | 262篇 |
2015年 | 353篇 |
2014年 | 345篇 |
2013年 | 405篇 |
2012年 | 461篇 |
2011年 | 520篇 |
2010年 | 542篇 |
2009年 | 501篇 |
2008年 | 443篇 |
2007年 | 391篇 |
2006年 | 451篇 |
2005年 | 432篇 |
2004年 | 200篇 |
2003年 | 168篇 |
2002年 | 136篇 |
2001年 | 148篇 |
2000年 | 181篇 |
1999年 | 270篇 |
1998年 | 222篇 |
1997年 | 205篇 |
1996年 | 177篇 |
1995年 | 136篇 |
1994年 | 132篇 |
1993年 | 136篇 |
1992年 | 121篇 |
1991年 | 111篇 |
1990年 | 80篇 |
1989年 | 67篇 |
1988年 | 47篇 |
1987年 | 25篇 |
1986年 | 27篇 |
1985年 | 11篇 |
1984年 | 10篇 |
1983年 | 5篇 |
1982年 | 7篇 |
1981年 | 7篇 |
1980年 | 1篇 |
1979年 | 7篇 |
排序方式: 共有9438条查询结果,搜索用时 13 毫秒
21.
本文以人肝癌SMMC-7721细胞为材料,对水溶性灵芝提取物(EGL)抗癌作用机理进行了研究。结果表明,EGL能明显抑制SMMC-7721细胞的DNA合成:EGL在50ug/ml到300ug/ml范围表现出明显的剂量效应;EGL最小有效作用剂量为100ug/ml,最大有效作用剂量为300ug/ml。结果提示EGL的抗癌机理主要是通过抑制癌细胞DNA合成而起作用的。 相似文献
22.
23.
不同温度下东方粘虫年龄-阶段两性实验种群生命表的构建 总被引:1,自引:0,他引:1
为明确不同温度对东方粘虫Mythimna separata(Walker)种群动态的影响,建立东方粘虫种群动态模型,利用年龄-阶段两性种群生命表得到了室内不同温度下东方粘虫种群的发育历期、存活率、繁殖力和种群生命表参数,并基于种群生命表参数预测了2个月内东方粘虫的种群动态。结果表明,东方粘虫各虫态的发育历期和产卵前期随温度的升高而显著缩短。24℃下,东方粘虫单雌平均产卵量为717.1粒,显著高于其它温度下;成虫寿命也显著高于其它温度下,且雄蛾寿命显著长于雌蛾,分别为15.3 d和13.0 d;卵孵化存活到成虫的概率、幼虫存活率和化蛹率均最高,分别为66.7%、68.9%和96.8%。内禀增长率随温度的升高而显著增加,平均世代周期则显著降低,18、21、24、27、30℃下分别为0.051、0.077、0.113、0.121、0.156 d-1和77.2、61.3、49.0、40.3、31.5 d;周限增长率也随温度的升高而增加,分别为1.05、1.08、1.12、1.13、1.17 d-1;净增殖率在24℃下为257.1,显著高于其它温度下,其它温度条件下无显著差异。表明东方粘虫在18~30℃范围内都能完成生长发育和生殖,24℃下种群存活率高、繁殖力大、世代周期较短,更适合种群增长。 相似文献
24.
深圳红火蚁蚁巢密度、社会型研究及防治效果评价 总被引:1,自引:0,他引:1
本文报道了深圳红火蚁生境类型与蚁巢密度的关系,社会型的鉴别以及应用茚虫威对多后型和单后型、多后型共存地区的防治效果。红火蚁的蚁巢密度(巢/100 m2)与生境类型密切相关,在所调查的5类生境中,待建地的蚁巢密度最高,为3.81巢/100 m2,公路边的次之,为2.95巢/100 m2,城市绿地的最低,为0.63巢/100 m2。应用多元PCR技术对6个行政区社会型进行鉴别的结果表明,福田、龙岗、南山区为多后型与单后型共存,单后型与多后型的比仅为1∶(3~4)。罗湖、宝安、盐田3区全部为多后型;多后型地区的蚁巢密度(2.2巢/100 m2)比单后型地区(1.9巢/100 m2)的为高,但没有显著差异;应用茚虫威进行防治60 d后的调查结果表明,多后型地区的防治效果(97.8%)比单后型地区(96.7%)的要好,但没有显著差异。最后,本文对这些结果产生的原因及应用价值进行了讨论。 相似文献
25.
