遮阳网在鹤望兰生产上的应用

通过实测资料说明：遮阳网使棚内０．５ｍ及以下气层降温,０．５ｍ气温平均降低０．３℃,０．１ｍ平均降低０．９℃,０ｃｍ,５ｃｍ,１０ｃｍ土温平均降低５．０℃,３．１℃和２．６℃。相对浊度基本为适宜生长范围。而网外及棚内叶面喷水使生态环境更佳。     

红果冬青扦插育苗技术的研究

以红果冬青半木质化枝条为材料,研究了不同基质配方和外源激素处理对其生根的影响。结果表明：红果冬青插条在200mg／L的NAA溶液中浸泡3h后,再扦插在珍珠岩和泥炭土（容积比1：3）的基质中,插条的生根率和生根数最大,分别为91．6％和8．2。     

谷氨酰胺对断奶仔猪生长性能的影响

选取21日龄断奶杜大长仔猪60头(体重接近5 kg),按试验要求随机分成2组,每组设3个重复,每个重复10头.研究饲粮中添加1%谷氨酰胺对断奶仔猪生长的影响.结果表明,试验组在第9 d后腹泻率明显下降,而对照组在整个过程中的腹泻率较高,断奶8～11 d一直维持50%～60%的腹泻率,另外腹泻延续的时间较长,断奶12～15 d仍有20%～30%的腹泻率.断奶1～10 d,试验组料重比比对照降低了12.05%(P＜0.05).断奶11～20 d,试验组日增重比对照组提高27.75%(P＜0.05),试验组采食量、料重比与对照组相近.断奶10 d,试验组血清尿素氮水平比对照组低17.36%(P＞0.05),但对血清总蛋白和血清白蛋白水平无明显影响.断奶20 d,谷氨酰胺对血清尿素氮和总蛋白有一定影响,试验组血清尿素氮水平比对照组低4.26%(P＞0.05),血清总蛋白水平比对照组高18.70%(P＞0.05),谷氨酰胺对血清白蛋白水平无明显影响.饲粮添加谷氨酰胺对T3,T4水平无明显影响,但对生长激素水平有一定的提高作用.     

氮肥运筹对优质小麦旗叶硝酸还原酶活性的影响

选择不同追肥量和追肥时期 ,研究了氮肥运筹对优质小麦旗叶展开后NR活性和光合速率的影响。结果表明 ,旗叶全展后 ,2个品种NR活性变化趋势基本一致 ,开花期达到高峰 ,以后逐渐下降 ;拔节期施肥的NR活性显著高于开花期施肥处理 ,且开花期施肥的NR活性在开花以后下降缓慢 ;拔节期施肥可明显促进开花期的光合速率。泰山 2 1号拔节期施中肥和高肥 ,可保持整个生育期的光合速率处于较高水平 ,而泰山 2 2号中肥量处理 ( 9z1 ,9z2 )的光合速率则处于较高水平。Yg1、Yz1、 9z1、 9z2处理不仅NR活性高 ,而且光合速率高且平稳。     

Development of an Indirect Enzyme-Linked Immunosorbent Assay for Seromonitoring Contagious Bovine Pleuropneumonia Using Recombinant Lipoprotein LppQ of Mycoplasma mycoides subsp mycoides SC as Antigen

Mycoplasma mycoides subsp mycoides SC （MmmSC） is the etiological agent of contagious bovine pleuropneumonia （CBPP）. The lipoprotein LppQ encoded by lppQ gene is specific to MmmSC and is found in the type strain and in field strains isolated in Europe, Africa, and Australia, as well as in vaccine strains. No serological cross-reactions were observed with the related mycoplasmas of the Mycoplasma mycoides cluster. The N-terminal domain of the mature lipoprotein LppQ is hydrophilic, and it induces a strong, specific, early, and persistent immune response in naturally and experimentally infected animals. Mycoplasma-specific TGA （Trp） codons are utilized as stop codons in most other organisms. The lppQ N-terminal fragment from MmmSC HVRI X strain, the Chinese strain for CF antigen production, was mutated with one-step overlapping extension PCR. Sequence analysis confirmed the successful mutation from A to G in codon 198 in the lppQ gene. The fragment containing the mutation site was subcloned into the pET32a expression vector. The recombinant protein with molecular weight of 42 kDa was purified using the Ni-NTA His.Bind purification kit, with a purity of up to 95%. Western blot indicated that the standard positive serum of CBPP could react with the recombinant protein. The purified protein was diluted to 0.35 μg mL^-1, and coated to microtiter enzyme-linked immunosorbent assay （ELISA） plates. Indirect ELISA reaction conditions were optimized. The value of P/N was determined to be 4.8 （0.934/0.193）, the sensitivity to be 95.8% （46/48）, and the specificity to be 98.9% （161/163）. 3 817 cattle serum samples from three different provinces were detected by the indirect ELISA and CFT. The Kappa value is 0.63, which is middle or high agreement between the two methods.     

