全文获取类型
收费全文 | 11833篇 |
免费 | 3553篇 |
国内免费 | 38篇 |
专业分类
林业 | 459篇 |
农学 | 716篇 |
基础科学 | 45篇 |
2301篇 | |
综合类 | 756篇 |
农作物 | 725篇 |
水产渔业 | 2832篇 |
畜牧兽医 | 5943篇 |
园艺 | 84篇 |
植物保护 | 1563篇 |
出版年
2023年 | 32篇 |
2022年 | 113篇 |
2021年 | 257篇 |
2020年 | 564篇 |
2019年 | 1125篇 |
2018年 | 1012篇 |
2017年 | 1062篇 |
2016年 | 1080篇 |
2015年 | 963篇 |
2014年 | 989篇 |
2013年 | 1228篇 |
2012年 | 804篇 |
2011年 | 892篇 |
2010年 | 823篇 |
2009年 | 479篇 |
2008年 | 548篇 |
2007年 | 394篇 |
2006年 | 387篇 |
2005年 | 337篇 |
2004年 | 328篇 |
2003年 | 278篇 |
2002年 | 309篇 |
2001年 | 223篇 |
2000年 | 232篇 |
1999年 | 95篇 |
1998年 | 41篇 |
1997年 | 32篇 |
1996年 | 35篇 |
1995年 | 28篇 |
1994年 | 21篇 |
1993年 | 33篇 |
1992年 | 31篇 |
1991年 | 29篇 |
1990年 | 41篇 |
1989年 | 40篇 |
1988年 | 34篇 |
1987年 | 41篇 |
1986年 | 20篇 |
1985年 | 33篇 |
1984年 | 24篇 |
1983年 | 29篇 |
1981年 | 20篇 |
1978年 | 27篇 |
1977年 | 24篇 |
1976年 | 29篇 |
1975年 | 17篇 |
1974年 | 23篇 |
1973年 | 23篇 |
1972年 | 22篇 |
1970年 | 17篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
111.
Association of Obesity with Serum Leptin,Adiponectin, and Serotonin and Gut Microflora in Beagle Dogs 下载免费PDF全文
H.‐J. Park S.‐E. Lee H.‐B. Kim R.E. Isaacson K.‐W. Seo K.‐H. Song 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2015,29(1):43-50
Background
Serotonin (5‐hydroxytryptamine, 5HT) is involved in hypothalamic regulation of energy consumption. Also, the gut microbiome can influence neuronal signaling to the brain through vagal afferent neurons. Therefore, serotonin concentrations in the central nervous system and the composition of the microbiota can be related to obesity.Objective
To examine adipokine, and, serotonin concentrations, and the gut microbiota in lean dogs and dogs with experimentally induced obesity.Animals
Fourteen healthy Beagle dogs were used in this study.Methods
Seven Beagle dogs in the obese group were fed commercial food ad libitum, over a period of 6 months to increase their weight and seven Beagle dogs in lean group were fed a restricted amount of the same diet to maintain optimal body condition over a period of 6 months. Peripheral leptin, adiponectin, 5HT, and cerebrospinal fluid (CSF‐5HT) levels were measured by ELISA. Fecal samples were collected in lean and obese groups 6 months after obesity was induced. Targeted pyrosequencing of the 16S rRNA gene was performed using a Genome Sequencer FLX plus system.Results
Leptin concentrations were higher in the obese group (1.98 ± 1.00) compared to those of the lean group (1.12 ± 0.07, P = .025). Adiponectin and 5‐hydroytryptamine of cerebrospinal fluid (CSF‐5HT) concentrations were higher in the lean group (27.1 ± 7.28) than in the obese group (14.4 ± 5.40, P = .018). Analysis of the microbiome revealed that the diversity of the microbial community was lower in the obese group. Microbes from the phylum Firmicutes (85%) were predominant group in the gut microbiota of lean dogs. However, bacteria from the phylum Proteobacteria (76%) were the predominant group in the gut microbiota of dogs in the obese group.Conclusions and Clinical Importance
Decreased 5HT levels in obese group might increase the risk of obesity because of increased appetite. Microflora enriched with gram‐negative might be related with chronic inflammation status in obese dogs. 相似文献112.
113.
