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51.
Casper Lindegaard Anna B Frost Maj H Thomsen Claus Larsen Steen Honoré Hansen Pia Haubro Andersen 《Veterinary anaesthesia and analgesia》2010,37(2):186-195
ObjectiveTo describe the pharmacokinetics of intra-articularly (IA) administered morphine.Study designExperimental randomized, cross-over study.AnimalsEight adult healthy mixed breed horses aged 6.5 ± 2.3 (mean ± SD) years and weighing 535 ± 86 kg.MethodsUnilateral radiocarpal synovitis was induced by IA injection of 3 μg lipopolysaccharide (LPS) on two occasions (right and left radiocarpal joint, respectively) separated by a 3-week wash-out period. Treatments were administered 4 hours post-LPS-injection: Treatment IA; preservative free morphine IA (0.05 mg kg?1) plus saline intravenous (IV) and treatment IV; saline IA plus preservative free morphine IV (0.05 mg kg?1). Concentrations of morphine, morphine-3-glucuronide and morphine-6-glucuronide (M6G) were determined repeatedly in serum and synovial fluid (SF) by high-performance liquid chromatography mass spectrometry, at 2 and 4 hours and then at 4 hours intervals until 28 hours post-treatment.ResultsInjection of LPS elicited a marked and comparable synovitis in all LPS-injected radiocarpal joints. IA administered morphine was detectable in SF of all eight joints 24 hours post-treatment and in 6/8 joints 28 hours post-treatment. The terminal half-life of morphine in SF was estimated to be 2.6 hours. IA administration of morphine resulted in mean serum concentrations of morphine below 5 ng mL?1 from 2 to 28 hours after treatment.Conclusions and clinical relevanceIntra-articularly administered morphine remained within the joint for at least 24 hours. At the same time only very low serum concentrations of morphine and M6G were detected. The present results suggest that IA morphine at 0.05 mg kg?1 may be used for IA analgesia lasting at least 24 hours and give strong support to the theory that previously observed analgesic and anti-inflammatory effects of IA morphine in horses are most likely to be mediated peripherally. 相似文献
52.
Argentine ants Linepithema humile (Mayr), common pugnacious ants Anoplolepis custodiens (F. Smith) and cocktail ants Crematogaster peringueyi Emery are the main indirect ant pests in South African vineyards. These ants form mutualistic relationships with the vine mealybug Planococcus ficus (Signoret) (Hemiptera: Pseudococcidae), an economic phloem-feeding pest of vines that excretes honeydew. Ants feed on the honeydew from mealybugs and also affect predator-prey interactions by protecting them from attack by natural enemies. This consequently reduces the efficacy of predators and parasitic Hymenoptera in controlling P. ficus. Current strategies for ant control are limited to the application of long term residual insecticides that are detrimental to the environment, labour intensive to apply and potentially disruptive to biological control. Here, we report on the development of an alternative method of ant control using baits which are likely to show delayed toxicity to these ant species. Field bait preference assessments were carried out during spring, summer and autumn in three vineyards of the Cape Winelands region, Republic of South Africa during 2007/08. Preference/non-preference is the first behaviour an ant exhibits when encountering bait and this was measured in terms of numbers of ants at bait stations. Five toxicants comprising Gourmet ant bait (containing 0.5% boric acid as active ingredient and a honeydew mimic as an attractant), boric acid (0.5%), fipronil (0.0001%), fenoxycarb (0.5%) and spinosad (0.01%) all dissolved in 25% sucrose solution were tested against a 25% sucrose solution control. Gourmet ant bait was overall the most preferred bait during spring, summer and autumn, and on some occasions being significantly more preferable to ants than the control solution. 相似文献
53.
54.
Gustavson Kim Hansson Sophia V. van Beest Floris M. Fritt-Rasmussen Janne Lassen Pia Geertz-Hansen Ole Wegeberg Susse 《Water, air, and soil pollution》2020,231(9):1-13
Water, Air, &; Soil Pollution - The ecotoxicity of nickel depends on water quality characteristics such as pH and dissolved organic carbon. Bioavailability models to predict nickel toxicity have... 相似文献
55.
Intestinal establishment and reproduction of adult Trichinella spiralis, Trichinella nativa, Trichinella britovi and Trichinella pseudospiralis were examined as single species or mixed species infections in foxes. This is the first study of intestinal dynamics of Trichinella spp. in a carnivore model and the results suggest that the intestinal phase is relatively short as only very few worms were recovered 10 days post-inoculation (dpi). In mixed species infection with equal doses of T. nativa and T. spiralis, molecular typing demonstrated that 64% of the intestinal worms and 78% of the muscle larvae were T. nativa. Conversely, T. spiralis dominated in the mixed species infections with T. pseudospiralis, constituting 66% of the intestinal worms and 94% of the muscle larvae. Although, the individual recoveries of intestinal worms were only up to 5.6% on day 1, and up to 1.5% on day 4 post-infection, the muscle larvae establishment was comparable to other fox studies. Infectivity, measured as muscle larvae burden did not differ among the four species of Trichinella, which is in contrast to other models with mice, rats, pigs or herbivores. Although statistically significant differences in intestinal worm burdens were found for some days, no distinct species were recovered in consistently higher numbers than the others. 相似文献
56.
