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2. Hepatocytes that stained with trypan blue were considered to be dead or dying before fixation and represented damaged cells.
3. The proportion of trypan blue‐stained hepatocytes in the livers of ascitic birds was slightly less than half that observed in the hypoxia‐induced birds but significandy more than the proportion of stained cells observed in control birds.
4. Liver damage in the ascitic birds was also assessed biochemically by an altered enzyme profile.
5. The study demonstrated that increased trypan blue uptake in the livers of ascitic birds reared at sea‐level may be the consequence of hypoxia stress caused by reduced oxygen utilisation. 相似文献
2. In 3 treatments the diet was supplemented with 0, 100 and 150 mg probiotic/kg food.
3. In the 100 mg probiotic group, egg production improved by 5%, and shell thickness improved slightly, with fewer thin‐shelled eggs than in the control (8.6% compared to 18.6%).
4. The initial serum cholesterol concentration of 170.2 mg/dl in control birds remained similar throughout the 10‐week experimental period, whereas in the 150 mg group the initial value of 176.5 mg/dl decreased to 114.3 mg by week 10.
5. Yolk cholesterol concentration was 14.69 mg in the control group and 11.28 and 11.37 mg/g in the 100 and 150 mg probiotic groups respectively. Overall mean total egg cholesterol was thus reduced by probiotic supplementation. 相似文献
2. Daytime perching did not differ significantly between the treatments. At night, over the whole year, 81% of birds in the 480 mm cages and 86% in the 520 mm cages roosted on the perch. This figure reached about 95% in the 560 and 600 mm cages, significantly more at most ages than in the 480 mm cages.
3. Feather damage was slightly less, but claw problems slightly more, in the 2 wider treatments than in the 2 narrower treatments. Birds in the wider cages were calmer when approached or handled by humans than those in the narrower cages. This may have been associated with variation in space allowance between the treatments.
4. There was a trend for lower production in the 480 mm cages than in the other treatments which may have been associated with the reduced feeding space in this treatment. There were few other treatment differences in production traits.
5. The balance of the evidence from this study is that when perches are provided in laying cages for medium weight hybrids, 140 mm of perch space per hen is adequate. For the amelioration of a number of the welfare problems of conventional cages, provision of perches should be combined with other modifications. 相似文献
2. Birds in all treatments gained similar amounts of weight from 12–14 weeks of age. Thereafter, birds in C maintained gains, the body weight gains of birds in EC and EH declined moderately and birds in H lost weight.
3. Birds in C produced more metabolic heat. Those in H showed increased heat production as they became older.
4. Protein retention was greater in turkeys in C, EC and EH. It was lower in H, becoming negative between 18–20 weeks of age. Birds in H suffered body fat losses which increased with age.
5. Birds in EH and EC gained similar amounts of body weight. Birds in EH exhibited lower heat production and greater energy retention than those in EC.
6. Body temperatures were higher in H than in the other environments. 相似文献
2. Culture media deficient in either sodium or chloride ions reduced the volume of SEF secreted by up to a quarter. Ionic composition of the fluid was little altered, and sodium was transported against a concentration gradient.
3. Amiloride, an inhibitor of Na+/H+ exchange, reduced SEF production by half, whereas ionic composition and osmolality of the fluid was unchanged.
4. Likewise, acetazolamide, an inhibitor of carbonic anhydrase, reduced SEF production by the blastoderm, and left the ionic composition and osmolality of the fluid unaltered.
5. Neither furosemide or 4,4´‐diisothiocyanatostilbene‐2,2´‐disulfonic acid at the concentrations used changed the volume of SEF formed or its ionic composition.
6. It is concluded that the secretion of SEF is dependent upon the active transport of sodium across the blastoderm; an amiloride‐sensitive Na+/H+ exchanger and carbon dioxide hydration catalysed by carbonic anhydrase are also involved.
7. Furthermore, it is proposed that fluid transport across the blastoderm is the result of local osmotic gradients, not from a sub‐embryonic fluid hyperosmotic to albumen, as has been suggested previously. 相似文献