杭嘉湖水网平原农业非点源污染的综合调查和评价

首次对杭嘉湖水网平原地区开展了农业非点源污染的详细分类调查,并采用等标排放量的评价方法对各污染源进行了综合评价.结果表明,畜禽粪尿污染、乡镇生活污染和地表径流污染对水体污染的负荷贡献率分别为43.81%、29.91%和22.43%,是制约该区农业和环境可持续发展的三大关键污染因子.     

Effect of concentrated decoction of Chinese herbal compound Buyanghuanwutang on cAMP-PKA-CREB signaling pathway in hippocampus of rats with vascular dementia

AIM: To evaluate the role of concentrated decoction of Chinese herbal compound Buyanghuanwutang (BYHWT) in cyclic adenosine monophosphate(cAMP)-protein kinase A(PKA)-cAMP response element-binding protein(CREB) signaling pathway in hippocampus of rats with vascular dementia (VD). METHODS: The rats were randomly divided into sham operation group (sham-operated rats treated with normal saline), VD model group (VD rats treated with normal saline), BYHWT treatment group (VD rats treated with BYHWT) and nimodipment treating group (VD rats treated with nimodipine). The rat model of VD was build by the method of four-vessel occlusion. The rats in all 4 groups were administered with the corresponding reagents for successive 30 days. The content of cAMP was measured by radioimmunoassay. The expression of PKA catalytic subunit (PKAc) was observed by Western blotting. The changes of DNA-binding activity of CREB in rat hippocampus were detected by electrophoretic mobility shift assay. RESULTS: The content of cAMP, the expression of PKAc and the DNA-bingding activity of CREB in the hippocampus of VD rats were lower than those in the hippocampus of sham-operated rats (P<0.01). The above indexes in both nimodipine treatment group and BYHWT treatment group were definitely higher than those in VD model group (P<0.01). CONCLUSION: BYHWT increases the content of cAMP, the expression of PKAc and the DNA-binding activity of CREB in VD rat hippocampus, thus strengthening the cAMP-PKA-CREB signaling pathway.     

天津市无公害蔬菜产品“电子身份证”的建立与示范

天津市无公害蔬菜产品的质量安全不仅关系到蔬菜产业的健康发展,更关系到市民的健康与天津市农产品市场在全国的形象。为提高天津市蔬菜质量安全,有效增强政府对农产品质量监管的力度,提升品牌形象,增强产品竞争力,促进农民增收,农业主管部门启动了“天津市无公害蔬菜电子身份证的建立与示范”项目。该项目以创建天津市蔬菜安全生产和质量追溯系统为基础,从蔬菜基地安全生产技术、管理规范、市场准入及产品质量全程监测系统等方面进行研究和示范,积极探索“从农田到餐桌”的全程监管新模式,为打造放心蔬菜、品牌蔬菜提供可靠的技术支撑。     

工业区河涌污染与生态危害评价——以佛山三水大塱涡涌为例

在对工业区大塱涡涌水质及底泥重金属进行调查与分析的基础上,评价了底泥重金属潜在生态危害。结果表明,大塱涡涌水体COD、氨氮、总磷、DO与重金属Cr均超过地表水Ⅴ类水体标准,属于劣Ⅴ类水体;底泥重金属Cu、Zn与Cd平均含量超出土壤环境质量三级标准,且重金属Cu、Zn、Pb平均含量超过农用污泥污染物控制标准,该河段疏浚底泥不宜作为肥料直接施用于农田;大塱涡涌表层底泥重金属的潜在生态危害程度总体为强,几种重金属潜在生态危害平均程度从大到小为：Cd〉Cu〉Pb〉Zn〉Cr。     

转化桑叶的毛头鬼伞子实体的降血糖作用

研究了转化桑叶的毛头鬼伞(Coprinus comatus)子实体对四氧嘧啶诱导的糖尿病小鼠体重、血糖、血清胰岛素、肝糖原、果糖胺、血脂代谢、体内氧化压力、肝脏中糖代谢酶活性等的影响.转化桑叶的鸡腿菇子实体提高了单一毛头鬼伞子实体或桑叶的降血糖作用,促进了胰岛素分泌,降低了体内氧化压力,提高了糖代谢的酶活性,但对血脂调节作用不显著.