Characterization of Zinc Oxide Nano Particles and Their Effect on Growth of Maize (Zea mays L.) Plant

In the current literature, the impact of nano-particles (NPs) on growth of higher plants has scantly been reported. An investigation was carried out to study the effect of zinc oxide nano-particles (<100 nm) on growth of maize (Zea mays L.) plant, as one of the major agricultural crops, in a solution culture system. Various concentrations of zinc (Zn) were applied through nano-zinc oxide (ZnO) particles (<100 nm) in suspension form and in ionic form through zinc sulfate (ZnSO₄) salt in Hoagland solution culture. Experimental results showed that nano zinc oxide particles could enhance and maintain the growth of maize plant as well as conventional Zn fertilizer (as ZnSO₄). The plant parameters like plant height, root length, root volume, and dry matter weight were all improved due to application of zinc oxide nano-particle. These findings indicate that plant roots might have the unique mechanism of assimilating nano-Zn and using for its growth and development. Different enzymatic activities were also studied and experimental results revealed that nano-ZnO particles (<100 nm) also governed the enzymatic activity of maize plant. A separate laboratory experiment was also carried out to characterize the zinc oxide nano particle for its size, zeta potential, etc.     

Enhanced soybean (Glycine max L.) plant growth and nodulation by Bradyrhizobium japonicum-SB1 in presence of Bacillus thuringiensis-KR1

Abstract

Nodulation and subsequent nitrogen fixation are important factors that determine the productivity of soybean (Glycine max L.). The beneficial effects of nodulation can be enhanced when rhizobial inoculation is combined with plant-growth-promoting bacteria (PGPB). The PGPB strain Bacillus thuringiensis-KR1, originally isolated from the nodules of Kudzu vine (Pueraria thunbergiana), was found to promote growth of soybean plants (variety VL Soya 2) under Jensen's tube and growth pouch conditions, when co-inoculated with Bradyrhizobium japonicum-SB1. Co-inoculation with Bacillus thuringiensis-KR1 (at a cell density of 10 cfu) provided the highest and most consistent increase in nodule number, shoot weight, root weight, root volume, and total biomass, over rhizobial inoculation and control, under both conditions. The results demonstrate the potential benefits of using nonrhizobial nodule occupants of wild legumes for the co-inoculation of soybean, with Bradyrhizobium japonicum-SB1, in order to achieve plant-growth promotion and increased nodulation.     

Differential response of phytohormone signalling network determines nonhost resistance in rice during wheat stem rust (Puccinia graminis f. sp. tritici) colonization

Stem rust caused by Puccinia graminis f. sp. tritici is one of the most devastating diseases of wheat. Breakdown of host resistance under field conditions triggered by the evolution of new pathogenic races and pathotypes is a perennial threat for wheat cultivation. Rice, often grown in a rice–wheat cropping system, is immune to rust infection. Our microscopic studies revealed that P. graminis f. sp. tritici, although displaying nearly identical uredospore germination, stomatal entry, and epi- and endophytic mycelial growth in rice and wheat, failed to sporulate to cause rust disease in rice. We identified 18 key defence signalling genes in rice and unravelled their elicitation dynamics in time-course studies during infection. ICS1, NPR1-3, PRs, EDS1, PAD4, FMO1 (salicylic acid [SA] signalling), and ethylene-related genes (ACO4 and ACS6) were strongly elicited in rice. However, genes from the jasmonic acid (JA) signalling pathway (LOX2, AOS2, MYC2, PDF2.2, JAZ8, JAZ10) showed a delayed response during colonization in rice compared to an early or no induction in wheat. However, the JA/ethylene marker gene PDF2.2 was strongly induced in wheat as early as 12 hr postinoculation. Furthermore, rice and wheat displayed specific profiles of accumulation of various phenolic acids during P. graminis f. sp. tritici 40A infection. We propose a model where a differential modulation of the SA/JA-dependent defence network may modulate nonhost resistance. A deeper understanding of the molecular mechanism governing differential elicitation of defence signalling may provide a novel resistance mechanism for the sustainable management of rust diseases.     

Nutritional evaluation of wheat straw treated with Crinipellis sp. in Sahiwal calves

