Modelling Impacts of Atmospheric Deposition,Nutrient Cycling and Soil Weathering on the Sustainability of Nine Forest Ecosystems

To assess the impact of acid deposition on the long-term sustainability of nine oak, pine and spruce stands on sandy to loamy sandy parent material in Sweden, Denmark and The Netherlands, a dynamic soil acidification model (ReSAM) was applied. Two deposition scenarios for the period 1990–2090 were used: a business as usual scenario (BAU) and a restrictive critical load scenario (CL). The BAU scenario leads to a strong decrease in both Al concentrations and pH in the topsoil of the Dutch and the Danish sites due to a decrease in the amount of amorphous Al compounds. The decline in pH leads to an enhanced release of base cations by silicate weathering. Despite the ongoing acidification, base saturation increases during the simulation period, due to both the increase in base cation weathering and an increased input from mineralization with the ageing of the tree stands. No change in Al concentration is predicted for northern Sweden as deposition levels are below critical loads. Soil chemistry at the recently replanted Swedish sites is dominated by changes in N cycling instead of by deposition. The CL scenario leads, especially after 2010, to a stronger decline in Al concentration compared with the BAU scenario, which is mainly caused by a reduction of the acid input. Up to 2010, a considerable acid input to the soil system is maintained as N supply is larger than the consumption of N by the trees. Despite the reduction of the deposition of S and N to critical loads, the readily available cation pools are still declining on the Danish and Dutch sites in 2090. It is concluded that deposition levels above critical loads lead to exhaustion of the pool of amorphous Al compounds and a decline in pH. Base saturation does not decline due to an increase in mineralization with stand age and an increase in weathering rate due to the decline in pH.     

Field study indicating susceptibility differences between salmonid species and their lineages to proliferative kidney disease

Tetracapsuloides bryosalmonae (Myxozoa: Malacosporea) is the causative agent of proliferative kidney disease (PKD), which affects both wild and farmed salmonid fish. The objective of this study was to outline differences in susceptibility to PKD in different salmonid species, hybrids and breeding lineages. Susceptibility to T. bryosalmonae infection was established based on cumulative mortality, pathological findings and detection of T. bryosalmonae in the kidney using immunohistochemistry and molecular methods. Determination of pure and hybrid individuals of different species in the genus Salvelinus, and dissimilarity of rainbow trout lineages, was performed using traditional polymerase chain reaction (PCR) and microsatellite analyses. Rainbow trout displayed higher disease severity compared with brook trout and Alsatian charr. Moreover, the results indicated differences in infection susceptibility, not only among different salmonid species but also among different lineages of charr and rainbow trout. Our study indicated that some salmonid species and even different lineages of the same species are more suitable for farming under PKD pressure.     

Ecosystem effects of invertebrate fisheries

Since the 1950s, invertebrate fisheries catches have rapidly expanded globally to more than 10 million tonnes annually, with twice as many target species, and are now significant contributors to global seafood provision, export, trade and local livelihoods. Invertebrates play important and diverse functional roles in marine ecosystems, yet the ecosystem effects of their exploitation are poorly understood. Using 12 ecosystem models distributed worldwide, we analysed the trade‐offs of various invertebrate fisheries and their ecosystem effects as well as ecological indicators. Although less recognized for their contributions to marine food webs, our results show that the magnitude of trophic impacts of invertebrates on other species of commercial and conservation interest is comparable with those of forage fish. Generally, cephalopods showed the strongest ecosystem effects and were characterized by a strong top‐down predatory role. Lobster, and to a lesser extent, crabs, shrimp and prawns, also showed strong ecosystem effects, but at lower trophic levels. Benthic invertebrates, including epifauna and infauna, also showed considerable ecosystem effects, but with strong bottom‐up characteristics. In contrast, urchins, bivalves, and gastropods showed generally lower ecosystem effects in our simulations. Invertebrates also strongly contributed to benthic–pelagic coupling, with exploitation of benthic invertebrates impacting pelagic fishes and vice versa. Finally, on average, invertebrates produced maximum sustainable yield at lower levels of depletion (~45%) than forage fish (~65%), highlighting the need for management targets that avoid negative consequences for target species and marine ecosystems as a whole.     