采用SP法检测商品蛋鸡感染J亚群禽白血病病毒对产蛋性能的影响 总被引:6,自引:0,他引:6
本研究属世界上首次报道J亚群禽白血病病毒感染对蛋鸡产蛋性能的影响。尸体剖检观察到产蛋鸡的生殖系统发育不良,表现为卵巢、输卵管幼稚型,输卵管粗细不均。组织病理学观察,发现卵巢组织中有大量的胞浆内充满球形嗜酸性颗粒的骨髓瘤细胞。用特异性抗J亚群禽白血病病毒(ALV—J)囊膜糖蛋白gp85的单克隆抗体检测不产蛋鸡的卵巢、输卵管等待检的组织切片,均检出病毒阳性抗原。结果表明ALV—J的感染造成产蛋鸡卵巢、输卵管发育不良,是蛋鸡不产蛋的直接原因。 相似文献
114.
Comparative studies of ionic composition, osmolality, protein concentration and pH of seminal plasma along with spermatozoa concentrations were carried out in stellate sturgeon, Acipenser stellatus, and Russian sturgeon, Acipenser gueldenstaedtii. Analysis of A. gueldenstaedtii sperm showed significantly higher concentrations of Na+ (34.58 ± 4.61 mm ), Ca2+ (0.35 ± 0.12 mm ), Mg2+ (0.70 ± 0.25 mm ), Cl? (13.50 ± 4.04 mm ) and proteins (0.60 ± 0.29 mg/ml) in the seminal plasma than did seminal plasma of A. stellatus: Na+ (20.08 ± 10.75 mm ), Ca2+ (0.28 ± 0.06 mm ), Mg2+ (0.29 ± 0.05 mm ), Cl? (7.50 ± 3.00 mm ) and 0.30 ± 0.11 mg/ml proteins. Significantly higher concentration of K+ (5.42 ± 1.06 mm ) was observed in A. stellatus compared to A. gueldenstaedtii K+ (2.29 ± 0.50 mm ). Concentration of Na+ was positively correlated with osmolality (r = 0.819), levels of Cl? (r = 0.922) and Mg2+ (r = 0.727) and pH (r = 0.848). The concentration of Mg2+ was positively correlated with protein concentration (r = 0.774), Na+ (r = 0.727), Cl? (r = 0.872) and Ca2+ (r = 0.801). A positive relationship was also found between concentration of K+ and spermatozoa concentration (r = 0.709). Results revealed strong inter‐species differences in several parameters. The data should be useful for artificial fertilization and for cryopreservation of sturgeon sperm. 相似文献
115.
Nanoemulsion from essential oil of Pterodon emarginatus (Fabaceae) shows in vitro efficacy against monogeneans of Colossoma macropomum (Pisces: Serrasalmidae) 下载免费PDF全文
D S S Valentim J L Duarte A E M F M Oliveira R A S Cruz J C T Carvalho E C Conceição C P Fernandes M Tavares‐Dias 《Journal of fish diseases》2018,41(3):443-449
Outbreaks of diseases pose a major threat to sustainable aquaculture development worldwide. Application of herbal products to combat parasitic diseases provides an alternative approach for sustainable aquaculture. This study investigated the in vitro antiparasitic effects of an oil‐in‐water nanoemulsion prepared using the essential oil from Pterodon emarginatus, against monogeneans infesting Colossoma macropomum. Gill arches from C. macropomum (47.6 ± 14.5 g and 13.5 ± 1.4 cm) that were naturally parasitized by Anacanthorus spathulatus, Notozothecium janauachensis and Mymarothecium boegeri were immersed in different dispersions of the P. emarginatus nanoemulsions (0, 50, 100, 200, 400 and 600 mg/L). The major compounds presented in the essential oil of P. emarginatus were β‐elemene, β‐caryophyllene and α‐humulene. Characterization of these nanoemulsions showed that they have a small mean droplet size and low polydispersity index, which is concordant with stable systems. In this in vitro trial, the P. emarginatus nanoemulsion concentrations of 100, 200, 400 and 600 mg/L presented 100% helminthic efficacy against monogeneans of the gills of C. macropomum. The highest two concentrations used (400 and 600 mg/L) were seen to immobilize the parasites after only 15 min. Therefore, it would be worthwhile testing these concentrations in therapeutic baths against monogeneans of C. macropomum. 相似文献
116.
