Effects of irrigation volumes and organic fertilizers on eggplant grown in Mediterranean environment

A three-year field experiment was carried out in Mediterranean conditions to study the effects of different irrigations and organic fertilizers on yield, quality and nitrogen utilization of eggplant crop and on soil properties. In a split-plot design, two irrigation treatments (re-establishing 100% and 50% of the calculated maximum evapotranspiration) and four fertilizer treatments (mineral fertilizer, commercial stable manure, anaerobic digestate and municipal solid waste compost) were compared. The highest water volume increased significantly the total and marketable yield, the number of marketable fruits, the dry matter of fruits and the nitrogen-use efficiency with respect to the treatment with the lowest irrigation. Nevertheless, the same treatment decreased significantly the fruit dry matter percentage and total soluble solid content. The lowest water volume led to an improvement of fruit quality. Anaerobic digestate increased significantly the total and marketable yield and dry matter compared with compost. Harvest index, nitrogen harvest index, pH, acidity and total soluble solids did not show significant differences among all fertilizer treatments. N translocation ability of plants and fruit quality were not influenced by the type of fertilizer. At the end of experiment, anaerobic digestate and compost reached the highest levels of soil total organic carbon and humic and fulvic acids content. Besides, anaerobic digestate increased the total extractable carbon, phosphorus and nitrogen content compared to all other treatments. Soil copper, nickel and lead content in all treatments enhanced compared to that at the beginning of experiment, but this accumulation did not represent a possible environmental risk. The content of heavy metals in the soil, after 3 years of application, did not exceed the limits imposed by the Italian law. This study showed that the organic wastes, after being adequately processed, could be applied successfully in agriculture especially in arid environments, characterized by quick mineralization and very low organic matter content.     

YIELD AND SOIL RESPONSES OF MELON GROWN WITH DIFFERENT ORGANIC FERTILIZERS

A three-year field experiment (2006–2008) was carried out in Mediterranean conditions to study the effects of organic fertilizers application on yield performance and plant nitrogen (N) status of melon crops. Soil properties at the end of experiment were further investigated. In a strip-plot experimental design, two irrigations and the following four fertilizer treatments were compared: mineral fertilizer (Min); commercial stable manure (Org); anaerobic digestate (WDD); composted municipal solid organic wastes (SUW). The results showed no statistical differences among the four different treatments on melon yields. Conversely, the highest irrigation treatment showed a significantly higher total yield (34%) and fruits number (26%). During cropping cycles, the Min treatment reached the highest mean nitrate content with an increase of about 59% and 154% respectively compared with Org and mean of WDD and SUW. The WDD and SUW significantly increased soil total, extracted and humified organic carbon of the 14, 22 and 12% compared with Min. Among treatments, no significant difference was found for heavy metals excepted for Cu, which reached the highest level in WDD treatment.     

From the lab to the river: Determination of ecological hosts of Anodonta anatina

1. Coextinction is the simplest form of secondary extinction and freshwater mussels (Bivalvia, Unionida) may be particularly prone to this phenomenon as their life cycle includes an obligatory parasitic larval stage on fish hosts.
2. The main aims of this study were to determine the possible ecological fish hosts of Anodonta anatina (Linnaeus, 1758) in several rivers of the Douro basin in northern Portugal and to assess possible spatial and temporal differences in glochidial (larval) loads. In order to achieve this, electrofishing was conducted from December to April, the fish fauna was characterized, and levels of infestation with A. anatina glochidia were determined.
3. Native cyprinid species, mainly Luciobarbus bocagei (Iberian barbel) and Squalius carolitertii (northern Iberian chub), together with the non‐native Lepomis gibbosus (pumpkinseed sunfish) and Alburnus alburnus (common bleak), were found to have the highest glochidial loads. Clear differences in infestation between rivers and throughout time were detected, with an infestation period from January to March, and with the Tâmega River having the highest prevalence.
4. Anodonta anatina is able to infest a variety of fish species, and this together with earlier studies showed that the metamorphosis into juveniles occurs mainly in native cyprinid species, although non‐native species like common bleak can also be considered suitable hosts. However, the larvae infesting other non‐native species, such as the pumpkinseed sunfish, do not metamorphose and can be considered ‘dead ends’.
5. Overall, the results reported here are important for the conservation of A. anatina (and other unionoid species) because several Iberian rivers (and worldwide) have been subjected to the extirpation of native fish species and the introduction of non‐native fish species. Therefore, careful assessments of fish communities should be conducted to assess possible negative interactions with freshwater mussels.

