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181.
We established protocols for the analysis of genetic diversity in chayote (Sechium edule) by using isozyme markers, thereby determining the level of genetic diversity present in 42 accessions of chayote from Costa
Rica. We obtained clear and reproducible zymograms for eight enzyme staining systems: PGM, 6-PGD, PGI, IDH, MDH, SOD, SKD,
and EST, and were able to score 14 putative loci. Eight of the 14 loci examined were polymorphic. We found 35 distinct multilocus
genotypes among these accessions. Five of these multilocus genotypes were homozygous for all loci. In addition, our data also
revealed that most of the multilocus genotypes (24) were heterozygous for only one of the eight loci, and the rest were heterozygous
for two or three loci (9 and 4 accessions, respectively). Seven multilocus genotypes were found in two different accessions.
Dice similarity coefficient was used to study the relationship between accessions. This analysis, based on the presence and
absence of alleles, revealed that accessions collected in the same location seldom shared the same multilocus genotype. The
value of isozyme polymorphisms as tools to continue studies on the characterization of chayote is discussed. 相似文献
182.
Influence of non-cellulose structural carbohydrate composition on plant material decomposition in soil 总被引:1,自引:0,他引:1
Sophie Gunnarsson Håkan Marstorp A Sigrun Dahlin Ernst Witter 《Biology and Fertility of Soils》2008,45(1):27-36
The C mineralisation pattern during the early stage of decomposition of plant materials is largely determined by their content
of different carbohydrates. This study investigated whether detailed plant analysis could provide a better prediction of C
mineralisation during decomposition than proximate analysis [neutral detergent solution (NDF)/acid detergent solution (ADF)].
The detailed analysis included sugars, fructans, starch, pectin, cellulose, lignin and organic N. To determine whether differences
in decomposition rate were related to differences in hemicellulose composition, the analysis particularly emphasised the concentrations
of arabinose and xylose in hemicelluloses. Carbon dioxide evolution was monitored hourly in soil amended with ten different
plant materials. Principal component and regression analysis showed that C mineralisation during day 1 was closely related
to free sugars, fructans and soluble organic N components (R
2 = 0.83). The sum of non-cellulose structural carbohydrates (intermediate NDF/ADF fraction) was not related to C mineralisation
between days 1 and 9. In contrast, a model including starch and protein in addition to the non-cellulose structural carbohydrates,
with the hemicelluloses replaced by arabinose and xylose, showed a strong relationship with evolved CO2 (R
2 = 0.87). Carbon mineralisation between days 9 and 34 was better explained by xylan, cellulose and lignin (R
2 = 0.72) than by lignocellulose in the ADF fraction. Our results indicated that proximate analyses were not sufficient to
explain differences in decomposition. To predict C mineralisation from the range of plant materials studied, we propose a
minimum set of analyses comprising total N, free sugars, starch, arabinose, xylan, cellulose and lignin. 相似文献
183.
An understanding of the effects of salinity and sodicity on soil carbon (C) stocks and fluxes is critical in environmental
management, as the areal extents of salinity and sodicity are predicted to increase. The effects of salinity and sodicity
on the soil microbial biomass (SMB) and soil respiration were assessed over 12weeks under controlled conditions by subjecting
disturbed soil samples from a vegetated soil profile to leaching with one of six salt solutions; a combination of low-salinity
(0.5dSm−1), mid-salinity (10dSm−1), or high-salinity (30dSm−1), with either low-sodicity (sodium adsorption ratio, SAR, 1), or high-sodicity (SAR 30) to give six treatments: control (low-salinity
low-sodicity); low-salinity high-sodicity; mid-salinity low-sodicity; mid-salinity high-sodicity; high-salinity low-sodicity;
and high-salinity high-sodicity. Soil respiration rate was highest (56–80mg CO2-C kg−1 soil) in the low-salinity treatments and lowest (1–5mg CO2-C kg−1 soil) in the mid-salinity treatments, while the SMB was highest in the high-salinity treatments (459–565mg kg−1 soil) and lowest in the low-salinity treatments (158–172mg kg−1 soil). This was attributed to increased substrate availability with high salt concentrations through either increased dispersion
of soil aggregates or dissolution or hydrolysis of soil organic matter, which may offset some of the stresses placed on the
microbial population from high salt concentrations. The apparent disparity in trends in respiration and the SMB may be due
to an induced shift in the microbial population, from one dominated by more active microorganisms to one dominated by less
active microorganisms. 相似文献
184.
