全文获取类型
收费全文 | 225篇 |
免费 | 12篇 |
国内免费 | 2篇 |
专业分类
林业 | 16篇 |
农学 | 6篇 |
基础科学 | 7篇 |
51篇 | |
综合类 | 28篇 |
农作物 | 16篇 |
水产渔业 | 17篇 |
畜牧兽医 | 77篇 |
园艺 | 9篇 |
植物保护 | 12篇 |
出版年
2024年 | 1篇 |
2023年 | 1篇 |
2022年 | 6篇 |
2021年 | 10篇 |
2020年 | 10篇 |
2019年 | 7篇 |
2018年 | 7篇 |
2017年 | 6篇 |
2016年 | 6篇 |
2015年 | 10篇 |
2014年 | 3篇 |
2013年 | 8篇 |
2012年 | 14篇 |
2011年 | 23篇 |
2010年 | 10篇 |
2009年 | 13篇 |
2008年 | 19篇 |
2007年 | 11篇 |
2006年 | 17篇 |
2005年 | 11篇 |
2004年 | 8篇 |
2003年 | 3篇 |
2002年 | 4篇 |
2001年 | 1篇 |
2000年 | 4篇 |
1998年 | 4篇 |
1997年 | 2篇 |
1996年 | 3篇 |
1995年 | 2篇 |
1993年 | 1篇 |
1991年 | 2篇 |
1990年 | 2篇 |
1987年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 2篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1967年 | 1篇 |
1953年 | 1篇 |
1930年 | 1篇 |
排序方式: 共有239条查询结果,搜索用时 31 毫秒
11.
Tim J Bull Christina Vrettou Richard Linedale Catherine McGuinnes Sam Strain Jim McNair Sarah C Gilbert Jayne C Hope 《Veterinary research》2014,45(1)
Vaccination is the most cost effective control measure for Johne’s disease caused by Mycobacterium avium subspecies paratuberculosis (MAP) but currently available whole cell killed formulations have limited efficacy and are incompatible with the diagnosis of bovine tuberculosis by tuberculin skin test. We have evaluated the utility of a viral delivery regimen of non-replicative human Adenovirus 5 and Modified Vaccinia virus Ankara recombinant for early entry MAP specific antigens (HAV) to show protection against challenge in a calf model and extensively screened for differential immunological markers associated with protection. We have shown that HAV vaccination was well tolerated, could be detected using a differentiation of infected and vaccinated animals (DIVA) test, showed no cross-reactivity with tuberculin and provided a degree of protection against challenge evidenced by a lack of faecal shedding in vaccinated animals that persisted throughout the 7 month infection period. Calves given HAV vaccination had significant priming and boosting of MAP derived antigen (PPD-J) specific CD4+, CD8+ IFN-γ producing T-cell populations and, upon challenge, developed early specific Th17 related immune responses, enhanced IFN-γ responses and retained a high MAP killing capacity in blood. During later phases post MAP challenge, PPD-J antigen specific IFN-γ and Th17 responses in HAV vaccinated animals corresponded with improvements in peripheral bacteraemia. By contrast a lack of IFN-γ, induction of FoxP3+ T cells and increased IL-1β and IL-10 secretion were indicative of progressive infection in Sham vaccinated animals. We conclude that HAV vaccination shows excellent promise as a new tool for improving control of MAP infection in cattle.
Electronic supplementary material
The online version of this article (doi:10.1186/s13567-014-0112-9) contains supplementary material, which is available to authorized users. 相似文献12.
- 1. Top predators such as crocodiles often reflect ecosystem degradation. The recent spate of close to 200 Nile crocodile deaths may reflect the ecosystem state of the Olifants–Letaba River system in the Kruger National Park. This paper investigates whether the crocodile deaths were indeed the consequence of a rare and perhaps acute event given the variability in annual population growth rates of crocodilians.
- 2. Spotlight‐ and helicopter‐based surveys designed to correct for availability and detectability bias were used to estimate population sizes of crocodiles from 2008 to 2010 in and around the Olifants River Gorge, the focal area of crocodile deaths.
- 3. Correction factors derived from individually observed crocodiles for both spotlight‐ and helicopter‐based counts were lower than those typically used for Nile crocodiles as well as other crocodile species. Even so, corrected spotlight‐ and helicopter‐based estimates were comparable and the number of crocodiles in the focal study area declined significantly from 780 (95% CI: 637–1222) to between 460 (spotlight estimate, 95% CI 375–665) and 505 (aerial estimate, 95% CI: 559–1746) during the period of crocodile deaths. The average annual decline of 35% was at the lower end of the distribution of annual population growth rates across the rivers of Kruger National Park.
