Direct monitoring of Rhizobium sp. (Cicer) in nodules and soil using lac Z fusions

We constructed lacZ fusions in Rhizobium sp. (Cicer) by random Tn5-lacZ mutagenesis. The lacZ⁺ fusants formed blue colonies on a Rhizobial minimal medium containing 5-bromo-4-chloro-3-indolyl--D-galactopyranoside (X-gal). Rhizobium sp. (Cicer) fusant HSL-2 was identified in nodules and soil in a mixed population on the basis of the lacZ⁺ phenotype. Nodule occupancy of inoculated Rhizobium sp. (Cicer) HSL-2 (lacZ⁺) was assessed by directly streaking the nodule sap on X-gal plates. This method revealed differences between rhizobia carrying identical antibiotic markers. The rhizobial population in soil was estimated by direct plate counts using a medium containing X-gal. Introduction of lacZ into the Rhizobium sp. thus provided a simple and direct method for identifying strains from nodules and soil.     

Growth and reproduction of the vermicomposting earthwormPerionyx excavatus as influenced by food materials

An outdoor study was undertaken using polyethylene containers to assess the suitability of different organic residues, soybean straw (Glycine max L. Merril.), wheat straw (Triticum aestivum L.), maize stover (Zea mays L.), chickpea straw (citer arietinum L.) and city garbage, as food for the tropical epigeic earthwormPerionyx excavatus, and to assess the influence of this earthworm on the decomposition of these materials. Maize stover was found to be the most suitable of the food materials used. Population growth ofP. excavatus was enhanced by addition of these organic materials in the temperature range 24°-30°C, while the population was adversely affected above 30°C in a vermiculture system. Addition of earthworms accelerated the breakdown of residues, which ultimately resulted in a lowering of the C:N ratio, water-soluble carbon and carbohydrates, and increased ash percentage and cation exchange capacity compared with their respective controls.     

Net oxidative addition of C(sp3)-F bonds to iridium via initial C-H bond activation

Carbon-fluorine bonds are the strongest known single bonds to carbon and as a consequence can prove very hard to cleave. Alhough vinyl and aryl C-F bonds can undergo oxidative addition to transition metal complexes, this reaction has appeared inoperable with aliphatic substrates. We report the addition of C(sp(3))-F bonds (including alkyl-F) to an iridium center via the initial, reversible cleavage of a C-H bond. These results suggest a distinct strategy for the development of catalysts and promoters to make and break C-F bonds, which are of strong interest in the context of both pharmaceutical and environmental chemistry.     

Characterization of Tolerance Limit in Spirulina platensis in Relation to Nanoparticles

A study was carried out under in vitro conditions to characterize the growth of blue green alga, Spirulina platensis, in standard CFTRI medium containing different nanoparticles of copper oxide (CuO) (50 nm, 10 ppm), zinc oxide (ZnO) (50 nm, 10 ppm), tricalcium phosphate (TCP) (<100 nm, 90 ppm), and hydroxy apatite (HA) (<200 nm, 90 ppm). S. platensis exhibited significant higher growth in standard CFTRI medium containing 90 ppm phosphorus as nanoparticles of TCP and HA. On the other hand, calcium phosphate nanoparticles caused significant reduction in nitrate reductase activity as well as in protein content of the alga. Marked change in chlorophyll-a/b ratio was also noted when phosphorus was supplied through nano tricalcium phosphate and nano hydroxy apatite particles as compared to ionic form (K₂HPO₄). The study revealed that the growth of Spirulina in the presence of ZnO nanoparticles was retarded, while no growth was observed with CuO nanoparticles. It was concluded that alga Spirulina showed much sensitivity to nanoparticles of zinc and copper (<50 nm) and was able to tolerate the toxicity of nanophosphate (tricalcium phosphate <100 nm; hydroxy apatite <200 nm).     

In-vitro characterization of the behaviour of Macrophomina phaseolina (Tassi) Goid at the rhizosphere and during early infection of roots of resistant and susceptible varieties of sesame

There are major gaps in our knowledge of the stages of infection in soil borne pathogens. Soil borne diseases, such as charcoal root rot caused by Macrophomina phaseolina on sesame, have been studied, yet due to the difficulty in observing fungal behaviour in the soil, there has been no detailed study of the infection events. Moreover no study has attempted to compare the infection events in roots of resistant versus susceptible hosts. We present the first ultra-structural report to characterize the behaviour of the fungus in the proximity of the root, the appearance of fungal hyphae on the surface of roots, microsclerotia formation on hyphal strands, early penetration events and subsequent infection processes of M. phaseolina in sesame. We observed distinct differences in fungal behaviour in the rhizosphere and during infection of susceptible and resistant varieties. This study also describes a framework for comparative experiments. The possible reasons for the difference in behaviour of M. phaseolina in the vicinity of and during infection of roots of resistant vs. susceptible varieties of sesame and its implications for disease resistance are discussed.     

