全文获取类型
收费全文 | 17153篇 |
免费 | 40篇 |
国内免费 | 1篇 |
专业分类
林业 | 3684篇 |
农学 | 1317篇 |
基础科学 | 137篇 |
2855篇 | |
综合类 | 729篇 |
农作物 | 2132篇 |
水产渔业 | 1881篇 |
畜牧兽医 | 1433篇 |
园艺 | 1117篇 |
植物保护 | 1909篇 |
出版年
2024年 | 1篇 |
2023年 | 2篇 |
2022年 | 21篇 |
2021年 | 42篇 |
2020年 | 25篇 |
2019年 | 12篇 |
2018年 | 2755篇 |
2017年 | 2716篇 |
2016年 | 1215篇 |
2015年 | 95篇 |
2014年 | 66篇 |
2013年 | 74篇 |
2012年 | 833篇 |
2011年 | 2170篇 |
2010年 | 2120篇 |
2009年 | 1275篇 |
2008年 | 1360篇 |
2007年 | 1624篇 |
2006年 | 93篇 |
2005年 | 149篇 |
2004年 | 144篇 |
2003年 | 173篇 |
2002年 | 93篇 |
2001年 | 22篇 |
2000年 | 49篇 |
1999年 | 3篇 |
1998年 | 8篇 |
1995年 | 4篇 |
1993年 | 12篇 |
1992年 | 7篇 |
1990年 | 1篇 |
1989年 | 5篇 |
1988年 | 11篇 |
1987年 | 1篇 |
1985年 | 1篇 |
1977年 | 4篇 |
1975年 | 1篇 |
1972年 | 1篇 |
1969年 | 1篇 |
1968年 | 4篇 |
1967年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
71.
Concepta McManus Helder Louvandini Rosilene Gugel Luiz Cláudio Bastos Sasaki Eliandra Bianchini Francisco Ernesto Moreno Bernal Samuel Rezende Paiva Tiago Prado Paim 《Tropical animal health and production》2011,43(1):121-126
The objective of this study was to evaluate and compare physical parameters in groups of sheep with different phenotypic characteristics
in the Centre-west region of Brazil. Five groups of sheep, with nine animals per group, were selected, three groups of Santa
Inês animals with different coat colours (white, brown and black), one group with crossbred animals (Santa Inês × Bergamasca)
and one group with animals of the Bergamasca breed. The following traits were evaluated: coat thickness, number and length
of hair, pigmentation level in the coat and the skin as well as the percentage area of sweats glands in the skin tissue, carried
out by histological analysis. The number of hairs and the area of sweats glands were not significantly different between the
evaluated groups. The Bergamasca breed showed low pigmentation of the skin and long hairs. The levels of pigmentation of the
hair and of the skin were highly correlated. Between the Santa Inês groups, the group with white hair showed the better parameters
for heat adaptation, while the brown hair group showed the lower heat adaptation when compared with another hair breed groups. 相似文献
72.
Mi Jeong Park Jienny Lee Jeong Su Byeon Da-Un Jeong Na-Yeon Gu In-Soo Cho Sang-Ho Cha 《Veterinary research communications》2018,42(3):171-181
Mesenchymal stem cells (MSCs) are useful candidates for tissue engineering and cell therapy fields. We optimize culture conditions of equine adipose tissue-derived MSCs (eAD-MSCs) for treatment of horse fractures. To investigate enhancing properties of three-dimensional (3D) culture system in eAD-MSCs, we performed various sized spheroid formation and determined changes in gene expression levels to obtain different sized spheroid for cell therapy. eAD-MSCs were successfully isolated from horse tailhead. Using hanging drop method, spheroid formation was generated for three days. Quantitative real-time PCR was performed to analyze gene expression. As results, expression levels of pluripotent markers were increased depending on spheroid size and the production of PGE2 was increased in spheroid formation compared to that in monolayer. Ki-67 showed a remarkable increase in the spheroid formed with 2.0?×?105 cells/drop as compared to that in the monolayer. Expression levels of angiogenesis-inducing factors such as VEGF, IL-6, IL-8, and IL-18 were significantly increased in spheroid formation compared to those in the monolayer. Expression levels of bone morphogenesis-inducing factors such as Cox-2 and TGF-β1 were also significantly increased in spheroid formation compared to those in the monolayer. Expression levels of osteocyte-specific markers such as RUNX2, osteocalcin, and differentiation potential were also significantly increased in spheroid formation compared to those in the monolayer. Therefore, spheroid formation of eAD-MSCs through the hanging drop method can increases the expression of angiogenesis-inducing and bone morphogenesis-inducing factors under optimal culture conditions. 相似文献
73.
