Microsatellite mapping of genes for semi-dwarfism and branched spike in Triticum durum Desf. var. ramosoobscurum Jakubz. “Vetvistokoloskaya”

A combination of increased harvest index and increased seed number per plant may improve wheat yield. We found an accession Triticum durum Desf. var. ramosoobscurum Jakubz. “Vetvistokoloskaya” R-107, whose plant height was short and spike was branched. To characterize R-107, we mapped the genes for semi-dwarfism and branched spike. Semi-dwarf gene Rht_R107 (Reduced height R107) from R-107 was allelic to Rht11 and Rht-B1b on chromosome 4BS. Rht_R107, as well as Rht11, was recessive and insensitive to gibberellic acid. Microsatellite mapping indicated that Rht_R107 was linked to Xwmc48 (1.2?cM) on chromosome 4BS, and Rht11 was also linked to Xgwm495 marker (19.4?cM) on chromosome 4BS. The locus responsible for branched spike in R-107 was mapped by genotyping three F₂ populations using microsatellite markers. Phenotypic analysis revealed that branched spike was under control of a recessive allele at a single locus. The bh (branched head) locus was located on chromosome 2AS and the marker Xgwm425 flanked the bh gene proximally.     

Effects of shifting cultivation on biological and biochemical characteristics of soil microorganisms in Khagrachari hill district,Bangladesh

We collected soil samples from two representative sites at Aatmile of Khagarachari hill district in Chittagong Hill Tracts. One of the sites was under shifting cultivation and the other an adjacent 13-year old teak plantation. Both sites were in the same physiographic condition and same aspect with parable soil type, which enabled us to measure the effects of shifting cultivation on soil micro-flora. We studied soil physico-chemical properties and the biochemical and biological properties of soil microbes. Moisture and organic matter content as well as fungi and bacterial populations, both in surface and subsurface soils, were significantly(p ≤0.001) lower in shifting cultivated soils compared to soils not under shifting cultivation, i.e. the teak plantation site. The most abundant bacteria in surface(0-10 cm) and sub-surface(10-20 cm) soils under shifting cultivation were Pseudomonas diminuta and Shigella, respectively, while in corresponding soil layers of teak plantation, predominant microbes were Bacillus firmus(0-10 cm) and Xanthomonas(10-20 cm). The microbial population differences cannot be explained by soil texture differences because of the textural similarity in soils from the two sites but could be related to the significantly lower moisture and organic matter contents in soils under shifting cultivation.     

Weeds

Abstract

The effects of simulated rat damage (stem cutting) on IR-8 rice yield was examined. Fields were subjected to four damage levels: 0 (control), 10, 25 and 50% of the stems cut. A modified split-plot sampling design was used with ten 1 m² plots tested at each damage level in three growth stages: tillering, booting and maturity. Each of the 120 plots (2,400 hills) was harvested and yields compared by Analysis of Variance and Least Significant Difference (LSD) tests. Ten perpent of all stems removed during the tillering stage produced growth compensation and a higher yield resulted. Trends in rice yields for different damage levels showed that the later damage occurred, the greater the yield loss. An LSD analysis of yields for the damage levels revealed no significant differences during tillering. At booting, significant differences (P < 0.05) in yields were noted with > 10% of the stems cut. At maturity, yields for all damaged levels differed significantly (P < 0.01). The results of this study demonstrated that rat damage in rice up to the booting stage did not affect yield significantly. From an economic standpoint, rodent control by field baiting before booting stage is not recommended in monsoon rice.     

Apical Meristem Tip Culture for Eradication of Flexous Rod Viruses in Yams (Dioscorea alata)

Abstract

Apical meristem tips excised from vines of Dioscorea alata cv. White Lisbon produced normal plantlets when cultured in vitro on Murashige and Skoog basal medium supplemented with a range of auxins and cytokinins. Highest yields were obtained on basal medium supplemented with either 1.0 or 0.5 mg/l 2-naphthaleneacetic acid, in combination with either 0.2 or 0.1 mg/l 6-benzyladenine, respectively. Eradication of flexous rod virus infections was possible only when meristems were excised from mother plants which had been growing at 36 + 1°C for at least 14 days; progeny of plantlets obtained from such meristems remained free of detectable virus infection as judged from symptomless growth for 16 months and electron microscopic examination of both sap and ultrathin sections of leaf tissue.     