Previously described methods for the recovery of intestinal Trichinella worms from rodents are not feasible when applied in larger experimental animals such as foxes. In this study,worm recovery by standard technique of simple incubation of the intestine in saline was compared to embedment of the intestine in an agar gel. The small intestines of Trichinella spiralis infected foxes (4-5 days post inoculation) were slit lengthwise and the two corresponding halves were processed with one of the two incubation methods.Worms were recovered from all samples, and the total worm recovery ranged from 0.2-4.4% of the infection dose. The samples from the standard incubation were very unclear and time consuming to count compared with samples from the agar gel embedment,in which the intestinal debris were kept inside the agar. As the agar gel technique generally yielded higher numbers of worms than the corresponding standard incubation sample, it is with some optimisation, recommended for recovery of intestinal Trichinella worms from foxes. 相似文献
57.
Marntell S Nyman G Funkquist P Hedenstierna G 《Veterinary anaesthesia and analgesia》2005,32(2):83-93
OBJECTIVE: To study pulmonary gas exchange and cardiovascular responses to sedation achieved with romifidine and butorphanol (RB) alone, or combined with acepromazine, and during subsequent tiletamine-zolazepam anaesthesia in horses. ANIMALS: Six (four males and two females) healthy Standardbred trotters aged 3-12 years; mass 423-520 kg. STUDY DESIGN: Randomized, cross-over, experimental study. MATERIALS AND METHODS: Horses were anaesthetized on two occasions (with a minimum interval of 1 week) with intravenous (IV) tiletamine-zolazepam (Z; 1.4 mg kg(-1)) after pre-anaesthetic medication with IV romifidine (R; 0.1 mg kg(-1)) and butorphanol (B; 25 microg kg(-1) IV). At the first trial, horses were randomly allocated to receive (protocol ARBZ) or not to receive (protocol RBZ) acepromazine (A; 35 microg kg(-1)) intramuscularly (IM) 35 minutes before induction of anaesthesia. Each horse was placed in left lateral recumbency and, after tracheal intubation, allowed to breathe room air spontaneously. Respiratory and haemodynamic variables and ventilation-perfusion (; multiple inert gas elimination technique) ratios were determined in the conscious horse, after sedation and during anaesthesia. One- and two-way repeated-measures anova were used to identify within- and between-technique differences, respectively. RESULTS: During sedation with RB, arterial oxygen tension (PaO(2)) decreased compared to baseline and increased mismatch was evident; there was no O(2) diffusion limitation or increase in intrapulmonary shunt fraction identified. With ARB, PaO(2) and remained unaffected. During anaesthesia, intrapulmonary shunt occurred to the same extent in both protocols, and mismatching increased. This was less in the ARBZ group. Arterial O(2) tension decreased in both protocols, but was lower at 25 and 35 minutes of anaesthesia in RBZ than in ARBZ. During sedation, heart rate (HR) and cardiac output (Qt) were lower while arterial-mixed venous oxygen content differences and haemoglobin concentrations were higher in RBZ compared with ARBZ. Total systemic vascular resistance, mean systemic, and mean pulmonary arterial pressures were higher during anaesthesia with RBZ compared to ARBZ. CONCLUSIONS AND CLINICAL RELEVANCE: Acepromazine added to RB generally improved haemodynamic variables and arterial oxygenation during sedation and anaesthesia. Arterial oxygenation was impaired as a result of increased shunt and mismatch during anaesthesia, although acepromazine treatment reduced disturbances and falls in PaO(2) to some extent. Haemodynamic variables were closer to baseline during sedation and anaesthesia when horses received acepromazine. Acepromazine may confer advantages in healthy normovolaemic horses. 相似文献
58.
Temeyer KB Li AY Lohmeyer KH Chen AC Olafson PU Sanson DW Foil LD 《Veterinary parasitology》2008,154(3-4):300-310
Acetylcholinesterase (AChE) cDNA from individual field-collected diazinon-resistant horn flies was amplified by RT-PCR. Sequencing of the amplification products revealed that 8/12 of the diazinon-resistant horn flies contained a point mutation previously associated with resistance to organophosphates in house flies and Drosophila, strongly suggesting that this cDNA encodes the AChE that is the target site for organophosphate (OP) pesticide. The point mutation (G262A) resulted in a shift from glycine to alanine in the mature HiAChE amino acid sequence at position 262. Allele-specific PCR and RLFP assays were developed to diagnose the presence or absence of the G262A mutation in individual flies. Use of the allele-specific assays each demonstrated the presence of the G262A mutation in 10 of 12 individual field-collected flies, demonstrating higher sensitivity than direct sequencing of RT-PCR amplification products. The G262A mutation was found in additional fly populations previously characterized as OP-resistant, further supporting that this AChE is the target site for OP pesticide. The allele-specific assay is a useful tool for quantitative assay of the resistance allele in horn fly populations. 相似文献
59.
Asian Mega deltas (AMDs) are among the world´s most important rice-growing areas and vital for food security. This review describes the major environmental and agricultural characteristics as well as current and future threats to food production and livelihood, using three AMDs as examples, namely the Ganges–Brahmaputra delta in Bangladesh, the Ayeyarwady delta in Myanmar and the Mekong River delta in Vietnam. In addition, we analysed the most widely used agricultural adaptation methods in these areas. The major threat to agriculture is salinity intrusion and water scarcity, which consequently leads to a shortage of irrigation water and yield reduction. The most common applied agricultural adaptation strategies are the use of salt-tolerant varieties and the adjustment of cropping calendars, as well as the use of water-saving technologies. It was found that there are many concepts that address individual problems, but a comprehensive and integrated concept for delta regions to adapt to climate change is still missing. 相似文献
60.
Tytti Niemel? Tuomas Virén Jukka Liukkonen David Argüelles Nikae C R te Moller Pia H Puhakka Jukka S Jurvelin Riitta-Mari Tulamo Juha T?yr?s 《Acta veterinaria Scandinavica》2014,56(1):3