潘集区发展现代农业促进农民增收的现状、问题和对策

本文介绍了潘集区发展现代农业促进农民增收的现状,并针对其出现的问题及机遇,提出了相应的对策建议.     

新城疫病毒La Sota株HN基因的克隆与序列分析

旨在从分子生物学角度进一步研究新城疫病毒La Sota株HN基因,探究NDV La Sota HN基因的遗传变异情况。研究以试验室冻存的p NDV-HN为模版,根据Gen Bank中登录的NDV La Sota株序列设计引物扩增HN基因,将其克隆到pMD18-T载体后进行鉴定,对鉴定正确的重组质粒p MD-NDV-HN进行测序分析。应用生物信息学软件对新城疫病毒Lasota株HN基因进行系统进化树分析、氨基酸序列同源性分析、糖基化位点、蛋白结构跨膜区分析及磷酸化位点预测分析。核苷酸序列测定结果表明:HN基因的序列长度为1743bp,该基因的开放阅读框(Open Reading Frame,ORF)总长为1716 bp,编码571个氨基酸。生物信息学表明:试验获得的HN基因具有6个潜在糖基化位点及12个半胱氨酸残基,第5个潜在糖基化位点(508-510 aa)发生缺失及第123位半胱氨酸残基被色氨酸残基取代。La Sota株HN基因编码的蛋白质中有29个丝氨酸、10个酪氨酸和16个苏氨酸可能成为蛋白激酶磷酸化位点。将试验获得的La Sota株HN基因序列和推导的氨基酸序列与新城疫毒株的HN基因相应序列比较后发现,它们的核苷酸序列同源性和氨基酸序列同源性最高均可达到99%,表明HN基因在种属间具有较高的保守性。研究为新城疫病毒的分子病毒学研究奠定了基础。     

ANALYTIC SOLUTION OF CYLINDRICAL SHELL ROOF UNDER GENERAL TRANSVERSE LOCAL LOADINGS

This paper uses the method of mixed-type series to derive the analyticsolution of cylindrical shell roof,being simply supported transversely,arbitra-rily restrained longitudinally and subjected to gereral transverse local loads.This paper also presents the analytic expressions of the solutions for somekinds of local load.Some examples of this kind of shell roof subjected to ainlined single force near the middle of it with free lorgitudinal edges aregiven.The computation shows that the convergence of the solution is woll     

System Design for Leather Cavity XCT

This paper introduces a method of solving the problem of leather cavity measurement in shoe CAD/CAM by industrial CT technology. Concentrating on peculiarity and requirement of leather cavity measurement, this paper presents a X-ray industrial CT system scheme, which not only satisfy the requirement of leather cavity measurement but also can achieve general industrial CT. measurement. The achievement on data collection system and motion control of stepping motor is emphasized.     

A Multi-agent Fault Diagnosis System of Rotating Machinery Based on CBR

Recently,distributed intelligent systems based on multi-agent have been applied to many fields successfully.the multi-agent technique is introduced into the rotating machinery fault diagnosis system.With the combination of CBR technique,Basalstructure and development approach of the system are analyzed,and better cooperation among agents is realized.As a result,the system overcomes the limitation of single fault diagnosis method,and solves the contradictionbetween versatilityand adaptabilityof diagnosis software.Furthermore,a reference can be used for the research and development of multi-algorithmdiagnosis system of rotating machinery.The application in a factory's Networked Online Monitoring and Fault Diagnosis System for Turbine Fan shows that the system can diagnosefault rapidly and exactly.     

Resource Management in Application System

Application system generally refers to all kinds of computer information system. It aims at improving the sharing and application of the resource. The resource in application system usually means data resource. For computer management and maintenance, there are not only data resource, but also program resource and network resource. Effective management of this resource is key to ensure proper work of the application system. In this paper, the basic pattern and policy for resource management is put forward and a method to build and realize the resource layer that lies between database layer and application layer is to be found. This management layer, which skillfully connects the application system with resource and establishes good base for application and exploitation of system, is named as resource management system.     

Effect of Stitching on the In-plane Tensile Strength of Stitched Composite Laminates

Some additional effects of stitching on the in-plane mechanical properties of composite laminate is introduced while it was used to improve the interlaminar of plane mechanical properties of the composite laminate. The paper investigates the effect of stitching on in-plane tensile strength of composite laminates. The results show that the effect of stitching is dependent on the stitching parameters. The in-plane tensile strength of laminates is degraded with the decreasing of stitching space and step and increasing diameter of stitching thread. It is found that the initial damage of laminate increased with increasing of stitching parameters such as stitching density. This causes the decrease in the in-plane strength of composite laminate.     

Accomplishment of Elastic-plastic Analysis in Geotechnical FEA

In order to realize elastic-plastic analysis in geotechnical FEA, in this paper, firstly, the method how to realize elastic-plastic analysis in Super-Sap is discussed. Then, this paper stresses to introduce the principle and the steps used to achieve the accomplishment of elastic-plastic analysis in Super-Sap. Finally, an engineering practice is given to prove that the geotechnical FEA elastic-plastic analysis and visualized post-processing can come true by it rapidly.