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41.
Low level of polymorphism detected by SSR probes in bread wheat 总被引:2,自引:1,他引:1
R. K. Varshney P. C. Sharma P. K. Gupta H. S. Balyan B. Ramesh J. K. Roy A. Kumar A. Sen 《Plant Breeding》1998,117(2):182-184
In-gel hybridization patterns were studied in a set of nine diverse bread wheat ( Triticum aestivum L. em. Thell) genotypes using 23 simple sequence repeat (SSR) probes in combination with 14 different restriction enzymes. Multilocus fingerprints due to SSR probes, shown earlier to be characteristic of a majority of plant genomes, were not obtained and only a very low level of polymorphism was detected when using as many as 142 probe-enzyme combinations. The hybridization of a prominent solitary high molecular weight fragment (> 23 kb) with a number of SSR probes suggested the presence of these SSRs (microsatellites) within the long stretches of repeated DNA sequences. This indicates that the genome of bread wheat differs from that of other plants in the organization and distribution of SSRs and that SSR probes detect very little polymorphism. 相似文献
42.
An Erratum for this article has been published in Pest Management Science 56(5) 493 (2000). The degradation of the insecticide lindane (γ‐hexachlorocyclohexane, γ‐HCH) by two white‐rot fungi, Cyathus bulleri and Phanerochaete sordida, was studied. C bulleri degraded lindane more efficiently than P sordida. Two degradative intermediates identified in P sordida culture were tetrachlorocyclohexene and tetrachlorocyclohexanol. However, tetrachlorocyclohexanol was the sole degradation product detected in cultures of C bulleri. The presence of lindane only inside the mycelial cells of both fungi eliminated any role of intracellular enzymes during initial steps of its degradation. The insecticide at 0.27 µM showed no adverse effect on fungal growth. © 2000 Society of Chemical Industry 相似文献
43.
Bradley N Metz Priyadarshini Chakrabarti Ramesh R Sagili 《Journal of insect science (Online)》2021,21(6)
Honey bee larvae are dependent on the social structure of colony for their provisioning and survival. With thousands of larvae being managed collectively by groups of foragers (collecting food resources) and nurse bees (processing food and provisioning larvae), coordination of colony efforts in rearing brood depends on multiple dynamic cues of larval presence and needs. Much of these cues appear to be chemical, with larvae producing multiple pheromones, major being brood ester pheromone (BEP; nonvolatile blend of fatty acid esters) that elicits both short-term releaser effects and long-term primer effects. While BEP can affect colony food collection and processing with the signaling of larval presence, it is unclear if BEP signals individual larval needs. To understand this aspect, in a series of experiments we manipulated larval feeding environment by depriving larvae from adult bee contact for 4-h period and examined (1) nurse bee interactions with contact-deprived and nondeprived larvae and larval extracts; (2) forager bee responses to contact-deprived and nondeprived larval extracts. We also characterized BEP of contact-deprived and nondeprived larvae. We found that nurse honey bees tend to aggregate more over contact-deprived larvae when compared with nondeprived larvae, but that these effects were not found in response to whole hexane extracts. Our analytical results suggest that BEP components changed in both quantity and quality over short period of contact deprivation. These changes affected foraging behavior, but did not appear to directly affect nursing behavior, suggesting that different chemical cues are involved in regulating nursing effort to individual larvae. 相似文献
44.
Ramesh Chandra Malick Asit Kumar Bera Hemanta Chowdhury Manojit Bhattacharya Tanuja Abdulla Himanshu Sekhar Swain Raju Baitha Vikas Kumar Basanta Kumar Das 《Aquaculture Research》2020,51(6):2410-2420
The disease outbreaks in aquaculture system of wetlands are the major cause of fish mortality. Among various bacterial septicaemic diseases, fish mortality caused by Acinetobacter spp. is recently reported in different fish species. Fish disease outbreak was investigated in a wetland of West Bengal, India to identify the aetiological factors involved. The moribund fish were examined and subjected to bacterial isolation. Two bacterial causative agents were identified as Acinetobacter junii and Acinetobacter pittii by biochemical characterization and 16S rRNA gene amplification. Both the isolates were oxidase‐negative, nitrate‐negative, catalase‐positive and indole‐negative. The molecular identification using 16S rRNA gene sequencing and phylogenetic tree analysis further confirmed the two Acinetobacter spp. with 97%–99% similarity. The antibiotic resistance patterns of these two bacteria revealed that both of them were resistant to β‐lactam, cefalexin, cephalothin, amoxyclav, cefuroxime, cefadroxil, clindamycin, vancomycin and penicillin. In addition, A. pittii was also resistant to other antibiotics of cephams group such as ceftazidime and cefotaxime. In the challenge experiment, both A. junii and A. pittii were found to be pathogenic with LD50 of 1.24 × 105 and 1.88 × 107 cfu/fish respectively. Histopathological examination of gill, liver and kidney revealed prominent changes supporting bacterial septicaemia. The investigation reports for the first time on concurrent infection by A. junii and multidrug‐resistant (MDR)‐A. pittii as emerging fish pathogens to cause severe mortality in Labeo catla and Hypophthalmichthys molitrix in a freshwater wetland. 相似文献
45.
46.