Global patterns of predator diversity in the open oceans

The open oceans comprise most of the biosphere, yet patterns and trends of species diversity there are enigmatic. Here, we derive worldwide patterns of tuna and billfish diversity over the past 50 years, revealing distinct subtropical "hotspots" that appeared to hold generally for other predators and zooplankton. Diversity was positively correlated with thermal fronts and dissolved oxygen and a nonlinear function of temperature (approximately 25 degrees C optimum). Diversity declined between 10 and 50% in all oceans, a trend that coincided with increased fishing pressure, superimposed on strong El Ni?o-Southern Oscillation-driven variability across the Pacific. We conclude that predator diversity shows a predictable yet eroding pattern signaling ecosystem-wide changes linked to climate and fishing.     

Effects of infection with the ectoparasite Argulus japonicus (Thiele) and administration of cortisol on cellular proliferation and apoptosis in the epidermis of common carp,Cyprinus carpio L., skin

The host‐parasite interaction between juvenile carp, Cyprinus carpio, and the ectoparasitic branchiuran, Argulus japonicus, together with the role of cortisol in this interaction, was examined at the level of the host skin epidermis. Epidermal mucous cell numbers, and proliferation and apoptosis of the epithelial cells were studied over 32 days. Apoptotic cell numbers in the uppermost epidermis were reduced at 26 days post‐infection with A. japonicus, while the other parameters were unaffected. Administration of cortisol‐containing food resulted in reduced apoptosis in the cells in the upper skin epidermis at 24 h and at 28 days post‐feeding. Cortisol feeding combined with A. japonicus infection reduced numbers of apoptotic cells in the upper epidermis more than either individual treatment. Further, combining the treatments also significantly increased apoptosis in the lower epidermis in cells morphologically identified as leucocytes apparently migrating macrophages and lymphocytes. Using immunohistochemistry, we demonstrated cortisol receptor presence and cellular localization in the teleost epidermis. Receptors only occurred in pavement cells in the upper epidermis and in leucocytes in the lower parts of the epidermis. The ectoparasites, or administered cortisol, induced effects which may be functionally adaptive in the upper pavement cells, while combining the two treatments also induced changes indicative of immunosuppression.     

Soil Acidification in Loess and Clay Soils in The Netherlands

To assess the impact of acid deposition on forestedloess and clay soils in the Netherlands, changes inbase saturation and soil solution concentrations weresimulated with the dynamic soil acidification modelReSAM for 38 loess soils and 16 clay soils. Theselected locations represent the range in geographicalposition and diversity in parent material occurring inforested loess and clay soils in the Netherlands. Twodeposition scenarios were used for the period1992–2050: a business as usual scenario (BAU) and ascenario in which deposition was reduced according topresent Dutch policy plans (MV-3). A comparison ofsimulated and measured soil solution concentrationsand base saturation in 1992/1993 showed that the modelsimulated concentrations and base saturation in theloess soils quite good. However, the model tended tooverestimate acidification in the top (0–10 cm) of theclay soils. Despite the reasonable agreement betweenmeasured and simulated data some uncertainty in thevalidity of the model predictions remains because timetrends to validate the model were not available. Themodel predicted a small but ongoing acidificationduring the BAU scenario in the loess soils asindicated by a decline in median base saturation andpH in the topsoil in 2050. Present policy plans (MV-3)lead to a slight recovery of the base saturation in2050 and a decline in Al concentrations. In the claysoils a strong decline in base saturation is simulatedin the topsoil, whereas an increase in base saturationis predicted for the subsoil.     

Back to the roots: how do seedlings of native tree species react to the competition by exotic species?

Context

Identifying the traits of exotic species may explain their invasiveness and help control the negative impacts of these species on native ecosystems. In this study, we investigated root competition as an important driver for the competitiveness of two exotic tree species in the seedling stage.

