Physiological Effect of New FA Antitranspirant Application on Maize

The field trial was conducted to study physiological effect of new FA antitranspirant on maize.The new FA antitranspirant was sprayed at 10 d pre-tasseling, ear filling stage and 10 d pre-tasseling + earfilling stage, with the concentrations of 0, 0.5, 1.0, 1.5 and 2.0 ml L-1. The results indicated that the appli-cation of new FA antitranspirant increased nitrate reductase activity (NRA), free proline content, chlorophyllcontent and water content of leaf, thus drought stress can be mitigated. The new FA antitranspirant increasedphotosynthesis rate and reduced transpiration rate, stimulated growth and reduced water loss. 10 d pre-tassel-ing + ear filling stage application had a cumulative effect on the indices compared with 10 d pre-tasseling andear filling stage, except for NRA. The new FA antitranspirant caused an increase of grain yield by 5.37 to29.58 % with different treatments. The optimal concentration is 1.5 ml L-1 , i.e. 75 g new FA antitranspirantdissolved in 50 kg water, and the optimal apply period is 10 d pre-tasseling ± ear filling stage.     

应用竞争PCR检测丙酸盐对体外培养牛肝细胞丙酮酸羧化酶mRNA水平的影响

根据牛肝细胞内PC(丙酮酸羧化酶)基因组与PCcDNA相比多含有一个内含子序列．在其中再插入一外源DNA序列．从而使最终构建的突变体片段的长度大于PCcDNA的长度，成功构建了PCcDNA的竞争DNA模板，然后应用竞争PCR方法研究了丙酸盐对体外培养新生牛单层肝细胞PCmRNA水平的影响。使单层肝细胞培养液中丙酸钠浓度分别为0、1．5、2．5、3．5、4．5、8．5、11．5mmol／L，处理24h，提取总RNA、逆转录，在同一体系中用相同引物扩增目的带和竞争模板带。结果表明．随着丙酸钠浓度的升高．PCmRNA水平呈上升趋势，提示肝细胞内PCmRNA的表达水平受培养液中丙酸钠浓度的影响。     

室内空气中甲醛污染的危害与控制方法浅析

本文者重阐述室内空气中的主要污染物甲醛对人身健康的危害．同时分析甲醛污染的各种可能性来源．并且简要介绍了现今比较常见的几种控制甲醛污染的切实可行措施和手段。     

间接酶联免疫吸附试验(ELISA)检测血清4型鸭疫里默氏杆菌抗体的研究

应用超声波裂解珐制备的血清4型鸭疫里默氏杆菌(RA)裂解物作为抗原包被酶标板，成功建立了4型RA抗体检测的间接ELISA法。     

Nature's Concept. The ‘New Agriculture’ amidst Ecology,Economy and the Demythologization of the Gene

From worldwide discussions about the limits of agriculture has arisen a powerful vision of sustainable development. Phrases like ‘limitation’, ‘sustainability’, ‘sufficient supply’, ‘fair distribution’ and ‘productivity of resources’ are no longer hindrances but guidelines for a fairer and safer world. ‘Nature's Concept' focuses on biological and ecological principles. On this basis, criteria of action have to be defined in order to determine long‐term economic consequences of agri‐production. The costs of destruction of natural resources, such as reduction of genetic resources, changes to the world climate and soil degradation, are inestimably large. The long‐term effects show the real substance of the agri‐shift and the demands on research. The solution for sustainable agriculture is more than ever a scientific development of ‘tools’ for a sensible management of resources. Gene technology may be an option to reduce the ecological risks of agriculture, especially those of devastating land use. For this purpose, new education programmes with international links and interventions by states are crucial in order to solve conflicts when short‐term interests interfere with long‐term ecological values. The international agri‐centres could have a further impact to guarantee access to new techniques for all countries. Thus agriculture could finally become a major pillar in a fair world order.     

Anti‐microbial Susceptibility for east1+Escherichia coli Isolated from Diarrheic Pigs in Korea

The in vitro susceptibilities of 128 isolates of east1⁺Escherichia coli from pre‐weaned and post‐weaned pigs with diarrhoea were tested with nine commonly used anti‐microbial agents by an agar dilution minimal inhibitory concentration (MIC) procedure according to National Committee for Clinical Laboratory Standards guidelines. For the isolates from pre‐weaned and post‐weaned pigs, most of them were susceptible to low concentrations (MIC₉₀) of tetracycline (4 and 2 μg/ml), ceftiofur (2 and 2 μg/ml), and colistin (4 and 2 μg/ml). Marked resistance was found in others.     

应用电镜技术快速检测培养细胞中的支原体污染

应用电镜技术可快速准确地检测培养细胞中的支原体污染。通过高速离心浓缩样品,负染色后电镜观察,可在30min内做出判定,方法快速、简便、准确。     

sch蚕胚胎期温敏性的mRNA差异显示研究

采用mRNA差异显示技术对不同催青处理的伴性赤蚁sch系统进行研究 ,并以普通黑蚁系统夏芳作为对照。实验中分别抽提高温和常温催青处理 36h后的sch蚕卵和夏芳蚕卵的总RNA进行DDRT -PCR分析 ,其产物于变性聚丙烯酰胺凝胶上电泳展示 ,得到大量的差示片段。对高温催青处理后在夏sch蚕卵中特异表达的 2条能稳定重复的带进行了克隆 ,得到了长度分别为 2 73和 199bp的cDNA片段 ,并对其进行了序列分析。Northern杂交证实HAP82 80仅在高温催青处理的夏sch蚕卵中有转录 ,表明其为sch蚕卵经高温催青处理后特异表达的产物     

大坝防渗层新浇筑砼的水化热温度应力分析

在对大坝坝段进行温度场及温度应力的有限元分析基础上，采用后差分法推导了求解瞬态温度场有限元基本方程，编制了计算程序进行计算。着重对防渗层浇筑过程中因混凝土水化热产生的温度应力进行了研究，其中考虑了自生体积膨胀、应力徐变的影响，得出了对工程指导有益的结论。