- 4. The crocodile deaths reflect a possible rare event that suggests a degraded crocodile population, possibly the consequences of broad‐scale cascades of environmental deterioration of the Olifants–Letaba River system.
- 5. Even so, the potential risk of local demise of the population in the focal study area in the short term may be diminished through evolutionary, demographic and spatial resilience inherent within crocodiles that can accommodate the as yet unknown disease dynamics of pansteatitis. Copyright © 2011 John Wiley & Sons, Ltd.
13.
Jerom R. Stocks Sam Davis Marti J. Anderson Martin W. Asmus Katherine J.M. Cheshire Dylan E. van der Meulen Chris T. Walsh Dean M. Gilligan 《水产资源保护:海洋与淡水生态系统》2021,31(11):3228-3247
- Fish assemblages in dryland rivers have life-history strategies that have evolved in response to environmental conditions and triggers, particularly water temperatures and flow regimes. The regulation of rivers through the construction of dams, weirs and other water diversion structures has altered natural flow regimes and the associated ecological processes of river systems worldwide.
- Over a 3-year period, using standardized fish sampling and daily otolith ageing, the recruitment of eight freshwater fish species was monitored in response to various abiotic drivers, including hydrology and water temperatures, throughout the Macquarie River, a large regulated river system of the Australian Murray-Darling Basin.
- A data-driven statistical classification system is provided that groups species into reproductive guilds, based on their recruitment response to hydrology and water temperature, specifically designed for use in environmental flow management.
- The eight species were grouped into three distinct reproductive guilds that showed similar recruitment responses to the abiotic drivers. Murray-Darling rainbowfish, Murray cod, and eel-tailed catfish were considered as a single guild, characterized by seasonal recruitment during a relatively narrow thermal window under low to moderate stable discharges. The second group included common carp, bony herring, and golden perch, which recruited primarily in association with larger flow events. Un-specked hardyhead and Australian smelt formed a more differentiated guild, recruiting over a broad range of temperatures and discharges. Limitations associated with using a reproductive guild approach to simplify water management are discussed.
- This study highlighted important relationships among hydrology, water temperatures, and successful recruitment that can, in turn, be used to inform development of adaptive flow management plans and effective use of environmental water for the conservation management of native fish communities. Important considerations in the design of studies that aim to examine relationships between recruitment and abiotic drivers are also discussed.
14.
Yvonne Lokko Alfred Dixon Sam Offei Eric Danquah Martin Fregene 《Genetic Resources and Crop Evolution》2006,53(7):1441-1453
A study was conducted to determine the extent of genetic diversity among African cassava (Manihot esculenta Crantz) accessions resistant to the cassava mosaic virus disease (CMD), using simple sequence repeat (SSR) markers. The accessions
included a breeding stock (clone 58308), five improved lines, 62 CMD resistant and 10 CMD susceptible landraces. Genetic diversity
was assessed among accessions in five cluster groups derived from UPGMA analysis on data from 18 SSR primer pairs. Average
gene diversity, He, was high in all cluster groups, with an average heterozygosity of 0.591 ± 0.061. The estimator of inbreeding
Fis revealed a low level of inbreeding within groups and averaged −0.262 ± 0.142. Gene diversity among all accessions was
51.4% and gene diversity within cluster groups was 46.6%, while 4.8% was due to diversity between the different cluster groups.
The amount of genetic differentiation measured by Gst and Fst were 9.6% and 12.1% respectively, indicating a weak genetic
structure. 相似文献
15.
Gilbert SJ Dumontheil I Simons JS Frith CD Burgess PW 《Science (New York, N.Y.)》2007,317(5834):43; author reply 43
Mason et al. (Reports, 19 January 2007, p. 393) attributed activity in certain regions of the "resting" brain to the occurrence of mind-wandering. However, previous research has demonstrated the difficulty of distinguishing this type of stimulus-independent thought from stimulus-oriented thought (e.g., watchfulness). Consideration of both possibilities is required to resolve this ambiguity. 相似文献
16.