Crossbred bull selection for bigger scrotum and shorter age at puberty with potentials for better quality semen.

Shortening age at puberty of crossbred breeding bull is an important issue in the tropics. This study aimed at selecting crossbred bulls at earliest possible age with bigger scrotum and potential for donating quality semen. One hundred and 31 pre-joining crossbred bulls of Central Artificial Insemination Laboratory, Saver, Dhaka were examined. The bulls being trained by seeing semen collection from mature bulls were allowed ejaculation into the artificial vagina at homosexual mount during a 20 min time at three occasions, every three months. Eighty one of 131 bulls produced at least one ejaculate during the study and their mean +/- SD age and scrotal circumference (SC) were 20.3 +/- 4.7 months and 28.2 +/-2.7 cm, respectively. Bulls' body weight, body condition score (BCS) and SC influenced the attainment of their puberty (p < 0.05). Bull's body weight had positive effects on scrotal circumference and ejaculate volume (p < 0.05). Scrotal circumference positively influenced the percentages of normal spermatozoa (p < 0.05). Scrotal skin-fold thickness negatively influenced the proportion of spermatozoa with normal head (p < 0.05). Based on age at first ejaculate and SC, 29.6% bulls (n = 24) were selected by cluster analysis. Selected bulls had mean +/- SD age 17.9 +/- 2.2 months, body weight 287.3 +/-48.6 kg, SC 30.5 +/- 1.5 cm, ejaculate volume 3.4 +/- 1.3 ml, sperm motility 50.8 +/- 17.2%, total spermatozoa per ejaculate 2541.9 +/- 1699.2 million and normal spermatozoa 81.5 +/-14.5%. The selected pubertal bull group was different from the unselected pubertal bulls at MANOVA (p < 0.0001). About 30% of pubertal crossbred bulls can be selected with shorter age and larger scrotum at puberty under conditions prevailed in Bangladesh.     

An improved micropropagation protocol for the recalcitrant plant Capsicum – a study with ten cultivars of Capsicum spp. (C. annuum,C. chinense,and C. frutescens) collected from diverse geographical regions of India and Mexico

Capsicum spp. is a commercially important crop of the Solanaceae family, well-known for its multipurpose use as a vegetable, spice, medicinal and ornamental plants. The genus Capsicum is a recalcitrant species in terms of in vitro morphogenesis and plant regeneration. An efficient method was developed for multiple shoot regeneration in 10 cultivars of Capsicum collected from diverse geographical regions of India and Mexico. Seeds germinated in vitro on a half-strength Murashige and Skoog (MS) medium supplemented with 3.0 % sucrose. Nodes of the in vitro germinated seedlings were used as explant for micropropagation. The combination of the 6-benzylaminopurine, indole-3-acetic acid, and spermidine was found to be the best for multiple shoot induction. However, the optimum responcse varied accompanied by different cultivers with maximum 8.9 ± 0.52 (Capsi-10) to 15.3 ± 0.69 (Capsi-5) multiple shoot per explant. Depending on the cultivar, multiplied shoots were successfully rooted with maximum 18.4 ± 0.20 (highest for Capsi-9) to 36.8 ± 0.29 (highest for Capsi-5) roots per shoot on half-strength MS medium supplemented with 2.0 mg l^?1 indole-3-butyric acid, 1.0 mg l^?1 α-naphthalene acetic acid, and 1.5 mM spermidine. Finally, the micropropagated plantlets were acclimatized with 40.0–86.7 % survival rate, depending on different cultivars.     