Wheat straw was subjected to solid-state fermentation (SSF) with lignolytic white-rot fungus (WRF) Crinipellis sp. for 5 days to improve the nutritive value and digestibility. The fungal treatment caused a significant (P?<?0.05) decrease in cell wall constituents viz., neutral detergent fiber (NDF), acid detergent fiber (ADF), hemicellulose, lignin, and cellulose to the extent of 10.4, 11.2, 8.7, 8.7, and 12.1 %, respectively, with increase (P?<?0.05) in crude protein (CP) (51.6%) and ash (25.8%) contents in fungal treated wheat straw (FT-WS) than untreated wheat straw (UT-WS). Further, in vitro gas production, in vitro true dry matter digestibility and in vitro true organic matter digestibility at 48 h, metabolizable energy (ME) content, microbial biomass production, and short-chain fatty acids synthesis were significantly (P?<?0.05) higher in FT-WS. In vivo feeding trial in 10 Sahiwal calves (8–12 months) comprised of (1) control group (T1) fed with ad libitum chopped UT-WS and (2) treatment group (T2) offered with ad libitum chopped FT-WS, in addition to supplementation of groundnut cake and green berseem (Trifolium alexandrium) forage to both groups. Digestibility of nutrients for dry matter (DM), organic matter, CP, NDF, ADF, hemicellulose, cellulose, and total carbohydrates were significantly (P?<?0.05) higher in T2 compared to T1. Moreover, daily DM (P?<?0.05), digestible crude protein (P?<?0.01), and ME intakes were also higher (P?<?0.05) in group T2 with higher (P?<?0.05) nitrogen (N) retention, which resulted in significantly (P?<?0.05) higher average daily gain in body weight (135 vs. 102 g/day). It was concluded that SSF with WRF Crinipellis sp. holds potential in upgrading the nutritional worth of wheat straw for feeding growing calves.     

Prevalence and risk factors associated with Cryptosporidium spp. infection in young domestic livestock in India

A total of 938 faecal samples (461 cattle calves, 264 buffalo calves, 55 lambs, 116 kids and 42 piglets) from different livestock farms and individual small holdings in six targeted states of India were collected and screened by modified Ziehl–Neelsen staining technique to determine the prevalence of Cryptosporidium spp. and its association with age, sex, season and faecal consistency in domesticated animals. Overall, 16.2 % of the animals were positive for Cryptosporidium infection with prevalence of 16.3, 24.2, 1.8, 3.5 and 19.1 % in cattle calves, buffalo calves, lambs, kids and piglets, respectively. The prevalence of infection was significantly higher (p?<?0.05) in bovines (19.3 % cattle and 33.7 % buffalo) below 1 month of age than in animals between 1 and 3 months of age. But in piglets, it was higher in the age group of 1 to 3 months (22.6 %) than in younger animals (9.1 %). Also, higher prevalence (p?>?0.05) was recorded in females than in males. Seasons had a significant effect (p?<?0.05) on the prevalence of infection in large ruminants, with the highest prevalence in monsoon (cattle 28.8 % and buffalo 36.6 %) followed by pre-monsoon and post-monsoon season. However, in case of sheep and goats, the prevalence was higher (p?>?0.05) in post-monsoon than in monsoon season. A high degree of association was noticed between Cryptosporidium infection and diarrhoea in ruminants screened during the present study. But, in case of pigs, the prevalence was higher in non-diarrhoeic than in diarrhoeic animals. Genotyping of Cryptosporidium spp. based on nested PCR amplification of partial 18S rRNA and its subsequent digestion with SspI, VspI and MboII restriction enzymes revealed prevalence of Cryptosporidium parvum in representative number of positive samples of cattle, buffalo and goats.     

Different indices to characterize water use pattern of irrigated cauliflower (Brassica oleracea L. var. botrytis) in a hot sub-humid climate of India

Frequency and depth of irrigation play crucial role in crop yield and use efficiency of water resource. To test this hypothesis a field study was carried out in November to January of 2001-2002 to 2003-2004 on a sandy loam (Aeric haplaquept) for quantifying the frequency and depth of irrigation on growth, curd yield (CY) and water use pattern of cauliflower (Brassica oleracea L. var. botrytis). Four irrigation frequencies depending on the attainment of cumulative pan evaporation (CPE) values of: 25 (CPE₂₅), 31(CPE₃₁), 38 (CPE₃₈) and 45 (CPE₄₅) mm were placed in main-plots, with three depth of irrigation (IW) of 35 (IW₃₅), 30 (IW₃₀) and 25 (IW₂₅) mm in sub-plots. Water use efficiency (WUE), net evapotranspiration efficiency (WUE_ET) and irrigation water use efficiency (WUE_I) were computed. Marginal water use efficiency (MWUE) and elasticity of water productivity (EWP) were calculated using the relationship between CY and seasonal actual evapotranspiration (SET). A continuous increasing trend in growth parameters, yield and WUE_I was recorded with the increase in SET from CPE₄₅-IW₂₅ to CPE₃₁-IW₃₀. However with further increase in SET the same decreased up to CPE₂₅-IW₃₅ regime. Highest WUE and WUE_ET obtained under CPE₃₈-IW₃₅ regime where SET value was 5% lower than the status of SET under CPE₃₁-IW₃₀. This study confirmed that critical levels of SET needed to obtain maximum curd yield or WUE, could be obtained more precisely from the knowledge of MWUE and EWP.     

