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41.
运用文献计量法统计1979—2010年发表的盆栽观叶植物研究论文,从资源介绍、栽培养护、景观配置及生态功能等4个方面对我国盆栽观叶植物研究现状进行分析,针对研究中存在的问题提出改进建议,并总结我国盆栽观叶植物研究未来的发展趋势。 相似文献
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ZHAO Xin RUAN Zi-yun GUO Zhen-wei ZHOU Wen-ting QIN Xi-ling NIU Xiang-li LU Feng-hua SHI De-shun 《中国畜牧兽医》2016,43(8):1975-1982
In this study,the CDS sequence of buffalo Keap1 gene was cloned and analyzed,then its expression pattern in different tissues was also investigated.A pair of primers of buffalo Keap1 gene was designed based on the nucleotide sequence of Bos taurus Keap1 gene from GenBank,and then the buffalo Keap1 gene was amplified.Using the bioinformation techniques,the gene sequence and the protein structure were analyzed.The expression level of Keap1 gene in different tissues were detected with Real-time quantitative PCR.The results showed that the length of buffalo Keap1 gene coding sequence was 1 875 bp and encoded 624 amino acids.The multiple sequence alignment results showed that buffalo Keap1 gene shared 99%,96%,92% and 90% of similar nucleotide sequence with that of Bos taurus,Ovis aries,Sus scrofa and Homo sapiens,respectively.And the phyogenetic tree also showed the conservatism between several different species.The second structure of buffalo Keap1 protein was predicted as 24 alpha regions,40 beta regions,38 turn regions and 27 coil regions.In addition,the results of Real-time quantitative PCR showed that Keap1 mRNA exists in all seven tissues,but the most abundant expression was in heart and the minimal expression was in liver and spleen.The results provided an foundation for further study of Keap1-Nrf2-ARE signal pathway,for enhancing the ability of antioxidant of buffalo embryo in vitro culture. 相似文献
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动物医学专业不仅要求学生掌握扎实的理论知识,还需要具有丰富的临床实践经验。为了实现该目标,除了要求授课教师对专业知识融会贯通,更需要拥有大量典型的临床病例资料。以安徽农业大学动物医院和畜禽疾病诊断中心为平台,收集大量宝贵的临床病例资料和最新的临床诊疗技术,构建了动物医学临床教学数据库,使得诊疗动物种类多样化,案例信息全面化、系统化。该数据库的建成和应用,为临床教师提供了丰富的教学素材和资料,亦提升了学生的学习兴趣,促进了动物医学专业的教育教学发展。 相似文献
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半夏种茎规格对产量、品质及植株的影响 总被引:1,自引:0,他引:1
在喀斯特温和气候区,采取小区试验方法,按半夏[Pinellia ternate(Thunb.)Breit.]种茎直径≥2.0cm、1.5 cm≤种茎直径<2.0 cm、1.0 cm≤种茎直径<1.5 cm、种茎直径<1.0 cm 4种规格种植,以块茎鲜重产量、总生物碱含量与种茎规格大、中、小的关系为考察依据,研究半夏不同规格种茎种植对块茎产量和总生物碱含量的影响。结果显示,随着种茎规格的增大,半夏块茎鲜重产量呈直线上升,以种茎直径≥2.0cm的产量最高,为2.44 kg/m2。净产量以1.5≤种茎直径<2.0 cm的最高,为0.98 kg/m2;总生物碱含量以1.0 cm≤种茎直径<1.5 cm的最高,为0.302 mg/g,二者均呈开口向下的抛物线变化。考虑到兼顾块茎产量和总生物碱含量,半夏种植以种茎直径为1.0~1.5 cm的较好。 相似文献
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陈小磊 《农业图书情报学刊》2013,25(4)
以探讨个性化服务的理论依据、图书馆个性化服务的内涵外延、特征、类型、内容等理论问题为基础,深入研究个性化服务的多种模式:分类定制服务、信息推送服务、智能代理服务和垂直门户,如何应用到艺术院校图书馆的数字资源利用和服务中. 相似文献
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CHEN Jia-qi JI Xin-qin DUAN Zhi-qiang WEN Ming RUAN Yong LEI Yun HOU Ping 《中国畜牧兽医》2016,43(2):296-303
To investigate the epidemic situation of H6N6 subtype avian influenza virus (AIV) in Guizhou province,A/duck/Guizhou/013/2014 was isolated from Sansui duck in live poultry market of Guizhou in 2014,the hemagglutinin (HA) and neuraminidase (NA) genes of DK/GZ/14 were subjected to clone and sequence analysis.The results showed that HA gene had the highest nucleotide homologies (97.5%) with the duck-origin H6N6 subtype AIV isolated from Eastern China in 2009,and the strains of HA gene proteolytic cleavage sites was P-Q-I-E-T-R-G,which accordeol with the molecular characteristic of low pathogenic AIV (LPAIV).However,NA gene of A/duck/Guizhou/013/2014 had the highest nucleotide homologies (98.2%) with the duck-origin H6N6 subtype AIV isolated from Fujian in 2007.The phylogenetic tree showed that A/duck/Guizhou/013/2014 and Hunan strains located in the same branch,while three duck-origin H6N6 subtype AIV isolated from Guizhou in 2007 and A/duck/Guizhou/013/2014 located in the different branch for HA and NA genes in genetic evolution,which suggested that A/duck/Guizhou/013/2014 was far with the local H6N6 subtype.The results also clearly indicated that duck-origin H6N6 subtype AIV had genetic diversity in duck population in Guizhou. 相似文献