Mario Augusto Pagnotta Linda Mondini Pasquale Codianni Clara Fares 《Genetic Resources and Crop Evolution》2009,56(3):299-310
Emmer wheat (Triticum dicoccon Schrank, 2n = 4x = 28) consists in a hulled wheat; its cultivation has been drastically reduced during the last century as a consequence of
its low yield. Recently, its agronomic and nutritive values, as well as the increase of popularity of organic agriculture,
have led to a renewed interest making its cultivation economically viable in the marginal lands with an increase of the cultivated
areas. In Italy, it mainly survives in few marginal lands of central and southern Italy, where local varieties, adapted to
the natural environment from where they originate, are used; moreover, some selected lines have also been developed. In the
present work, agro-morphological and qualitative traits, together with molecular analyses of 20 emmer accessions consisting
of Italian landraces, breeding lines, and cultivars, were performed. The field experiments were conducted for two consecutive
years (2001/2002–2002/2003) in two locations: Viterbo in central Italy, and Foggia in south Italy. The analyzed emmer wheat
accessions showed a good amount of genetic variability for both evaluated agro-morphological and molecular traits. This study
illustrates an increase in earliness, GY, TW, TKW, and YI going from landraces, breeding lines to cultivars, while the variability
does not change proportionally. 相似文献
185.
Nahla Bassil Kim E. Hummer Joseph D. Postman Gennaro Fazio Angela Baldo Isabel Armas Roger Williams 《Genetic Resources and Crop Evolution》2009,56(3):339-352
Heritage apple (Malus domestica Borkh. hybrids) and pear (Pyrus communis L. hybrid) trees grow in villages throughout Terceira Island, Azores, Portugal. Some of these pears have different names but similar morphology. The objective of this study was to determine synonymy, homology, and phylogeny of apples and pears from Terceira and to examine potential relationships of the island pears with standard apples and pears of Portuguese or American descent. Nine apple microsatellite markers were used to determine genetic relationships. Distance- and parsimony-based cluster analysis grouped these genotypes into separate apple and pear clades. The Terceira apples were divided into two clades: the maçā and the reineta-reinette. Among the 17 heritage apple genotypes, seven unique accessions were identified and four groups of synonyms, or possibly clones, were detected including: ‘Reineta Agosto’ and ‘Reineta Verde’ from Altares; ‘Reineta Castanha’ and ‘Reineta Verde Miuda’; ‘Maçā Pêra,’ ‘Maçā Calhau’, ‘Pêro Branco’ from Salga and from Terra-Chā and ‘Maçā Marmelo’; and the five genotypes ‘Maçā Sao Joao’, ‘Malápio Rosa’, ‘Maçā Gaspar’, ‘Maçā Branca’ and ‘Maçā Pato’. In addition, two homonyms were detected. ‘Pêro Vermelho’ from Terra Chā was a separate genotype from a tree from Doze Ribeiras of the same name, but Pêro Branco from Terra Chā appears to be a clone that can be distinguished by an additional allele at CH1F07a from a tree with that name from Salga. One pair of apple clones, ‘Reineta Agosto’ and ‘Reineta Verde’ from Altares appear to be derived from an unreduced gamete of ‘Golden Delicious.’ Another apple genotype ‘Maçā Acida’ could be a sibling of the ‘Maçā Pêra’ clonal group. Other tested standard apples from the US genebank were unrelated to Terceira genotypes. Of the seven heritage pears, five unique genotypes and one pair of synonyms were detected. ‘Pêra Papo Pintassilgo’ from Raminho and ‘Pêra Vermelha’ from the nursery of Serviço de Desenvolvimento Agario da Terceira (SDAT) were synonyms. ‘Passans du Portugal’ was related to ‘Pêra Cabaca’ but other standard pears from the US genebank were unrelated to Terceira genotypes. Future studies will include additional apple and pear cultivars from other Islands of the Azores and continental Portugal, and wild Asian species to further explore genetic relationships. 相似文献
186.
187.