117.
César Lodeiros Lee Galindo John H. Himmelman 《Aquaculture (Amsterdam, Netherlands)》2007,262(1):168-171
We ran an experiment on mangrove oysters Crassostrea rhizophorae to evaluate the effects of adding different masses of artificial fouling to the upper valve, either to the umbo region or the ventral edge of the shell. Growth and survival were quantified after a 30 d period in suspended culture in the La Restinga Lagoon, Venezuela. The artificial fouling was cement weighing 0.5, 1, 2 or 3 fold the mass of the upper valve. No fouling was added to a control group. Fouling mass, but not the position of the artificial fouling, affected growth in shell length. However, only the heaviest fouling (3 times the mass of the upper valve) had a significant effect. In contrast, there was no affect of either fouling mass or position on tissue growth. Finally, our data indicated that mortality could be affected by the position where we added artificial fouling (greater mortality when fouling was added to the ventral edge of the shell), but not by fouling mass. Our study indicates it is unlikely that the levels of natural fouling that develop on oysters in suspended culture would be sufficient to affect either growth or survival. 相似文献
118.
通过统计苦瓜白粉病的病情指数,研究白粉病抗性的遗传规律。采用主基因+多基因混合遗传分离分析法对抗病品系04-17-3和感病品系25-1杂交组合的P1、P2、F1、F2、B1和B2共6个世代群体抗白粉病遗传进行研究。结果表明:苦瓜对白粉病的抗性遗传符合2对加性-显性-上位性主基因+加性-显性多基因模型(E-1),抗病对感病为不完全隐性;2对主基因的加性效应值均为-12.00;2对主基因分别具有正向部分显性和正向超显性作用,加性效应值均大于其显性效应值,上位性效应值(i+jab+jba+l)为负值。从遗传率上看,回交世代和F2的主基因的值分别为55.14%、43.56%和95.22%,多基因的值分别为16.10%、26.57%和0,环境变异在4.78%~29.87%间。主基因和多基因共同决定了苦瓜对白粉病的抗性,以主基因遗传为主,同时还受到环境变异的部分影响。在白粉病抗性育种过程中,应注意利用加性效应,选用白粉病抗性基因较多的材料作为亲本,并在早代进行选择,尤其是F2代主基因选择效率最高。 相似文献
119.
Analgesic efficacy of an oral transmucosal spray formulation of meloxicam alone or in combination with tramadol in cats with naturally occurring osteoarthritis 下载免费PDF全文
120.
Successful rearing of whiteleg shrimp Litopenaeus vannamei larvae fed a desiccation‐tolerant nematode to replace Artemia 下载免费PDF全文
L.H. Seychelles S. Happe E. Palacios M. Ludwig S. Hollmer R.‐U. Ehlers C. Schulz L. Mercier 《Aquaculture Nutrition》2018,24(2):903-910
The nematode Panagrolaimus sp. was tested as live feed to replace Artemia nauplii during first larval stages of whiteleg shrimp Litopenaeus vannamei. In Trial 1, shrimp larvae were fed one of four diets from Zoea 2 to Postlarva 1 (PL1): (A) Artemia nauplii, control treatment; (NC) nematodes enriched in docosahexaenoic acid (DHA) provided by the dinoflagellate Crypthecodinium cohnii; (N) non‐enriched nematodes; and (Algae) a mixture of microalgae supplemented in C. cohnii cells. In Trial 2, shrimp were fed (A), (NC) and a different treatment (NS) with nematodes enriched in polyunsaturated fatty acids (PUFAs) provided by the commercial product S.presso®, until Postlarva 6 (PL6). Mysis 1 larvae fed nematodes of the three dietary treatments were 300 μm longer (3.2 ± 0.3 mm) than control larvae. At PL1, control shrimp were 300 μm longer (4.5 ± 0.3 mm) than those fed DHA‐enriched or PUFAs‐enriched nematodes. No differences were observed in length and survival at PL6 between control larvae and those fed DHA‐enriched nematodes (5.1 ± 0.5 mm; 33.1%–44.4%). Shrimp fed microalgae showed a delay in development at PL1. This work is the first demonstration of Panagrolaimus sp. suitability as a complete substitute for Artemia in rearing shrimp from Zoea 2 to PL6. 相似文献