     

A review of habitat thresholds for dead wood: a baseline for management recommendations in European forests

In contemporary forest management, also of commercial forests, threshold values are widely used for consideration of biodiversity conservation. Here, we present various aspects of dead-wood threshold values. We review published and unpublished dead-wood threshold data from European lowland beech–oak, mixed-montane, and boreo-alpine spruce–pine forests separately to provide managers of European forests with a baseline for management decisions for their specific forest type. Our review of dead-wood threshold data from European forests revealed 36 critical values with ranges of 10–80 m³ ha⁻¹ for boreal and lowland forests and 10–150 m³ ha⁻¹ for mixed-montane forests, with peak values at 20–30 m³ ha⁻¹ for boreal coniferous forests, 30–40 m³ ha⁻¹ for mixed-montane forests, and 30–50 m³ ha⁻¹ for lowland oak–beech forests. We then expand the focus of dead-wood threshold analyses to community composition. We exemplify the two major statistical methods applied in ecological threshold analysis to stimulate forest researchers to analyze more of their own data with a focus on thresholds. Finally, we discuss further directions of dead-wood threshold analysis. We anticipate that further investigations of threshold values will provide a more comprehensive picture of critical ranges for dead wood, which is urgently needed for an ecological and sustainable forestry.     

Prevalence of Leptospira interrogans antibodies in free-ranging Tayassu pecari of the Southern Pantanal, Brazil, an ecosystem where wildlife and cattle interact

We surveyed a wild population of white-lipped peccaries (Tayassu pecari) in the Brazilian Pantanal for evidence of Leptospira interrogans. Serum samples from 71 free-ranging T. pecari were obtained between 2003 and 2005 in the southern Pantanal of Mato Grosso do Sul state. We used microscopic microagglutination to test for antibodies against 14 L. interrogans serovars (antibody titers ≥1:100 were considered seropositive). Seventy percent of captured animals tested positive for leptospirosis antibodies. Antibodies against icterohaemorrhagiae and autumnalis serovars were the most prevalent. We used log-linear analyses to test for associations among seropositivity, age class, and sex of captured animals. Seropositivity was strongly associated with animal age class, but independent of sex. Forty-six percent of animals less than 2 years old, 63% of adults during peak reproductive years, and 100% of the oldest age class were seropositive. A nonparametric multivariate procedure (MRPP) showed that the composition of serovar antibody types changed with age, and ANOVA models demonstrated that antibody titers increased with age, suggesting long-term exposure to a greater number and variety (i.e., serovar types) of L. interrogans infections. This study presents the first quantitative survey of antibodies against L. interrogans serovars in a T. pecari population of the Pantanal. The high prevalence of leptospirosis antibodies in free-ranging white-lipped peccaries and the potential impacts on reproduction and population dynamics emphasize the need for further studies investigating the roles of Pantanal wildlife and livestock in the transmission and maintenance of L. interrogans in the environment.     

Dynamics of scale regeneration in seawater- and brackish water-acclimated sea bass, Dicentrarchus labrax

Scale loss is a common occurrence in both wild fish and those in aquaculture production systems, and regeneration of scales has been described in several freshwater species. Relatively little information exists about this process in marine fish, and in the present study, the chronology of scale regeneration was characterized in juvenile sea bass Dicentrarchus labrax, maintained in full seawater (SW; 36 ‰, 11.2 mM Ca²⁺) or brackish water (BW; 3.5 ‰, 1.1 mM Ca²⁺). Despite the significant differences in plasma osmolality, plasma calcium (Ca) and phosphorus (P) were similar between SW and BW. Removal of scales (2.25 cm²) did not modify plasma osmolality but a significant reduction in plasma Ca occurred in sea bass in BW during early phases of scale regeneration. Scale Ca and P contents changed during regeneration in SW and BW fish, and in fully regenerated scales, their concentration was comparable to ontogenic scales which had a Ca/P ratio around 1.0. Regenerating scales were first visible at 3 days after removal, and by 6 days, the descaled area was covered with new scales in both SW and BW fish. From this stage onwards, while scale area and mass rose gradually, a rapid accretion of calcium crystals was observed over the basal plate, especially in SW, and Ca and P content per scale increased at a very high rate, decreasing only at the later stages, and between 21 and 30 days the newly formed scales had already ontogenic-like morphometric features. Scale tartrate-resistant acid phosphatase (TRAP) or alkaline phosphatase (ALP) activity was initially similar in SW and BW. However, during regeneration, TRAP was significantly higher in scales of sea bass in SW compared to those in BW where ALP predominated; these differences persisted at 30 days.