PCB removal,soil enzyme activities,and microbial community structures during the phytoremediation by alfalfa in field soils 总被引:1,自引:1,他引:1
Chen Tu Ying Teng Yongming Luo Xianghui Sun Shaopo Deng Zhengao Li Wuxing Liu Zhihong Xu 《Journal of Soils and Sediments》2011,11(4):649-656
Purpose
An in situ phytoremediation trial was developed in order to investigate the function of alfalfa during a 2-year bioremediation of an agricultural soil contaminated with polychlorinated biphenyls (PCBs). The study was conducted with the aim to better understand the application potential of PCB phytoremediation at field scale. 相似文献188.
This study examined the effects of contact with wood on the living human body using a physiological index and subjective evaluation.
Consecutive blood pressure measurements were used as the physiological index, and sensory evaluation using the semantic differential
(SD) method was used for subjective evaluation. Consideration was also given to cases in which materials were cooled and heated
as well as kept at room temperature, to eliminate the effects of heat flux due to differences in thermal conductivity between
wood and other materials. It was found that contact with wood produced coarse/natural sensations, with no associated increase
in systolic blood pressure. Contact with cold wood created subjectively dangerous/uncomfortable but still coarse/natural sensations,
also with no associated increase in blood pressure; therefore, there was no correspondence between subjective evaluation and
physiological responses. Contact with aluminum kept at room temperature and cold acrylic plastic created flat/artificial and
dangerous/uncomfortable sensations, with an associated significant increase in blood pressure; thus, there was a close correlation
between subjective evaluation and physiological responses. It was therefore concluded that contact with wood, unlike artificial
materials such as aluminum, induces no physiological stress even when kept at room temperature or cooled.
Part of this report was presented at the 48th Annual Meeting of the Japan Wood Research Society in Shizuoka, April 1998 相似文献
189.
Ye Tian Kikuo Haibara Hiroto Toda Fangjun Ding Yanhui Liu Dongsu Choi 《Journal of Forest Research》2008,13(4):205-214
Mountain closure, considered an effective and economic measure for natural restoration of degraded forest ecosystems, has
been widely carried out in the karst region of southwest China. The aim of this study was to evaluate microbial aspects of
soil quality after mountain closure by analyzing soil microbial biomass, basal respiration, metabolic quotient, and relationships
with basic chemical properties in Guizhou Province, a karst region of the upper Yangtze River. Soil quality was considered
poor from the low levels of microbial biomass carbon (MBC), nitrogen (MBN), and microbial quotient (MBC/total C and MBN/total
N), but high metabolic quotient (qCO2). Soil pH, showing marked variation from 4.1 to 7.9 in this karst region, was proved to significantly affect soil microbial
biomass and activity. Soil microbial biomass, microbial quotient, and soil basal respiration declined significantly with decreasing
soil pH, while qCO2 showed an apparently increasing, but not statistically significant, trend. The changes in microbial biomass and activity
following the change in soil pH could possibly be because of a change in soil microbial composition, and more detailed research
is necessary. Compared with soil pH, soil organic matter content was another, more important, factor that directly restricted
microbial growth because of the serious loss as a result of disturbance. As a practical application based on microbial aspects,
introduction of some N-fixing tree species may be an active and effective measure to improve soil fertility and thus to accelerate
restoration of the forest ecosystem in the karst region. 相似文献
190.
In Japan, ammoniacal copper quaternary wood preservatives type-1 (ACQ-1), which contains copper and benzalkonium chloride
as its active ingredients, is among the most widely used wood preservatives in the pressure treatment of wood. Benzalkonium
chloride (BAC) in commercial ACQ-1 products mainly comprises C12 and C14 homologues. In the present study, the leaching characteristics
of these BAC homologues were investigated using the heartwood and sapwood portions of Japanese cedar, Japanese larch, and
Sakhalin fir treated with ACQ-1 and 1% monoethanolamine (MEA) solution containing equimolar amounts of homologues. Distilled
water (DW) and artificial seawater (SW) were used as leaching media. Consequently, it was observed that the leaching rate
of the C12 homologue tended to be higher than that of the C14 homologue in DW. The leaching of C12 homologues was accelerated
by using SW, resulting in a significantly higher leaching rate than the C14 homologue using SW. It was thought that the difference
in the hydrophobicities based on alkyl chain lengths resulted in these phenomena. However, when the heartwood portion of Japanese
larch was treated with the homologues in MEA, the leaching rate of the C14 homologue was significantly higher than that of
the C12 homologue. 相似文献