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141.
To detect and quantify 1,2-dicarboxylate structures in humic acid, a chemical reaction forming fluoresceins from 1,2-dicarboxylates and resorcinol was tested. Resorcinol reacted with humic acid to form products having absorption maxima at about 490 nm. A procedure was then developed for quantitative analysis of the radicals. Eight humic acid samples tested contained 0.4–1.0 mmol/g of 1,2-dicarboxylate structures. The 1,2-Dicarboxylates detected in humic acid were considered as ophthalate homologs. 相似文献
142.
Oxidative deamination by various polyphenolic compounds is presumed to be due to the oxidative conversion of polyphenols to the corresponding quinones through autoxidation. Here we examined the oxidative deamination by the polyphenol-rich beverages green tea, black tea, and coffee at a physiological pH and temperature. Green tea, black tea, and coffee extracts oxidatively deaminated benzylamine and the lysine residues of bovine serum albumin to benzaldehyde and alpha-aminoadipic delta-semialdehyde residues, respectively, in sodium phosphate buffer (pH 7.4) at 37 degrees C in both the presence and absence of Cu2+, indicating the occurrence of an amine (lysyl) oxidase-like reaction. We also examined the effects of pH and metal ions on the reaction. The possible biological effects of drinking polyphenol-rich beverages on human are also discussed. 相似文献
143.
144.
A single subcutaneous administration of monosodium aspartate (MSA) to 30 neonatal voles, Microtus arvalis Pallas, induced a diabetes mellitus in 50% of the treated animals in early adulthood. The voles (18 males and 12 females) were weaned at 3 weeks of age and fed pellets for Herbivora and cubed hay. Diabetic voles with glycosuria (nine males and six females) were classified into two groups according to the duration and grade of glycosuria. One group had slight diabetes with glycosuria (+: 0.1%) for 1 week and the other severe diabetes with marked glycosuria ( : greater than or equal to 0.5%) for over 4 weeks. Pancreatic islets of diabetic voles (n = 7) were examined immunohistochemically, light microscopically, and electron microscopically. Blood glucose concentration and tissue content of insulin, glucagon, and somatostatin were also measured. Slightly diabetic voles (n = 3) had enlarged islets, that, viewed by light microscopy, were characterized by hypertrophy and hyperplasia of beta cells with moderate degranulation. No changes were observed in the peripherally located alpha and delta cells; the voles were moderately hyperglycemic, and they had decreased pancreatic insulin content. Severely diabetic voles (n = 4) that had marked hyperglycemia and almost complete loss of insulin content showed marked vacuolation and degranulation of beta cells. In addition, altered distribution of alpha and delta cells from the periphery of the islets to their interior was noted. Ultrastructural examination revealed features compatible with those of hyperfunction of beta cells in the slightly diabetic voles and marked degeneration of beta cells with glycogen accumulation in the severely diabetic voles.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
145.
Keaton R. S. Morgan BVSc Ameet Singh DVM DVSc DACVS-SA Michelle A. Giuffrida VMD MSCE DACVS Ingrid M. Balsa DVM DACVS-SA Galina Hayes PhD DACVECC DACVS Margaret L. Chu DVM Christopher B. Thomson DVM Shiori Arai DVM PhD DACVS-SA Daniel D. Smeak DVM MS DACVS Eric Monnet DVM PhD DACVS DECVS Laura E. Selmic BVetMed MPH DACVS-SA DECVS Megan Cray VMD Janet A. Grimes DVM MS DACVS-SA Taylor Morris HBSc J. Brad Case DVM MS DACVS-SA Jeffrey J. Biskup DVM DACVS Jason Haas DVM Kelley Thieman-Mankin DVM MS DACVS-SA Milan Milovancev DVM DACVS-SA Mathieu Gatineau DVM MS DACVS-SA 《Veterinary surgery : VS》2020,49(1):138-145
146.
Philippe Chagnon Larose DVM Ameet Singh DVM DVSc DACVS-SA Michelle A. Giuffrida VMD MSCE DACVS Galina Hayes PhD DACVECC DACVS James Franklin Moyer BSc Janet A. Grimes DVM MS DACVS-SA Jeffrey Runge DVM DACVS Chiara Curcillo DVM Christopher B. Thomson DVM Philipp D. Mayhew BVM&S DACVS-SA Ross Bernstein DVM Christopher Dominic DVM Kelley Thieman Mankin DVM DACVS-SA Penny Regier DVM MS DACVS-SA J. Brad Case DVM MS DACVS-SA Shiori Arai DVM PhD DACVS-SA Mathieu Gatineau DMV IPSAV MSc DACVS DECVS DACVSMR Julius M. Liptak BVSC MVetClinStud FACVSc DACVS-SA DECVS ACVS Charles Bruce DVM DVSc DACVS 《Veterinary surgery : VS》2020,49(5):870-878
147.