K. Ramesh K. Sammi Reddy I. Rashmi A.K. Biswas 《Communications in Soil Science and Plant Analysis》2016,47(3):369-374
Analysis of differential pore-volume distribution (DPVD) patterns of a commercial Clinoptilolite has been conducted experimentally using an analyzer by measuring the N2 adsorption isotherm. The commercial material was fractionated by sieving through sequential sieves: <125 µ (Z8; fine), 125–250 µ (Z9; medium), and >250 µ (Z10; coarse). The DPVD of the fractions were calculated from the hysteresis loop according to the adsorption and desorption curves of Dollimore-Heal (DH) model. The adsorption cycle had produced dissimilar differential pore volume distribution patterns. For the 10-nm pore width, fine and medium fractions had a maximum pore volume up to 0.0016 cm3/g nm, while the coarse fraction had a little greater value up to 0.002 cm3/g nm. Moreover, the medium fraction had too many hills and valleys in the DPVD. However, the desorption cycle–based DH analysis did not show any marked variation in the DPVD pattern. 相似文献
47.
Lakshman K. BABU Harnarain PANDEY Ramesh C. PATRA Artabandhu SAHOO 《Animal Science Journal》2009,80(2):149-156
A 2 × 3 factorial design was used to study the impact of rearing systems, individual (I) versus group (G) and different levels of milk/skim milk feeding (F1, F2 and F3) on hemato‐biochemical profile, disease incidence and average daily gain of crossbred (Bos indicus × Bos taurus) calves. Six calves were taken in each group on the basis of their birth weight and housed in individual (2.20 × 1.16 m2/calf) or in group pens (2.20 × 1.03 m2/calf). After 3 days of colostrum feeding, calves were allocated to one of three different milk feeding schedules: milk fed up to 8 weeks of age (F1), milk up to 4 weeks followed by 50% replacement by skim milk up to 6 weeks and 100% thereafter (F2) and 100% replacement of milk with skim milk after 4 weeks (F3). Calf starter and cereal green fodders were fed ad libitum from the second week of age and continued for 14 weeks. Parameters on health and disease profiles of calves (disease incidence, duration of illness, response to treatment and recovery) and weekly live weight change were recorded. Calf scour predominated (52.8%), followed by joint ill (25.0%) and respiratory infections (19.4%). The disease incidence was greater (P < 0.01) in individually housed calves (94.4 vs. 55.9%). The management of navel ill required longer recovery (7.01 days) followed by joint ill (4.87 days) and respiratory infection (4.86 days). The average daily gain during 0–14 weeks of age was higher (P < 0.01) in group‐housed calves (433 ± 22 vs. 355 ± 31 g), while the effect of feeding was not significant. Blood samples collected at 4, 8 and 14 weeks of age showed some periodic higher concentrations (but within normal range) of plasma urea and total protein in group housed calves on F2 and F3 feeding schedules in response to high protein intake. Other parameters remained non‐significantly different. Thus, group‐housed calves can be reared successfully with comparatively better performance and less illness than individually housed ones under the present health care and housing management system. However, the system should not be used as a substitute for good management, and frequent observations of calves should be an integral part of any successful rearing program. 相似文献
48.
Goldthioglucose induces in mice a significant increase in body weight, glucose, insulin and lipid levels. Treatment with 250 mg/kg of methanol and ethyl acetate extracts of Zingiber officinale for 8 weeks produces significant reduction in body weight, glucose, insulin and lipid levels as compared to obese control mice. The reduction in elevated glucose along with elevated insulin levels indicates that the treatment with Z. officinale improves insulin sensitivity. 相似文献
49.
Meera Ramesh Elizabeth Thomovsky Paula Johnson 《Canadian journal of veterinary research》2021,85(4):241
Dogs with lower airway pathology that present in respiratory distress often receive oxygen therapy as the first line of treatment regardless of the underlying cause. Conventional “low-flow” systems deliver oxygen with a maximum flow rate of 15 L/minute. Traditionally, when an animal’s respiratory status does not improve with conventional oxygen therapy and treatments for underlying disease, options might be limited to either intubation and mechanical ventilation or humane euthanasia. High-flow oxygen therapy (HFOT) has been gaining popularity in veterinary medicine as an alternative route of oxygen supplementation for animals that require support beyond conventional therapy. High-flow oxygen therapy can supply a mixture of air and oxygen via a heated and humidified circuit. It is user friendly and can be used in an environment in which mechanical ventilation is unavailable.This review article is written for emergency doctors and general practitioners who lack access to mechanical ventilation. This article briefly reviews pertinent respiratory physiology, traditional oxygen supplementation techniques, the physiology of HFOT, and the limited evidence available in veterinary medicine regarding the use of HFOT, its applications, and limitations. Guidelines for the use of HFOT are suggested and HFOT is compared to conventional therapy. 相似文献
50.
Sherin George P. R. Suresh P. A. Wahid Ramesh B. Nair K. I. Punnoose 《Agroforestry Systems》2009,76(2):275-281
The active root distribution pattern of mature rubber (Hevea brasiliensis Muell. Arg.) up to a lateral distance of 250 cm from the tree and to a soil depth of 90 cm was studied in an oxisol by employing
32P soil injection technique in Kerala, the state which accounts for 83% of rubber cultivation in India. The trees were aged
18 years and grown at a spacing of 4.9 × 4.9 m. The extent of absorption of applied 32P by the tree from various placements was assessed by radio assay of leaf and latex serum. Latex serum registered higher counts
and variability was less compared to leaf indicating the suitability of latex serum as a potential source for radio assay
for 32P studies in rubber. The results revealed that rubber is a surface feeder with 55% of the root activity confining to the top
10 cm of soil layer. Root activity declined with increasing depths and the concentration of physiologically active roots at
90 cm depth was only 6%. A more or less uniform distribution of root activity was noticed with respect to lateral distance
indicating more extensive spread of lateral roots. Concentration of physiologically active roots in the surface layer suggests
the possibility for competition under intercropped situation in mature plantations. 相似文献