Pathogenicity of Phytophthora alni complex and P. plurivora in Alnus glutinosa seedlings

The ecological importance of riparian forests is well known. However, these forest habitats have been disturbed by human activities over the past century as a result of the introduction of flow regulations. Mortality of the riparian alder population caused by Phytophthora has become an important issue in Europe in the past two decades. The main objective of this study was to examine the pathogenicity of the Phytophthora alni complex (P. xalni, P. uniformis and P. xmultiformis) and P. plurivora in Alnus glutinosa seedlings. Phytophthora alni complex has traditionally been identified as the main causal agent of alder decline; however, in this study, P. plurivora was found to cause as much damage in inoculated seedlings as P. alni complex. In fact, Spanish isolates of P. xalni caused mortality rates of ca. 30%, whereas P. plurivora killed ca. 50% of seedlings. Likewise, there were no differences between P. xalni ‐ and P. plurivora‐inoculated seedlings in either the length of lesion or biomass allocation. The pathogenicity of the species within P. alni complex did not differ significantly. The findings confirm that P. plurivora, which is one of the most common species of Phytophthora found in European nurseries, is highly pathogenic to alder seedlings. Urgent implementation of measures to prevent the risk of spread of the pathogen from nurseries to natural ecosystems is required. Further studies are required to clarify the role that P. plurivora is playing in alder decline both individually and in combination with P. alni complex.     

Leucaena hedgerow intercropping and cattle manure application in the Ethiopian highlands III. Nutrient balances

 Balances between nutrients applied or mineralized and nutrients removed in maize grain and stover were calculated in a hedgerow intercropping experiment in which Leucaena leucocephala and L. pallida prunings and cattle manure were applied. Hedgerow intercropping (also called alley cropping) is an agroforestry system in which trees are grown in dense hedges between alleys where short-cycle crops are grown. The hedges are pruned periodically during the cropping period and the prunings are added to the soil as green manure. In control treatments, nutrient depletion per season was in the order of 7–19 kg N ha^–1, 4–12 kg P ha^–1, 10–26 kg K ha^–1, 0–2 kg Ca ha^–1 and 3–6 kg Mg ha^–1. N fertilizer reversed the depletion of N, but it accelerated the depletion of the other nutrients. Manure and at least two applications of leucaena prunings resulted in net positive balances of N, K, and Ca between amounts applied or mineralized and amounts removed by maize. The amounts of P and Mg applied with, or mineralized from, prunings or manure were insufficient to offset the negative balances of these nutrients. Received: 27 January 1998     

The influence of different hormone concentration and combination on callus induction and regeneration of Rauwolfia serpentina L. Benth

The influence of media composition on callus induction and subsequent regeneration of Rauwolfia serpentina L. Benth has been studied. High frequency (96.43%) callus induction was obtained when nodal segments from in vitro raised shoots were cultured on MS medium supplemented with 0.5 mg L(-1) BA and 2.0 mg L(-1) NAA. The callus differentiated into adventitious shoots when it was subcultured on MS medium supplemented with 2.0 mg L(-1) BA with 0.2 mg L(-1) NAA. Regenerated shoots were best rooted on half-strength MS medium with 1.0 mg L(-1) each of IBA and IAA.     

Evaluation of a Near‐Infrared Reflectance Spectrometer as a Granulation Sensor for First‐Break Ground Wheat: Studies with Hard Red Winter Wheats

A single wheat class or blended wheats from two wheat classes are usually milled in a flour mill. A near‐infrared (NIR) reflectance spectrometer, previously evaluated as granulation sensor for first‐break ground wheat from six wheat classes, was evaluated for a single wheat class, hard red winter (HRW) wheat, using offline methods. The HRW wheats represented seven cultivars ground by an experimental roller mill at five roll gap settings (0.38, 0.51, 0.63, 0.75, and 0.88 mm) which yielded 35 ground wheat samples each for the calibration and validation sets. Granulation models based on partial least squares regression were developed with cumulative mass of size fractions as a reference value. Combinations of four data pretreatments (log 1/R, baseline correction, unit area normalization, and derivatives) and subregions of the 400–1,700 nm wavelength range were evaluated. Models that used pathlength correction (unit area normalization) predicted well each of the four size fractions of first‐break ground wheat. The best model, unit area normalization and first derivative, predicted all the validation spectra with standard errors of performance of 3.80, 1.29, 0.43, and 0.68 for the >1041, >375, >240, and >136 μm size fractions, respectively. Ground HRW wheats have narrower particle size distribution and better sieving properties than ground wheat from six wheat classes. Thus, HRW wheat granulation models performed better than the previously reported models for six wheat classes.