Thyroid hormone modulation of ovarian recrudescence of air-breathing catfish Clarias gariepinus

In the present study, thiourea-induced thyroid hormone depletion and thyroxine (T₄) ‘overdose’ were used as a strategy to understand the influence of thyroid hormones on ovarian recrudescence of juvenile (3-months-old), immature (8-months-old) and adult (1-year-old) air-breathing catfish, Clarias gariepinus. Thiourea-induced thyroid hormone depletion in juvenile catfish impaired ovarian development, but no significant effect was observed in immature catfish and during late stage of ovarian recrudescence of mature catfish. T₄ treatment in females undergoing late stages of ovarian recrudescence induced rapid oocyte growth by promoting its early entry into maturational phase as evident from the presence of more number of vitellogenic and post-vitellogenic follicles, decrease in aromatse immunoreactivity and reduced estradiol–17β levels. Hence, thyroid hormones have an important role to play during early stages of ovarian development and vitellogenesis of catfish and also indicating that thyroid has a stage dependent effect on ovary.     

迪庆黄牛红细胞免疫功能研究及其意义

应用红细胞Ｃ３ｂ受体花环和免疫复合物花环试验,证实迪庆黄牛红细胞表面存在补体Ｃ３ｂ受体,表明红细胞免疫系统的概念适用于这种动物。文中对迪庆黄工细胞Ｃ３ｂＲＲ和ＩＣＲ分别代表红细胞表面Ｃ３ｂＲ“空位”和“占位”状态的含义做出解释。     

威宁县肉牛产业发展现状及对策

威宁县素有南方“畜牧之乡”的美誉,作为贵州肉牛产业发展的重点区域,威宁县按照“三年打基础、五年创品牌、十年磨一剑”的工作思路,扎实推进肉牛产业发展,取得了明显成效。但仍存在养殖方式粗放、产销融合不够、利益联结不充分、良种繁育体系不健全等问题。笔者结合实际,深入剖析现阶段威宁县肉牛产业发展现状及存在的问题,并提出有关对策,旨在为下一步威宁县肉牛产业高质量发展提供参考。     

香蕉3种主要病毒的多重PCR检测

根据香蕉束顶病毒(Banana bunchy top virus,BBTV)、黄瓜花叶病毒(Cucumber mosaic virus,CMV)和香蕉线条病毒(Banana streak virus,BSV)3种病毒核酸保守序列设计相应特异性引物,通过体系优化,建立了可以同时检测香蕉束顶病毒、黄瓜花叶病毒、香蕉线条病毒的多重PCR体系。     

家蝇免疫血淋巴对几种动,植物病原菌的抑菌作用

针刺损伤诱导的家蝇三龄幼虫免疫血淋巴对几种动、植物病原菌具有抗菌活性。同时、未经诱导的家蝇幼虫血淋巴也具有抗菌活性，但外源诱导能衡旺增强抗菌物质的活性，家蝇的免疫血淋巴中的抗菌物质具有热稳定性和耐冷冻贮藏的特性。     

草鱼肝胆综合症并发细菌疾病病原分离鉴定及药敏试验

对2016年6月江西省鄱阳县高家岭一个养殖场养殖草鱼发生的暴发性疾病进行病原分离鉴定及药敏试验,旨在确定其病因,为今后生产中有效防控该疾病提供参考。利用细菌分离方法在无菌条件下从患肝胆综合症草鱼体内分离、纯化致病菌,通过形态学、生化鉴定和16SrDNA序列分析、gyrB基因序列分析进行菌株鉴定,用攻毒试验找出草鱼的半致死浓度,并采用滤纸片扩散法进行药敏试验。从患肝胆综合症草鱼体内分离、纯化到一株致病菌,经鉴定为维氏气单胞菌,命名为PYG1。攻毒试验表明该菌能感染草鱼,且半致死浓度为1.5×10 ⁴ CFU/g鱼体质量。药敏试验结果表明该菌只对氨基糖苷类、第二代和第三代头孢类以及喹诺酮类的氧氟沙星共9种药物表现出敏感或中度敏感,对所测的其他药物耐药,为强耐药菌株。维氏气单胞菌PYG1是引起此次草鱼发病的致病菌,可选用氨基糖苷类、头孢类及喹诺酮类的氧氟沙星等药物进行防治。     

蛟河晒红烟移栽期和覆盖方式对烟株干物质积累和烟叶经济性状的影响

为揭示不同移栽期和覆盖方式对晒红烟干物质积累动态特征和有效积温关系的影响,以吉林蛟河晒红烟品种漂河1号为材料,研究3个移栽期（5月24日、5月29日、6月4日）和2种覆盖方式（不覆盖、地膜覆盖）对晒红烟生育期天数、有效积温、干物质积累特征及烟叶经济性状的影响。结果表明,提前移栽期可延长晒红烟伸根期天数;提前移栽期和地膜覆盖可延长晒红烟成熟期天数,提高了晒红烟成熟期有效积温和采收前干物质积累量,干物质积累速率峰值出现积温和快增期开始积温、结束积温均提前,降低干物质平均积累速率,提高快增期干物质积累量;移栽期提前,各处理产量下降,产值先升后降,地膜覆盖处理的上等烟比例和均价逐渐增加。相关性分析表明,干物质平均积累速率和干物质积累速率峰值出现积温对晒红烟经济性状的形成至关重要。因此,蛟河晒红烟在5月24日-5月29日移栽并采用地膜覆盖栽培较为适宜。     

甘蓝型油菜正季与反季材料小孢子的培养

本文以甘蓝型油菜Westar的F1代为供试材料,通过对8个正季和同样的8个反季材料进行小孢子培养对比实验。结果表明,相同材料（基因型）正季能获得胚状体的反季一样能获得胚状体,但是相同材料的出胚数反季要比正季少50%,并且出胚时间要晚5～8d。正季与反季材料的成苗百分率相同,平均达到93%,加倍率基本一样,达到80%。由此可见,用反季节材料培养小孢子同样能获得成功,对特殊材料可以利用此法进一步加速育种进程。     

Simple multiresidue method for monitoring of trimethoprim and sulfonamide residues in buffalo meat by high-performance liquid chromatography

A simple, specific, and rapid analytical method for the determination of trimethoprim (TMP) and three sulfonamide (SA) antimicrobial drug residues in buffalo meat is developed and validated. This method is based on a solid-phase extraction technique followed by high-performance liquid chromatography (HPLC)-photodiode array (PDA) detection. Target compounds were extracted from the meat by acetonitrile and water, cleaned up on a Bond Elute C 18 cartridge column, and separated on a RP-C 18 column during HPLC analysis. Acetonitrile along with water appears to be an excellent extractant as recovery of the analytes at maximum residues levels (MRLs) in spiked sample was in the range of 75-108%, with coefficient of variations (CVs) ranging between 1.34 and 22%. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.031 and 0.062 microg/g, respectively, for all of the compounds. Intra- and interday assay precisions of the method at 0.125 microg/g concentrations for any drug ranged between 3 and 4%. The linearities of the TMP, sulfadimidine (SDM), sulfadoxine (SDO), and sulfamethoxazole (SMX) were 0.9989, 0.9999, 0.9998, and 0.9997, respectively. For robustness, the analytical method was applied to 122 buffalo meat samples obtained from export meat processing plants.