辐射百合雄性不育突变体的RAPD分析

近年来,分子标记辅助育种取得了较大进展,各种标记方法的广泛使用极大地加快了新品种的选育速度。随机引物扩增DNA多态性分子标记(RAPD)因其灵敏度高、速度快,可对整个基因组进行标记且费用和技术难度较低,易于应用而倍受青睐[1],已在植物亲缘关系与品种鉴定[2～4]、遗传多样性检测[5,6]、遗传图谱的构建与基因定位[7]、辅助选择育种[8,9]、育种材料的早期的选择、突变体的鉴定等方面得到了较多的应用[10～13]。由于突变体是植物基因组(达2 0×109bp)少数位点发生突变的结果,1个随机引物仅能检测基因组的极少部分,因而突变体的检测相对困…     

基于决策树的高寒湿地类型遥感分类方法研究

以索加-曲麻河区域为例,探讨了三江源区域高寒湿地遥感分类方法。利用TM 影像数据和DEM及缨帽变换后的亮度、绿度、湿度,以及归一化水体指数(NDWI)等复合识别指标,构建决策树模型,对研究区不同地类进行区分。然后通过与传统的最大似然法监督分类所得到的结果进行对比,结果表明:利用基于指数的决策树分类方法对高寒湿地类型进行分类,较传统的最大似然法监督分类总体精度提高12.05%;总体kappa系数提高0.140 7; 对于河流、湖泊、沼泽、滩地等湿地类型,生产者精度和用户精度分别提高了6.06%, 6.25%; 0.12%, 3.13%; 6.99%, 25.00%;6.12%, 28.13%, 比监督分类均有明显的提高。证明基于指数的决策树分类方法是高寒区域湿地遥感分类的一种有效手段。     

Hematologic Studies of Rinderpest-Infected Swine

Complete hematologic studies on swine inoculated with virulent rinderpest virus are reported. There was a 67 per cent reduction in total leukocyte counts on the third day postinoculation; counts returned to normal by the eighth day. The remainder of the hematologic values showed no significant changes. There was a febrile response at 3 days postinoculation; by the eighth day, temperature had returned to normal. Other clinical signs were less conspicuous and consisted of anorexia, stiff gait, and arched back occurring only on the third and fourth days after inoculation. The immunity of previously inoculated swine was challenged with virulent rinderpest virus 23 days after initial infection and no abnormal change in hemograms or body temperature resulted. The rinderpest-inoculated groups developed antibodies as demonstrated by the virus neutralization tests in rabbits.     

Scrapie I. Transmission and Pathogenesis

Homogenized brain, extracts, and residues of brain from normal and scrapie sheep were inoculated into 116 sheep. Of 72 sheep inoculated with scrapie material 27 developed the disease, whereas four of 44 inoculated with `normal' brain material showed symptoms similar to those of scrapie. The scrapie agent survived extraction for 18 hours with diethyl ether followed by water for 24 hours. Similarly the agent survived extraction in a Soxhlet apparatus with 95 per cent ethanol containing 2.0 per cent 4 M HC1 for 24 hours.

There was no evidence of demyelination in sections of brain and spinal cord supporting the view that allergic encephalomyelitis is not responsible for the scrapie symptoms. Liver function, as measured by the bromsulfalein test, remained normal until just before death. These results preclude that liver dysfunction contributes to the disease but do not exclude the possibilities of other metabolic derangements.

     

Effect of Ampicillin on E. Coli of Swine Origin

The in vitro susceptibility of 103 cultures of E. coli isolated from scouring and nonscouring pigs, and four cultures of Salmonella isolated from a case of necrotic enteritis was tested against Ampicillin contained in nutrient broth at concentrations of 0, 0.1, 1.0 and 5.0 uG per ml. of the medium. All but three cultures of E. coli were found to be susceptible to 5.0 uG/ml., all Salmonella isolates were also susceptible to this concentration of the antibiotic. Susceptibility of E. coli was also tested by plating dilutions of fecal samples obtained from either a scouring or a nonscouring pig, with E.M.B. agar containing 0, 0.1, 1.0, 2.5, 5.0 and 10.0 uG Ampicillin per ml. of the medium. No difference in the growth of E. coli was observed at 0, 0.1 and 1.0 uG concentrations. The three higher concentrations of the antibiotic inhibited the growth of E. coli proportional to the amount of Ampicillin in each concentration.

Ampicillin proved very effective in alleviating the symptoms of hemorrhagic enteritis in a 11-week old pig. The disappearance of scours was associated with the replacement of the previously existing sero-biotypes of fecal E. coliwith another aberrant type of E.coli which produced H₂S. No Ampicillin resistant strains of E. coli emerged following treatment of the animal with this antibiotic.

     

Isolation of Western Equine Encephalomyelitis (Wee) Virus From Mosquitoes In Saskatchewan, 1962

Saskatchewan, in the summer of 1962, was the scene of an extensive outbreak of western equine encephalomyelitis (WEE) in horses. The results of mosquito survey work showed Culiseta inornata and Culex tarsalis respectively to be the two most abundant mosquito species during midsummer. These species are those reported to be most commonly associated with outbreaks of WEE. Five hundred and sixty-four pools of mosquitoes were examined for the presence of WEE virus. Six pools, three of C. tarsalis and one each of C. inornata, Aedes flavescens and Aedes dorsalis, yielded WEE virus. Positive mosquitoes were from St. Walburg (C. inornata), Saskatoon (C. tarsalis — two, A. dorsalis — one), Outlook (C. tarsalis) and Kisbey (A. flavescens).     

基于氮元素循环利用的鱼粪生化处理工艺研究

为探究鱼菜共生系统中适宜氮元素高效循环利用的鱼粪生化处理工艺,设置厌氧和好氧两种工艺对鱼粪进行生化处理,每种工艺设置三个反应温度,三个鱼粪TS浓度,研究不同工艺处理后鱼粪液中氮元素变化规律以及鱼粪液对生菜生长的影响。结果表明,经厌氧处理后的鱼粪液铵硝比增大,经好氧处理后的鱼粪液铵硝比降至1.0以下,TS浓度3.0-3.5%的鱼粪经35.0℃好氧处理后水培生菜的生长速率最优,说明最适宜生菜生长的鱼粪生化处理工艺为：原鱼粪TS浓度3.0-3.5%,经35.0℃好氧工艺处理10天。     

市售猪肉及其组织中"瘦肉精"残留调查

采用德国r-Biopharm拜发公司试剂盒，用竞争性EUSA法对某市13个菜场共抽取40份瘦肉、30份猪肝、18份猪肾脏、30份猪肺、15份猪骨和30份猪血、30份猪毛，总计样品进行了瘦肉精残留检测。结果表明，在抽检的193份样品中共有38份瘦肉精含量大于1．0ng／g，占总数的19．7％，其中猪肝与猪毛的检出率最高。同时在瘦肉精含量大于1．0ng／g的样品中抽取9份，用GC／MS／MS法确证，结果与ELISA法相符。     

工程制图课程思政的实践与思考——以一堂课程思政示范课为例

课程思政是当前高校思想政治教育工作的新模式。本文以一堂工程制图课程思政示范课为例,讲解机件形状表达方法中的剖视图。首先构建了工程制图课程思政建设的总体框架,充分挖掘教学知识点中的思政元素,通过案例实施探讨知识点与思政元素的有机融入方式,并通过学生匿名问卷调查和教师代表的听课反馈来评估课程思政的教学成效。最后提出了课程思政建设中的几点思考。