Soil Carbon Sequestration in Long-Term Fertilization Under Jute-Rice-Wheat Agro-Ecosystem

Soil organic carbon (SOC) sequestration in response to long-term fertilizer management practices under jute-rice-wheat agro-ecosystem in alluvial soils was studied using a modeling approach. Fertilizer management practices included nitrogen (N), phosphorus (P) and potassium (K) fertilization, manure application, and root-stubble retention of all three crops. Soil carbon (C) model RothC was used to simulate the critical C input rates needed to maintain initial soil C level in long timescale (44 years). SOC change was significantly influenced by the long-term fertilizer management practices and the edaphic variable of initial SOC content. The effects of fertilizer combination “100%NPK+FYM” on SOC changes were most significant over “100%NPK” fertilization. If the 100% NPK fertilizer along with manure applied with stubble and roots retention of all crops, alluvial soils of such agro-ecosystem would act as a net C sink, and the average SOC density kept increasing from 18.18 Mg ha^?1 during 1972 to the current average of ～22 Mg ha^?1 during 2065 s. On an average, the critical C input was estimated to be 5.30 Mg C ha^?1 yr^?1, depending on local soil and climatic conditions. The critical C input could be effectively estimated using a summary model driven by current SOC level, mean annual temperature, precipitation, and soil clay content. Such information will provide a baseline for assessing soil C dynamics under potential changes in fertilizer and crop residues management practices, and thus enable development of management strategies for effectively mitigating climate change through soil C sequestration.     

Long-term yield trend and sustainability of rainfed soybean–wheat system through farmyard manure application in a sandy loam soil of the Indian Himalayas

A long-term (30 years) soybean–wheat experiment was conducted at Hawalbagh, Almora, India to study the effects of organic and inorganic sources of nutrients on grain yield trends of rainfed soybean (Glycine max)–wheat (Triticum aestivum) system and nutrient status (soil C, N, P and K) in a sandy loam soil (Typic Haplaquept). The unfertilized plot supported 0.56 Mg ha⁻¹ of soybean yield and 0.71 Mg ha⁻¹ of wheat yield (average yield of 30 years). Soybean responded to inorganic NPK application and the yield increased significantly to 0.87 Mg ha⁻¹ with NPK. Maximum yields of soybean (2.84 Mg ha⁻¹) and residual wheat (1.88 Mg ha⁻¹) were obtained in the plots under NPK + farmyard manure (FYM) treatment, which were significantly higher than yields observed under other treatments. Soybean yields in the plots under the unfertilized and the inorganic fertilizer treatments decreased with time, whereas yields increased significantly in the plots under N + FYM and NPK + FYM treatments. At the end of 30 years, total soil organic C (SOC) and total N concentrations increased in all the treatments. Soils under NPK + FYM-treated plots contained higher SOC and total N by 89 and 58% in the 0–45 cm soil layer, respectively, over that of the initial status. Hence, the decline in yields might be due to decline in available P and K status of soil. Combined use of NPK and FYM increased SOC, oxidizable SOC, total N, total P, Olsen P, and ammonium acetate exchangeable K by 37.8, 42.0, 20.8, 30.2, 25.0, and 52.7%, respectively, at 0–45 cm soil layer compared to application of NPK through inorganic fertilizers. However, the soil profiles under all the treatments had a net loss of nonexchangeable K, ranging from 172 kg ha⁻¹ under treatment NK to a maximum of 960 kg ha⁻¹ under NPK + FYM after 30 years of cropping. Depletion of available P and K might have contributed to the soybean yield decline in treatments where manure was not applied. The study also showed that although the combined NPK and FYM application sustained long-term productivity of the soybean–wheat system, increased K input is required to maintain soil nonexchangeable K level.     

Comparative analysis of seed morphometric and allozyme data among four populations of neem (Azadirachta indica)

The objectives of this study were to determine the patterns of variability and to compare genetic distance between seed morphometric and allozyme data of neem (Azadirachta indica). Phenotypic variation from open pollinated seed materials at 12 isozyme loci was examined using starch gel electrophoresis for four populations from Thailand, Bangladesh and Kenya. All four populations exhibited high levels of variation for seed parameters and isozymes. UPGMA cluster analysis of Nei's genetic distance when based on isozymes revealed two genetic groups. The population from Kenya showed a close genetic relationship with the Bangladeshi populations. The population from Thailand exhibited a distant relationship with the other three populations. This result suggests that the Kenyan population was introduced from the Indian subcontinent. In contrast, cluster analysis of seed morphometric data indicated three different groups of populations. The population from Kenya showed a distant relationship with all other populations. The study demonstrates that allozyme data may not correspond with morphometric traits when measuring genetic distance. It is suggested that this controversy may depend on environmental influence on morphometric traits, possibly also on the enzyme patterns.