Strategies and priorities in field collections for ex situ conservation: the case of the Israel Plant Gene Bank

The ex situ collection of the Israel Plant Gene Bank (IGB) aims to encompass the rich local flora and its genetic diversity with an emphasis on crop wild relatives. However, to properly establish a core collection, collection efforts must be prioritized and strategized. We previously classified local plant genetic resources into four priority groups that assisted in strategizing the collection activities. The following years of intensive collection activity yielded over 4200 banked accessions. However, these do not necessarily represent the distribution range of the target species for collection (TSC) and consequently, their genetic diversity. To best cover the latter, the collecting area was divided into botanical districts and the magnitude of the collection was determined according to prioritization group, e.g., a wild relative of an agricultural crop with a vast distribution range should be represented by a larger number of banked accessions than one with a smaller range. Continuous evaluation of specific needs shapes the collection scheme of the IGB to maximize collection efforts, better represent the presumed genetic diversity of TSC, and establish its core collection.     

液相色谱-串联质谱法同时检测鸡肉中61种抗菌药物

为建立固相萃取结合液相色谱-串联质谱同时测定鸡肉中磺胺类、喹诺酮类、大环内酯类药物残留的分析方法,通过准确称取2 g鸡肉样品,经PBS缓冲液提取,以Agilent Pleax PCX固相萃取柱净化,最后以液相色谱-串联质谱进行检测.结果显示:在最优试验条件下测得磺胺类、喹诺酮类、大环内酯类抗菌药物线性良好,相关系数(R...     

氮肥去向的研究--Ⅰ.稻田土壤中氮肥的去向

氮肥施入土壤后的去向,直接关系到作物增产和环境保护,是农业和环境科学研究中的基本资料。对氮肥去向的研究,以在田间条件下进行的意义比较大。因此,1978-1980年,我们在华东地区三种不同土壤上,采用田间微区¹⁵N示踪的方法,分别测定了施用于水稻和小麦的几种常用氮肥的去向,并以尿素为重点,研究了施肥方法、施肥时期、土壤水分状况以及硝化抑制剂等对氮肥去向的影响。所得结果将分别整理。本文是稻田试验方面的初步总结。     

超微图像三维重建技术在治蝗农药研究中应用

传统的治蝗农药研究方法存在着研制周期长、实验结果不直观、无法定量研究等问题.基于连续组织超微切片图像的三维重建技术是一种重要的形态学研究方法,可建立生物农药与蝗虫互作组织的三维模型来模拟蝗虫组织器官与生物农药的相互作用过程,该技术在阐明蝗虫组织结构与生理功能间关系及生物农药研究等方面具有重要意义.为此,分析了该项技术在治蝗农药研究中的应用意义.其中,重点介绍了图像保边降噪模型、自适应图像分割、三维混合绘制等关键技术的研究进展.     

植物免疫诱抗剂的作用机理和应用研究进展

植物免疫诱抗剂除了能诱导植物免疫反应以使植物获得或提高对病菌的抗性外,也可以激发植物体内代谢调控过程,促进植物生长,增加作物产量。植物免疫诱抗剂可以诱导植物产生系统获得抗性以使植物对病原菌产生广泛、长期的抗性。本综述全面、系统地介绍了植物免疫诱抗剂的种类及其目前在生产、病害防治上的应用状况,阐述了其作用机理,并展望了植物免疫诱抗剂的应用前景。通过对植物免疫诱抗剂发展历史和应用现状的分析,旨在为植物免疫诱抗剂未来的开发和应用提供一定的理论和实践依据。     