Berta Caballero-López José M. Blanco-Moreno Nicolás Pérez-Hidalgo José M. Michelena-Saval Juli Pujade-Villar Emilio Guerrieri José A. Sánchez-Espigares F. Xavier Sans 《Journal of pest science》2012,85(1):81-88
The aphid–natural enemy interaction in winter wheat fields constitutes a complex system that has been frequently studied because
of its implication for biological control. However, not all of the aphids living in cereal fields are crop pests, as there
are also aphids living on weeds that may serve as alternative hosts or prey for aphid parasitoids or predators. In this context,
a concomitant survey of the plant and insect communities was conducted to understand how different plant communities affect
the abundance and richness of aphids and the interactions with their natural enemies. The plant community was split into functional
groups (grasses, legumes and forbs), and the aphid community was divided into feeding groups according to their host preferences
(specialists in grasses or forbs). The grass aphids, which dominated the total aphid catches, responded positively to grass
cover, which was particularly enhanced in the conventional fields. Conversely, the forb aphids, which mainly conditioned the
total species richness of the aphids, were closely correlated with the local abundance of legumes. The system of cereal aphid-parasitoids
was enhanced in the conventional fields, where the abundance of grasses was higher, whereas the legumes of the organic fields
indirectly played a key role in enhancing the richness of the parasitoids and the abundance of predators. Our findings indicate
that a bottom-up effect exists throughout the plant community, aphids, and aphidophagous insects and that plant community
characteristics should be considered to better understand cereal aphid control. 相似文献
74.
Zinnat A. Begum Ismail M. M. Rahman Hikaru Sawai Satoshi Mizutani Teruya Maki Hiroshi Hasegawa 《Water, air, and soil pollution》2013,224(3):1-21
Development of aminopolycarboxylate chelants (APCs) having enhanced biodegradability is gaining increasing focus to replace the EDTA and its homologs with those used widely for the ex situ treatment of contaminated soils and are potential eco-threats. The paper reports the chelant-assisted extraction of the toxic metals (Cd, Cu, Pb, and Zn) from the metal-spiked European reference soils (Eurosoil 1 and Eurosoil 4) using biodegradable APCs, namely EDDS, GLDA, and HIDS. The effects of chelant-to-metal molar ratio, solution pH, and metal/chelant stability constants were evaluated, and compared with that of EDTA. The selectivity aptitude of the biodegradable chelants towards the toxic metals was assumed from the speciation calculations, and a proportionate correlation was observed at neutral pH. Pre- and post-extractive solid phase distributions of the target metals were defined using the sequential extraction procedure and dissolution of metals from the theoretically immobilized fraction was witnessed. The effect of competing species (Al, Ca, Fe, Mg, and Mn) concentrations was proven to be minimized with an excess of chelant in solution. The highlight of the outcomes is the superior decontamination ability of GLDA, a biodegradable APC, at minimum chelant concentration in solution and applicability at a wide range of pH environments. 相似文献
75.
Context
Spatial variation in abundance is influenced by local- and landscape-level environmental variables, but modeling landscape effects is challenging because the spatial scales of the relationships are unknown. Current approaches involve buffering survey locations with polygons of various sizes and using model selection to identify the best scale. The buffering approach does not acknowledge that the influence of surrounding landscape features should diminish with distance, and it does not yield an estimate of the unknown scale parameters.Objectives
The purpose of this paper is to present an approach that allows for statistical inference about the scales at which landscape variables affect abundance.Methods
Our method uses smoothing kernels to average landscape variables around focal sites and uses maximum likelihood to estimate the scale parameters of the kernels and the effects of the smoothed variables on abundance. We assessed model performance using a simulation study and an avian point count dataset.Results
The simulation study demonstrated that estimators are unbiased and produce correct confidence interval coverage except in the rare case in which there is little spatial autocorrelation in the landscape variable. Canada warbler abundance was more highly correlated with site-level measures of NDVI than landscape-level NDVI, but the reverse was true for elevation. Canada warbler abundance was highest when elevation in the surrounding landscape, defined by an estimated Gaussian kernel, was between 1300 and 1400 m.Conclusions
Our method provides a rigorous way of formally estimating the scales at which landscape variables affect abundance, and it can be embedded within most classes of statistical models.76.
Yukinori Mukai Audrey Daning Tuzan Leong Seng Lim Syahirah Yahaya 《Fisheries Science》2010,76(3):457-461
Sutchi catfish Pangasianodon hypophthalmus hatch with morphologically immature sensory organs; however, sensory organs develop rapidly with larval growth. Two-day-old
larvae commenced ingesting Artemia nauplii. The larvae displayed many taste buds on the barbels, the head surface, and in the buccal cavity. Other sense organs
were also well developed at this stage. Feeding experiments revealed that 2-day-old larvae ingested Artemia under both light and dark conditions, moreover, the larvae could ingest frozen dead Artemia. The ingestion rates for 4- and 7-day-old larvae were significantly higher under dark conditions than under light conditions.
The rates using frozen dead Artemia were mostly higher than the rates using live Artemia. Therefore, feeding behavior under dark conditions is most likely not mediated by visual or mechanical senses, but rather
by chemosensory senses, such as taste buds. Larval fish are vulnerable to predators; thus, if they can search for and eat
food at night, they can avoid diurnal predators. The behavior observed here appears to represent their survival strategy.
Moreover, these results suggest a new possibility that sutchi catfish larvae can be reared under dark or dim light conditions
in order to improve survival and growth rates as in the case of African catfish Clarias gariepinus. 相似文献
77.