Comparison between fertilizers and untreated sewage effluent as sources for nutrient recovery by wheat (Triticum aestivum)-soybean (Glycine max) cropping system

Recovery and recycling of plant nutrients from municipal sewage effluent (SE) can reduce consumption of costly chemical fertilizers, besides reducing eutrophication of water bodies. Field experiment was conducted on Vertisol of central India for six years with the objective of quantifying recovery of major plant nutrients by aboveground biomass of wheat-soybean cropping system from untreated SE. Wheat crop was grown with irrigation (groundwater and sewage effluent) and fertilizer treatments; while soybean was grown without any treatments. Recoveries of nitrogen (N), phosphorus P) and potassium (K) by the aboveground biomass were considerably more from SE than from fertilizers and manures. Recovery of nutrients from SE was the highest by wheat grain for N and by soybean straw for P and K. Straw biomass of both the crops recovered about 31% N, 22% P and 69% K from SE, which can be recycled back into agricultural land of groundwater (GW) irrigated as well as rainfed area.     

Optimizing Seed Sample Size for Zinc and Iron Analysis of Wild and Cultivated Lentil

Zinc and iron deficiencies affect billions of people globally and have direct impact on human health. Zinc and iron concentrations in plant materials are commonly analyzed by flame atomic absorption spectrometry (F-AAS). This research describes the optimal analysis method for precise zinc (Zn) and iron (Fe) analysis of seeds from the seven species of genus Lens. Estimation of Zn and Fe concentration in whole lentil seeds by F-AAS is destructive, using nitric acid digestion of seeds prior to instrument analysis. The procedure proposed for the determination of Zn and Fe concentration uses the minimum amount of lentil seed of all lentil species with suitable standard reference materials. The relative standard deviations of the method were about 5% for both Zn and Fe. The minimum sample amount 0.3 g of wild and 0.5 g of cultivated lentil seed samples was identified for accurate and precise estimation of Zn and Fe concentration.     

Carbon and nutrient dynamics under long-term nutrient management in tropical rice-wheat-jute system

Impacts of 43-year nutrient management on carbon (C) and nutrient dynamics were studied in a rice-wheat-jute system in tropical India. Carbon dynamics was investigated on the basis of its distribution of labile (microbial biomass C, permanganate oxidizable C and water soluble C) and slow pools (Walkley Black oxidizable organic C), its indices (C pool index, lability index) and C management index (CMI) at different soil depths. Nutrient dynamics was judged by major nutrients availability, and related soil enzymatic activities. We studied seven treatments including unfertilized control, 100% NPK (N:P₂O₅:K₂O 60:30:60), 50% NPK, 150% NPK, 100% NP, 100% N, 100% NPK + farm yard manure (10 tonnes FYM ha^?1). Nutrients availability and soil enzymatic activities were significantly increased under balanced fertilization (100% NPK + FYM) over other treatments. Labile, slow and total C was also found highest in 100% NPK + FYM. Enhanced C indices under 100% NPK + FYM over 100% NPK and other treatments signified the importance of long term balanced fertilization on soil C stabilization. Lability indexes were lower at sub-soil (30-45cm). In terms of favourable nutrient dynamics and CMI, balanced application of NPK plus organics were recommend for long term sustainability of rice-wheat-jute system in tropics.     

Sulfur accumulation in Vertisols due to continuous gypsum application for six years and its effect on yield and biochemical constituents of soybean (Glycine Max L. Merrill)

Application of S through inorganic sources is recommended to the crops in the cropping system in S deficient soils of India. However, transformations of S in the soil profile following continuous gypsum application are not known. The accumulation of S into different fractions due to application of a total of 180—540 kg S ha^—1 over a six‐year‐period in soybean — wheat cropping system and its effect on the yield and quality of soybean produce were investigated in a vertisol area. About 25—38% of the S added was retained in the 90 cm depth of profile. Significant changes in the total S content in soil layers were recorded wherein a total of 360 kg S ha^—1 or more S was applied. Maximum increase in total S content was recorded in upper layers. Soluble S accumulated more in the 30—75 cm depth; whereas, proportion of sorbed fraction of S increased with depth. Build up of S in the surface layer of the profile was mostly related to the increase in organic fraction. The S accumulation in lower layers of the profile was mostly due to increase in soluble and sorbed fractions of S. Such an increase of the S content in the soil caused an increase in seed yield, uptake of S as well as content of oil and S containing amino acids, cystine, and methionine in seed of soybean crop.