白屈菜CmFAD2基因的克隆及植物转化载体构建

通过对白屈菜低温应答过程的转录组分析发现膜脂不饱和化相关基因的表达在一定过程中发生变化,脂肪酸去饱和酶基因FAD2在随温度的变化趋势为正V型,且表达量变化显著。利用NCBI等在线软件对序列进行相关生物学信息分析,并对白屈菜FAD家族成员FAD2基因的完整开放阅读框(ORF)进行克隆,并命名为CmFAD2。选用克隆载体pMD-19-T,转化大肠杆菌DH5α,测序验证序列正确性及完整性。将目的基因与植物表达载体pRI-201-AN连接构建重组DNA pRI-201-AN-Cm FAD2,电击法转化农杆菌LBA4404,利用菌液PCR法验证成功。该基因可作为药用植物抗寒品种创制的候选基因。     

The performance of community organisers in promotion of community forestry in Leyte Province,Philippines

A study was conducted to assess the role and effectiveness of community organisers in supporting the development of people’s organisations in achieving community-based forest management objectives in Leyte Province Philippines. Community organisers were found to be effective in forming people’s organisations (POs), motivating people to participate in voluntary activities organised by POs and encouraging cohesiveness among PO members. Community organisers manage to raise the level of environmental awareness and knowledge of members of people’s organisations, develop leadership interest and skills, create various livelihood opportunities and provide direction and facilitate the establishment of large tree plantations. However, the short duration of community organisers’ contracts (typically two years) is insufficient to establish mature and cohesive POs prepared to assume management on their own, including the management of tree plantations. Further, lack of training and funding support, low wages, delayed payment of salaries and limited time to work with people’s organisations, as well as the pressure to produce tangible outputs such the establishment of large tree plantations, prevents them from placing greater emphasis on the development and empowerment of the people.     

仙草蜜抗氧化活性的研究

利用Fenton反应,研究了仙草蜜清除羟自由基的作用;采用邻苯三酚自氧化法,研究仙草蜜清除超氧阴离子自由基的作用。结果表明,仙草蜜具有清除羟自由基和超氧阴离子自由基的作用,且在实验的范围内,其清除能力随浓度的增大而增强。     

大豆蛋白质含量遗传变异特点及早世代选择效果的研究

利用５个亲本配制了３个杂交组合，研究其杂种后代Ｆ２－Ｆ４代蛋白质含量遗传变异的特点及早世代分组选择的效果。结果表明：（１）大豆蛋白质含量以基因的加性效应为主；但也存在一定的显性效应。（２）相关分析显示，中亲与后代的相关值比父本或母本与后代的相关值高，世代间的相关显著，相关值以邻近世代间较高；（３）蛋白质含量总的变异趋势是：组合间＞家系间＞家系内单株间；（４）在大豆杂交组合的早世代对单株蛋白质含量进行选择是有效的，而在Ｆ２的选择较在以后世代中选择更为有效。     

Unravelling pest infestation and biological control in low-input orchards: the case of apple blossom weevil

Low-input farming is an alternative production system that provides a great opportunity to disentangle the natural mechanisms regulating crop pests, since neither pests nor their natural enemies are disrupted by pesticides. Here, we use a key apple pest in Europe, the apple blossom weevil (Anthonomus pomorum), as a model case to unravel the factors driving pest infestation and its biological control in a low-input context, namely the cider apple orchards of NW Spain. We applied a holistic approach based on the complete life cycle of the pest and combined large-scale observation (23 orchards) with small-scale experimental assessment. Weevil attack (0.4–37.4% of flowers) increased with the proportion on apple trees in the immediate orchard neighbourhood and with semi-natural woody habitat in the surrounding landscape and decreased with tree distance to orchard edge and apple bloom level. Thus, the prevalence of the pest depended on the availability of the various resources required for foraging, egg-lying and overwintering. Three types of natural enemies supplied complementary pest control by preying on weevils at different stages in their life cycle: seven parasitoid species attacked immature weevils (6.4–81.5%), while the additive effects of birds and crawling arthropods were evident in terms of the removal of adult weevils (31–44%). We conclude that the effective biological control of A. pomorum can be achieved in low-input systems to maintain the pest at non-harmful levels, through combined management of the pest, its habitat and its natural enemies.