Zimbabwe's communal farmers are now less food secure than they were two generations ago. The roots of this decline lie not only in the confinement of Africans to marginal land but also in the historic forced replacement of their sustainable, indigenous farming system with one whose productivity now relies on the use of large amounts of expensive chemical inputs. Environmentally-friendly, traditional farming practices such as pyro-culture, minimum tillage, mixed cropping, and bush fallowing were completely wiped out and replaced with a highly technical western or ganic farming system based on plough cultivation and continuous monocultures of commodity crops, that were supposed to be sustained by liberal amounts of green and animal manures. This gave rise to an effective agricultural hegemony that was due, mainly, to the zealous dedication of an American Mormon missionary whose motives were evangelical rather than scientific. 相似文献
17.
18.
Clive A. Henrick Barbara A. Garcia Gerardus B. Staal David C. Cerf Richard J. Anderson Karen Gill Henry R. Chinn Jeffrey N. Labovitz Michael M. Leippe Sam L. Woo Robert L. Carney Douglas C. Gordon Gustave K. Kohn 《Pest management science》1980,11(2):224-241
A new series of substituted 2-anilino-3-methylbutyrates has been prepared; bioassay data for these compounds on Heliothis virescens, Musca domestica, Aphis fabae and Tetranychus urticae are presented and discussed. Some unexpected relationships were observed between the nature of the substituents and the biological activity. Increases in foliar stability were noted with certain substitution patterns. Both α-cyano-3-phenoxybenzyl 3-methyl-2-(α, α, α,2-tetrafluoro-p-toluidino)butyrate and the corresponding 2-(2-chloro-α, α, α-trifluoro-p-toluidino)-3-methylbutyrate showed good stability in air and light, and exhibited biological activities of a similar nature and potency to those of previously known synthetic pyrethroids. Esters of the (R)-2- anilino-3-methylbutyric acids are far more active than those prepared from the (S)-enantiomers. The (R)-configuration at C-2 in these acids is sterically equivalent to the active absolute configuration at the chiral carbon α to the carboxylate group in both the permethrin and the fenvalerate types of pyrethroids. A new class of insecticidal 2-(isoindolin-2-yl)alkanoates is also reported. In this series the most biologically active analogue was α-cyano-3-phenoxybenzyl 3-methyl-2-(4,5,6,7-tetrafluoroisoindolin-2-yl)butyrate. These esters were considerably less stable than the anilino analogues on exposure to air and light. 相似文献
19.
Survey radiographs have not accurately identified renal contour or location in nonhuman primates. Excretory urography using 786 to 1193 mg iodinelkg body weight resulted in dense opacification of the renal parenchyma and pelvis. Abdominal compression improved visu- alization of the renal pelvis. Interpretation of the nonhuman primate excretory urogram was compromised by several anatomic characteristics of this animal group. Superimposition of the kidneys on the lateral radiograph limited evaluation of the renal contour. Since the renal pelvis in most species of nonhuman primates does not possess diverticula (as in the dog and cat) or a caliceal system (as in the pig and man), the diagnosis of pyelonephritis or renal mass lesions was difficult. Severe irregularities in the renal contour, size of the renal pelvis, or areas of deficient concentrating ability were identifiable in nonhuman primate excretory urograms, but the diagnosis of small cysts was not possible in smaller nonhuman primates. The presence of a caliceal collecting system in the spider monkey (Ateles sp.) suggests this animal as a potential model for the evaluation of human renal disease. 相似文献
20.
Camacho AT Guitian EJ Pallas E Gestal JJ Olmeda AS Goethert HK Telford SR Spielman A 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2004,18(2):141-146
Babesia microti-like piroplasms are a recently recognized cause of illness in dogs in northwest Spain. Our objective was to describe the clinical characteristics and investigate the risk factors for azotemia and death among 58 B microti-like infected dogs. Twenty-one of the 58 (36%) dogs were azotemic at the time that the infection was diagnosed. The case fatality rate during the following week was 22%. Dogs with azotemia at the time of diagnosis were 10 times (95% CI, 3.26-28.8) more likely to die during the following week. Azotemia was the main cause of death for B microti-like infected dogs (attributable fraction = 90%). Severe anemia was present in 45 of the 58 (78%) dogs. Azotemic dogs also presented with hyperphosphatemia, hypoalbuminemia, hypercholesterolemia, proteinuria, and high urine protein: creatinine ratios, suggesting a glomerular component to the disease. Age was the only factor significantly associated with the risk of azotemia (P = .042): on average, a 4-year age increase doubled the risk of an infected dog being azotemic. The only factor significantly associated with mortality was azotemia (P = .001). We concluded that B microti-like infection is associated with a high risk of azotemia and mortality. 相似文献