Aims

In a pot experiment, carried out in Central Europe, we studied the effect of root competition of two exotic tree species (Prunus serotina Ehrh. and Robinia pseudoacacia L.) on biomass allocation of two native ones (Quercus robur L. and Carpinus betulus L.).

Methods

Seedlings of these species were exposed to intra- and interspecific mixtures with and without the effect of root competition. For this, belowground plastic partitions were installed in the pots.

Results

Competition intensity in the pots increased over time, irrespective of mixture type. However, this increase was much higher for the native species if mixed with the exotic species compared to monocultures or inter-native mixtures. In addition to ontogeny, competition affected biomass allocation patterns. Under root competition by the exotic species, the native species preferentially allocated their biomass to the roots. A higher allocation to the roots was mainly achieved at the expense of leaf and branch biomass.

Conclusion

Root competition of P. serotina and R. pseudoacacia may be a reason for the lack of Q. robur and C. betulus in the seedling stage in natural environments where all four species occur.     

Reciprocal rewards stabilize cooperation in the mycorrhizal symbiosis

Plants and their arbuscular mycorrhizal fungal symbionts interact in complex underground networks involving multiple partners. This increases the potential for exploitation and defection by individuals, raising the question of how partners maintain a fair, two-way transfer of resources. We manipulated cooperation in plants and fungal partners to show that plants can detect, discriminate, and reward the best fungal partners with more carbohydrates. In turn, their fungal partners enforce cooperation by increasing nutrient transfer only to those roots providing more carbohydrates. On the basis of these observations we conclude that, unlike many other mutualisms, the symbiont cannot be "enslaved." Rather, the mutualism is evolutionarily stable because control is bidirectional, and partners offering the best rate of exchange are rewarded.     

CB1 cannabinoid receptors and on-demand defense against excitotoxicity

Abnormally high spiking activity can damage neurons. Signaling systems to protect neurons from the consequences of abnormal discharge activity have been postulated. We generated conditional mutant mice that lack expression of the cannabinoid receptor type 1 in principal forebrain neurons but not in adjacent inhibitory interneurons. In mutant mice,the excitotoxin kainic acid (KA) induced excessive seizures in vivo. The threshold to KA-induced neuronal excitation in vitro was severely reduced in hippocampal pyramidal neurons of mutants. KA administration rapidly raised hippocampal levels of anandamide and induced protective mechanisms in wild-type principal hippocampal neurons. These protective mechanisms could not be triggered in mutant mice. The endogenous cannabinoid system thus provides on-demand protection against acute excitotoxicity in central nervous system neurons.     

Assessment of the Regional Variation in Weathering Rates of Loess and Clay Soils in The Netherlands

To calculate critical acid loads or to predict elementconcentrations in the soil solution, information on weatheringrates is essential. Several studies have taken place in theNetherlands to obtain weathering rates for non-calcareous sandysoils. Recently information on weathering rates in less vulnerable loess and clay soils have become available. However,up to now no system is available to estimate weathering rates ona regional scale by relating them to regionally available soilproperties.To obtain weathering rates of loess and clay soils on a regionalscale for the Netherlands, the applicability of a statisticalregression model and the process based PROFILE model have beenevaluated. Both models were calibrated on a set of laboratoryexperiments. To evaluate their predictive power, both methodswere validated on a number of sites for which field weatheringrates were available.Predictions with the statistical model, for the individual basecations, were generally within a factor 2 of the calculatedhistorical weathering rates, except for Ca, which wasoverestimated, by a factor 3 to 4. PROFILE stronglyoverestimated all weathering rates using both standard parameters and in particular after calibration on the laboratoryrates. However, PROFILE predicted weathering rates of the loesssoils quite good after calibration on historical weatheringrates, indicating that the downscaling procedure used in PROFILEto translate laboratory to field weathering rates is inadequatefor the considered soils.The statistical model was applied to predict weathering rates,for the Netherlands on a 1 × 1 km grid scale. Weathering rates at the present pH values in forested loess and clay soilsranged from 135 to 6000 mol_c ha^-1 a^-1 in loesssoils and from 100 to 1750 mol_c ha^-1 a^-1 in claysoils.