A NMR study of water distribution in hardwoods at several equilibrium moisture contents 总被引:1,自引:0,他引:1
The water state of one tropical (Robinia coccinea) and two temperate (Acer saccharum and Fagus grandifolia) hardwoods was determined at different equilibrium moisture contents (EMC) during desorption at 25°C. NMR technique was used
to separate different components of water in wood. The species studied presented different structures, which were apparent
on the spin–spin relaxation T2 values. Three different water components were separated: slow T2 (liquid water in vessel elements), medium T2 (liquid water in fiber and parenchyma elements) and fast T2 (bound or cell wall water). The NMR results showed that even at equilibrated conditions a region exists where loss of liquid
water and bound water takes place simultaneously. This region will vary according to the wood structure. Finally, liquid water
was present at EMC lower than the fiber saturation point, which contradicts the concept of this point when considered as a
bulk property of wood. 相似文献
78.
Tradable biodiversity credit systems provide flexible means to resolve conflicts between development and conservation land-use
options for habitats occupied by threatened or endangered species. We describe an approach to incorporate the influence of
habitat fragmentation into the conservation value of tradable credits. Habitat fragmentation decreases gene flow, increases
rates of genetic drift and inbreeding, and increases probabilities of patch extinction. Importantly, tradable credit systems
will change the level of fragmentation over time for small and/or declining populations. We apply landscape equivalency analysis
(LEA), a generalizable, landscape-scale accounting system that assigns conservation value to habitat patches based on patch
contributions to abundance and genetic variance at landscape scales. By evaluating habitat trades using two models that vary
the relationship between dispersal behaviors and landscape patterns, we show that LEA provides a novel method for limiting
access to habitat at the landscape-scale, recognizing that the appropriate amount of migration needed to supplement patch
recruitment and to offset drift and inbreeding will vary as landscape pattern changes over time. We also found that decisions
based on probabilities of persistence alone would ignore changes in migration, genetic drift, and patch extinction that result
from habitat trades. The general principle of LEA is that habitat patches traded should make at least equivalent contributions
to rates of recruitment and migration estimated at a landscape scale. Traditional approaches for assessing the “take” and
“jeopardy” standards under the Endangered Species Act based on changes in abundance and probability of persistence may be
inadequate to prevent trades that increase fragmentation. 相似文献
79.
Nuno Figueiredo Corina Carranca Henrique Trindade José Pereira Piebiep Goufo João Coutinho Paula Marques Rosa Maricato Amarilis de Varennes 《Paddy and Water Environment》2015,13(4):313-324
The present field experiment was conducted during two consecutive cropping seasons in central Portugal to study the effects of simultaneous elevation of carbon dioxide concentration ([CO2]) (550 μmol mol?1) and air temperature (+2–3 °C) on japonica rice (Oryza sativa L. “Ariete”) yield, crop duration, and SPAD-values across the seasons compared with the open-field condition. Open-top chambers were used in the field to assess the effect of elevated air temperature alone or the combined effect of elevated air temperature and atmospheric [CO2]. Open-field condition was assessed with randomized plots under ambient air temperature and actual atmospheric [CO2] (average 382 μmol mol?1). Results obtained showed that the rice “Ariete” had a moderate high yielding under open-field condition, but was susceptible to air temperature rise of +2–3 °C under controlled conditions resulting in reduction of grain yield. The combined increase of atmospheric [CO2] with elevated air temperature compensated for the negative effect of temperature rise alone and crop yield was higher than in the open-field. SPAD-readings at reproductive stage explained by more than 60 % variation the straw dry matter, but this finding requires further studies for consolidation. It can be concluded that potential increase in air temperature may limit rice yield in the near future under Mediterranean areas where climate change scenario poses a serious threat, but long term field experiments are required. 相似文献
80.
Kentaro Honda Haruka Kagiwada Nobuyuki Takahashi Kazushi Miyashita 《Ecology of Freshwater Fish》2012,21(4):640-657
Behavioural tracking of endangered adult Sakhalin taimen (Parahucho perryi) by acoustic telemetry was conducted in the Bekanbeushi River system that flows through eastern Hokkaido in 2008–2010. A total of 39 tracked P. perryi showed extensive use of the river system, both upstream and downstream, regardless of the month or year. No tracked adult P. perryi stayed in the ocean for more than 1 day. Some of the fish (20%) dwelled in upstream habitat consistently from spring to autumn, while 40% of individuals showed wide utilisation of the entire upstream and downstream systems. Although 39.4% of tracked fish utilised two or more primary tributaries to the main river, 6.1% used only the main river system. Distinct variability was observed between individuals based on the proportion of time spent in each stream reach (up‐, mid‐, and downstream). A trend was observed in 2008 and 2010 showing that tracked fish moved to the upper stream reaches when water temperatures downstream increased. This trend was not observed in 2009 when the summer water temperature downstream was more than 2 °C below average when compared to 2008 and 2010. Our results suggest that adult P. perryi in the Bekanbeushi River system exhibit high behavioural plasticity, that is, the fish can select habitats based on their own individual requirements. Our results also demonstrate the importance of physical continuity among river reaches both upstream and downstream such that P. perryi does not experience barriers in moving to optimal habitats. 相似文献