26.
保幼激素类似物对红火蚁的作用研究进展 总被引:1,自引:0,他引:1
我国发现红火蚁已有4年,但防治技术、产品依然匮乏。为了改善、提高我国红火蚁的防治技术水平,本文就保幼激素类似物(JHA)对红火蚁的作用表现和防治效果进行了总结分析。JHA可以造成蚁后卵巢萎缩、产卵量减少,导致发育畸形和蚁群等级比例失调,如工蚁数量减少、生殖型幼虫比例以及处女蚁后数量增加等,并最终导致整个蚁群死亡。JHA杀虫剂活性高、低残毒、对环境污染小,在田间防治红火蚁效果彻底,并可有效地防止防治区红火蚁种群的再入侵(re invasion),但JHA毒饵也存在着防治效果缓慢等问题。本文就克服此类问题作了初步的探讨。 相似文献
27.
Direct-fed microbials(DFMs) are feed additives containing live naturally existing microbes that can benefit animals’ health and production performance. Due to the banned or strictly limited prophylactic and growth promoting usage of antibiotics, DFMs have been considered as one of antimicrobial alternatives in livestock industry.Microorganisms used as DFMs for ruminants usually consist of bacteria including lactic acid producing bacteria,lactic acid utilizing bacteria and other bacterial groups,... 相似文献
28.
Qionglin Chen Xinlei Yu Shixia Liu Suya Luo Xiaojiao Chen Nianjun Xu Xue Sun 《Marine drugs》2022,20(7)
Agar is widely applied across the food, pharmaceutical and biotechnology industries, owing to its various bioactive functions. To better understand the agar biosynthesis in commercial seaweed Gracilariopsis lemaneiformis, the activities of four enzymes participating in the agar biosynthesis were detected, and phosphoglucomutase (PGM) was confirmed as highly correlated with agar accumulation. Three genes of PGM (GlPGM1, GlPGM2 and GlPGM3) were identified from the G. lemaneiformis genome. The subcellular localization analysis validated that GlPGM1 was located in the chloroplast and GlPGM3 was not significantly distributed in the organelles. Both the GlPGM1 and GlPGM3 protein levels showed a remarkable consistency with the agar variations, and GlPGM3 may participate in the carbon flux between (iso)floridoside, floridean starch and agar synthesis. After treatment with the PGM inhibitor, the agar and floridean starch contents and the activities of floridean starch synthase were significantly decreased; products identified in the Calvin cycle, the pentose phosphate pathway, the Embden-Meyerhof-Parnas pathway and the tricarboxylic acid cycle were depressed; however, lipids, phenolic acids and the intermediate metabolites, fructose-1,6-phosphate were upregulated. These findings reveal the essential role of PGM in regulating the carbon flux between agar and other carbohydrates in G. lemaneiformis, providing a guide for the artificial regulation of agar accumulation. 相似文献
29.
Linlin Dong Hui Yao Qiushi Li Jingyuan Song Ying Li Hongmei Luo Shilin Chen 《European journal of plant pathology / European Foundation for Plant Pathology》2013,137(4):667-675
Root-knot nematodes (RKNs, Meloidogyne spp.) are damaging pests that can infect thousands of plant species and cause enormous crop losses worldwide. Panax notoginseng is a common host of root-knot nematodes. In this study, we surveyed notoginseng gardens and determined the incidence of RKNs. Among the gardens surveyed, 71 % were infected with RKNs, and the RKN incidence index ranged from 8 % to 47 % in three randomly infected gardens. Meloidogyne hapla was identified as the pathogenic nematode based on 18S ribosomal RNA analysis by DNA barcoding. The results were qualitatively and quantitatively confirmed using a real-time PCR assay according to variations in the ITS1 and ITS2 regions. These results indicated that the combination of DNA barcoding and real-time PCR is a reliable and precise method for identifying parasitic nematodes from mixed-infected plant roots in the field. In addition, the abundance of ITS1 and ITS2 displayed a similar trend to the numbers of RKNs in the three gardens, which suggests that the results of real-time PCR can be used to determine the damage caused by M. hapla in the field. Our studies show that RKNs are common and can cause serious damage to notoginseng. We present an integrated method of detecting mixed nematode species in the field and confirm M. hapla as the target for parasitic nematode control in notoginseng gardens. Our results contribute to the improvement of RKN control in notoginseng and further promote the sustainable development of medicinal plants. 相似文献
30.