Tabara K Kawabata H Arai S Itagaki A Yamauchi T Katayama T Fujita H Takada N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(4):507-510
Endemic spotted fever group rickettsiosis was reported in Shimane Prefecture, Japan. From an analysis of 14 clinical cases found in the endemic area, the infectious agent of spotted fever group rickettsiosis was identified as Rickettsia japonica. In this study, we also found that Rickettsia japonica was highly infected with the vector tick, Haemaphysalis longicornis, in the endemic area. These findings suggest that the high incidence of rickettsiosis in Shimane Prefecture can be explained by the high prevalence of Rickettsia japonica among Haemaphysalis longicornis ticks. 相似文献
148.
Nagano S Kim SH Tokunaga S Arai K Fujiki M Misumi K 《Research in veterinary science》2011,91(3):482-485
We investigated whether matrix metalloproteinase (MMP)-9 expression in the cerebrospinal fluid (CSF) of dogs with intervertebral disc herniation (IVDH) is associated with the severity of neurological signs and prognosis. CSF from the cisterna magna (C-CSF) and the lumbar spine (L-CSF) of 34 dogs with IVDH was analyzed using zymography. Activity of MMP-9 in L-CSF was detected in 6 of 34 dogs with IVDH, often for more than 7 days after injury. MMP-9 activity was not detected from any of the C-CSF samples. Of the six cases that were MMP-9 positive, all four cases with grade V that had loss of deep pain were non-ambulatory 6 months after treatment. The remaining two cases with grade III and IV could recover mobility. In dogs with grade V thoracolumbar IVDH, MMP-9 expression in the CSF may indicate severe spinal cord injury with poor prognosis. 相似文献
149.
Takaomi Arai 《Fisheries Science》2006,72(2):382-387
ABSTRACT: The habitat use and migration of ayu Plecoglossus altivelis was compared to that of ice goby Leucopsarion petersi using otolith microchemistry analysis. Both species were collected along the Sanriku Coast. Otolith Sr : Ca ratios of ayu fluctuated strongly along the life history transect in accordance with the migration (habitat) pattern from fresh water to sea water. The Sr : Ca ratios of the center region averaged 3.2 × 10−3 ; thereafter, the ratios increased sharply, averaging 9.2 × 10−3 , and were maintained at the higher levels until the outermost regions. By contrast, the Sr : Ca ratios of ice goby showed consistently high values along the life history transect of the otolith, ranging 9.0 × 10−3 in the center to 9.2 × 10−3 in regions outside the center, with further increases around the otolith edge. These findings indicate that ayu shows a typical amphidromous migratory pattern, while ice goby does not show the anadromous migratory pattern previously reported. The use of a freshwater environment during the early developmental stage in ice goby along the Sanriku Coast was less prominent than that of ayu in the same region. 相似文献
150.
Takashi Yokota Reiji Masuda Hiroyuki Takeuchi Tatsuo Tsuzaki Nobuaki Arai 《Fisheries Science》2007,73(3):500-511
ABSTRACT: To determine characteristics which may reflect the behavioral quality of red tilefish Branchiostegus japonicus after release, five wild and four hatchery-reared fish were observed in a laboratory and in the sea. The diel activity of the fish was recorded on video tape for 2 days in a tank, where the light condition was regulated to vary periodically over 24 h. The individuals were then released in Maizuru Bay and tracked using acoustic telemetry. Four wild individuals became most active at dawn during a day in the tank, and settled near the release area to stay for 28–104 days after release in Maizuru Bay. In contrast, one wild and four hatchery-reared individuals were not active at dawn, and then did not settle in Maizuru Bay after release. Among the four settled wild individuals, the more active a fish had been in the tank, the more distant the individual moved from the release point until its settlement. The diel activity pattern should be a factor deciding whether red tilefish would settle nearby after release, and the relative amount of activity during rearing is probably related to the degree of dispersion within the release area. 相似文献